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Protein

ADP-ribosylation factor 3

Gene

ARF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: ProtInc
  • GTP binding Source: ProtInc

GO - Biological processi

  • phosphatidylinositol biosynthetic process Source: Reactome
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: WormBase

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660514 Synthesis of PIPs at the Golgi membrane
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 3
Gene namesi
Name:ARF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000134287.9
HGNCiHGNC:654 ARF3
MIMi103190 gene
neXtProtiNX_P61204

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi377
OpenTargetsiENSG00000134287
PharmGKBiPA24936

Polymorphism and mutation databases

BioMutaiARF3
DMDMi47117657

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002073862 – 181ADP-ribosylation factor 3Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP61204
MaxQBiP61204
PaxDbiP61204
PeptideAtlasiP61204
PRIDEiP61204
TopDownProteomicsiP61204
P61204-1 [P61204-1]

2D gel databases

OGPiP16587

PTM databases

iPTMnetiP61204
PhosphoSitePlusiP61204
SwissPalmiP61204

Expressioni

Gene expression databases

BgeeiENSG00000134287
CleanExiHS_ARF3
ExpressionAtlasiP61204 baseline and differential
GenevisibleiP61204 HS

Interactioni

Subunit structurei

Interacts with PRKCABP. Interacts with PI4KB and NCS1/FREQ at the Golgi complex.2 Publications

Protein-protein interaction databases

BioGridi106872, 25 interactors
IntActiP61204, 9 interactors
STRINGi9606.ENSP00000256682

Structurei

3D structure databases

ProteinModelPortaliP61204
SMRiP61204
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP61204
KOiK07938
OMAiAMREMAN
OrthoDBiEOG091G0S7S
PhylomeDBiP61204
TreeFamiTF300808

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61204-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLANQLKNK K
Length:181
Mass (Da):20,601
Last modified:January 23, 2007 - v2
Checksum:iD6FA234DFAED3E5F
GO
Isoform 2 (identifier: P61204-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-86: Missing.

Note: No experimental confirmation available.
Show »
Length:144
Mass (Da):16,145
Checksum:iB66A3E6F9B9225B1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05693550 – 86Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74493 Genomic DNA Translation: AAA58359.1
M33384 mRNA Translation: AAA83931.1
M74491 mRNA Translation: AAB59425.1
AF493882 mRNA Translation: AAM12596.1
BT006670 mRNA Translation: AAP35316.1
AK291633 mRNA Translation: BAF84322.1
AK316528 mRNA Translation: BAH14899.1
AC073610 Genomic DNA No translation available.
CH471111 Genomic DNA Translation: EAW58026.1
BC007647 mRNA Translation: AAH07647.1
BC007762 mRNA Translation: AAH07762.1
BC017565 mRNA Translation: AAH17565.1
BC028402 mRNA Translation: AAH28402.1
CCDSiCCDS8774.1 [P61204-1]
PIRiA41570
RefSeqiNP_001650.1, NM_001659.2 [P61204-1]
XP_005268913.1, XM_005268856.1 [P61204-1]
UniGeneiHs.119177

Genome annotation databases

EnsembliENST00000256682; ENSP00000256682; ENSG00000134287 [P61204-1]
ENST00000447318; ENSP00000395370; ENSG00000134287 [P61204-2]
ENST00000541959; ENSP00000438510; ENSG00000134287 [P61204-1]
GeneIDi377
KEGGihsa:377
UCSCiuc001rsr.3 human [P61204-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARF3_HUMAN
AccessioniPrimary (citable) accession number: P61204
Secondary accession number(s): A8K6G8, B7ZB63, P16587
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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