Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P61157

- ARP3_BOVIN

UniProt

P61157 - ARP3_BOVIN

Protein

Actin-related protein 3

Gene

ACTR3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. asymmetric cell division Source: Ensembl
    2. cilium morphogenesis Source: UniProtKB
    3. establishment or maintenance of cell polarity Source: Ensembl
    4. meiotic cytokinesis Source: Ensembl
    5. spindle localization Source: Ensembl

    Keywords - Biological processi

    Cilium biogenesis/degradation

    Keywords - Ligandi

    Actin-binding, ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.
    REACT_211475. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin-related protein 3
    Alternative name(s):
    Actin-2
    Actin-like protein 3
    Gene namesi
    Name:ACTR3
    Synonyms:ARP3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Cell projection By similarity
    Note: In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes). Cell projection.By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. cytosol Source: Reactome
    3. lamellipodium Source: Ensembl

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 418417Actin-related protein 3PRO_0000089078Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei240 – 2401N6-acetyllysineBy similarity
    Modified residuei244 – 2441N6-acetyllysineBy similarity
    Modified residuei251 – 2511N6-acetyllysineBy similarity
    Modified residuei254 – 2541N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP61157.
    PRIDEiP61157.

    Interactioni

    Subunit structurei

    Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Interacts weakly with MEFV By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CttnQ605984EBI-351419,EBI-397955From a different organism.
    PTK2Q009445EBI-351419,EBI-2896409From a different organism.
    WASLO004013EBI-351419,EBI-957615From a different organism.

    Protein-protein interaction databases

    DIPiDIP-29790N.
    IntActiP61157. 5 interactions.
    STRINGi9913.ENSBTAP00000004410.

    Structurei

    Secondary structure

    1
    418
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 125
    Beta strandi14 – 218
    Beta strandi28 – 336
    Beta strandi35 – 373
    Helixi56 – 583
    Beta strandi60 – 623
    Helixi63 – 664
    Beta strandi72 – 754
    Turni77 – 804
    Beta strandi82 – 843
    Helixi86 – 9813
    Turni99 – 1013
    Helixi105 – 1073
    Beta strandi110 – 1145
    Helixi120 – 13213
    Beta strandi137 – 1437
    Helixi144 – 1518
    Helixi152 – 1543
    Beta strandi155 – 1573
    Beta strandi165 – 1739
    Beta strandi175 – 1817
    Helixi187 – 1893
    Beta strandi191 – 1944
    Helixi197 – 20913
    Helixi217 – 2193
    Helixi220 – 23112
    Helixi238 – 24710
    Helixi249 – 2513
    Beta strandi254 – 2596
    Turni261 – 2633
    Beta strandi266 – 2716
    Helixi274 – 2807
    Turni281 – 2833
    Helixi285 – 2873
    Helixi296 – 30611
    Helixi309 – 3113
    Helixi314 – 3174
    Beta strandi319 – 3235
    Helixi324 – 3263
    Helixi331 – 35323
    Turni372 – 3754
    Helixi376 – 3849
    Helixi388 – 3936
    Beta strandi394 – 3963
    Helixi397 – 4037
    Helixi405 – 4095

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K8KX-ray2.00A1-418[»]
    1TYQX-ray2.55A1-418[»]
    1U2VX-ray2.55A1-418[»]
    2P9IX-ray2.46A1-418[»]
    2P9KX-ray2.59A1-418[»]
    2P9LX-ray2.65A1-418[»]
    2P9NX-ray2.85A1-418[»]
    2P9PX-ray2.90A1-418[»]
    2P9SX-ray2.68A1-418[»]
    2P9UX-ray2.75A1-418[»]
    3DXKX-ray2.70A1-418[»]
    3DXMX-ray2.85A1-418[»]
    3RSEX-ray2.65A1-418[»]
    3UKRX-ray2.48A1-418[»]
    3UKUX-ray2.75A1-418[»]
    3ULEX-ray2.50A1-418[»]
    4JD2X-ray3.08A1-418[»]
    ProteinModelPortaliP61157.
    SMRiP61157. Positions 3-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP61157.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the actin family. ARP3 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5277.
    HOGENOMiHOG000233339.
    HOVERGENiHBG003771.
    InParanoidiP61157.

