Reviewed, UniProtKB/Swiss-Prot P61154 (FWDB_METJA)

Names and origin

Protein names

Recommended name:
    Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B
    EC=1.2.99.5
Alternative name(s):
    Tungsten-containing formylmethanofuran dehydrogenase II subunit B

Gene names

Name:	fwdB
Ordered Locus Names:	MJ1194

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile By similarity.
Catalytic activity	Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.
Cofactor	Tungsten By similarity.
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3.
Subunit structure	This enzyme is composed of six subunits fwdA, fwdC, fwdD, fwdE, fwdF and fwdG By similarity.
Sequence similarities	Belongs to the fwdB family.

Ontologies

Keywords
Biological process	Methanogenesis
Coding sequence diversity	Selenocysteine
Ligand	Selenium Tungsten
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B		PRO_0000087392
Amino acid modifications
	Non-standard residue	121	1	Selenocysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	P61154-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: C1652D23FFC20970	FASTA	435	48,718
	10 20 30 40 50 60 MVKVVRNVVC PFCGTLCDDL EILVEDNHIV GTRHACRIGN AKFMHFEGAV RYTEPLMREN 70 80 90 100 110 120 KKDDFKKVDY ETAIEETARL LTEATLPLIY GWSATECHAH MYGVELAELV GAVIDNTASV 130 140 150 160 170 180 UHGPSLLAVQ DVGYPVCTLG EVKNRADVII FWGSNPMHAH PRHMSRYSVF ARGFFRERGR 190 200 210 220 230 240 EDRTLIVVDP RETDTAKLAD IHLQVEPHKD YELVSAMRAV LKGFELQVDK VAGVPADLIY 250 260 270 280 290 300 EAVEVCKNAQ FGELFFAMGV TMTRGKHRNI DNAIQLVIDL NAYTKFGLMP MRGHYNVNGF 310 320 330 340 350 360 NQVLTWVTGY PFGVDFSRGY PRYNPGETTA NDLLQRGETD MMLNIASDPG AHFPQKAVQH 370 380 390 400 410 420 MAKIPLVCID PHETPTTQLA NIIIPPAIAG VEVEGTAYRM DGVPIQLRKV IDPPEGVLPD 430 REILKILIKK VKEML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion." Wilting R., Schorling S., Persson B.C., Boeck A. J. Mol. Biol. 266:637-641(1997) [PubMed: 9102456] [Abstract] Cited for: PROBABLE SELENOCYSTEINE AT SEC-121.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus MJ1194 in contig L77117_GR.
TIGR	MJ1194.
Phylogenomic databases
HOGENOM	P61154.
OMA	P61154. NVVCPFC.
Enzyme and pathway databases
BRENDA	1.2.99.5. 256362.
Family and domain databases
InterPro	IPR016457. Formylmethanofuran_DH_bsu. IPR006656. Mopterin_OxRdtase. [Graphical view]
Pfam	PF00384. Molybdopterin. 1 hit. [Graphical view]
PIRSF	PIRSF005646. FwdB. 1 hit.
TIGRFAMs	TIGR03129. one_C_dehyd_B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FWDB_METJA

Accession

Primary (citable) accession number: P61154

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)