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P61201

- CSN2_HUMAN

UniProt

P61201 - CSN2_HUMAN

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Protein

COP9 signalosome complex subunit 2

Gene

COPS2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.5 Publications

GO - Molecular functioni

  1. signal transducer activity Source: UniProtKB
  2. transcription corepressor activity Source: Ensembl

GO - Biological processi

  1. cell proliferation Source: Ensembl
  2. cullin deneddylation Source: UniProtKB
  3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  4. neuron differentiation Source: Ensembl
  5. signal transduction Source: UniProtKB
  6. skeletal muscle cell differentiation Source: Ensembl
  7. transcription from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

SignaLinkiP61201.

Names & Taxonomyi

Protein namesi
Recommended name:
COP9 signalosome complex subunit 2
Short name:
SGN2
Short name:
Signalosome subunit 2
Alternative name(s):
Alien homolog
JAB1-containing signalosome subunit 2
Thyroid receptor-interacting protein 15
Short name:
TR-interacting protein 15
Short name:
TRIP-15
Gene namesi
Name:COPS2
Synonyms:CSN2, TRIP15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:30747. COPS2.

Subcellular locationi

GO - Cellular componenti

  1. COP9 signalosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signalosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134952445.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443COP9 signalosome complex subunit 2PRO_0000120968Add
BLAST

Post-translational modificationi

Phosphorylated by CK2 and PKD kinases.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP61201.
PaxDbiP61201.
PRIDEiP61201.

PTM databases

PhosphoSiteiP61201.

Expressioni

Gene expression databases

BgeeiP61201.
CleanExiHS_COPS2.
HS_CSN2.
ExpressionAtlasiP61201. baseline and differential.
GenevestigatoriP61201.

Organism-specific databases

HPAiHPA016867.
HPA018271.

Interactioni

Subunit structurei

Interacts with NIF3L1 (By similarity). Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS4, COPS5 COPS6 and COPS7 (COPS7A or COPS7B). Interacts with CUL1 and CUL2. Specifically interacts with the ligand binding domain of the thyroid receptor (TR). Does not require the presence of thyroid hormone for its interaction. Interacts with IRF8/ICSBP1 and with nuclear receptors NR2F1 and NR0B1.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P299913EBI-1050386,EBI-8826488From a different organism.
CREBBPQ927933EBI-1050386,EBI-81215
CrebbpP454812EBI-1050386,EBI-296306From a different organism.
EP300Q094722EBI-1050386,EBI-447295

Protein-protein interaction databases

BioGridi114729. 108 interactions.
DIPiDIP-42076N.
IntActiP61201. 18 interactions.
MINTiMINT-1204035.
STRINGi9606.ENSP00000299259.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4D10X-ray3.80B/J1-443[»]
4D18X-ray4.08B/J1-443[»]
ProteinModelPortaliP61201.
SMRiP61201. Positions 30-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini248 – 413166PCIAdd
BLAST

Sequence similaritiesi

Belongs to the CSN2 family.Curated
Contains 1 PCI domain.Curated

Phylogenomic databases

eggNOGiCOG5159.
GeneTreeiENSGT00530000063301.
HOVERGENiHBG003924.
InParanoidiP61201.
KOiK12176.
OMAiYGLEYSE.
PhylomeDBiP61201.
TreeFamiTF105738.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR013143. PAM.
IPR000717. PCI_dom.
IPR011990. TPR-like_helical_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00753. PAM. 1 hit.
SM00088. PINT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P61201-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDMEDDFMC DDEEDYDLEY SEDSNSEPNV DLENQYYNSK ALKEDDPKAA
60 70 80 90 100
LSSFQKVLEL EGEKGEWGFK ALKQMIKINF KLTNFPEMMN RYKQLLTYIR
110 120 130 140 150
SAVTRNYSEK SINSILDYIS TSKQMDLLQE FYETTLEALK DAKNDRLWFK
160 170 180 190 200
TNTKLGKLYL EREEYGKLQK ILRQLHQSCQ TDDGEDDLKK GTQLLEIYAL
210 220 230 240 250
EIQMYTAQKN NKKLKALYEQ SLHIKSAIPH PLIMGVIREC GGKMHLREGE
260 270 280 290 300
FEKAHTDFFE AFKNYDESGS PRRTTCLKYL VLANMLMKSG INPFDSQEAK
310 320 330 340 350
PYKNDPEILA MTNLVSAYQN NDITEFEKIL KTNHSNIMDD PFIREHIEEL
360 370 380 390 400
LRNIRTQVLI KLIKPYTRIH IPFISKELNI DVADVESLLV QCILDNTIHG
410 420 430 440
RIDQVNQLLE LDHQKRGGAR YTALDKWTNQ LNSLNQAVVS KLA
Length:443
Mass (Da):51,597
Last modified:May 10, 2004 - v1
Checksum:i1DB6FA774C13BC59
GO
Isoform 2 (identifier: P61201-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-124: Q → QNSDFLCQ

