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Protein

D(2) dopamine receptor

Gene

Drd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114AgonistBy similarity1
Sitei194Important for receptor activation1
Sitei197Important for receptor activation1

GO - Molecular functioni

  • dopamine binding Source: RGD
  • dopamine neurotransmitter receptor activity Source: RGD
  • dopamine neurotransmitter receptor activity, coupled via Gi/Go Source: BHF-UCL
  • drug binding Source: BHF-UCL
  • G-protein coupled receptor activity Source: RGD
  • identical protein binding Source: RGD
  • ionotropic glutamate receptor binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • receptor binding Source: RGD

GO - Biological processi

  • acid secretion Source: BHF-UCL
  • activation of protein kinase activity Source: RGD
  • adenohypophysis development Source: RGD
  • adenylate cyclase-inhibiting dopamine receptor signaling pathway Source: BHF-UCL
  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RGD
  • adult behavior Source: RGD
  • adult walking behavior Source: RGD
  • arachidonic acid secretion Source: RGD
  • associative learning Source: RGD
  • auditory behavior Source: RGD
  • axonogenesis Source: RGD
  • behavioral response to cocaine Source: RGD
  • behavioral response to ethanol Source: RGD
  • branching morphogenesis of a nerve Source: RGD
  • cellular potassium ion homeostasis Source: BHF-UCL
  • cerebral cortex GABAergic interneuron migration Source: RGD
  • circadian regulation of gene expression Source: RGD
  • dopamine metabolic process Source: RGD
  • dopamine receptor signaling pathway Source: RGD
  • feeding behavior Source: RGD
  • forebrain development Source: RGD
  • G-protein coupled receptor internalization Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • grooming behavior Source: RGD
  • intracellular signal transduction Source: RGD
  • locomotory behavior Source: RGD
  • long-term memory Source: RGD
  • modulation of chemical synaptic transmission Source: RGD
  • negative regulation of adenylate cyclase activity Source: BHF-UCL
  • negative regulation of blood pressure Source: RGD
  • negative regulation of cell migration Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of circadian sleep/wake cycle, sleep Source: RGD
  • negative regulation of cytosolic calcium ion concentration Source: RGD
  • negative regulation of dopamine receptor signaling pathway Source: RGD
  • negative regulation of dopamine secretion Source: RGD
  • negative regulation of innate immune response Source: RGD
  • negative regulation of insulin secretion Source: RGD
  • negative regulation of protein kinase B signaling Source: RGD
  • negative regulation of protein secretion Source: RGD
  • negative regulation of synaptic transmission, glutamatergic Source: RGD
  • negative regulation of voltage-gated calcium channel activity Source: BHF-UCL
  • neurological system process involved in regulation of systemic arterial blood pressure Source: RGD
  • neuron-neuron synaptic transmission Source: RGD
  • orbitofrontal cortex development Source: RGD
  • peristalsis Source: RGD
  • phosphatidylinositol metabolic process Source: BHF-UCL
  • phospholipase C-activating dopamine receptor signaling pathway Source: RGD
  • pigmentation Source: RGD
  • positive regulation of cytokinesis Source: RGD
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: RGD
  • positive regulation of dopamine uptake involved in synaptic transmission Source: RGD
  • positive regulation of ERK1 and ERK2 cascade Source: RGD
  • positive regulation of glial cell-derived neurotrophic factor secretion Source: RGD
  • positive regulation of G-protein coupled receptor protein signaling pathway Source: RGD
  • positive regulation of growth hormone secretion Source: RGD
  • positive regulation of long-term synaptic potentiation Source: RGD
  • positive regulation of mitotic nuclear division Source: BHF-UCL
  • positive regulation of multicellular organism growth Source: RGD
  • positive regulation of neuroblast proliferation Source: RGD
  • positive regulation of receptor internalization Source: RGD
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • positive regulation of urine volume Source: RGD
  • prepulse inhibition Source: RGD
  • protein localization Source: RGD
  • regulation of cAMP metabolic process Source: RGD
  • regulation of dopamine secretion Source: RGD
  • regulation of dopamine uptake involved in synaptic transmission Source: RGD
  • regulation of heart rate Source: RGD
  • regulation of locomotion involved in locomotory behavior Source: RGD
  • regulation of long-term neuronal synaptic plasticity Source: RGD
  • regulation of MAPK cascade Source: RGD
  • regulation of phosphoprotein phosphatase activity Source: RGD
  • regulation of potassium ion transport Source: BHF-UCL
  • regulation of sodium ion transport Source: RGD
  • regulation of synapse structural plasticity Source: RGD
  • regulation of synaptic transmission, GABAergic Source: RGD
  • release of sequestered calcium ion into cytosol Source: BHF-UCL
  • response to amphetamine Source: RGD
  • response to axon injury Source: RGD
  • response to cocaine Source: RGD
  • response to drug Source: BHF-UCL
  • response to ethanol Source: RGD
  • response to histamine Source: RGD
  • response to hypoxia Source: RGD
  • response to inactivity Source: RGD
  • response to iron ion Source: RGD
  • response to light stimulus Source: RGD
  • response to morphine Source: RGD
  • response to nicotine Source: RGD
  • response to toxic substance Source: RGD
  • sensory perception of smell Source: RGD
  • startle response Source: RGD
  • striatum development Source: RGD
  • synapse assembly Source: BHF-UCL
  • synaptic transmission, dopaminergic Source: RGD
  • temperature homeostasis Source: RGD
  • visual learning Source: RGD
  • Wnt signaling pathway Source: RGD

