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Protein

D(2) dopamine receptor

Gene

Drd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141AgonistBy similarity
Sitei194 – 1941Important for receptor activation
Sitei197 – 1971Important for receptor activation

GO - Molecular functioni

  • dopamine binding Source: MGI
  • dopamine neurotransmitter receptor activity Source: MGI
  • dopamine neurotransmitter receptor activity, coupled via Gi/Go Source: MGI
  • drug binding Source: MGI
  • G-protein coupled receptor activity Source: MGI
  • identical protein binding Source: MGI

GO - Biological processi

  • activation of protein kinase activity Source: Ensembl
  • adenohypophysis development Source: MGI
  • adenylate cyclase-inhibiting dopamine receptor signaling pathway Source: MGI
  • adult behavior Source: MGI
  • adult walking behavior Source: MGI
  • arachidonic acid secretion Source: MGI
  • associative learning Source: MGI
  • auditory behavior Source: Ensembl
  • axonogenesis Source: MGI
  • behavioral response to cocaine Source: MGI
  • behavioral response to ethanol Source: MGI
  • branching morphogenesis of a nerve Source: MGI
  • cerebral cortex GABAergic interneuron migration Source: MGI
  • circadian regulation of gene expression Source: MGI
  • dopamine metabolic process Source: MGI
  • dopamine receptor signaling pathway Source: MGI
  • feeding behavior Source: MGI
  • G-protein coupled receptor internalization Source: Ensembl
  • G-protein coupled receptor signaling pathway Source: MGI
  • grooming behavior Source: MGI
  • intracellular signal transduction Source: MGI
  • locomotory behavior Source: MGI
  • long-term memory Source: Ensembl
  • negative regulation of adenylate cyclase activity Source: MGI
  • negative regulation of blood pressure Source: MGI
  • negative regulation of cell migration Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of circadian sleep/wake cycle, sleep Source: Ensembl
  • negative regulation of cytosolic calcium ion concentration Source: GO_Central
  • negative regulation of dopamine receptor signaling pathway Source: MGI
  • negative regulation of dopamine secretion Source: Ensembl
  • negative regulation of innate immune response Source: CACAO
  • negative regulation of insulin secretion Source: Ensembl
  • negative regulation of protein kinase B signaling Source: MGI
  • negative regulation of protein secretion Source: MGI
  • negative regulation of synaptic transmission, glutamatergic Source: MGI
  • negative regulation of voltage-gated calcium channel activity Source: MGI
  • neurological system process involved in regulation of systemic arterial blood pressure Source: MGI
  • neuron-neuron synaptic transmission Source: MGI
  • orbitofrontal cortex development Source: Ensembl
  • peristalsis Source: MGI
  • phosphatidylinositol metabolic process Source: Ensembl
  • phospholipase C-activating dopamine receptor signaling pathway Source: MGI
  • pigmentation Source: MGI
  • positive regulation of cytokinesis Source: MGI
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: MGI
  • positive regulation of dopamine uptake involved in synaptic transmission Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  • positive regulation of glial cell line-derived neurotrophic factor secretion Source: MGI
  • positive regulation of G-protein coupled receptor protein signaling pathway Source: Ensembl
  • positive regulation of growth hormone secretion Source: MGI
  • positive regulation of long-term synaptic potentiation Source: Ensembl
  • positive regulation of multicellular organism growth Source: MGI
  • positive regulation of neuroblast proliferation Source: MGI
  • positive regulation of receptor internalization Source: Ensembl
  • positive regulation of renal sodium excretion Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • positive regulation of urine volume Source: MGI
  • prepulse inhibition Source: MGI
  • protein localization Source: MGI
  • regulation of cAMP metabolic process Source: MGI
  • regulation of dopamine secretion Source: MGI
  • regulation of dopamine uptake involved in synaptic transmission Source: MGI
  • regulation of heart rate Source: MGI
  • regulation of locomotion involved in locomotory behavior Source: Ensembl
  • regulation of long-term neuronal synaptic plasticity Source: MGI
  • regulation of phosphoprotein phosphatase activity Source: Ensembl
  • regulation of potassium ion transport Source: MGI
  • regulation of sodium ion transport Source: MGI
  • regulation of synapse structural plasticity Source: Ensembl
  • regulation of synaptic transmission Source: MGI
  • regulation of synaptic transmission, GABAergic Source: MGI
  • release of sequestered calcium ion into cytosol Source: Ensembl
  • response to amphetamine Source: MGI
  • response to axon injury Source: Ensembl
  • response to cocaine Source: MGI
  • response to drug Source: MGI
  • response to histamine Source: MGI
  • response to hypoxia Source: Ensembl
  • response to inactivity Source: Ensembl
  • response to iron ion Source: Ensembl
  • response to light stimulus Source: MGI
  • response to morphine Source: MGI
  • response to nicotine Source: Ensembl
  • response to toxic substance Source: GO_Central
  • sensory perception of smell Source: MGI
  • startle response Source: MGI
  • striatum development Source: Ensembl
  • synapse assembly Source: Ensembl
  • synaptic transmission, dopaminergic Source: Ensembl
  • temperature homeostasis Source: MGI
  • visual learning Source: MGI
  • Wnt signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_294218. Dopamine receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
D(2) dopamine receptor
Alternative name(s):
Dopamine D2 receptor
Gene namesi
Name:Drd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:94924. Drd2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737ExtracellularBy similarityAdd
BLAST
Transmembranei38 – 6023Helical; Name=1By similarityAdd
BLAST
Topological domaini61 – 7010CytoplasmicBy similarity
Transmembranei71 – 9323Helical; Name=2By similarityAdd
BLAST
Topological domaini94 – 10815ExtracellularBy similarityAdd
BLAST
Transmembranei109 – 13022Helical; Name=3By similarityAdd
BLAST
Topological domaini131 – 15121CytoplasmicBy similarityAdd
BLAST
Transmembranei152 – 17221Helical; Name=4By similarityAdd
BLAST
Topological domaini173 – 18816ExtracellularBy similarityAdd
BLAST
Transmembranei189 – 21325Helical; Name=5By similarityAdd
BLAST
Topological domaini214 – 374161CytoplasmicBy similarityAdd
BLAST
Transmembranei375 – 39622Helical; Name=6By similarityAdd
BLAST
Topological domaini397 – 41014ExtracellularBy similarityAdd
BLAST
Transmembranei411 – 43222Helical; Name=7By similarityAdd
BLAST
Topological domaini433 – 44412CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • acrosomal vesicle Source: Ensembl
  • axon Source: MGI
  • axon terminus Source: Ensembl
  • dendrite Source: MGI
  • dendritic spine Source: Ensembl
  • endocytic vesicle Source: Ensembl
  • integral component of plasma membrane Source: MGI
  • lateral plasma membrane Source: Ensembl
  • membrane Source: MGI
  • perikaryon Source: Ensembl
  • postsynaptic density Source: Ensembl
  • sperm flagellum Source: Ensembl
  • synaptic vesicle membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444D(2) dopamine receptorPRO_0000069388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi17 – 171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi107 ↔ 182PROSITE-ProRule annotation
Disulfide bondi400 ↔ 402PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP61168.