    Family and domain databases

    InterProiIPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    [Graphical view]
    PANTHERiPTHR11937. PTHR11937. 1 hit.
    PfamiPF00022. Actin. 1 hit.
    [Graphical view]
    SMARTiSM00268. ACTIN. 1 hit.
    [Graphical view]
    PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR    50
    VMKGVDDLDF FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY 100
    LRAEPEDHYF LLTEPPLNTP ENREYTAEIM FESFNVPGLY IAVQAVLALA 150
    ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV AEGYVIGSCI KHIPIAGRDI 200
    TYFIQQLLRD REVGIPPEQS LETAKAVKER YSYVCPDLVK EFNKYDTDGS 250
    KWIKQYTGIN AISKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV 300
    DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS 350
    EELSGGRLKP KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY 400
    EEIGPSICRH NPVFGVMS 418
    Length:418
    Mass (Da):47,371
    Last modified:January 23, 2007 - v3
    Checksum:i23E5564198B81C63
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti344 – 3441D → G in AAI23583. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12816 mRNA. Translation: BAA02249.1.
    BC123582 mRNA. Translation: AAI23583.1.
    BC142009 mRNA. Translation: AAI42010.1.
    PIRiJQ1616.
    RefSeqiNP_776651.1. NM_174226.2.
    UniGeneiBt.4292.

    Genome annotation databases

    GeneIDi281597.
    KEGGibta:281597.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12816 mRNA. Translation: BAA02249.1 .
    BC123582 mRNA. Translation: AAI23583.1 .
    BC142009 mRNA. Translation: AAI42010.1 .
    PIRi JQ1616.
    RefSeqi NP_776651.1. NM_174226.2.
    UniGenei Bt.4292.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K8K X-ray 2.00 A 1-418 [» ]
    1TYQ X-ray 2.55 A 1-418 [» ]
    1U2V X-ray 2.55 A 1-418 [» ]
    2P9I X-ray 2.46 A 1-418 [» ]
    2P9K X-ray 2.59 A 1-418 [» ]
    2P9L X-ray 2.65 A 1-418 [» ]
    2P9N X-ray 2.85 A 1-418 [» ]
    2P9P X-ray 2.90 A 1-418 [» ]
    2P9S X-ray 2.68 A 1-418 [» ]
    2P9U X-ray 2.75 A 1-418 [» ]
    3DXK X-ray 2.70 A 1-418 [» ]
    3DXM X-ray 2.85 A 1-418 [» ]
    3RSE X-ray 2.65 A 1-418 [» ]
    3UKR X-ray 2.48 A 1-418 [» ]
    3UKU X-ray 2.75 A 1-418 [» ]
    3ULE X-ray 2.50 A 1-418 [» ]
    4JD2 X-ray 3.08 A 1-418 [» ]
    ProteinModelPortali P61157.
    SMRi P61157. Positions 3-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29790N.
    IntActi P61157. 5 interactions.
    STRINGi 9913.ENSBTAP00000004410.

    Proteomic databases

    PaxDbi P61157.
    PRIDEi P61157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281597.
    KEGGi bta:281597.

    Organism-specific databases

    CTDi 10096.

    Phylogenomic databases

    eggNOGi COG5277.
    HOGENOMi HOG000233339.
    HOVERGENi HBG003771.
    InParanoidi P61157.

    Enzyme and pathway databases

    Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.
    REACT_211475. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    EvolutionaryTracei P61157.
    NextBioi 20805540.

    Family and domain databases

    InterProi IPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    [Graphical view ]
    PANTHERi PTHR11937. PTHR11937. 1 hit.
    Pfami PF00022. Actin. 1 hit.
    [Graphical view ]
    SMARTi SM00268. ACTIN. 1 hit.
    [Graphical view ]
    PROSITEi PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Thymus.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Thymus.
    3. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF ARP2/3 COMPLEX.
    4. "Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP."
      Nolen B.J., Littlefield R.S., Pollard T.D.
      Proc. Natl. Acad. Sci. U.S.A. 101:15627-15632(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF ARP2/3 COMPLEX WITH BOUND ATP.

    Entry informationi

    Entry nameiARP3_BOVIN
    AccessioniPrimary (citable) accession number: P61157
    Secondary accession number(s): A5PJ90, P32391, Q08DS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3