ARF3

Functionⁱ

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	24 – 31	GTPBy similarity	8
Nucleotide bindingⁱ	67 – 71	GTPBy similarity	5
Nucleotide bindingⁱ	126 – 129	GTPBy similarity	4

GO - Molecular functionⁱ

GTPase activity
Source: ProtInc
Traceable author statementⁱ
- 1744102
GTP binding
Source: ProtInc
Traceable author statementⁱ
- 8947846

Complete GO annotation...

GO - Biological processⁱ

phosphatidylinositol biosynthetic process Source: Reactome
protein transport Source: UniProtKB-KW
retrograde vesicle-mediated transport, Golgi to ER
Source: WormBase
Inferred from genetic interactionⁱ
- 24768165
small GTPase mediated signal transduction Source: InterPro

Complete GO annotation...

Keywordsⁱ

Biological process	ER-Golgi transport, Protein transport, Transport
Ligand	GTP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1660514. Synthesis of PIPs at the Golgi membrane. R-HSA-6807878. COPI-mediated anterograde transport. R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Reactomeⁱ

R-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ADP-ribosylation factor 3
Gene namesⁱ	Name:ARF3
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 12

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:654. ARF3.

HGNCⁱ

HGNC:654. ARF3.

Subcellular locationⁱ

GO - Cellular componentⁱ

extracellular exosome
Source: UniProtKB
Inferred from direct assayⁱ
Golgi membrane Source: Reactome
perinuclear region of cytoplasm Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Golgi apparatus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	377.
Open Targets More...OpenTargetsⁱ	ENSG00000134287.
PharmGKBⁱ	PA24936.

Polymorphism and mutation databases

BioMutaⁱ	ARF3.
DMDMⁱ	47117657.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedSequence analysis
ChainⁱPRO_0000207386	2 – 181	ADP-ribosylation factor 3Add BLAST		180

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Lipidationⁱ	2	N-myristoyl glycineSequence analysis		1

Keywords - PTMⁱ

Lipoprotein, Myristate

Proteomic databases

EPDⁱ	P61204.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P61204.
PaxDbⁱ	P61204.
PeptideAtlas More...PeptideAtlasⁱ	P61204.
PRIDEⁱ	P61204.
TopDownProteomicsⁱ	P61204-1. [P61204-1]

2D gel databases

USC-OGP 2-DE database More...OGPⁱ	P16587.

OGPⁱ

P16587.

PTM databases

iPTMnetⁱ	P61204.
PhosphoSitePlusⁱ	P61204.
SwissPalmⁱ	P61204.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000134287.
CleanExⁱ	HS_ARF3.
ExpressionAtlasⁱ	P61204. baseline and differential.
Genevisibleⁱ	P61204. HS.

Interactionⁱ

Subunit structureⁱ

Interacts with PRKCABP. Interacts with PI4KB and NCS1/FREQ at the Golgi complex.2 Publications

Protein-protein interaction databases

BioGridⁱ	106872. 22 interactors.
IntActⁱ	P61204. 9 interactors.
STRINGⁱ	9606.ENSP00000256682.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P61204.
SMRⁱ	P61204.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0070. Eukaryota. COG1100. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00780000121855.
HOGENOMⁱ	HOG000163691.
HOVERGENⁱ	HBG002073.
InParanoidⁱ	P61204.
KEGG Orthology (KO) More...KOⁱ	K07938.
OMAⁱ	AMREMAN.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0S7S.
PhylomeDBⁱ	P61204.
TreeFamⁱ	TF300808.

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00025. Arf. 1 hit.
PRINTSⁱ	PR00328. SAR1GTPBP.
SUPFAMⁱ	SSF52540. SSF52540. 1 hit.
TIGRFAMsⁱ	TIGR00231. small_GTP. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51417. ARF. 1 hit.

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: P61204-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG 
        60         70         80         90        100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV 
       110        120        130        140        150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH 
       160        170        180 
RNWYIQATCA TSGDGLYEGL DWLANQLKNK K

Length:181

Mass (Da):20,601

Last modified:January 23, 2007 - v2

Checksum:ⁱD6FA234DFAED3E5F

GO

Isoform 2 (identifier: P61204-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
50-86: Missing.

« Hide

        10         20         30         40         50
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG 
        60         70         80         90        100
LIFVVDSNDR ERVNEAREEL MRMLAEDELR DAVLLVFANK QDLPNAMNAA 
       110        120        130        140 
EITDKLGLHS LRHRNWYIQA TCATSGDGLY EGLDWLANQL KNKK

Note: No experimental confirmation available.

Show »

Length:144

Mass (Da):16,145

Checksum:ⁱB66A3E6F9B9225B1

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_056935	50 – 86	Missing in isoform 2. 1 PublicationAdd BLAST		37

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M74493 Genomic DNA. Translation: AAA58359.1. M33384 mRNA. Translation: AAA83931.1. M74491 mRNA. Translation: AAB59425.1. AF493882 mRNA. Translation: AAM12596.1. BT006670 mRNA. Translation: AAP35316.1. AK291633 mRNA. Translation: BAF84322.1. AK316528 mRNA. Translation: BAH14899.1. AC073610 Genomic DNA. No translation available. CH471111 Genomic DNA. Translation: EAW58026.1. BC007647 mRNA. Translation: AAH07647.1. BC007762 mRNA. Translation: AAH07762.1. BC017565 mRNA. Translation: AAH17565.1. BC028402 mRNA. Translation: AAH28402.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS8774.1. [P61204-1]
PIRⁱ	A41570.
NCBI Reference Sequences More...RefSeqⁱ	NP_001650.1. NM_001659.2. [P61204-1] XP_005268913.1. XM_005268856.1. [P61204-1]
UniGeneⁱ	Hs.119177.

