Reviewed,
UniProtKB/Swiss-Prot P61160 (ARP2_HUMAN)
Last modified
November 3, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Actin-related protein 2 Alternative name(s): Actin-like protein 2 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 394 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Ref.5 |
| Subunit structure | Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. |
| Subcellular location | |
| Sequence similarities | Belongs to the actin family. ARP2 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell projection Cytoplasm Cytoskeleton |
| Ligand | ATP-binding Actin-binding Nucleotide-binding |
| PTM | Acetylation |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cell motion Ref.1 Traceable author statement. Source: UniProtKB |
| Cellular component | Arp2/3 protein complex Ref.1 Traceable author statement. Source: UniProtKB cell projectionInferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW actin bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ACTR3B | Q9P1U1 | 1 | EBI-353580,EBI-1047175 | |
| BAK1 | Q16611 | 1 | EBI-353580,EBI-519866 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 394 | 394 | Actin-related protein 2 | PRO_0000089067 | |||||
Regions | |||||||||
| Nucleotide binding | 160 – 162 | 3 | ATP By similarity | ||||||
| Nucleotide binding | 214 – 218 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 305 – 310 | 6 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 299 | 1 | N6-acetyllysine Ref.8 | ||||||
| Modified residue | 322 | 1 | N6-acetyllysine Ref.8 | ||||||
Experimental info | |||||||||
| Sequence conflict | 67 | 1 | M → T in AAH14546. Ref.4 | ||||||
| Sequence conflict | 172 | 1 | G → S in AAH14546. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." Welch M.D., Depace A.H., Verma S., Iwamatsu A., Mitchison T.J. J. Cell Biol. 138:375-384(1997) [PubMed: 9230079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle. |
| [5] | "Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes." Welch M.D., Iwamatsu A., Mitchison T.J. Nature 385:265-269(1997) [PubMed: 9000076] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION OF THE ARP2/3 COMPLEX, IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION. |
| [6] | "Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity." Gournier H., Goley E.D., Niederstrasser H., Trinh T., Welch M.D. Mol. Cell 8:1041-1052(2001) [PubMed: 11741539] [Abstract] Cited for: RECONSTITUTION OF THE ARP2/3 COMPLEX. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-299 AND LYS-322, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF006082 mRNA. Translation: AAB64187.1. AK315205 mRNA. Translation: BAG37642.1. CH471053 Genomic DNA. Translation: EAW99908.1. BC014546 mRNA. Translation: AAH14546.1. | |
| IPI | IPI00005159. |
| RefSeq | NP_001005386.1. NP_005713.1. |
| UniGene | Hs.643727 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1K8K based on UniProtKB P32391. |
| SMR | P61160. Positions 143-350. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P61160. 10 interactions. |
| STRING | P61160. |
PTM databases | |
| PhosphoSite | P61160. |
2-D gel databases | |
| SWISS-2DPAGE | P61160. |
Proteomic databases | |
| PRIDE | P61160. |
Genome annotation databases | |
| Ensembl | ENST00000260641; ENSP00000260641; ENSG00000138071; Homo sapiens. [Genome view] ENST00000377982; ENSP00000367220; ENSG00000138071; Homo sapiens. [Genome view] |
| GeneID | 10097. |
| UCSC | uc002sdq.1. human. |
Organism-specific databases | |
| CTD | 10097. |
| GeneCards | GC02P065366. |
| HGNC | HGNC:169. ACTR2. |
| HPA | CAB005083. HPA015050. |
| MIM | 604221. gene. |
| PharmGKB | PA24488. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | P61160. |
Gene expression databases | |
| ArrayExpress | P61160. |
| Bgee | P61160. |
| CleanEx | HS_ACTR2. |
| Genevestigator | P61160. |
| GermOnline | ENSG00000138071. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR004000. Actin-like. [Graphical view] |
| PANTHER | PTHR11937. Actin_like. 1 hit. |
| Pfam | PF00022. Actin. 1 hit. [Graphical view] |
| PRINTS | PR00190. ACTIN. |
| SMART | SM00268. ACTIN. 1 hit. [Graphical view] |
| PROSITE | PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 38189. |
| SOURCE | Search... |
Entry information
| Entry name | ARP2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P61160 Secondary accession number(s): B2RCP5, O15142, Q96C82 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


