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P61168 (DRD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D(2) dopamine receptor
Alternative name(s):
Dopamine D2 receptor
Gene names
Name:Drd2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase By similarity.

Subunit structure

Forms homo- and heterooligomers with DRD4. The interaction with DRD4 may modulate agonist-induced downstream signaling. Interacts with GPRASP1, PPP1R9B, CADPS, CADPS2 and CLIC6. Interacts with ARRB2, HTR2A and GNAI1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Isoform 1 and isoform 2 are expressed in the pituitary and brain. Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor internalization

Inferred from electronic annotation. Source: Ensembl

G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 14712229. Source: MGI

Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

activation of protein kinase activity

Inferred from electronic annotation. Source: Ensembl

adenohypophysis development

Inferred from mutant phenotype PubMed 9140068. Source: MGI

adenylate cyclase-inhibiting dopamine receptor signaling pathway

Inferred from direct assay PubMed 12665504. Source: MGI

adult behavior

Inferred from mutant phenotype PubMed 11303741. Source: MGI

adult walking behavior

Inferred from mutant phenotype PubMed 7566118. Source: MGI

arachidonic acid secretion

Inferred from electronic annotation. Source: Ensembl

associative learning

Inferred from mutant phenotype PubMed 12084937PubMed 12182883PubMed 15061865. Source: MGI

auditory behavior

Inferred from electronic annotation. Source: Ensembl

axonogenesis

Inferred from mutant phenotype PubMed 11438590. Source: MGI

behavioral response to cocaine

Inferred from mutant phenotype PubMed 17426149. Source: MGI

behavioral response to ethanol

Inferred from mutant phenotype PubMed 11105945PubMed 11166059PubMed 12837020. Source: MGI

branching morphogenesis of a nerve

Inferred from mutant phenotype PubMed 11438590. Source: MGI

cerebral cortex GABAergic interneuron migration

Inferred from mutant phenotype PubMed 17409246. Source: MGI

circadian regulation of gene expression

Inferred from mutant phenotype PubMed 16606840. Source: MGI

dopamine metabolic process

Inferred from mutant phenotype PubMed 7925842. Source: MGI

dopamine receptor signaling pathway

Inferred from genetic interaction PubMed 11089973. Source: MGI

feeding behavior

Inferred from genetic interaction PubMed 15272078. Source: MGI

grooming behavior

Inferred from mutant phenotype PubMed 10698004. Source: MGI

intracellular signal transduction

Inferred from electronic annotation. Source: Ensembl

locomotory behavior

Inferred from mutant phenotype PubMed 10471093PubMed 10698004PubMed 11069937PubMed 11166059PubMed 11557166PubMed 12084937PubMed 12175868PubMed 12185400PubMed 12837020PubMed 15080900PubMed 9547254. Source: MGI

long-term memory

Inferred from electronic annotation. Source: Ensembl

negative regulation of adenylate cyclase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of blood pressure

Inferred from mutant phenotype PubMed 11566895. Source: MGI

negative regulation of cell migration

Inferred from mutant phenotype PubMed 17409246. Source: MGI

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 10537166PubMed 9140068PubMed 9247267PubMed 9247268PubMed 9717839. Source: MGI

negative regulation of circadian sleep/wake cycle, sleep

Inferred from electronic annotation. Source: Ensembl

negative regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

negative regulation of dopamine receptor signaling pathway

Inferred from mutant phenotype PubMed 11089973. Source: MGI

negative regulation of dopamine secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein kinase B signaling

Inferred from mutant phenotype PubMed 17251429. Source: MGI

negative regulation of protein secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of synaptic transmission, glutamatergic

Inferred from mutant phenotype PubMed 11160501PubMed 15489038. Source: MGI

negative regulation of voltage-gated calcium channel activity

Inferred from electronic annotation. Source: Ensembl

neurological system process involved in regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 11566895. Source: MGI

neuron-neuron synaptic transmission

Inferred from mutant phenotype PubMed 12084937. Source: MGI

orbitofrontal cortex development

Inferred from electronic annotation. Source: Ensembl

peristalsis

Inferred from mutant phenotype PubMed 16525059. Source: MGI

phosphatidylinositol metabolic process

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating dopamine receptor signaling pathway

Inferred from direct assay PubMed 15016423. Source: MGI

pigmentation

Inferred from mutant phenotype PubMed 9140068. Source: MGI

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of G-protein coupled receptor protein signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of dopamine uptake involved in synaptic transmission

Inferred from mutant phenotype PubMed 9886065. Source: MGI

positive regulation of growth hormone secretion

Inferred from mutant phenotype PubMed 11897683. Source: MGI

positive regulation of long-term synaptic potentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 11897683. Source: MGI

positive regulation of neuroblast proliferation

Inferred from direct assay PubMed 17426594. Source: MGI

positive regulation of receptor internalization

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from mutant phenotype PubMed 11566895. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of urine volume

