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Protein

ADP-ribosylation factor 3

Gene

ARF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: ProtInc
  • GTP binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 3
Gene namesi
Name:ARF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:654. ARF3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24936.

Polymorphism and mutation databases

BioMutaiARF3.
DMDMi47117657.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 181180ADP-ribosylation factor 3PRO_0000207386Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP61204.
MaxQBiP61204.
PaxDbiP61204.
PeptideAtlasiP61204.
PRIDEiP61204.
TopDownProteomicsiP61204-1. [P61204-1]

2D gel databases

OGPiP16587.

PTM databases

iPTMnetiP61204.
PhosphoSiteiP61204.
SwissPalmiP61204.

Expressioni

Gene expression databases

BgeeiENSG00000134287.
CleanExiHS_ARF3.
ExpressionAtlasiP61204. baseline and differential.
GenevisibleiP61204. HS.

Organism-specific databases

HPAiCAB015196.

Interactioni

Subunit structurei

Interacts with PRKCABP. Interacts with PI4KB and NCS1/FREQ at the Golgi complex.2 Publications

Protein-protein interaction databases

BioGridi106872. 21 interactions.
IntActiP61204. 7 interactions.
STRINGi9606.ENSP00000256682.

Structurei

3D structure databases

ProteinModelPortaliP61204.
SMRiP61204. Positions 18-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61204.
KOiK07938.
OMAiIQTTCAT.
OrthoDBiEOG091G0S7S.
PhylomeDBiP61204.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61204-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLANQLKNK K
Length:181
Mass (Da):20,601
Last modified:January 23, 2007 - v2
Checksum:iD6FA234DFAED3E5F
GO
Isoform 2 (identifier: P61204-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-86: Missing.

Note: No experimental confirmation available.
Show »
Length:144
Mass (Da):16,145
Checksum:iB66A3E6F9B9225B1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei50 – 8637Missing in isoform 2. 1 PublicationVSP_056935Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74493 Genomic DNA. Translation: AAA58359.1.
M33384 mRNA. Translation: AAA83931.1.
M74491 mRNA. Translation: AAB59425.1.
AF493882 mRNA. Translation: AAM12596.1.
BT006670 mRNA. Translation: AAP35316.1.
AK291633 mRNA. Translation: BAF84322.1.
AK316528 mRNA. Translation: BAH14899.1.
AC073610 Genomic DNA. No translation available.
CH471111 Genomic DNA. Translation: EAW58026.1.
BC007647 mRNA. Translation: AAH07647.1.
BC007762 mRNA. Translation: AAH07762.1.
BC017565 mRNA. Translation: AAH17565.1.
BC028402 mRNA. Translation: AAH28402.1.
CCDSiCCDS8774.1. [P61204-1]
PIRiA41570.
RefSeqiNP_001650.1. NM_001659.2. [P61204-1]
XP_005268913.1. XM_005268856.1. [P61204-1]
UniGeneiHs.119177.

Genome annotation databases

EnsembliENST00000256682; ENSP00000256682; ENSG00000134287. [P61204-1]
ENST00000447318; ENSP00000395370; ENSG00000134287. [P61204-2]
ENST00000541959; ENSP00000438510; ENSG00000134287. [P61204-1]
GeneIDi377.
KEGGihsa:377.
UCSCiuc001rsr.3. human. [P61204-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74493 Genomic DNA. Translation: AAA58359.1.
M33384 mRNA. Translation: AAA83931.1.
M74491 mRNA. Translation: AAB59425.1.
AF493882 mRNA. Translation: AAM12596.1.
BT006670 mRNA. Translation: AAP35316.1.
AK291633 mRNA. Translation: BAF84322.1.
AK316528 mRNA. Translation: BAH14899.1.
AC073610 Genomic DNA. No translation available.
CH471111 Genomic DNA. Translation: EAW58026.1.
BC007647 mRNA. Translation: AAH07647.1.
BC007762 mRNA. Translation: AAH07762.1.
BC017565 mRNA. Translation: AAH17565.1.
BC028402 mRNA. Translation: AAH28402.1.
CCDSiCCDS8774.1. [P61204-1]
PIRiA41570.
RefSeqiNP_001650.1. NM_001659.2. [P61204-1]
XP_005268913.1. XM_005268856.1. [P61204-1]
UniGeneiHs.119177.

3D structure databases

ProteinModelPortaliP61204.
SMRiP61204. Positions 18-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106872. 21 interactions.
IntActiP61204. 7 interactions.
STRINGi9606.ENSP00000256682.

PTM databases

iPTMnetiP61204.
PhosphoSiteiP61204.
SwissPalmiP61204.

Polymorphism and mutation databases

BioMutaiARF3.
DMDMi47117657.

2D gel databases

OGPiP16587.

Proteomic databases

EPDiP61204.
MaxQBiP61204.
PaxDbiP61204.
PeptideAtlasiP61204.
PRIDEiP61204.
TopDownProteomicsiP61204-1. [P61204-1]

Protocols and materials databases

DNASUi377.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256682; ENSP00000256682; ENSG00000134287. [P61204-1]
ENST00000447318; ENSP00000395370; ENSG00000134287. [P61204-2]
ENST00000541959; ENSP00000438510; ENSG00000134287. [P61204-1]
GeneIDi377.
KEGGihsa:377.
UCSCiuc001rsr.3. human. [P61204-1]

Organism-specific databases

CTDi377.
GeneCardsiARF3.
HGNCiHGNC:654. ARF3.
HPAiCAB015196.
MIMi103190. gene.
neXtProtiNX_P61204.
PharmGKBiPA24936.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP61204.
KOiK07938.
OMAiIQTTCAT.
OrthoDBiEOG091G0S7S.
PhylomeDBiP61204.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiARF3. human.
GeneWikiiARF3.
GenomeRNAii377.
PROiP61204.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134287.
CleanExiHS_ARF3.
ExpressionAtlasiP61204. baseline and differential.
GenevisibleiP61204. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF3_HUMAN
AccessioniPrimary (citable) accession number: P61204
Secondary accession number(s): A8K6G8, B7ZB63, P16587
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.