Reviewed, UniProtKB/Swiss-Prot P61204 (ARF3_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor 3

Gene names

Name:

ARF3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Subunit structure	Interacts with PRKCABP. Interacts with PI4KB and FREQ/NCS1 at the Golgi complex. Ref.8 Ref.9
Subcellular location	Golgi apparatus. Cytoplasm › perinuclear region.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Traceable author statement. Source: ProtInc GTPase activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 181	180	ADP-ribosylation factor 3		PRO_0000207386
Regions
	Nucleotide binding	24 – 31	8	GTP By similarity
	Nucleotide binding	67 – 71	5	GTP By similarity
	Nucleotide binding	126 – 129	4	GTP By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential

Sequences

	Sequence		Length	Mass (Da)
	P61204-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D6FA234DFAED3E5F	FASTA	181	20,601
	10 20 30 40 50 60 MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN 70 80 90 100 110 120 ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV 130 140 150 160 170 180 LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, characterization, and expression of human ADP-ribosylation factors: two guanine nucleotide-dependent activators of cholera toxin." Bobak D.A., Nightingale M.S., Murtagh J.J. Jr., Price S.R., Moss J., Vaughan M. Proc. Natl. Acad. Sci. U.S.A. 86:6101-6105(1989) [PubMed: 2474826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cerebellum.
[2]	"Isolation and characterization of the human gene for ADP-ribosylation factor 3, a 20-kDa guanine nucleotide-binding protein activator of cholera toxin." Tsai S.C., Haun R.S., Tsuchiya M., Moss J., Vaughan M. J. Biol. Chem. 266:23053-23059(1991) [PubMed: 1744102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lung and Skin.
[8]	"Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors." Takeya R., Takeshige K., Sumimoto H. Biochem. Biophys. Res. Commun. 267:149-155(2000) [PubMed: 10623590] [Abstract] Cited for: INTERACTION WITH PRKCABP.
[9]	"Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta." Haynes L.P., Sherwood M.W., Dolman N.J., Burgoyne R.D. Traffic 8:1080-1092(2007) [PubMed: 17555535] [Abstract] Cited for: INTERACTION WITH PI4KB AND FREQ, SUBCELLULAR LOCATION.
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74493 Genomic DNA. Translation: AAA58359.1. M33384 mRNA. Translation: AAA83931.1. M74491 mRNA. Translation: AAB59425.1. AF493882 mRNA. Translation: AAM12596.1. BT006670 mRNA. Translation: AAP35316.1. AK291633 mRNA. Translation: BAF84322.1. CH471111 Genomic DNA. Translation: EAW58026.1. BC007647 mRNA. Translation: AAH07647.1. BC007762 mRNA. Translation: AAH07762.1. BC017565 mRNA. Translation: AAH17565.1. BC028402 mRNA. Translation: AAH28402.1.
IPI	IPI00215917.
PIR	A41570.
RefSeq	NP_001650.1.
UniGene	Hs.119177
3D structure databases
SMR	P61204. Positions 2-180.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61204. 3 interactions.
STRING	P61204.
2-D gel databases
OGP	P16587.
Proteomic databases
PeptideAtlas	P61204.
PRIDE	P61204.
Genome annotation databases
Ensembl	ENST00000256682; ENSP00000256682; ENSG00000134287; Homo sapiens. [Genome view]
GeneID	377.
KEGG	hsa:377.
UCSC	uc001rsr.2. human.
Organism-specific databases
CTD	377.
GeneCards	GC12M047616.
H-InvDB	HIX0010590.
HGNC	HGNC:654. ARF3.
MIM	103190. gene.
PharmGKB	PA24936.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P61204.
HOVERGEN	P61204.
OMA	NAMAIND
Gene expression databases
ArrayExpress	P61204.
Bgee	P61204.
CleanEx	HS_ARF3.
Genevestigator	P61204.
GermOnline	ENSG00000134287. Homo sapiens.
Family and domain databases
InterPro	IPR006688. ARF. IPR006689. ARF/SAR. IPR005225. Small_GTP_bd. [Graphical view]
PANTHER	PTHR11711. ARF/SAR. 1 hit.
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00328. SAR1GTPBP.
SMART	SM00177. ARF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	1579.
SOURCE	Search...

Entry information

Entry name

ARF3_HUMAN

Accession

Primary (citable) accession number: P61204
Secondary accession number(s): A8K6G8, P16587

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.