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390651. Dopamine receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
D(2) dopamine receptor
Alternative name(s):
Dopamine D2 receptor
Gene namesi
Name:Drd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2520. Drd2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37ExtracellularBy similarityAdd BLAST37
Transmembranei38 – 60Helical; Name=1By similarityAdd BLAST23
Topological domaini61 – 70CytoplasmicBy similarity10
Transmembranei71 – 93Helical; Name=2By similarityAdd BLAST23
Topological domaini94 – 108ExtracellularBy similarityAdd BLAST15
Transmembranei109 – 130Helical; Name=3By similarityAdd BLAST22
Topological domaini131 – 151CytoplasmicBy similarityAdd BLAST21
Transmembranei152 – 172Helical; Name=4By similarityAdd BLAST21
Topological domaini173 – 188ExtracellularBy similarityAdd BLAST16
Transmembranei189 – 213Helical; Name=5By similarityAdd BLAST25
Topological domaini214 – 374CytoplasmicBy similarityAdd BLAST161
Transmembranei375 – 396Helical; Name=6By similarityAdd BLAST22
Topological domaini397 – 410ExtracellularBy similarityAdd BLAST14
Transmembranei411 – 432Helical; Name=7By similarityAdd BLAST22
Topological domaini433 – 444CytoplasmicBy similarityAdd BLAST12

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi193S → A: Moderate decrease in ligand binding. 200-fold reduction of agonist-mediated cAMP inhibition. 1 Publication1
Mutagenesisi194S → A: Small decrease in agonist binding. Complete loss of agonist-mediated cAMP inhibition. 1 Publication1
Mutagenesisi197S → A: Small decrease in agonist binding. 18-fold reduction of agonist-mediated cAMP inhibition. 1 Publication1
Mutagenesisi420S → A: Moderate decrease in ligand binding. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL339.
GuidetoPHARMACOLOGYi215.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000693901 – 444D(2) dopamine receptorAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi17N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi23N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi107 ↔ 182PROSITE-ProRule annotation
Disulfide bondi400 ↔ 402PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP61169.

PTM databases

iPTMnetiP61169.
PhosphoSitePlusiP61169.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008428.
GenevisibleiP61169. RN.

