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Protein

Fibroblast growth factor 1

Gene

Fgf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331HeparinBy similarity

GO - Molecular functioni

  • fibroblast growth factor receptor binding Source: UniProtKB
  • growth factor activity Source: RGD
  • heparin binding Source: UniProtKB
  • Hsp70 protein binding Source: RGD
  • integrin binding Source: UniProtKB
  • S100 protein binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-RNO-109704. PI3K Cascade.
R-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-190322. FGFR4 ligand binding and activation.
R-RNO-190370. FGFR1b ligand binding and activation.
R-RNO-190371. FGFR3b ligand binding and activation.
R-RNO-190372. FGFR3c ligand binding and activation.
R-RNO-190373. FGFR1c ligand binding and activation.
R-RNO-190375. FGFR2c ligand binding and activation.
R-RNO-190377. FGFR2b ligand binding and activation.
R-RNO-5654219. Phospholipase C-mediated cascade: FGFR1.
R-RNO-5654221. Phospholipase C-mediated cascade, FGFR2.
R-RNO-5654227. Phospholipase C-mediated cascade, FGFR3.
R-RNO-5654228. Phospholipase C-mediated cascade, FGFR4.
R-RNO-5654687. Downstream signaling of activated FGFR1.
R-RNO-5654688. SHC-mediated cascade:FGFR1.
R-RNO-5654689. PI-3K cascade:FGFR1.
R-RNO-5654693. FRS-mediated FGFR1 signaling.
R-RNO-5654695. PI-3K cascade:FGFR2.
R-RNO-5654699. SHC-mediated cascade:FGFR2.
R-RNO-5654700. FRS-mediated FGFR2 signaling.
R-RNO-5654704. SHC-mediated cascade:FGFR3.
R-RNO-5654706. FRS-mediated FGFR3 signaling.
R-RNO-5654710. PI-3K cascade:FGFR3.
R-RNO-5654712. FRS-mediated FGFR4 signaling.
R-RNO-5654719. SHC-mediated cascade:FGFR4.
R-RNO-5654720. PI-3K cascade:FGFR4.
R-RNO-5654726. Negative regulation of FGFR1 signaling.
R-RNO-5654727. Negative regulation of FGFR2 signaling.
R-RNO-5654732. Negative regulation of FGFR3 signaling.
R-RNO-5654733. Negative regulation of FGFR4 signaling.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibroblast growth factor 1
Short name:
FGF-1
Alternative name(s):
Acidic fibroblast growth factor
Short name:
aFGF
Heparin-binding growth factor 1
Short name:
HBGF-1
Gene namesi
Name:Fgf1
Synonyms:Fgf-1, Fgfa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi2605. Fgf1.

Subcellular locationi

  • Secreted 1 Publication
  • Cytoplasm By similarity
  • Cytoplasmcell cortex By similarity
  • Cytoplasmcytosol By similarity
  • Nucleus By similarity

  • Note: Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu2+ ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity).By similarity

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: RGD
  • cytosol Source: UniProtKB
  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB
  • nucleolus Source: RGD
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
  • proteinaceous extracellular matrix Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Propeptidei2 – 1514By similarityPRO_0000008916Add
BLAST
Chaini16 – 155140Fibroblast growth factor 1PRO_0000008917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Post-translational modificationi

In the nucleus, phosphorylated by PKC/PRKCD.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP61149.
PRIDEiP61149.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013867.
GenevisibleiP61149. RN.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu2+-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13 (By similarity). Interacts with LRRC59 (By similarity). Interacts with CSNKA, CSNKB and FIBP (By similarity). While binding with LRRC59, CSNKA and FIBP seem mutually exclusive, CSNKB and FIBP may cooperatively interact with FGF1. Forms a ternary complex with FGFR1 and ITGAV:ITGB3 and induces the recruitment of PTPN11 to the complex (By similarity).By similarity

GO - Molecular functioni

  • fibroblast growth factor receptor binding Source: UniProtKB
  • growth factor activity Source: RGD
  • Hsp70 protein binding Source: RGD
  • integrin binding Source: UniProtKB
  • S100 protein binding Source: UniProtKB

Protein-protein interaction databases

IntActiP61149. 2 interactions.
MINTiMINT-2635533.
STRINGi10116.ENSRNOP00000018577.

Structurei

Secondary structure

1
155
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 315Combined sources
Turni32 – 354Combined sources
Beta strandi36 – 405Combined sources
Beta strandi44 – 507Combined sources
Helixi55 – 573Combined sources
Beta strandi59 – 657Combined sources
Beta strandi68 – 736Combined sources
Turni74 – 763Combined sources
Beta strandi79 – 824Combined sources
Beta strandi88 – 936Combined sources
Helixi96 – 983Combined sources
Beta strandi100 – 1045Combined sources
Beta strandi110 – 1156Combined sources
Helixi118 – 1203Combined sources
Beta strandi130 – 1323Combined sources
Helixi135 – 1373Combined sources
Helixi143 – 1453Combined sources
Beta strandi147 – 1515Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J3PX-ray1.40A/B22-155[»]
2UUSX-ray2.20A/B22-153[»]
ProteinModelPortaliP61149.
SMRiP61149. Positions 22-155.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61149.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni127 – 14317Heparin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3885. Eukaryota.
ENOG4111IPH. LUCA.
GeneTreeiENSGT00730000110923.
HOGENOMiHOG000236341.
HOVERGENiHBG007580.
InParanoidiP61149.
KOiK18496.
OMAiLGPRTHY.
OrthoDBiEOG091G0NAY.
PhylomeDBiP61149.
TreeFamiTF317805.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR028210. FGF1.
IPR002209. Fibroblast_GF_fam.
IPR028142. IL-1_fam/FGF_fam.
[Graphical view]
PANTHERiPTHR11486. PTHR11486. 1 hit.
PTHR11486:SF86. PTHR11486:SF86. 1 hit.
PfamiPF00167. FGF. 1 hit.
[Graphical view]
PRINTSiPR00263. HBGFFGF.
PR00262. IL1HBGF.
SMARTiSM00442. FGF. 1 hit.
[Graphical view]
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00247. HBGF_FGF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR
60 70 80 90 100
DRSDQHIQLQ LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF
110 120 130 140 150
LERLEENHYN TYTSKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL

