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Protein

Ras-related protein Rab-14

Gene

Rab14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates, together with its guanine nucleotide exchange factor, DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion (By similarity). Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269GTPCurated
Nucleotide bindingi66 – 705GTPCurated
Nucleotide bindingi124 – 1274GTPCurated
Nucleotide bindingi154 – 1563GTPBy similarity

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • glycoprotein binding Source: RGD
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

  • apical protein localization Source: RGD
  • body fluid secretion Source: RGD
  • defense response to bacterium Source: InterPro
  • embryo development Source: UniProtKB
  • endocytic recycling Source: UniProtKB
  • fibroblast growth factor receptor signaling pathway Source: UniProtKB
  • Golgi to endosome transport Source: UniProtKB
  • intracellular protein transport Source: GO_Central
  • intracellular transport Source: UniProtKB
  • metabolic process Source: GOC
  • phagolysosome assembly involved in apoptotic cell clearance Source: GO_Central
  • Rab protein signal transduction Source: GO_Central
  • regulation of protein localization Source: RGD
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_347820. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-14
Gene namesi
Name:Rab14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620881. Rab14.

Subcellular locationi

GO - Cellular componenti

  • alveolar lamellar body Source: RGD
  • apical plasma membrane Source: RGD
  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: UniProtKB
  • early endosome Source: UniProtKB
  • early endosome membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • Golgi stack Source: UniProtKB
  • late endosome Source: UniProtKB
  • lysosome Source: UniProtKB
  • nuclear outer membrane-endoplasmic reticulum membrane network Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • recycling endosome Source: UniProtKB
  • rough endoplasmic reticulum Source: UniProtKB
  • trans-Golgi network Source: InterPro
  • trans-Golgi network transport vesicle Source: UniProtKB
  • transport vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi25 – 251S → N: Mimicks the GTP-bound state and is enriched in the Golgi region. 1 Publication
Mutagenesisi70 – 701Q → L: Mimicks the GDP-bound state. 1 Publication
Mutagenesisi124 – 1241N → I: Enriched in the Golgi region. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 215214Ras-related protein Rab-14PRO_0000121187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Lipidationi213 – 2131S-geranylgeranyl cysteineBy similarity
Modified residuei215 – 2151Cysteine methyl esterBy similarity
Lipidationi215 – 2151S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP61107.
PRIDEiP61107.

PTM databases

PhosphoSiteiP61107.

Expressioni

Tissue specificityi

Widely expressed (at protein level). Highest levels found in whole brain, spinal cord, heart, kidney and lung.

Gene expression databases

GenevisibleiP61107. RN.

Interactioni

Subunit structurei

Interacts with KIF16B. Interacts with ZFYVE20 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NISCHQ9Y2I19EBI-917845,EBI-2688731From a different organism.

Protein-protein interaction databases

BioGridi250177. 3 interactions.
IntActiP61107. 6 interactions.
MINTiMINT-4542699.
STRINGi10116.ENSRNOP00000025649.

Structurei

3D structure databases

ProteinModelPortaliP61107.
SMRiP61107. Positions 7-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 489Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP61107.
KOiK07881.
PhylomeDBiP61107.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR030702. Rab14.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PANTHERiPTHR24073:SF185. PTHR24073:SF185. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTPYNYSY IFKYIIIGDM GVGKSCLLHQ FTEKKFMADC PHTIGVEFGT
60 70 80 90 100
RIIEVSGQKI KLQIWDTAGQ ERFRAVTRSY YRGAAGALMV YDITRRSTYN
110 120 130 140 150
HLSSWLTDAR NLTNPNTVII LIGNKADLEA QRDVTYEEAK QFAEENGLLF
160 170 180 190 200
LEASAKTGEN VEDAFLEAAK KIYQNIQDGS LDLNAAESGV QHKPSAPQGG
210
RLTSEPQPQR EGCGC
Length:215
Mass (Da):23,927
Last modified:January 23, 2007 - v3
Checksum:iB435AAC6F562B007
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83680 mRNA. Translation: AAA41994.1.
PIRiE42148.
RefSeqiNP_446041.1. NM_053589.1.
UniGeneiRn.163656.
Rn.6008.

Genome annotation databases

GeneIDi94197.
KEGGirno:94197.
UCSCiRGD:620881. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83680 mRNA. Translation: AAA41994.1.
PIRiE42148.
RefSeqiNP_446041.1. NM_053589.1.
UniGeneiRn.163656.
Rn.6008.

3D structure databases

ProteinModelPortaliP61107.
SMRiP61107. Positions 7-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250177. 3 interactions.
IntActiP61107. 6 interactions.
MINTiMINT-4542699.
STRINGi10116.ENSRNOP00000025649.

PTM databases

PhosphoSiteiP61107.

Proteomic databases

PaxDbiP61107.
PRIDEiP61107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi94197.
KEGGirno:94197.
UCSCiRGD:620881. rat.

Organism-specific databases

CTDi51552.
RGDi620881. Rab14.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP61107.
KOiK07881.
PhylomeDBiP61107.

Enzyme and pathway databases

ReactomeiREACT_347820. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi617858.
PROiP61107.

Gene expression databases

GenevisibleiP61107. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR030702. Rab14.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PANTHERiPTHR24073:SF185. PTHR24073:SF185. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
    Elferink L.A., Anzai K., Scheller R.H.
    J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Rab14 is involved in membrane trafficking between the Golgi complex and endosomes."
    Junutula J.R., De Maziere A.M., Peden A.A., Ervin K.E., Advani R.J., van Dijk S.M., Klumperman J., Scheller R.H.
    Mol. Biol. Cell 15:2218-2229(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-25; GLN-70 AND ASN-124.

Entry informationi

Entry nameiRAB14_RAT
AccessioniPrimary (citable) accession number: P61107
Secondary accession number(s): P35287, Q969L0, Q9UI11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.