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Protein

Ubiquitin-conjugating enzyme E2 N

Gene

Ube2n

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains (PubMed:22424771, PubMed:28039360). This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.By similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation2 Publications

Enzyme regulationi

Activity is inhibited by binding to OTUB1, which prevents 'Lys-63'-linked polyubiquitination (By similarity). Activity is inhibited by GPS2, leading to prevent 'Lys-63'-linked polyubiquitination (PubMed:22424771, PubMed:28039360, PubMed:28123943, PubMed:29499132).By similarity4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1169408 ISG15 antiviral mechanism
R-MMU-168638 NOD1/2 Signaling Pathway
R-MMU-202424 Downstream TCR signaling
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-MMU-450302 activated TAK1 mediates p38 MAPK activation
R-MMU-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-69473 G2/M DNA damage checkpoint
R-MMU-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-MMU-9020702 Interleukin-1 signaling
R-MMU-937039 IRAK1 recruits IKK complex
R-MMU-937041 IKK complex recruitment mediated by RIP1
R-MMU-975110 TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling
R-MMU-975144 IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 N (EC:2.3.2.232 Publications)
Alternative name(s):
Bendless-like ubiquitin-conjugating enzyme
E2 ubiquitin-conjugating enzyme N
Ubc13
Ubiquitin carrier protein N
Ubiquitin-protein ligase N
Gene namesi
Name:Ube2n
Synonyms:Blu
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1934835 Ube2n

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825041 – 152Ubiquitin-conjugating enzyme E2 NAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82N6-acetyllysineBy similarity1
Cross-linki92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

Post-translational modificationi

Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP61089
MaxQBiP61089
PaxDbiP61089
PRIDEiP61089
TopDownProteomicsiP61089

2D gel databases

REPRODUCTION-2DPAGEiIPI00165854
P61089

PTM databases

iPTMnetiP61089
PhosphoSitePlusiP61089
SwissPalmiP61089

Expressioni

Gene expression databases

BgeeiENSMUSG00000074781
CleanExiMM_UBE2N
ExpressionAtlasiP61089 baseline and differential
GenevisibleiP61089 MM

Interactioni

Subunit structurei

Heterodimer with UBE2V2 (By similarity). Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity (By similarity). Interacts with RNF8 and RNF168 (By similarity). Interacts with RNF11 (By similarity). Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair (By similarity). Interacts with ARIH2 (via RING-type 2) (By similarity). Interacts with OTUB1; leading to inhibit E2-conjugating activity (By similarity). Interacts with GPS2; leading to inhibit E2-conjugating activity (PubMed:22424771).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220301, 21 interactors
CORUMiP61089
IntActiP61089, 8 interactors
MINTiP61089
STRINGi10090.ENSMUSP00000096932

Structurei

3D structure databases

ProteinModelPortaliP61089
SMRiP61089
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00540000070023
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiP61089
KOiK10580
OMAiIDKWSPA
OrthoDBiEOG091G0VPD
PhylomeDBiP61089
TreeFamiTF101126

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P61089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG
60 70 80 90 100
TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ
110 120 130 140 150
IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN

NI
Length:152
Mass (Da):17,138
Last modified:April 26, 2004 - v1
Checksum:iFACD84D883D77407
GO

Sequence cautioni

The sequence BAE36659 differs from that shown. Reason: Frameshift at position 105.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114A → D in BAB24239 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09873 mRNA Translation: CAA71001.1
AY039837 mRNA Translation: AAK74128.1
AK005302 mRNA Translation: BAB23941.1
AK005788 mRNA Translation: BAB24239.1
AK161968 mRNA Translation: BAE36659.1 Frameshift.
BC034898 mRNA Translation: AAH34898.3
BC067069 mRNA Translation: AAH67069.1
CCDSiCCDS48677.1
RefSeqiNP_542127.1, NM_080560.3
UniGeneiMm.371667
Mm.440187
Mm.486592

Genome annotation databases

EnsembliENSMUST00000099329; ENSMUSP00000096932; ENSMUSG00000074781
GeneIDi93765
KEGGimmu:93765
UCSCiuc007gwm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiUBE2N_MOUSE
AccessioniPrimary (citable) accession number: P61089
Secondary accession number(s): Q16781
, Q3TSL6, Q6ZWZ0, Q9DAJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: April 25, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health