PTM databases

PhosphoSiteiP61168.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in the pituitary and brain. Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Gene expression databases

BgeeiP61168.
CleanExiMM_DRD2.
GenevestigatoriP61168.

Interactioni

Subunit structurei

Forms homo- and heterooligomers with DRD4. The interaction with DRD4 may modulate agonist-induced downstream signaling. Interacts with GPRASP1, PPP1R9B, CADPS, CADPS2 and CLIC6. Interacts with ARRB2, HTR2A and GNAI1 (By similarity). Isoform 1 and isoform 2 interact both with KCNA2.By similarity1 Publication

Protein-protein interaction databases

BioGridi199306. 1 interaction.
IntActiP61168. 4 interactions.
MINTiMINT-5313868.
STRINGi10090.ENSMUSP00000075170.

Structurei

3D structure databases

ProteinModelPortaliP61168.
SMRiP61168. Positions 37-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 1978Agonist bindingBy similarity
Regioni211 – 374164Interaction with PPP1R9BBy similarityAdd
BLAST
Regioni387 – 3959Agonist bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309657.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP61168.
KOiK04145.
OMAiRNWSRPF.
OrthoDBiEOG769ZMG.
TreeFamiTF334382.

Family and domain databases

InterProiIPR001922. Dopamine_D2_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00567. DOPAMINED2R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61168-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPLNLSWYD DDLERQNWSR PFNGSEGKPD RPHYNYYAML LTLLIFIIVF
60 70 80 90 100
GNVLVCMAVS REKALQTTTN YLIVSLAVAD LLVATLVMPW VVYLEVVGEW
110 120 130 140 150
KFSRIHCDIF VTLDVMMCTA SILNLCAISI DRYTAVAMPM LYNTRYSSKR
160 170 180 190 200
RVTVMIAIVW VLSFTISCPL LFGLNNTDQN ECIIANPAFV VYSSIVSFYV
210 220 230 240 250
PFIVTLLVYI KIYIVLRKRR KRVNTKRSSR AFRANLKTPL KGNCTHPEDM
260 270 280 290 300
KLCTVIMKSN GSFPVNRRRM DAARRAQELE MEMLSSTSPP ERTRYSPIPP
310 320 330 340 350
SHHQLTLPDP SHHGLHSNPD SPAKPEKNGH AKIVNPRIAK FFEIQTMPNG
360 370 380 390 400
KTRTSLKTMS RRKLSQQKEK KATQMLAIVL GVFIICWLPF FITHILNIHC
410 420 430 440
DCNIPPVLYS AFTWLGYVNS AVNPIIYTTF NIEFRKAFMK ILHC
Length:444
Mass (Da):50,930
Last modified:July 27, 2011 - v2
Checksum:i2EC8460CA11FC7F0
GO
Isoform 2 (identifier: P61168-2) [UniParc] [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     242-270: Missing.

