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P61201

- CSN2_HUMAN

UniProt

P61201 - CSN2_HUMAN

Protein

COP9 signalosome complex subunit 2

Gene

COPS2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1.5 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. signal transducer activity Source: UniProtKB
    3. transcription corepressor activity Source: Ensembl

    GO - Biological processi

    1. cell proliferation Source: Ensembl
    2. cullin deneddylation Source: UniProtKB
    3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    4. neuron differentiation Source: Ensembl
    5. signal transduction Source: UniProtKB
    6. skeletal muscle cell differentiation Source: Ensembl
    7. transcription from RNA polymerase II promoter Source: UniProtKB

    Enzyme and pathway databases

    SignaLinkiP61201.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    COP9 signalosome complex subunit 2
    Short name:
    SGN2
    Short name:
    Signalosome subunit 2
    Alternative name(s):
    Alien homolog
    JAB1-containing signalosome subunit 2
    Thyroid receptor-interacting protein 15
    Short name:
    TR-interacting protein 15
    Short name:
    TRIP-15
    Gene namesi
    Name:COPS2
    Synonyms:CSN2, TRIP15
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:30747. COPS2.

    Subcellular locationi

    GO - Cellular componenti

    1. COP9 signalosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Signalosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134952445.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443COP9 signalosome complex subunit 2PRO_0000120968Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CK2 and PKD kinases.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP61201.
    PaxDbiP61201.
    PRIDEiP61201.

    PTM databases

    PhosphoSiteiP61201.

    Expressioni

    Gene expression databases

    ArrayExpressiP61201.
    BgeeiP61201.
    CleanExiHS_COPS2.
    HS_CSN2.
    GenevestigatoriP61201.

    Organism-specific databases

    HPAiHPA016867.
    HPA018271.

    Interactioni

    Subunit structurei

    Interacts with NIF3L1 By similarity. Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS4, COPS5 COPS6 and COPS7 (COPS7A or COPS7B). Interacts with CUL1 and CUL2. Specifically interacts with the ligand binding domain of the thyroid receptor (TR). Does not require the presence of thyroid hormone for its interaction. Interacts with IRF8/ICSBP1 and with nuclear receptors NR2F1 and NR0B1.By similarity5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P299913EBI-1050386,EBI-8826488From a different organism.
    CREBBPQ927933EBI-1050386,EBI-81215
    CrebbpP454812EBI-1050386,EBI-296306From a different organism.
    EP300Q094722EBI-1050386,EBI-447295

    Protein-protein interaction databases

    BioGridi114729. 92 interactions.
    DIPiDIP-42076N.
    IntActiP61201. 18 interactions.
    MINTiMINT-1204035.
    STRINGi9606.ENSP00000299259.

    Structurei

    3D structure databases

    ProteinModelPortaliP61201.
    SMRiP61201. Positions 71-98, 133-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini248 – 413166PCIAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CSN2 family.Curated
    Contains 1 PCI domain.Curated

    Phylogenomic databases

    eggNOGiCOG5159.
    HOVERGENiHBG003924.
    KOiK12176.
    OMAiYGLEYSE.
    PhylomeDBiP61201.
    TreeFamiTF105738.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.25.40.10. 1 hit.
    InterProiIPR013143. PAM.
    IPR000717. PCI_dom.
    IPR011990. TPR-like_helical.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01399. PCI. 1 hit.
    [Graphical view]
    SMARTiSM00753. PAM. 1 hit.
    SM00088. PINT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P61201-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSDMEDDFMC DDEEDYDLEY SEDSNSEPNV DLENQYYNSK ALKEDDPKAA    50
    LSSFQKVLEL EGEKGEWGFK ALKQMIKINF KLTNFPEMMN RYKQLLTYIR 100
    SAVTRNYSEK SINSILDYIS TSKQMDLLQE FYETTLEALK DAKNDRLWFK 150
    TNTKLGKLYL EREEYGKLQK ILRQLHQSCQ TDDGEDDLKK GTQLLEIYAL 200
    EIQMYTAQKN NKKLKALYEQ SLHIKSAIPH PLIMGVIREC GGKMHLREGE 250
    FEKAHTDFFE AFKNYDESGS PRRTTCLKYL VLANMLMKSG INPFDSQEAK 300
    PYKNDPEILA MTNLVSAYQN NDITEFEKIL KTNHSNIMDD PFIREHIEEL 350
    LRNIRTQVLI KLIKPYTRIH IPFISKELNI DVADVESLLV QCILDNTIHG 400
    RIDQVNQLLE LDHQKRGGAR YTALDKWTNQ LNSLNQAVVS KLA 443
    Length:443
    Mass (Da):51,597
    Last modified:May 10, 2004 - v1
    Checksum:i1DB6FA774C13BC59
    GO
    Isoform 2 (identifier: P61201-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         124-124: Q → QNSDFLCQ

