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P61107 (RAB14_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-14
Gene names
Name:Rab14
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates, together with its guanine nucleotide exchange factor, DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion By similarity. Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes. Ref.2

Subunit structure

Interacts with KIF16B. Interacts with ZFYVE20 By similarity.

Subcellular location

Recycling endosome By similarity. Early endosome membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatustrans-Golgi network membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Recruited to recycling endosomes by DENND6A By similarity. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Ref.2

Tissue specificity

Widely expressed (at protein level). Highest levels found in whole brain, spinal cord, heart, kidney and lung.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasmic vesicle
Endosome
Golgi apparatus
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Lipoprotein
Methylation
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from sequence or structural similarity. Source: GOC

Golgi to endosome transport

Inferred from sequence or structural similarity. Source: UniProtKB

apical protein localization

Inferred from direct assay PubMed 18429929. Source: RGD

body fluid secretion

Inferred from mutant phenotype PubMed 18332131. Source: RGD

embryo development

Inferred from sequence or structural similarity. Source: UniProtKB

endocytic recycling

Inferred from sequence or structural similarity. Source: UniProtKB

fibroblast growth factor receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular transport

Non-traceable author statement Ref.2. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of protein localization

Inferred from direct assay PubMed 18429929. Source: RGD

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

vesicle-mediated transport

Non-traceable author statement Ref.2. Source: UniProtKB

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Golgi stack

Inferred from direct assay Ref.2. Source: UniProtKB

alveolar lamellar body

Inferred from direct assay PubMed 18332131. Source: RGD

apical plasma membrane

Inferred from direct assay PubMed 18429929. Source: RGD

cytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

cytosol

Inferred from direct assay Ref.2. Source: UniProtKB

early endosome

Inferred from direct assay Ref.2. Source: UniProtKB

early endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

late endosome

Inferred from direct assay Ref.2. Source: UniProtKB

lysosome

Inferred from direct assay Ref.2. Source: UniProtKB

nuclear outer membrane-endoplasmic reticulum membrane network

Inferred from direct assay Ref.2. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from direct assay Ref.2. Source: UniProtKB

phagocytic vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from direct assay Ref.2. Source: UniProtKB

recycling endosome

Inferred from sequence or structural similarity. Source: UniProtKB

rough endoplasmic reticulum

Inferred from direct assay Ref.2. Source: UniProtKB

trans-Golgi network transport vesicle

Inferred from direct assay Ref.2. Source: UniProtKB

transport vesicle

Inferred from direct assay PubMed 18429929. Source: RGD

   Molecular_functionGDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

glycoprotein binding

Inferred from direct assay PubMed 18429929. Source: RGD

protein binding

Inferred from physical interaction PubMed 18332131. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 215214Ras-related protein Rab-14
PRO_0000121187

Regions

Nucleotide binding18 – 269GTP Probable
Nucleotide binding66 – 705GTP Probable
Nucleotide binding124 – 1274GTP Probable
Nucleotide binding154 – 1563GTP By similarity
Motif40 – 489Effector region By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue2151Cysteine methyl ester By similarity
Lipidation2131S-geranylgeranyl cysteine By similarity
Lipidation2151S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis251S → N: Mimicks the GTP-bound state and is enriched in the Golgi region. Ref.2
Mutagenesis701Q → L: Mimicks the GDP-bound state. Ref.2
Mutagenesis1241N → I: Enriched in the Golgi region. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P61107 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B435AAC6F562B007

FASTA21523,927
        10         20         30         40         50         60 
MATTPYNYSY IFKYIIIGDM GVGKSCLLHQ FTEKKFMADC PHTIGVEFGT RIIEVSGQKI 

        70         80         90        100        110        120 
KLQIWDTAGQ ERFRAVTRSY YRGAAGALMV YDITRRSTYN HLSSWLTDAR NLTNPNTVII 

       130        140        150        160        170        180 
LIGNKADLEA QRDVTYEEAK QFAEENGLLF LEASAKTGEN VEDAFLEAAK KIYQNIQDGS 

       190        200        210 
LDLNAAESGV QHKPSAPQGG RLTSEPQPQR EGCGC 

« Hide

References

[1]"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
Elferink L.A., Anzai K., Scheller R.H.
J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Rab14 is involved in membrane trafficking between the Golgi complex and endosomes."
Junutula J.R., De Maziere A.M., Peden A.A., Ervin K.E., Advani R.J., van Dijk S.M., Klumperman J., Scheller R.H.
Mol. Biol. Cell 15:2218-2229(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-25; GLN-70 AND ASN-124.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M83680 mRNA. Translation: AAA41994.1.
PIRE42148.
RefSeqNP_446041.1. NM_053589.1.
UniGeneRn.163656.
Rn.6008.

3D structure databases

ProteinModelPortalP61107.
SMRP61107. Positions 7-173.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250177. 2 interactions.
IntActP61107. 3 interactions.
MINTMINT-4542699.
STRING10116.ENSRNOP00000025649.

PTM databases

PhosphoSiteP61107.

Proteomic databases

PaxDbP61107.
PRIDEP61107.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID94197.
KEGGrno:94197.
UCSCRGD:620881. rat.

Organism-specific databases

CTD51552.
RGD620881. Rab14.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP61107.
KOK07881.
PhylomeDBP61107.

Gene expression databases

GenevestigatorP61107.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio55334.
PROP61107.

Entry information

Entry nameRAB14_RAT
AccessionPrimary (citable) accession number: P61107
Secondary accession number(s): P35287, Q969L0, Q9UI11
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families