Interactioni

Subunit structurei

Forms homo- and heterooligomers with DRD4. The interaction with DRD4 may modulate agonist-induced downstream signaling (By similarity). Interacts with HTR2A, CADPS and CADPS2 (By similarity). Interacts with KCNA2 (By similarity). Interacts with GPRASP1, PPP1R9B and CLIC6. Interacts with ARRB2.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ppp1r9bO352742EBI-80012,EBI-80022

GO - Molecular functioni

  • identical protein binding Source: RGD
  • ionotropic glutamate receptor binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

BioGridi246498. 5 interactors.
CORUMiP61169.
DIPiDIP-30883N.
IntActiP61169. 4 interactors.
MINTiMINT-4792453.

Chemistry databases

BindingDBiP61169.

Structurei

3D structure databases

ProteinModelPortaliP61169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 197Agonist bindingBy similarity8
Regioni211 – 374Interaction with PPP1R9B1 PublicationAdd BLAST164
Regioni387 – 395Agonist bindingBy similarity9

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00890000139331.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP61169.
KOiK04145.
OMAiRNWSRPF.
OrthoDBiEOG091G06VI.
PhylomeDBiP61169.
TreeFamiTF334382.

Family and domain databases

InterProiView protein in InterPro
IPR001922. Dopamine_D2_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00567. DOPAMINED2R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P61169-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPLNLSWYD DDLERQNWSR PFNGSEGKAD RPHYNYYAML LTLLIFIIVF
60 70 80 90 100
GNVLVCMAVS REKALQTTTN YLIVSLAVAD LLVATLVMPW VVYLEVVGEW
110 120 130 140 150
KFSRIHCDIF VTLDVMMCTA SILNLCAISI DRYTAVAMPM LYNTRYSSKR
160 170 180 190 200
RVTVMIAIVW VLSFTISCPL LFGLNNTDQN ECIIANPAFV VYSSIVSFYV
210 220 230 240 250
PFIVTLLVYI KIYIVLRKRR KRVNTKRSSR AFRANLKTPL KGNCTHPEDM
260 270 280 290 300
KLCTVIMKSN GSFPVNRRRM DAARRAQELE MEMLSSTSPP ERTRYSPIPP
310 320 330 340 350
SHHQLTLPDP SHHGLHSNPD SPAKPEKNGH AKIVNPRIAK FFEIQTMPNG
360 370 380 390 400
KTRTSLKTMS RRKLSQQKEK KATQMLAIVL GVFIICWLPF FITHILNIHC
410 420 430 440
DCNIPPVLYS AFTWLGYVNS AVNPIIYTTF NIEFRKAFMK ILHC
Length:444
Mass (Da):50,904
Last modified:May 10, 2004 - v1
Checksum:i216E56CEE5CA32FB
GO
Isoform Short (identifier: P61169-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     242-270: Missing.

Show »
Length:415
Mass (Da):47,599
Checksum:i2923089F3EDAC911
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99E → D in CAA37373 (PubMed:2139615).Curated1
Sequence conflicti173G → R in CAA37373 (PubMed:2139615).Curated1
Sequence conflicti180N → G in CAA37373 (PubMed:2139615).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001871242 – 270Missing in isoform Short. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36831 mRNA. Translation: AAA41075.1.
X53278 mRNA. Translation: CAA37373.1.
X56065 mRNA. Translation: CAA39543.1.
M32241 mRNA. Translation: AAA41074.1.
PIRiS08146.
RefSeqiNP_036679.1. NM_012547.1. [P61169-1]
XP_006243041.1. XM_006242979.3. [P61169-2]
UniGeneiRn.87299.

Genome annotation databases

EnsembliENSRNOT00000045944; ENSRNOP00000043759; ENSRNOG00000008428. [P61169-1]
ENSRNOT00000083419; ENSRNOP00000074235; ENSRNOG00000008428. [P61169-2]
GeneIDi24318.
KEGGirno:24318.
UCSCiRGD:2520. rat.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDRD2_RAT
AccessioniPrimary (citable) accession number: P61169
Secondary accession number(s): P13953
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 22, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families