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Protein

ADP-ribosylation factor 1

Gene

Arf79F

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; has a role in Golgi organization and may modulate vesicle budding and uncoating within the Golgi apparatus (Probable). Has a role in eye development (PubMed:21976699). Required for cleavage furrow ingression in embryonic cells (PubMed:27535433).Curated2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • ARF protein signal transduction Source: FlyBase
  • compound eye development Source: FlyBase
  • compound eye morphogenesis Source: UniProtKB
  • endosomal transport Source: FlyBase
  • male meiosis cytokinesis Source: FlyBase
  • mitotic cleavage furrow ingression Source: UniProtKB
  • neurotransmitter secretion Source: FlyBase
  • positive regulation of endocytosis Source: FlyBase
  • post-Golgi vesicle-mediated transport Source: FlyBase
  • protein transport Source: UniProtKB-KW
  • regulation of hematopoietic stem cell differentiation Source: FlyBase
  • regulation of lipid storage Source: FlyBase
  • synaptic vesicle endocytosis Source: FlyBase

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1660499. Synthesis of PIPs at the plasma membrane.
R-DME-1660514. Synthesis of PIPs at the Golgi membrane.
R-DME-421837. Clathrin derived vesicle budding.
R-DME-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:Arf79F
Synonyms:ARF1
ORF Names:CG8385
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0010348. Arf79F.

Subcellular locationi

  • Golgi apparatus 1 Publication
  • Cytoplasmcytosol 1 Publication

  • Note: Localization to the Golgi is dependent on Asap.1 Publication

GO - Cellular componenti

  • apical part of cell Source: UniProtKB
  • cleavage furrow Source: FlyBase
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB
  • Golgi stack Source: FlyBase
  • presynapse Source: GOC

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Conditional RNAi-mediated knockdown in the eye results in aberrant compound eye morphogenesis (PubMed:21976699). Maternal RNAi-mediated knockdown results in abnormal aggregation of Golgi apparatus and disrupted cleavage furrow ingression in early embryos (PubMed:27535433).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46I → D: Abnormal aggregation of Golgi apparatus and disrupted cleavage furrow ingression in early embryos. May be insensitive to Asap activation. 1 Publication1
Mutagenesisi52N → A: No defect in Golgi apparatus and cleavage furrow ingression in early embryos. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074412 – 182ADP-ribosylation factor 1Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP61209.
PRIDEiP61209.

Expressioni

Gene expression databases

BgeeiFBgn0010348.
ExpressionAtlasiP61209. differential.
GenevisibleiP61209. DM.

Interactioni

Protein-protein interaction databases

BioGridi65734. 47 interactors.
IntActiP61209. 8 interactors.
MINTiMINT-970074.
STRINGi7227.FBpp0289184.

Structurei

3D structure databases

ProteinModelPortaliP61209.
SMRiP61209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
InParanoidiP61209.
KOiK07937.
OMAiHYYSNTD.
OrthoDBiEOG091G0S7S.
PhylomeDBiP61209.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
PfamiView protein in Pfam
PF00025. Arf. 1 hit.
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51417. ARF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNVFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERI
110 120 130 140 150
GEAREELMRM LAEDELRDAV LLIFANKQDL PNAMNAAEIT DKLGLHSLRN
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLKNA NR
Length:182
Mass (Da):20,688
Last modified:January 23, 2007 - v2
Checksum:i9E87E24DFAEA3674
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62079 Genomic DNA. Translation: AAB27066.1.
AE014296 Genomic DNA. Translation: AAF51871.1.
AE014296 Genomic DNA. Translation: AAF51872.1.
AE014296 Genomic DNA. Translation: AAF51873.1.
AE014296 Genomic DNA. Translation: AAF51874.1.
AE014296 Genomic DNA. Translation: AAN12207.1.
AE014296 Genomic DNA. Translation: ABW08583.1.
AE014296 Genomic DNA. Translation: ABW08584.2.
AY060375 mRNA. Translation: AAL25414.1.
PIRiA49520.
RefSeqiNP_001097667.1. NM_001104197.3.
NP_001097668.2. NM_001104198.2.
NP_001262225.1. NM_001275296.1.
NP_476955.1. NM_057607.6.
NP_730757.1. NM_168970.4.
NP_730758.1. NM_168971.4.
NP_730759.1. NM_168972.5.
NP_730760.1. NM_168973.3.
UniGeneiDm.6446.

Genome annotation databases

EnsemblMetazoaiFBtr0078571; FBpp0078222; FBgn0010348.
FBtr0078573; FBpp0078224; FBgn0010348.
FBtr0078574; FBpp0078225; FBgn0010348.
FBtr0078575; FBpp0078226; FBgn0010348.
FBtr0112858; FBpp0111771; FBgn0010348.
FBtr0299906; FBpp0289184; FBgn0010348.
FBtr0299907; FBpp0289185; FBgn0010348.
FBtr0332054; FBpp0304364; FBgn0010348.
GeneIDi40506.
KEGGidme:Dmel_CG8385.
UCSCiCG8385-RB. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiARF1_DROME
AccessioniPrimary (citable) accession number: P61209
Secondary accession number(s): A4V2B1
, A8JNX4, P35676, Q9VNQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families