Inferred from mutant phenotype PubMed 11566895. Source: MGI

prepulse inhibition

Inferred from mutant phenotype PubMed 10341260PubMed 12417685PubMed 12476322PubMed 17712281. Source: MGI

protein localization

Inferred from mutant phenotype PubMed 10828532. Source: MGI

regulation of dopamine secretion

Inferred from mutant phenotype PubMed 11943831. Source: MGI

regulation of dopamine uptake involved in synaptic transmission

Inferred from mutant phenotype PubMed 12223553. Source: MGI

regulation of heart rate

Inferred from mutant phenotype PubMed 11566895. Source: MGI

regulation of locomotion involved in locomotory behavior

Inferred from electronic annotation. Source: Ensembl

regulation of long-term neuronal synaptic plasticity

Inferred from mutant phenotype PubMed 9169514. Source: MGI

regulation of phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: Ensembl

regulation of potassium ion transport

Inferred from mutant phenotype PubMed 11566895. Source: MGI

regulation of sodium ion transport

Inferred from mutant phenotype PubMed 11566895. Source: MGI

regulation of synapse structural plasticity

Inferred from electronic annotation. Source: Ensembl

regulation of synaptic transmission

Inferred from mutant phenotype PubMed 10974427. Source: MGI

regulation of synaptic transmission, GABAergic

Inferred from mutant phenotype PubMed 15080900PubMed 15489038. Source: MGI

release of sequestered calcium ion into cytosol

Inferred from electronic annotation. Source: Ensembl

response to amphetamine

Inferred from mutant phenotype PubMed 12476322PubMed 18486343. Source: MGI

response to axon injury

Inferred from electronic annotation. Source: Ensembl

response to cocaine

Inferred from direct assay PubMed 18671743. Source: MGI

response to drug

Inferred from direct assay PubMed 7549459. Source: MGI

response to histamine

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to inactivity

Inferred from electronic annotation. Source: Ensembl

response to iron ion

Inferred from electronic annotation. Source: Ensembl

response to light stimulus

Inferred from mutant phenotype PubMed 16715079. Source: MGI

response to morphine

Inferred from mutant phenotype PubMed 11264672PubMed 11567069PubMed 11943831PubMed 12182883PubMed 9252189. Source: MGI

response to nicotine

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from electronic annotation. Source: Ensembl

sensory perception of smell

Inferred from mutant phenotype PubMed 16765459. Source: MGI

startle response

Inferred from mutant phenotype PubMed 17712281. Source: MGI

striatum development

Inferred from electronic annotation. Source: Ensembl

synapse assembly

Inferred from electronic annotation. Source: Ensembl

synaptic transmission, dopaminergic

Inferred from electronic annotation. Source: Ensembl

temperature homeostasis

Inferred from mutant phenotype PubMed 10471093PubMed 18486343. Source: MGI

visual learning

Inferred from mutant phenotype PubMed 12097513. Source: MGI

   Cellular_componentacrosomal vesicle

Inferred from electronic annotation. Source: Ensembl

axon

Inferred from direct assay PubMed 10051229. Source: MGI

axon terminus

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from direct assay PubMed 10051229. Source: MGI

dendritic spine

Inferred from electronic annotation. Source: Ensembl

endocytic vesicle

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

lateral plasma membrane

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from direct assay PubMed 7706278. Source: MGI

perikaryon

Inferred from electronic annotation. Source: Ensembl

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

sperm flagellum

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionG-protein coupled receptor activity

Inferred from direct assay PubMed 14712229. Source: MGI

adrenergic receptor activity

Inferred from electronic annotation. Source: InterPro

dopamine binding

Inferred from direct assay PubMed 15716360. Source: MGI

dopamine neurotransmitter receptor activity

Inferred from direct assay PubMed 18671743. Source: MGI

dopamine neurotransmitter receptor activity, coupled via Gi/Go

Inferred from direct assay PubMed 11015197PubMed 11069937PubMed 12476322PubMed 12930822PubMed 1980140Ref.1PubMed 7566118PubMed 7706278PubMed 8001143PubMed 8294904PubMed 8836575. Source: MGI

drug binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P61168-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P61168-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     242-270: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444D(2) dopamine receptor
PRO_0000069388

Regions

Topological domain1 – 3737Extracellular By similarity
Transmembrane38 – 6023Helical; Name=1; By similarity
Topological domain61 – 7010Cytoplasmic By similarity
Transmembrane71 – 9323Helical; Name=2; By similarity
Topological domain94 – 10815Extracellular By similarity
Transmembrane109 – 13022Helical; Name=3; By similarity
Topological domain131 – 15121Cytoplasmic By similarity
Transmembrane152 – 17221Helical; Name=4; By similarity
Topological domain173 – 18816Extracellular By similarity
Transmembrane189 – 21325Helical; Name=5; By similarity
Topological domain214 – 374161Cytoplasmic By similarity
Transmembrane375 – 39622Helical; Name=6; By similarity
Topological domain397 – 41014Extracellular By similarity
Transmembrane411 – 43222Helical; Name=7; By similarity
Topological domain433 – 44412Cytoplasmic By similarity
Region190 – 1978Agonist binding By similarity
Region211 – 374164Interaction with PPP1R9B By similarity
Region387 – 3959Agonist binding By similarity