Show »
Length:415
Mass (Da):47,625
Checksum:i645E7A47407FCFCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291P → A in CAA39209 (PubMed:1991517).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei242 – 27029Missing in isoform 2. 1 PublicationVSP_010241Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55674 mRNA. Translation: CAA39209.1.
CH466522 Genomic DNA. Translation: EDL25723.1.
BC105663 mRNA. Translation: AAI05664.1.
BC105664 mRNA. Translation: AAI05665.1.
BC105665 mRNA. Translation: AAI05666.1.
BC105666 mRNA. Translation: AAI05667.1.
CCDSiCCDS40615.1.
PIRiS13921. DYMSD2.
RefSeqiNP_034207.2. NM_010077.2. [P61168-1]
XP_006510059.1. XM_006509996.2. [P61168-2]
UniGeneiMm.41970.

Genome annotation databases

EnsembliENSMUST00000075764; ENSMUSP00000075170; ENSMUSG00000032259. [P61168-1]
GeneIDi13489.
KEGGimmu:13489.
UCSCiuc009pja.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55674 mRNA. Translation: CAA39209.1.
CH466522 Genomic DNA. Translation: EDL25723.1.
BC105663 mRNA. Translation: AAI05664.1.
BC105664 mRNA. Translation: AAI05665.1.
BC105665 mRNA. Translation: AAI05666.1.
BC105666 mRNA. Translation: AAI05667.1.
CCDSiCCDS40615.1.
PIRiS13921. DYMSD2.
RefSeqiNP_034207.2. NM_010077.2. [P61168-1]
XP_006510059.1. XM_006509996.2. [P61168-2]
UniGeneiMm.41970.

3D structure databases

ProteinModelPortaliP61168.
SMRiP61168. Positions 37-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199306. 1 interaction.
IntActiP61168. 4 interactions.
MINTiMINT-5313868.
STRINGi10090.ENSMUSP00000075170.

Chemistry

BindingDBiP61168.
ChEMBLiCHEMBL2109245.
GuidetoPHARMACOLOGYi215.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP61168.

Proteomic databases

PRIDEiP61168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075764; ENSMUSP00000075170; ENSMUSG00000032259. [P61168-1]
GeneIDi13489.
KEGGimmu:13489.
UCSCiuc009pja.1. mouse.

Organism-specific databases

CTDi1813.
MGIiMGI:94924. Drd2.

Phylogenomic databases

eggNOGiNOG309657.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP61168.
KOiK04145.
OMAiRNWSRPF.
OrthoDBiEOG769ZMG.
TreeFamiTF334382.

Enzyme and pathway databases

ReactomeiREACT_294218. Dopamine receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi284001.
PROiP61168.
SOURCEiSearch...

Gene expression databases

BgeeiP61168.
CleanExiMM_DRD2.
GenevestigatoriP61168.

Family and domain databases

InterProiIPR001922. Dopamine_D2_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00567. DOPAMINED2R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of the mouse D2 dopamine receptor isoforms."
    Montmayeur J.P., Bausero P., Amlaiky N., Maroteaux L., Hen R., Borrelli E.
    FEBS Lett. 278:239-243(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "A second molecular form of D2 dopamine receptor in rat and bovine caudate nucleus."
    Chio C.L., Hess G.F., Graham R.S., Huff R.M.
    Nature 343:266-269(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "The dopamine D2 receptor: two molecular forms generated by alternative splicing."
    Dal-Toso R., Sommer B., Ewert M., Herb A., Pritchett D.B., Bach A., Shivers B.D., Seeburg P.H.
    EMBO J. 8:4025-4034(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 175-270 (ISOFORM 1).
  6. "Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow."
    Fulton S., Thibault D., Mendez J.A., Lahaie N., Tirotta E., Borrelli E., Bouvier M., Tempel B.L., Trudeau L.E.
    J. Biol. Chem. 286:9360-9372(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNA2.

Entry informationi

Entry nameiDRD2_MOUSE
AccessioniPrimary (citable) accession number: P61168
Secondary accession number(s): P13953, Q0VGH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: July 27, 2011
Last modified: May 27, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.