Show »
Length:450
Mass (Da):52,405
Checksum:i581DE764677E90C0
GO

Sequence cautioni

The sequence AAD30269.1 differs from that shown. Reason: Frameshift at position 304. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361Y → N in CAG46860. 1 PublicationCurated
Sequence conflicti151 – 1511T → A in AAD43026. (PubMed:10931946)Curated
Sequence conflicti211 – 2111N → T in CAG46860. 1 PublicationCurated
Sequence conflicti352 – 3521R → Q(PubMed:10207062)Curated
Sequence conflicti392 – 3921C → S(PubMed:10207062)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei124 – 1241Q → QNSDFLCQ in isoform 2. 1 PublicationVSP_011884

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF084260 mRNA. Translation: AAC34122.1.
AF100762 mRNA. Translation: AAD43026.1.
AF212227 mRNA. Translation: AAK26250.1.
CR542063 mRNA. Translation: CAG46860.1.
BC012629 mRNA. Translation: AAH12629.1.
L40388 mRNA. Translation: AAC41734.1.
AF120268 mRNA. Translation: AAD30269.1. Frameshift.
CCDSiCCDS32235.1. [P61201-1]
CCDS45257.1. [P61201-2]
RefSeqiNP_001137359.1. NM_001143887.1. [P61201-2]
NP_004227.1. NM_004236.3. [P61201-1]
UniGeneiHs.369614.

Genome annotation databases

EnsembliENST00000299259; ENSP00000299259; ENSG00000166200. [P61201-2]
ENST00000388901; ENSP00000373553; ENSG00000166200. [P61201-1]
GeneIDi9318.
KEGGihsa:9318.
UCSCiuc001zxf.3. human. [P61201-1]
uc001zxh.3. human. [P61201-2]

Polymorphism databases

DMDMi47117681.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF084260 mRNA. Translation: AAC34122.1 .
AF100762 mRNA. Translation: AAD43026.1 .
AF212227 mRNA. Translation: AAK26250.1 .
CR542063 mRNA. Translation: CAG46860.1 .
BC012629 mRNA. Translation: AAH12629.1 .
L40388 mRNA. Translation: AAC41734.1 .
AF120268 mRNA. Translation: AAD30269.1 . Frameshift.
CCDSi CCDS32235.1. [P61201-1 ]
CCDS45257.1. [P61201-2 ]
RefSeqi NP_001137359.1. NM_001143887.1. [P61201-2 ]
NP_004227.1. NM_004236.3. [P61201-1 ]
UniGenei Hs.369614.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4D10 X-ray 3.80 B/J 1-443 [» ]
4D18 X-ray 4.08 B/J 1-443 [» ]
ProteinModelPortali P61201.
SMRi P61201. Positions 30-443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114729. 108 interactions.
DIPi DIP-42076N.
IntActi P61201. 18 interactions.
MINTi MINT-1204035.
STRINGi 9606.ENSP00000299259.

PTM databases

PhosphoSitei P61201.

Polymorphism databases

DMDMi 47117681.

Proteomic databases

MaxQBi P61201.
PaxDbi P61201.
PRIDEi P61201.