PVSSD
Length:155
Mass (Da):17,418
Last modified:May 10, 2004 - v1
Checksum:i8880E4FF0FBA4161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14232 mRNA. Translation: CAA32448.1.
PIRiS04147.
RefSeqiNP_036978.1. NM_012846.1.
XP_006254712.1. XM_006254650.2.
XP_006254713.1. XM_006254651.2.
XP_006254714.1. XM_006254652.2.
XP_006254715.1. XM_006254653.2.
UniGeneiRn.88013.

Genome annotation databases

EnsembliENSRNOT00000018577; ENSRNOP00000018577; ENSRNOG00000013867.
ENSRNOT00000087408; ENSRNOP00000070685; ENSRNOG00000013867.
GeneIDi25317.
KEGGirno:25317.
UCSCiRGD:2605. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14232 mRNA. Translation: CAA32448.1.
PIRiS04147.
RefSeqiNP_036978.1. NM_012846.1.
XP_006254712.1. XM_006254650.2.
XP_006254713.1. XM_006254651.2.
XP_006254714.1. XM_006254652.2.
XP_006254715.1. XM_006254653.2.
UniGeneiRn.88013.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J3PX-ray1.40A/B22-155[»]
2UUSX-ray2.20A/B22-153[»]
ProteinModelPortaliP61149.
SMRiP61149. Positions 22-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP61149. 2 interactions.
MINTiMINT-2635533.
STRINGi10116.ENSRNOP00000018577.

Proteomic databases

PaxDbiP61149.
PRIDEiP61149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018577; ENSRNOP00000018577; ENSRNOG00000013867.
ENSRNOT00000087408; ENSRNOP00000070685; ENSRNOG00000013867.
GeneIDi25317.
KEGGirno:25317.
UCSCiRGD:2605. rat.

Organism-specific databases

CTDi2246.
RGDi2605. Fgf1.

Phylogenomic databases

eggNOGiKOG3885. Eukaryota.
ENOG4111IPH. LUCA.
GeneTreeiENSGT00730000110923.
HOGENOMiHOG000236341.
HOVERGENiHBG007580.
InParanoidiP61149.
KOiK18496.
OMAiLGPRTHY.
OrthoDBiEOG091G0NAY.
PhylomeDBiP61149.
TreeFamiTF317805.

Enzyme and pathway databases

ReactomeiR-RNO-109704. PI3K Cascade.
R-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-190322. FGFR4 ligand binding and activation.
R-RNO-190370. FGFR1b ligand binding and activation.
R-RNO-190371. FGFR3b ligand binding and activation.
R-RNO-190372. FGFR3c ligand binding and activation.
R-RNO-190373. FGFR1c ligand binding and activation.
R-RNO-190375. FGFR2c ligand binding and activation.
R-RNO-190377. FGFR2b ligand binding and activation.
R-RNO-5654219. Phospholipase C-mediated cascade: FGFR1.
R-RNO-5654221. Phospholipase C-mediated cascade, FGFR2.
R-RNO-5654227. Phospholipase C-mediated cascade, FGFR3.
R-RNO-5654228. Phospholipase C-mediated cascade, FGFR4.
R-RNO-5654687. Downstream signaling of activated FGFR1.
R-RNO-5654688. SHC-mediated cascade:FGFR1.
R-RNO-5654689. PI-3K cascade:FGFR1.
R-RNO-5654693. FRS-mediated FGFR1 signaling.
R-RNO-5654695. PI-3K cascade:FGFR2.
R-RNO-5654699. SHC-mediated cascade:FGFR2.
R-RNO-5654700. FRS-mediated FGFR2 signaling.
R-RNO-5654704. SHC-mediated cascade:FGFR3.
R-RNO-5654706. FRS-mediated FGFR3 signaling.
R-RNO-5654710. PI-3K cascade:FGFR3.
R-RNO-5654712. FRS-mediated FGFR4 signaling.
R-RNO-5654719. SHC-mediated cascade:FGFR4.
R-RNO-5654720. PI-3K cascade:FGFR4.
R-RNO-5654726. Negative regulation of FGFR1 signaling.
R-RNO-5654727. Negative regulation of FGFR2 signaling.
R-RNO-5654732. Negative regulation of FGFR3 signaling.
R-RNO-5654733. Negative regulation of FGFR4 signaling.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

EvolutionaryTraceiP61149.
PROiP61149.

Gene expression databases

BgeeiENSRNOG00000013867.
GenevisibleiP61149. RN.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR028210. FGF1.
IPR002209. Fibroblast_GF_fam.
IPR028142. IL-1_fam/FGF_fam.
[Graphical view]
PANTHERiPTHR11486. PTHR11486. 1 hit.
PTHR11486:SF86. PTHR11486:SF86. 1 hit.
PfamiPF00167. FGF. 1 hit.
[Graphical view]
PRINTSiPR00263. HBGFFGF.
PR00262. IL1HBGF.
SMARTiSM00442. FGF. 1 hit.
[Graphical view]
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00247. HBGF_FGF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFGF1_RAT
AccessioniPrimary (citable) accession number: P61149
Secondary accession number(s): P10935
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.