P61107 (RAB14_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-14

Gene names

Name:

Rab14

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates, together with its guanine nucleotide exchange factor, DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion By similarity. Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes. Ref.2
Subunit structure	Interacts with KIF16B. Interacts with ZFYVE20 By similarity.
Subcellular location	Recycling endosome By similarity. Early endosome membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus › trans-Golgi network membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Recruited to recycling endosomes by DENND6A By similarity. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Ref.2
Tissue specificity	Widely expressed (at protein level). Highest levels found in whole brain, spinal cord, heart, kidney and lung.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Endosome Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from sequence or structural similarity. Source: UniProtKB apical protein localization Inferred from direct assay PubMed 18429929. Source: RGD body fluid secretion Inferred from mutant phenotype PubMed 18332131. Source: RGD embryo development Inferred from sequence or structural similarity. Source: UniProtKB endocytic recycling Inferred from sequence or structural similarity. Source: UniProtKB fibroblast growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB neurotransmitter secretion Non-traceable author statement. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of protein localization Inferred from direct assay PubMed 18429929. Source: RGD small GTPase mediated signal transduction Non-traceable author statement. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from direct assay Ref.2. Source: UniProtKB alveolar lamellar body Inferred from direct assay PubMed 18332131. Source: RGD apical plasma membrane Inferred from direct assay PubMed 18429929. Source: RGD cytosol Inferred from direct assay Ref.2. Source: UniProtKB early endosome Inferred from direct assay Ref.2. Source: UniProtKB early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from direct assay Ref.2. Source: UniProtKB lysosome Inferred from direct assay Ref.2. Source: UniProtKB nuclear outer membrane-endoplasmic reticulum membrane network Inferred from direct assay Ref.2. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay Ref.2. Source: UniProtKB phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell recycling endosome Inferred from sequence or structural similarity. Source: UniProtKB rough endoplasmic reticulum Inferred from direct assay Ref.2. Source: UniProtKB trans-Golgi network transport vesicle Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB glycoprotein binding Inferred from direct assay PubMed 18429929. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 215	214	Ras-related protein Rab-14		PRO_0000121187
Regions
	Nucleotide binding	18 – 26	9	GTP Probable
	Nucleotide binding	66 – 70	5	GTP Probable
	Nucleotide binding	124 – 127	4	GTP Probable
	Nucleotide binding	154 – 156	3	GTP By similarity
	Motif	40 – 48	9	Effector region By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	98	1	Phosphothreonine By similarity
	Modified residue	99	1	Phosphotyrosine By similarity
	Modified residue	215	1	Cysteine methyl ester By similarity
	Lipidation	213	1	S-geranylgeranyl cysteine By similarity
	Lipidation	215	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	25	1	S → N: Mimicks the GTP-bound state and is enriched in the Golgi region. Ref.2
	Mutagenesis	70	1	Q → L: Mimicks the GDP-bound state. Ref.2
	Mutagenesis	124	1	N → I: Enriched in the Golgi region. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P61107 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B435AAC6F562B007	FASTA	215	23,927
	10 20 30 40 50 60 MATTPYNYSY IFKYIIIGDM GVGKSCLLHQ FTEKKFMADC PHTIGVEFGT RIIEVSGQKI 70 80 90 100 110 120 KLQIWDTAGQ ERFRAVTRSY YRGAAGALMV YDITRRSTYN HLSSWLTDAR NLTNPNTVII 130 140 150 160 170 180 LIGNKADLEA QRDVTYEEAK QFAEENGLLF LEASAKTGEN VEDAFLEAAK KIYQNIQDGS 190 200 210 LDLNAAESGV QHKPSAPQGG RLTSEPQPQR EGCGC

References

[1]	"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain." Elferink L.A., Anzai K., Scheller R.H. J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Rab14 is involved in membrane trafficking between the Golgi complex and endosomes." Junutula J.R., De Maziere A.M., Peden A.A., Ervin K.E., Advani R.J., van Dijk S.M., Klumperman J., Scheller R.H. Mol. Biol. Cell 15:2218-2229(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-25; GLN-70 AND ASN-124.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83680 mRNA. Translation: AAA41994.1.
IPI	IPI00196794.
PIR	E42148.
RefSeq	NP_446041.1. NM_053589.1.
UniGene	Rn.6008.
3D structure databases
ProteinModelPortal	P61107.
SMR	P61107. Positions 7-173.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61107. 1 interaction.
STRING	10116.ENSRNOP00000025649.
PTM databases
PhosphoSite	P61107.
Proteomic databases
PaxDb	P61107.
PRIDE	P61107.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	94197.
KEGG	rno:94197.
UCSC	RGD:620881. rat.
Organism-specific databases
CTD	51552.
RGD	620881. Rab14.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	P61107.
KO	K07881.
OrthoDB	EOG4MGS89.
Enzyme and pathway databases
Reactome	REACT_97881. Membrane Trafficking.
Gene expression databases
ArrayExpress	P61107.
Genevestigator	P61107.
GermOnline	ENSRNOG00000018901. Rattus norvegicus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	55334.

Entry information

Entry name

RAB14_RAT

Accession

Primary (citable) accession number: P61107
Secondary accession number(s): P35287, Q969L0, Q9UI11

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program