Protocols and materials databases

DNASUi 9318.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000299259 ; ENSP00000299259 ; ENSG00000166200 . [P61201-2 ]
ENST00000388901 ; ENSP00000373553 ; ENSG00000166200 . [P61201-1 ]
GeneIDi 9318.
KEGGi hsa:9318.
UCSCi uc001zxf.3. human. [P61201-1 ]
uc001zxh.3. human. [P61201-2 ]

Organism-specific databases

CTDi 9318.
GeneCardsi GC15M049417.
HGNCi HGNC:30747. COPS2.
HPAi HPA016867.
HPA018271.
MIMi 604508. gene.
neXtProti NX_P61201.
PharmGKBi PA134952445.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5159.
GeneTreei ENSGT00530000063301.
HOVERGENi HBG003924.
InParanoidi P61201.
KOi K12176.
OMAi YGLEYSE.
PhylomeDBi P61201.
TreeFami TF105738.

Enzyme and pathway databases

SignaLinki P61201.

Miscellaneous databases

ChiTaRSi COPS2. human.
GeneWikii COPS2.
GenomeRNAii 9318.
NextBioi 34905.
PROi P61201.
SOURCEi Search...

Gene expression databases

Bgeei P61201.
CleanExi HS_COPS2.
HS_CSN2.
ExpressionAtlasi P61201. baseline and differential.
Genevestigatori P61201.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.25.40.10. 1 hit.
InterProi IPR013143. PAM.
IPR000717. PCI_dom.
IPR011990. TPR-like_helical_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01399. PCI. 1 hit.
[Graphical view ]
SMARTi SM00753. PAM. 1 hit.
SM00088. PINT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Dumdey R., Bech-Otschir D., Ferrell K., Dubiel W.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pituitary.
  3. Peng Y., Li Y., Tu Y., Xu S., Han Z., Fu G., Chen Z.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Pituitary.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  6. "Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor."
    Lee J.W., Choi H.-S., Gyuris J., Brent R., Moore D.D.
    Mol. Endocrinol. 9:243-254(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-236 (ISOFORM 1).
  7. "Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily."
    Dressel U., Thormeyer D., Altincicek B., Paululat A., Eggert M., Schneider S., Tenbaum S.P., Renkawitz R., Baniahmad A.
    Mol. Cell. Biol. 19:3383-3394(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-443 (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH NR2F1.
  8. "A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits."
    Seeger M., Kraft R., Ferrell K., Bech-Otschir D., Dumdey R., Schade R., Gordon C., Naumann M., Dubiel W.
    FASEB J. 12:469-478(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
  9. "Interaction of the corepressor Alien with DAX-1 is abrogated by mutations of DAX-1 involved in adrenal hypoplasia congenita."
    Altincicek B., Tenbaum S.P., Dressel U., Thormeyer D., Renkawitz R., Baniahmad A.
    J. Biol. Chem. 275:7662-7667(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NR0B1.
  10. "Interaction between interferon consensus sequence-binding protein and COP9/signalosome subunit CSN2 (Trip15). A possible link between interferon regulatory factor signaling and the COP9/signalosome."
    Cohen H., Azriel A., Cohen T., Meraro D., Hashmueli S., Bech-Otschir D., Kraft R., Dubiel W., Levi B.Z.
    J. Biol. Chem. 275:39081-39089(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IRF8/ICSBP1.
  11. "COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system."
    Bech-Otschir D., Kraft R., Huang X., Henklein P., Kapelari B., Pollmann C., Dubiel W.
    EMBO J. 20:1630-1639(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: FUNCTION, COMPOSITION OF THE CSN COMPLEX, INTERACTION WITH CUL1.
  13. "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage."
    Groisman R., Polanowska J., Kuraoka I., Sawada J., Saijo M., Drapkin R., Kisselev A.F., Tanaka K., Nakatani Y.
    Cell 113:357-367(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome."
    Uhle S., Medalia O., Waldron R., Dumdey R., Henklein P., Bech-Otschir D., Huang X., Berse M., Sperling J., Schade R., Dubiel W.
    EMBO J. 22:1302-1312(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  15. "Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry."
    Fang L., Wang X., Yamoah K., Chen P.L., Pan Z.Q., Huang L.
    J. Proteome Res. 7:4914-4925(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CSN COMPLEX.
  16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCSN2_HUMAN
AccessioniPrimary (citable) accession number: P61201
Secondary accession number(s): O88950
, Q15647, Q6FGP4, Q9BY54, Q9R249, Q9UNI2, Q9UNQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3