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Protein

Lipoyl synthase

Gene

lipA

Organism
Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi70Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi74Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi77Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTDEN243275:G1G0Q-1601-MONOMER
UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:TDE_1575
OrganismiTreponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Taxonomic identifieri243275 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000008212 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023741 – 290Lipoyl synthaseAdd BLAST290

Proteomic databases

PRIDEiP61199

Interactioni

Protein-protein interaction databases

STRINGi243275.TDE1575

Structurei

3D structure databases

ProteinModelPortaliP61199
SMRiP61199
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

P61199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFKAGFMTC NQRKPDWLKI KLPTGELSQE VSNTIKIHKL NTICTSGKCP
60 70 80 90 100
NQGECWRCGT ATFMICGNIC TRACKFCNVP TGCPLPLNPN EPMEIAQSVE
110 120 130 140 150
ALKLKHVVLT SVDRDDIKDF GASHWVKVIR AVKQKTPNVT MEVLIPDFQG
160 170 180 190 200
HEDLVSMIIE AKPEVISHNL ETVRRLSPHV RSRATYDTSL KVLKQIADSG
210 220 230 240 250
LVCKSGIMLG LGETRAEILE TMDDLRKINC KVMTIGQYLR PSIKNIEVKE
260 270 280 290
YVRPEVFEEY KQIGLEKGFS FVESGPLVRS SYHAEKHVLS
Length:290
Mass (Da):32,592
Last modified:May 10, 2004 - v1
Checksum:i5FF6BFA727192040
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017226 Genomic DNA Translation: AAS12092.1
RefSeqiNP_972181.1, NC_002967.9
WP_010957001.1, NC_002967.9

Genome annotation databases

EnsemblBacteriaiAAS12092; AAS12092; TDE_1575
GeneIDi2739325
KEGGitde:TDE1575
PATRICifig|243275.7.peg.1504

Similar proteinsi

Entry informationi

Entry nameiLIPA_TREDE
AccessioniPrimary (citable) accession number: P61199
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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