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Protein

ADP-ribosylation factor-like protein 1

Gene

Arl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein. Can activate phospholipase D with very low efficiency (By similarity). Important for normal function of the Golgi apparatus.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Magnesium1 Publication
Metal bindingi46 – 461Magnesium; via carbonyl oxygen1 Publication
Metal bindingi48 – 481Magnesium1 Publication
Binding sitei70 – 701GTP; via amide nitrogen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP
Nucleotide bindingi45 – 484GTP
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi126 – 1294GTP
Nucleotide bindingi160 – 1612GTP

GO - Molecular functioni

GO - Biological processi

  • activation of phospholipase D activity Source: UniProtKB
  • Golgi organization Source: RGD
  • Golgi vesicle transport Source: RGD
  • protein localization to Golgi apparatus Source: UniProtKB
  • protein transport Source: InterPro
  • retrograde transport, endosome to Golgi Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
  • toxin metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 1
Gene namesi
Name:Arl1
Synonyms:Arf7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621326. Arl1.

Subcellular locationi

  • Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: GOC
  • extracellular exosome Source: Ensembl
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Abolishes association with the Golgi apparatus. 1 Publication
Mutagenesisi31 – 311T → N: Accumulation of GDP-bound form. 1 Publication
Mutagenesisi71 – 711Q → L: Accumulation of GTP-bound form. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 181180ADP-ribosylation factor-like protein 1PRO_0000207452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP61212.
PRIDEiP61212.

PTM databases

iPTMnetiP61212.

Expressioni

Gene expression databases

GenevisibleiP61212. RN.

Interactioni

Subunit structurei

The GTP-bound form interacts with RGPD8 (By similarity). The GTP-bound form directly interacts with ARFIP2; this interaction leads to an increase in the amount of bound GTP at steady state level (By similarity). Binds to SCOC, preferentially in its GTP-bound form. May interact with UNC119 (By similarity). The GTP-bound form interacts with GOLGA1 and GOLGA4.By similarity2 Publications

Protein-protein interaction databases

IntActiP61212. 2 interactions.
MINTiMINT-4565645.
STRINGi10116.ENSRNOP00000007623.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 236Combined sources
Helixi30 – 367Combined sources
Beta strandi49 – 6012Combined sources
Beta strandi62 – 687Combined sources
Helixi72 – 8110Combined sources
Beta strandi87 – 959Combined sources
Helixi100 – 11213Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1438Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4AX-ray2.30A/B/C/D16-180[»]
ProteinModelPortaliP61212.
SMRiP61212. Positions 16-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61212.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0072. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61212.
KOiK07942.
OMAiDMEGCMS.
OrthoDBiEOG7JDR02.
PhylomeDBiP61212.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG
60 70 80 90 100
FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI
110 120 130 140 150
GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTPSEMA NALGLPALKD
160 170 180
RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q
Length:181
Mass (Da):20,412
Last modified:May 10, 2004 - v1
Checksum:i3DFC4CC8ED632FEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76920 mRNA. Translation: CAA54245.1.
U12402 mRNA. Translation: AAA20668.1.
BC061553 mRNA. Translation: AAH61553.1.
PIRiA54022.
RefSeqiNP_071780.1. NM_022385.3.
UniGeneiRn.3065.

Genome annotation databases

EnsembliENSRNOT00000007623; ENSRNOP00000007623; ENSRNOG00000005763.
GeneIDi64187.
KEGGirno:64187.
UCSCiRGD:621326. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76920 mRNA. Translation: CAA54245.1.
U12402 mRNA. Translation: AAA20668.1.
BC061553 mRNA. Translation: AAH61553.1.
PIRiA54022.
RefSeqiNP_071780.1. NM_022385.3.
UniGeneiRn.3065.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4AX-ray2.30A/B/C/D16-180[»]
ProteinModelPortaliP61212.
SMRiP61212. Positions 16-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP61212. 2 interactions.
MINTiMINT-4565645.
STRINGi10116.ENSRNOP00000007623.

PTM databases

iPTMnetiP61212.

Proteomic databases

PaxDbiP61212.
PRIDEiP61212.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007623; ENSRNOP00000007623; ENSRNOG00000005763.
GeneIDi64187.
KEGGirno:64187.
UCSCiRGD:621326. rat.

Organism-specific databases

CTDi400.
RGDi621326. Arl1.

Phylogenomic databases

eggNOGiKOG0072. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61212.
KOiK07942.
OMAiDMEGCMS.
OrthoDBiEOG7JDR02.
PhylomeDBiP61212.

Enzyme and pathway databases

ReactomeiR-RNO-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

EvolutionaryTraceiP61212.
PROiP61212.

Gene expression databases

GenevisibleiP61212. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of two novel ADP-ribosylation factor-like proteins and characterization of their differential expression in 3T3-L1 cells."
    Schuermann A., Breiner M., Becker W., Huppertz C., Kaninulainen H., Kentrup H., Joost H.-G.
    J. Biol. Chem. 269:15683-15688(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Adipose tissue.
  2. Lowe S.L., Hong W.J.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  4. "Regulation of Golgi structure and function by ARF-like protein 1 (Arl1)."
    Lu L., Horstmann H., Ng C., Hong W.
    J. Cell Sci. 114:4543-4555(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-2; THR-31 AND GLN-71.
  5. "Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 onto the Golgi."
    Lu L., Hong W.
    Mol. Biol. Cell 14:3767-3781(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GOLGA1 AND GOLGA4.
  6. "Structural basis for recruitment of GRIP domain golgin-245 by small GTPase Arl1."
    Wu M., Lu L., Hong W., Song H.
    Nat. Struct. Mol. Biol. 11:86-94(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 16-180 IN COMPLEX WITH GDPNP; MAGNESIUM AND GOLGA4.

Entry informationi

Entry nameiARL1_RAT
AccessioniPrimary (citable) accession number: P61212
Secondary accession number(s): P41276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.