P61177 (RL22_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L22

Gene names

Name:	rplV
Ordered Locus Names:	Z4686, ECs4180

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g., L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome By similarity. HAMAP MF_01331_B The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome By similarity. HAMAP MF_01331_B
Subunit structure	Part of the 50S ribosomal subunit.
Sequence similarities	Belongs to the ribosomal protein L22P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 110	110	50S ribosomal protein L22 HAMAP MF_01331_B		PRO_0000125155

Sequences

	Sequence		Length	Mass (Da)
	P61177 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: F0E0C6982772277C	FASTA	110	12,226
	10 20 30 40 50 60 METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH 70 80 90 100 110 NDGADIDDLK VTKIFVDEGP SMKRIMPRAK GRADRILKRT SHITVVVSDR

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58436.1. BA000007 Genomic DNA. Translation: BAB37603.1.
PIR	D91151. H85996.
RefSeq	NP_289876.1. NC_002655.2. NP_312207.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P61177.
SMR	P61177. Positions 1-110.
ModBase	Search...
Proteomic databases
PRIDE	P61177.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000028449; EBESCP00000027342; EBESCG00000027500. EBESCT00000056231; EBESCP00000054059; EBESCG00000055279.
GeneID	915968. 958554.
GenomeReviews	Gene locus Z4686 in contig AE005174_GR. Gene locus ECs4180 in contig BA000007_GR.
KEGG	ece:Z4686. ecs:ECs4180.
PATRIC	18357908. VBIEscCol44059_4121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010309.
HOGENOM	HBG568696.
OMA	AILKFAP.
ProtClustDB	PRK00565.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4180-MONOMER.
Family and domain databases
HAMAP	MF_01331_B. Ribosomal_L22_B. [Tree]
InterPro	IPR001063. Ribosomal_L22. IPR018260. Ribosomal_L22/L17_CS. IPR005727. Ribosomal_L22_bac-type. [Graphical view]
Gene3D	G3DSA:3.90.470.10. Ribosomal_L22. 1 hit.
KO	K02890.
PANTHER	PTHR13501. Ribosom_L22_bac. 1 hit.
Pfam	PF00237. Ribosomal_L22. 1 hit. [Graphical view]
SUPFAM	SSF54843. Ribosomal_L22. 1 hit.
TIGRFAMs	TIGR01044. RplV_bact. 1 hit.
PROSITE	PS00464. RIBOSOMAL_L22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00207. Azithromycin. DB00199. Erythromycin.

Entry information

Entry name

RL22_ECO57

Accession

Primary (citable) accession number: P61177
Secondary accession number(s): P02423

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program