Sites

Binding site1141Agonist By similarity
Site1941Important for receptor activation
Site1971Important for receptor activation

Amino acid modifications

Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation171N-linked (GlcNAc...) Potential
Glycosylation231N-linked (GlcNAc...) Potential
Disulfide bond107 ↔ 182 By similarity
Disulfide bond400 ↔ 402 By similarity

Natural variations

Alternative sequence242 – 27029Missing in isoform 2.
VSP_010241

Experimental info

Sequence conflict291P → A in CAA39209. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 2EC8460CA11FC7F0

FASTA44450,930
        10         20         30         40         50         60 
MDPLNLSWYD DDLERQNWSR PFNGSEGKPD RPHYNYYAML LTLLIFIIVF GNVLVCMAVS 

        70         80         90        100        110        120 
REKALQTTTN YLIVSLAVAD LLVATLVMPW VVYLEVVGEW KFSRIHCDIF VTLDVMMCTA 

       130        140        150        160        170        180 
SILNLCAISI DRYTAVAMPM LYNTRYSSKR RVTVMIAIVW VLSFTISCPL LFGLNNTDQN 

       190        200        210        220        230        240 
ECIIANPAFV VYSSIVSFYV PFIVTLLVYI KIYIVLRKRR KRVNTKRSSR AFRANLKTPL 

       250        260        270        280        290        300 
KGNCTHPEDM KLCTVIMKSN GSFPVNRRRM DAARRAQELE MEMLSSTSPP ERTRYSPIPP 

       310        320        330        340        350        360 
SHHQLTLPDP SHHGLHSNPD SPAKPEKNGH AKIVNPRIAK FFEIQTMPNG KTRTSLKTMS 

       370        380        390        400        410        420 
RRKLSQQKEK KATQMLAIVL GVFIICWLPF FITHILNIHC DCNIPPVLYS AFTWLGYVNS 

       430        440 
AVNPIIYTTF NIEFRKAFMK ILHC 

« Hide

Isoform 2 (Short) [UniParc] [UniParc].

Checksum: 645E7A47407FCFCD
Show »

FASTA41547,625

References

« Hide 'large scale' references
[1]"Differential expression of the mouse D2 dopamine receptor isoforms."
Montmayeur J.P., Bausero P., Amlaiky N., Maroteaux L., Hen R., Borrelli E.
FEBS Lett. 278:239-243(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]"A second molecular form of D2 dopamine receptor in rat and bovine caudate nucleus."
Chio C.L., Hess G.F., Graham R.S., Huff R.M.
Nature 343:266-269(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"The dopamine D2 receptor: two molecular forms generated by alternative splicing."
Dal-Toso R., Sommer B., Ewert M., Herb A., Pritchett D.B., Bach A., Shivers B.D., Seeburg P.H.
EMBO J. 8:4025-4034(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-270 (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55674 mRNA. Translation: CAA39209.1.
CH466522 Genomic DNA. Translation: EDL25723.1.
BC105663 mRNA. Translation: AAI05664.1.
BC105664 mRNA. Translation: AAI05665.1.
BC105665 mRNA. Translation: AAI05666.1.
BC105666 mRNA. Translation: AAI05667.1.
CCDSCCDS40615.1.
PIRDYMSD2. S13921.
RefSeqNP_034207.2. NM_010077.2. [P61168-1]
XP_006510059.1. XM_006509996.1. [P61168-2]
UniGeneMm.41970.

3D structure databases

ProteinModelPortalP61168.
SMRP61168. Positions 37-444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199306. 1 interaction.
IntActP61168. 4 interactions.
MINTMINT-5313868.
STRING10090.ENSMUSP00000075170.

Chemistry

BindingDBP61168.
ChEMBLCHEMBL3427.
GuidetoPHARMACOLOGY215.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP61168.

Proteomic databases

PRIDEP61168.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075764; ENSMUSP00000075170; ENSMUSG00000032259. [P61168-1]
GeneID13489.
KEGGmmu:13489.
UCSCuc009pja.1. mouse.

Organism-specific databases

CTD1813.
MGIMGI:94924. Drd2.

Phylogenomic databases

eggNOGNOG309657.
GeneTreeENSGT00750000117240.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidQ0VGH9.
KOK04145.
OMARNWSRPF.
OrthoDBEOG769ZMG.
TreeFamTF334382.

Gene expression databases

BgeeP61168.
CleanExMM_DRD2.
GenevestigatorP61168.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR001922. Dopamine_D2_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00567. DOPAMINED2R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284001.
PROP61168.
SOURCESearch...

Entry information

Entry nameDRD2_MOUSE
AccessionPrimary (citable) accession number: P61168
Secondary accession number(s): P13953, Q0VGH9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries