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P61157

- ARP3_BOVIN

UniProt

P61157 - ARP3_BOVIN

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Protein

Actin-related protein 3

Gene
ACTR3, ARP3
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis By similarity.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct

GO - Biological processi

  1. asymmetric cell division Source: Ensembl
  2. cilium morphogenesis Source: UniProtKB
  3. establishment or maintenance of cell polarity Source: Ensembl
  4. meiotic cytokinesis Source: Ensembl
  5. spindle localization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.
REACT_211475. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 3
Alternative name(s):
Actin-2
Actin-like protein 3
Gene namesi
Name:ACTR3
Synonyms:ARP3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasmcytoskeleton By similarity. Cell projection By similarity
Note: In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) By similarity. Cell projection By similarity.

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-SubCell
  2. cytosol Source: Reactome
  3. lamellipodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 418417Actin-related protein 3PRO_0000089078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei240 – 2401N6-acetyllysine By similarity
Modified residuei244 – 2441N6-acetyllysine By similarity
Modified residuei251 – 2511N6-acetyllysine By similarity
Modified residuei254 – 2541N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61157.
PRIDEiP61157.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Interacts weakly with MEFV By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CttnQ605984EBI-351419,EBI-397955From a different organism.
PTK2Q009445EBI-351419,EBI-2896409From a different organism.
WASLO004013EBI-351419,EBI-957615From a different organism.

Protein-protein interaction databases

DIPiDIP-29790N.
IntActiP61157. 5 interactions.
STRINGi9913.ENSBTAP00000004410.

Structurei

Secondary structure

1
418
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 125
Beta strandi14 – 218
Beta strandi28 – 336
Beta strandi35 – 373
Helixi56 – 583
Beta strandi60 – 623
Helixi63 – 664
Beta strandi72 – 754
Turni77 – 804
Beta strandi82 – 843
Helixi86 – 9813
Turni99 – 1013
Helixi105 – 1073
Beta strandi110 – 1145
Helixi120 – 13213
Beta strandi137 – 1437
Helixi144 – 1518
Helixi152 – 1543
Beta strandi155 – 1573
Beta strandi165 – 1739
Beta strandi175 – 1817
Helixi187 – 1893
Beta strandi191 – 1944
Helixi197 – 20913
Helixi217 – 2193
Helixi220 – 23112
Helixi238 – 24710
Helixi249 – 2513
Beta strandi254 – 2596
Turni261 – 2633
Beta strandi266 – 2716
Helixi274 – 2807
Turni281 – 2833
Helixi285 – 2873
Helixi296 – 30611
Helixi309 – 3113
Helixi314 – 3174
Beta strandi319 – 3235
Helixi324 – 3263
Helixi331 – 35323
Turni372 – 3754
Helixi376 – 3849
Helixi388 – 3936
Beta strandi394 – 3963
Helixi397 – 4037
Helixi405 – 4095

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K8KX-ray2.00A1-418[»]
1TYQX-ray2.55A1-418[»]
1U2VX-ray2.55A1-418[»]
2P9IX-ray2.46A1-418[»]
2P9KX-ray2.59A1-418[»]
2P9LX-ray2.65A1-418[»]
2P9NX-ray2.85A1-418[»]
2P9PX-ray2.90A1-418[»]
2P9SX-ray2.68A1-418[»]
2P9UX-ray2.75A1-418[»]
3DXKX-ray2.70A1-418[»]
3DXMX-ray2.85A1-418[»]
3RSEX-ray2.65A1-418[»]
3UKRX-ray2.48A1-418[»]
3UKUX-ray2.75A1-418[»]
3ULEX-ray2.50A1-418[»]
4JD2X-ray3.08A1-418[»]
ProteinModelPortaliP61157.
SMRiP61157. Positions 3-417.

Miscellaneous databases

EvolutionaryTraceiP61157.

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP3 subfamily.

Phylogenomic databases

eggNOGiCOG5277.
HOGENOMiHOG000233339.
HOVERGENiHBG003771.
InParanoidiP61157.

Family and domain databases

InterProiIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61157-1 [UniParc]FASTAAdd to Basket

« Hide

MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR    50
VMKGVDDLDF FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY 100
LRAEPEDHYF LLTEPPLNTP ENREYTAEIM FESFNVPGLY IAVQAVLALA 150
ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV AEGYVIGSCI KHIPIAGRDI 200
TYFIQQLLRD REVGIPPEQS LETAKAVKER YSYVCPDLVK EFNKYDTDGS 250
KWIKQYTGIN AISKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV 300
DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS 350
EELSGGRLKP KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY 400
EEIGPSICRH NPVFGVMS 418
Length:418
Mass (Da):47,371
Last modified:January 23, 2007 - v3
Checksum:i23E5564198B81C63
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti344 – 3441D → G in AAI23583. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D12816 mRNA. Translation: BAA02249.1.
BC123582 mRNA. Translation: AAI23583.1.
BC142009 mRNA. Translation: AAI42010.1.
PIRiJQ1616.
RefSeqiNP_776651.1. NM_174226.2.
UniGeneiBt.4292.

Genome annotation databases

GeneIDi281597.
KEGGibta:281597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D12816 mRNA. Translation: BAA02249.1 .
BC123582 mRNA. Translation: AAI23583.1 .
BC142009 mRNA. Translation: AAI42010.1 .
PIRi JQ1616.
RefSeqi NP_776651.1. NM_174226.2.
UniGenei Bt.4292.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K8K X-ray 2.00 A 1-418 [» ]
1TYQ X-ray 2.55 A 1-418 [» ]
1U2V X-ray 2.55 A 1-418 [» ]
2P9I X-ray 2.46 A 1-418 [» ]
2P9K X-ray 2.59 A 1-418 [» ]
2P9L X-ray 2.65 A 1-418 [» ]
2P9N X-ray 2.85 A 1-418 [» ]
2P9P X-ray 2.90 A 1-418 [» ]
2P9S X-ray 2.68 A 1-418 [» ]
2P9U X-ray 2.75 A 1-418 [» ]
3DXK X-ray 2.70 A 1-418 [» ]
3DXM X-ray 2.85 A 1-418 [» ]
3RSE X-ray 2.65 A 1-418 [» ]
3UKR X-ray 2.48 A 1-418 [» ]
3UKU X-ray 2.75 A 1-418 [» ]
3ULE X-ray 2.50 A 1-418 [» ]
4JD2 X-ray 3.08 A 1-418 [» ]
ProteinModelPortali P61157.
SMRi P61157. Positions 3-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29790N.
IntActi P61157. 5 interactions.
STRINGi 9913.ENSBTAP00000004410.

Proteomic databases

PaxDbi P61157.
PRIDEi P61157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281597.
KEGGi bta:281597.

Organism-specific databases

CTDi 10096.

Phylogenomic databases

eggNOGi COG5277.
HOGENOMi HOG000233339.
HOVERGENi HBG003771.
InParanoidi P61157.

Enzyme and pathway databases

Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.
REACT_211475. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

EvolutionaryTracei P61157.
NextBioi 20805540.

Family and domain databases

InterProi IPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
[Graphical view ]
PANTHERi PTHR11937. PTHR11937. 1 hit.
Pfami PF00022. Actin. 1 hit.
[Graphical view ]
SMARTi SM00268. ACTIN. 1 hit.
[Graphical view ]
PROSITEi PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thymus.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF ARP2/3 COMPLEX.
  4. "Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP."
    Nolen B.J., Littlefield R.S., Pollard T.D.
    Proc. Natl. Acad. Sci. U.S.A. 101:15627-15632(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF ARP2/3 COMPLEX WITH BOUND ATP.

Entry informationi

Entry nameiARP3_BOVIN
AccessioniPrimary (citable) accession number: P61157
Secondary accession number(s): A5PJ90, P32391, Q08DS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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