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P61196

- LIPA_GEOSL

UniProt

P61196 - LIPA_GEOSL

Protein

Lipoyl synthase

Gene

lipA

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (10 May 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi35 – 351Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi61 – 611Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciGSUL243231:GH27-385-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:GSU0380
    OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
    Taxonomic identifieri243231 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
    ProteomesiUP000000577: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Lipoyl synthasePRO_0000102316Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243231.GSU0380.

    Structurei

    3D structure databases

    ProteinModelPortaliP61196.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiITRRPEW.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P61196-1 [UniParc]FASTAAdd to Basket

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    MNIHRKPEWL RKKINPAAHG AMDELLGELR LHTVCREARC PNITECFRER    50
    QATFLILGAE CTRLCSFCNV TKGEPLPPDP DEPARVAQAV VRLSLAHVVI 100
    TSPTRDDLPD GGAGHYVATV ATIGRVAPAT VVELLIPDFL GSRAALADVV 150
    AAAPRIIGHN VETVPRLYAI RAGADYGRSL AVLRTLRELA PGCATKSGLM 200
    LGLGETEEEV LAVMADLRRV DCTYLSLGQY LAPSRFHHPV REFVLPETFD 250
    RLKELAEKMG FRHVESGPYV RSSYHAAGYG GGTRTDQPVA SGCLSDQEGV 300
    SAQ 303
    Length:303
    Mass (Da):32,887
    Last modified:May 10, 2004 - v1
    Checksum:iB63BE8FCEE0706C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017180 Genomic DNA. Translation: AAR33712.1.
    RefSeqiNP_951439.1. NC_002939.5.

    Genome annotation databases

    EnsemblBacteriaiAAR33712; AAR33712; GSU0380.
    GeneIDi2686621.
    KEGGigsu:GSU0380.
    PATRICi22023504. VBIGeoSul17553_0378.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017180 Genomic DNA. Translation: AAR33712.1 .
    RefSeqi NP_951439.1. NC_002939.5.

    3D structure databases

    ProteinModelPortali P61196.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243231.GSU0380.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAR33712 ; AAR33712 ; GSU0380 .
    GeneIDi 2686621.
    KEGGi gsu:GSU0380.
    PATRICi 22023504. VBIGeoSul17553_0378.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi ITRRPEW.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci GSUL243231:GH27-385-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51573 / DSM 12127 / PCA.

    Entry informationi

    Entry nameiLIPA_GEOSL
    AccessioniPrimary (citable) accession number: P61196
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3