    Show »
    Length:450
    Mass (Da):52,405
    Checksum:i581DE764677E90C0
    GO

    Sequence cautioni

    The sequence AAD30269.1 differs from that shown. Reason: Frameshift at position 304.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361Y → N in CAG46860. 1 PublicationCurated
    Sequence conflicti151 – 1511T → A in AAD43026. (PubMed:10931946)Curated
    Sequence conflicti211 – 2111N → T in CAG46860. 1 PublicationCurated
    Sequence conflicti352 – 3521R → Q(PubMed:10207062)Curated
    Sequence conflicti392 – 3921C → S(PubMed:10207062)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei124 – 1241Q → QNSDFLCQ in isoform 2. 1 PublicationVSP_011884

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084260 mRNA. Translation: AAC34122.1.
    AF100762 mRNA. Translation: AAD43026.1.
    AF212227 mRNA. Translation: AAK26250.1.
    CR542063 mRNA. Translation: CAG46860.1.
    BC012629 mRNA. Translation: AAH12629.1.
    L40388 mRNA. Translation: AAC41734.1.
    AF120268 mRNA. Translation: AAD30269.1. Frameshift.
    CCDSiCCDS32235.1. [P61201-1]
    CCDS45257.1. [P61201-2]
    RefSeqiNP_001137359.1. NM_001143887.1. [P61201-2]
    NP_004227.1. NM_004236.3. [P61201-1]
    UniGeneiHs.369614.

    Genome annotation databases

    EnsembliENST00000299259; ENSP00000299259; ENSG00000166200. [P61201-2]
    ENST00000388901; ENSP00000373553; ENSG00000166200. [P61201-1]
    GeneIDi9318.
    KEGGihsa:9318.
    UCSCiuc001zxf.3. human. [P61201-1]
    uc001zxh.3. human. [P61201-2]

    Polymorphism databases

    DMDMi47117681.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084260 mRNA. Translation: AAC34122.1 .
    AF100762 mRNA. Translation: AAD43026.1 .
    AF212227 mRNA. Translation: AAK26250.1 .
    CR542063 mRNA. Translation: CAG46860.1 .
    BC012629 mRNA. Translation: AAH12629.1 .
    L40388 mRNA. Translation: AAC41734.1 .
    AF120268 mRNA. Translation: AAD30269.1 . Frameshift.
    CCDSi CCDS32235.1. [P61201-1 ]
    CCDS45257.1. [P61201-2 ]
    RefSeqi NP_001137359.1. NM_001143887.1. [P61201-2 ]
    NP_004227.1. NM_004236.3. [P61201-1 ]
    UniGenei Hs.369614.

    3D structure databases

    ProteinModelPortali P61201.
    SMRi P61201. Positions 71-98, 133-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114729. 92 interactions.
    DIPi DIP-42076N.
    IntActi P61201. 18 interactions.
    MINTi MINT-1204035.
    STRINGi 9606.ENSP00000299259.

    PTM databases

    PhosphoSitei P61201.

    Polymorphism databases

    DMDMi 47117681.

    Proteomic databases

    MaxQBi P61201.
    PaxDbi P61201.
    PRIDEi P61201.