Genome annotation databases

Ensemblⁱ	ENST00000256682; ENSP00000256682; ENSG00000134287. [P61204-1] ENST00000447318; ENSP00000395370; ENSG00000134287. [P61204-2] ENST00000541959; ENSP00000438510; ENSG00000134287. [P61204-1]
GeneIDⁱ	377.
KEGGⁱ	hsa:377.
UCSC genome browser More...UCSCⁱ	uc001rsr.3. human. [P61204-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M74493 Genomic DNA. Translation: AAA58359.1. M33384 mRNA. Translation: AAA83931.1. M74491 mRNA. Translation: AAB59425.1. AF493882 mRNA. Translation: AAM12596.1. BT006670 mRNA. Translation: AAP35316.1. AK291633 mRNA. Translation: BAF84322.1. AK316528 mRNA. Translation: BAH14899.1. AC073610 Genomic DNA. No translation available. CH471111 Genomic DNA. Translation: EAW58026.1. BC007647 mRNA. Translation: AAH07647.1. BC007762 mRNA. Translation: AAH07762.1. BC017565 mRNA. Translation: AAH17565.1. BC028402 mRNA. Translation: AAH28402.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS8774.1. [P61204-1]
PIRⁱ	A41570.
NCBI Reference Sequences More...RefSeqⁱ	NP_001650.1. NM_001659.2. [P61204-1] XP_005268913.1. XM_005268856.1. [P61204-1]
UniGeneⁱ	Hs.119177.

3D structure databases

ProteinModelPortalⁱ	P61204.
SMRⁱ	P61204.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	106872. 22 interactors.
IntActⁱ	P61204. 9 interactors.
STRINGⁱ	9606.ENSP00000256682.

PTM databases

iPTMnetⁱ	P61204.
PhosphoSitePlusⁱ	P61204.
SwissPalmⁱ	P61204.

Polymorphism and mutation databases

BioMutaⁱ	ARF3.
DMDMⁱ	47117657.

2D gel databases

USC-OGP 2-DE database More...OGPⁱ	P16587.

OGPⁱ

P16587.

Proteomic databases

EPDⁱ	P61204.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P61204.
PaxDbⁱ	P61204.
PeptideAtlas More...PeptideAtlasⁱ	P61204.
PRIDEⁱ	P61204.
TopDownProteomicsⁱ	P61204-1. [P61204-1]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	377.
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000256682; ENSP00000256682; ENSG00000134287. [P61204-1] ENST00000447318; ENSP00000395370; ENSG00000134287. [P61204-2] ENST00000541959; ENSP00000438510; ENSG00000134287. [P61204-1]
GeneIDⁱ	377.
KEGGⁱ	hsa:377.
UCSC genome browser More...UCSCⁱ	uc001rsr.3. human. [P61204-1]

Organism-specific databases

CTDⁱ	377.
DisGeNET More...DisGeNETⁱ	377.
GeneCardsⁱ	ARF3.
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:654. ARF3.
MIMⁱ	103190. gene.
neXtProtⁱ	NX_P61204.
Open Targets More...OpenTargetsⁱ	ENSG00000134287.
PharmGKBⁱ	PA24936.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0070. Eukaryota. COG1100. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00780000121855.
HOGENOMⁱ	HOG000163691.
HOVERGENⁱ	HBG002073.
InParanoidⁱ	P61204.
KEGG Orthology (KO) More...KOⁱ	K07938.
OMAⁱ	AMREMAN.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0S7S.
PhylomeDBⁱ	P61204.
TreeFamⁱ	TF300808.

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1660514. Synthesis of PIPs at the Golgi membrane. R-HSA-6807878. COPI-mediated anterograde transport. R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Reactomeⁱ

R-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSⁱ	ARF3. human.
GeneWikiⁱ	ARF3.
GenomeRNAiⁱ	377.
Protein Ontology More...PROⁱ	PR:P61204.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000134287.
CleanExⁱ	HS_ARF3.
ExpressionAtlasⁱ	P61204. baseline and differential.
Genevisibleⁱ	P61204. HS.

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00025. Arf. 1 hit.
PRINTSⁱ	PR00328. SAR1GTPBP.
SUPFAMⁱ	SSF52540. SSF52540. 1 hit.
TIGRFAMsⁱ	TIGR00231. small_GTP. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51417. ARF. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ARF3_HUMAN
Accessionⁱ	P61204Primary (citable) accession number: P61204 Secondary accession number(s): A8K6G8, B7ZB63, P16587
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
	Last sequence update:	January 23, 2007
	Last modified:	June 7, 2017
	This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P61204 (ARF3_HUMAN)

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ADP-ribosylation factor 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequences (2)ⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