P61148 (FGF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibroblast growth factor 1
Short name=FGF-1

Alternative name(s):
Acidic fibroblast growth factor
Short name=aFGF
Heparin-binding growth factor 1
Short name=HBGF-1

Gene names

Name:	Fgf1
Synonyms:	Fgf-1, Fgfa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro By similarity.
Subunit structure	Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu2+-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13 By similarity. Interacts with LRRC59 By similarity. Interacts with CSNKA, CSNKB and FIBP By similarity. While binding with LRRC59, CSNKA and FIBP seem mutually exclusive, CSNKB and FIBP may cooperatively interact with FGF1 By similarity.
Subcellular location	Secreted. Cytoplasm By similarity. Cytoplasm › cell cortex By similarity. Cytoplasm › cytosol By similarity. Nucleus By similarity. Note: Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu2+ ions and S100A13. Secreted in a complex with SYT1. Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 By similarity.
Post-translational modification	In the nucleus, phosphorylated by PKC/PRKCD By similarity.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Cytoplasm Nucleus Secreted
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW branch elongation involved in ureteric bud branching Inferred from sequence or structural similarity. Source: UniProtKB cardiac muscle cell proliferation Traceable author statement PubMed 20299672. Source: DFLAT cellular response to heat Inferred from sequence or structural similarity. Source: UniProtKB fibroblast growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB lung development Inferred from direct assay PubMed 15576401. Source: MGI mesonephric epithelium development Inferred from sequence or structural similarity. Source: UniProtKB organ induction Inferred from direct assay PubMed 15576401. Source: MGI positive regulation of angiogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell division Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cholesterol biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of epithelial cell proliferation Inferred from direct assay PubMed 17187775. Source: MGI positive regulation of intracellular protein kinase cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein phosphorylation Inferred from direct assay PubMed 15800000. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 8898217. Source: MGI vasculogenesis involved in coronary vascular morphogenesis Traceable author statement PubMed 20299672. Source: DFLAT
Cellular_component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from sequence or structural similarity. Source: UniProtKB extracellular space Inferred from direct assay PubMed 15576401. Source: MGI nucleolus Inferred from electronic annotation. Source: Compara proteinaceous extracellular matrix Inferred from direct assay PubMed 15381701. Source: MGI
Molecular_function	S100 protein binding Inferred from sequence or structural similarity. Source: UniProtKB fibroblast growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB growth factor activity Inferred from sequence or structural similarity. Source: UniProtKB heparin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Propeptide	2 – 15	14	By similarity		PRO_0000008911
	Chain	16 – 155	140	Fibroblast growth factor 1		PRO_0000008912
Regions
	Region	127 – 143	17	Heparin-binding By similarity
Sites
	Binding site	33	1	Heparin By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61148 [UniParc]. Last modified May 10, 2004. Version 1. Checksum: 8880E4FF0FBA4161	FASTA	155	17,418
	10 20 30 40 50 60 MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ 70 80 90 100 110 120 LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK 130 140 150 NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis." Hebert J.M., Basilico C., Goldfarb M., Haub O., Martin G.R. Dev. Biol. 138:454-463(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of the mouse Fgf-1 gene." Madiai F., Hackshaw K.V., Chiu I.M. Gene 179:231-236(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of the 1B promoter of fibroblast growth factor 1 and its expression in the adult and developing mouse brain." Alam K.Y., Frostholm A., Hackshaw K.V., Evans J.E., Rotter A., Chiu I.M. J. Biol. Chem. 271:30263-30271(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30641 mRNA. Translation: AAA37618.1. U36459, U36457, U36458 Genomic DNA. Translation: AAC52969.1. U67610 mRNA. Translation: AAC52907.1. BC037601 mRNA. Translation: AAH37601.1.
IPI	IPI00115244.
PIR	D37360.
RefSeq	NP_034327.1. NM_010197.3.
UniGene	Mm.241282.
3D structure databases
ProteinModelPortal	P61148.
SMR	P61148. Positions 22-155.
ModBase	Search...
Protein family/group databases
TCDB	9.A.48.1.1. unconventional protein secretion (UPS) system.
Proteomic databases
PaxDb	P61148.
PRIDE	P61148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000040647; ENSMUSP00000045710; ENSMUSG00000036585. ENSMUST00000115582; ENSMUSP00000111245; ENSMUSG00000036585.
GeneID	14164.
KEGG	mmu:14164.
UCSC	uc008esn.1. mouse.
Organism-specific databases
CTD	2246.
MGI	MGI:95515. Fgf1.
Phylogenomic databases
eggNOG	NOG246422.
HOGENOM	HOG000236341.
HOVERGEN	HBG007580.
InParanoid	P61148.
KO	K04358.
OMA	PSEECLF.
OrthoDB	EOG48KRCM.
Gene expression databases
ArrayExpress	P61148.
Bgee	P61148.
CleanEx	MM_FGF1.
Genevestigator	P61148.
GermOnline	ENSMUSG00000036585. Mus musculus.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002209. GF_heparin-bd. IPR002348. IL1_HBGF. [Graphical view]
PANTHER	PTHR11486. PTHR11486. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
PRINTS	PR00263. HBGFFGF. PR00262. IL1HBGF.
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FGF1. mouse.
NextBio	285302.
SOURCE	Search...

Entry information

Entry name

FGF1_MOUSE

Accession

Primary (citable) accession number: P61148
Secondary accession number(s): P10935

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	May 29, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program