    Protocols and materials databases

    DNASUi 9318.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000299259 ; ENSP00000299259 ; ENSG00000166200 . [P61201-2 ]
    ENST00000388901 ; ENSP00000373553 ; ENSG00000166200 . [P61201-1 ]
    GeneIDi 9318.
    KEGGi hsa:9318.
    UCSCi uc001zxf.3. human. [P61201-1 ]
    uc001zxh.3. human. [P61201-2 ]

    Organism-specific databases

    CTDi 9318.
    GeneCardsi GC15M049417.
    HGNCi HGNC:30747. COPS2.
    HPAi HPA016867.
    HPA018271.
    MIMi 604508. gene.
    neXtProti NX_P61201.
    PharmGKBi PA134952445.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5159.
    HOVERGENi HBG003924.
    KOi K12176.
    OMAi YGLEYSE.
    PhylomeDBi P61201.
    TreeFami TF105738.

    Enzyme and pathway databases

    SignaLinki P61201.

    Miscellaneous databases

    GeneWikii COPS2.
    GenomeRNAii 9318.
    NextBioi 34905.
    PROi P61201.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61201.
    Bgeei P61201.
    CleanExi HS_COPS2.
    HS_CSN2.
    Genevestigatori P61201.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.25.40.10. 1 hit.
    InterProi IPR013143. PAM.
    IPR000717. PCI_dom.
    IPR011990. TPR-like_helical.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01399. PCI. 1 hit.
    [Graphical view ]
    SMARTi SM00753. PAM. 1 hit.
    SM00088. PINT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Dumdey R., Bech-Otschir D., Ferrell K., Dubiel W.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Pituitary.
    3. Peng Y., Li Y., Tu Y., Xu S., Han Z., Fu G., Chen Z.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Pituitary.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skeletal muscle.
    6. "Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor."
      Lee J.W., Choi H.-S., Gyuris J., Brent R., Moore D.D.
      Mol. Endocrinol. 9:243-254(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-236 (ISOFORM 1).
    7. "Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily."
      Dressel U., Thormeyer D., Altincicek B., Paululat A., Eggert M., Schneider S., Tenbaum S.P., Renkawitz R., Baniahmad A.
      Mol. Cell. Biol. 19:3383-3394(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-443 (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH NR2F1.
    8. "A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits."
      Seeger M., Kraft R., Ferrell K., Bech-Otschir D., Dumdey R., Schade R., Gordon C., Naumann M., Dubiel W.
      FASEB J. 12:469-478(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
    9. "Interaction of the corepressor Alien with DAX-1 is abrogated by mutations of DAX-1 involved in adrenal hypoplasia congenita."
      Altincicek B., Tenbaum S.P., Dressel U., Thormeyer D., Renkawitz R., Baniahmad A.
      J. Biol. Chem. 275:7662-7667(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NR0B1.
    10. "Interaction between interferon consensus sequence-binding protein and COP9/signalosome subunit CSN2 (Trip15). A possible link between interferon regulatory factor signaling and the COP9/signalosome."
      Cohen H., Azriel A., Cohen T., Meraro D., Hashmueli S., Bech-Otschir D., Kraft R., Dubiel W., Levi B.Z.
      J. Biol. Chem. 275:39081-39089(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IRF8/ICSBP1.
    11. "COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system."
      Bech-Otschir D., Kraft R., Huang X., Henklein P., Kapelari B., Pollmann C., Dubiel W.
      EMBO J. 20:1630-1639(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. Cited for: FUNCTION, COMPOSITION OF THE CSN COMPLEX, INTERACTION WITH CUL1.
    13. "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage."
      Groisman R., Polanowska J., Kuraoka I., Sawada J., Saijo M., Drapkin R., Kisselev A.F., Tanaka K., Nakatani Y.
      Cell 113:357-367(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome."
      Uhle S., Medalia O., Waldron R., Dumdey R., Henklein P., Bech-Otschir D., Huang X., Berse M., Sperling J., Schade R., Dubiel W.
      EMBO J. 22:1302-1312(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    15. "Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry."
      Fang L., Wang X., Yamoah K., Chen P.L., Pan Z.Q., Huang L.
      J. Proteome Res. 7:4914-4925(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CSN COMPLEX.
    16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCSN2_HUMAN
    AccessioniPrimary (citable) accession number: P61201
    Secondary accession number(s): O88950
    , Q15647, Q6FGP4, Q9BY54, Q9R249, Q9UNI2, Q9UNQ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3