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Protein

Ubiquitin-conjugating enzyme E2 K

Gene

Ube2k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. Involved in stabilization of CASP12 during ER stress-mediated beta-amyloid neurotoxicity probably by inhibiting proteasome activity; in vitro ubiquitinates CASP12.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 K (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme K
Huntingtin-interacting protein 2
Short name:
HIP-2
Ubiquitin carrier protein
Ubiquitin-conjugating enzyme E2-25 kDa
Short name:
Ubiquitin-conjugating enzyme E2(25K)
Short name:
Ubiquitin-conjugating enzyme E2-25K
Ubiquitin-protein ligase
Gene namesi
Name:Ube2k
Synonyms:Hip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1858216. Ube2k.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • filopodium tip Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Neurons are resistant to beta-amyloid neurotoxicity. Significantly lower CASP12 expression in brain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000824442 – 200Ubiquitin-conjugating enzyme E2 KAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei14N6-acetyllysine; alternateBy similarity1
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei159PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation at Lys-14 impairs catalytic activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP61087.
PaxDbiP61087.
PeptideAtlasiP61087.
PRIDEiP61087.

2D gel databases

REPRODUCTION-2DPAGEP61087.

PTM databases

iPTMnetiP61087.
PhosphoSitePlusiP61087.
SwissPalmiP61087.

Expressioni

Tissue specificityi

Expressed in the brain, with highest levels found in the mitral cells of the olfactory bulb, the pyramidal cell layer of the hippocampus and the Purkinje cells of the cerebellar cortex.1 Publication

Developmental stagei

Expressed at all stages of brain development and increases significantly between postnatal days 7 and 14.1 Publication

Gene expression databases

BgeeiENSMUSG00000029203.
ExpressionAtlasiP61087. baseline and differential.
GenevisibleiP61087. MM.

Interactioni

Subunit structurei

Interacts with RNF138/NARF. Interacts with BRCA1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207286. 18 interactors.
IntActiP61087. 14 interactors.
MINTiMINT-4428037.
STRINGi10090.ENSMUSP00000122471.

Structurei

3D structure databases

ProteinModelPortaliP61087.
SMRiP61087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 200UBAPROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0418. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00670000098059.
HOVERGENiHBG063308.
InParanoidiP61087.
KOiK04649.
OMAiHEMFILT.
OrthoDBiEOG091G0KVW.
PhylomeDBiP61087.
TreeFamiTF101127.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIAVQRIK REFKEVLKSE ETSKNQIKVD LVDENFTELR GEIAGPPDTP
60 70 80 90 100
YEGGRYQLEI KIPETYPFNP PKVRFITKIW HPNISSVTGA ICLDILKDQW
110 120 130 140 150
AAAMTLRTVL LSLQALLAAA EPDDPQDAVV ANQYKQNPEM FKQTARLWAH
160 170 180 190 200
VYAGAPVSSP EYTKKIENLC AMGFDRNAVI VALSSKSWDV ETATELLLSN
Length:200
Mass (Da):22,407
Last modified:January 23, 2007 - v3
Checksum:iE40668099ED25828
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163T → P in BAA24927 (PubMed:10585161).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011081 mRNA. Translation: BAA24927.1.
BC002013 mRNA. Translation: AAH02013.1.
BC085311 mRNA. Translation: AAH85311.1.
AK006316 mRNA. Translation: BAB24523.1.
CCDSiCCDS39098.1.
RefSeqiNP_058066.2. NM_016786.4.
UniGeneiMm.319512.
Mm.441014.

Genome annotation databases

EnsembliENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203.
GeneIDi53323.
KEGGimmu:53323.
UCSCiuc008xnu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011081 mRNA. Translation: BAA24927.1.
BC002013 mRNA. Translation: AAH02013.1.
BC085311 mRNA. Translation: AAH85311.1.
AK006316 mRNA. Translation: BAB24523.1.
CCDSiCCDS39098.1.
RefSeqiNP_058066.2. NM_016786.4.
UniGeneiMm.319512.
Mm.441014.

3D structure databases

ProteinModelPortaliP61087.
SMRiP61087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207286. 18 interactors.
IntActiP61087. 14 interactors.
MINTiMINT-4428037.
STRINGi10090.ENSMUSP00000122471.

PTM databases

iPTMnetiP61087.
PhosphoSitePlusiP61087.
SwissPalmiP61087.

2D gel databases

REPRODUCTION-2DPAGEP61087.

Proteomic databases

EPDiP61087.
PaxDbiP61087.
PeptideAtlasiP61087.
PRIDEiP61087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203.
GeneIDi53323.
KEGGimmu:53323.
UCSCiuc008xnu.1. mouse.

Organism-specific databases

CTDi3093.
MGIiMGI:1858216. Ube2k.

Phylogenomic databases

eggNOGiKOG0418. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00670000098059.
HOVERGENiHBG063308.
InParanoidiP61087.
KOiK04649.
OMAiHEMFILT.
OrthoDBiEOG091G0KVW.
PhylomeDBiP61087.
TreeFamiTF101127.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

ChiTaRSiUbe2k. mouse.
PROiP61087.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029203.
ExpressionAtlasiP61087. baseline and differential.
GenevisibleiP61087. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2K_MOUSE
AccessioniPrimary (citable) accession number: P61087
Secondary accession number(s): O54806
, P27924, Q16721, Q9CVV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.