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P61087

- UBE2K_MOUSE

UniProt

P61087 - UBE2K_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 K

Gene

Ube2k

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. Involved in stabilization of CASP12 during ER stress-mediated beta-amyloid neurotoxicity probably by inhibiting proteasome activity; in vitro ubiquitinates CASP12.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin protein ligase binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: UniProtKB
    5. ubiquitin-ubiquitin ligase activity Source: Ensembl

    GO - Biological processi

    1. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: Ensembl
    2. protein K48-linked ubiquitination Source: UniProtKB
    3. regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 K (EC:6.3.2.19)
    Alternative name(s):
    Huntingtin-interacting protein 2
    Short name:
    HIP-2
    Ubiquitin carrier protein
    Ubiquitin-conjugating enzyme E2-25 kDa
    Short name:
    Ubiquitin-conjugating enzyme E2(25K)
    Short name:
    Ubiquitin-conjugating enzyme E2-25K
    Ubiquitin-protein ligase
    Gene namesi
    Name:Ube2k
    Synonyms:Hip2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1858216. Ube2k.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Neurons are resistant to beta-amyloid neurotoxicity. Significantly lower CASP12 expression in brain.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 200199Ubiquitin-conjugating enzyme E2 KPRO_0000082444Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei14 – 141N6-acetyllysine; alternateBy similarity
    Cross-linki14 – 14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity

    Post-translational modificationi

    Sumoylation at Lys-14 impairs catalytic activity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP61087.
    PaxDbiP61087.
    PRIDEiP61087.

    2D gel databases

    REPRODUCTION-2DPAGEP61087.

    PTM databases

    PhosphoSiteiP61087.

    Expressioni

    Tissue specificityi

    Expressed in the brain, with highest levels found in the mitral cells of the olfactory bulb, the pyramidal cell layer of the hippocampus and the Purkinje cells of the cerebellar cortex.1 Publication

    Developmental stagei

    Expressed at all stages of brain development and increases significantly between postnatal days 7 and 14.1 Publication

    Gene expression databases

    ArrayExpressiP61087.
    BgeeiP61087.
    GenevestigatoriP61087.

    Interactioni

    Subunit structurei

    Interacts with RNF138/NARF. Interacts with BRCA1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207286. 4 interactions.
    IntActiP61087. 1 interaction.
    MINTiMINT-4428037.

    Structurei

    3D structure databases

    ProteinModelPortaliP61087.
    SMRiP61087. Positions 3-200.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini160 – 20041UBAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOVERGENiHBG063308.
    InParanoidiP61087.
    KOiK04649.
    OMAiKHWTNAY.
    OrthoDBiEOG7F513F.
    PhylomeDBiP61087.
    TreeFamiTF101127.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00627. UBA. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view]
    SMARTiSM00165. UBA. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEiPS50030. UBA. 1 hit.
    PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61087-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANIAVQRIK REFKEVLKSE ETSKNQIKVD LVDENFTELR GEIAGPPDTP    50
    YEGGRYQLEI KIPETYPFNP PKVRFITKIW HPNISSVTGA ICLDILKDQW 100
    AAAMTLRTVL LSLQALLAAA EPDDPQDAVV ANQYKQNPEM FKQTARLWAH 150
    VYAGAPVSSP EYTKKIENLC AMGFDRNAVI VALSSKSWDV ETATELLLSN 200
    Length:200
    Mass (Da):22,407
    Last modified:January 23, 2007 - v3
    Checksum:iE40668099ED25828
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti163 – 1631T → P in BAA24927. (PubMed:10585161)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011081 mRNA. Translation: BAA24927.1.
    BC002013 mRNA. Translation: AAH02013.1.
    BC085311 mRNA. Translation: AAH85311.1.
    AK006316 mRNA. Translation: BAB24523.1.
    CCDSiCCDS39098.1.
    RefSeqiNP_058066.2. NM_016786.3.
    UniGeneiMm.319512.
    Mm.441014.

    Genome annotation databases

    EnsembliENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203.
    GeneIDi53323.
    KEGGimmu:53323.
    UCSCiuc008xnu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB011081 mRNA. Translation: BAA24927.1 .
    BC002013 mRNA. Translation: AAH02013.1 .
    BC085311 mRNA. Translation: AAH85311.1 .
    AK006316 mRNA. Translation: BAB24523.1 .
    CCDSi CCDS39098.1.
    RefSeqi NP_058066.2. NM_016786.3.
    UniGenei Mm.319512.
    Mm.441014.

    3D structure databases

    ProteinModelPortali P61087.
    SMRi P61087. Positions 3-200.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207286. 4 interactions.
    IntActi P61087. 1 interaction.
    MINTi MINT-4428037.

    PTM databases

    PhosphoSitei P61087.

    2D gel databases

    REPRODUCTION-2DPAGE P61087.

    Proteomic databases

    MaxQBi P61087.
    PaxDbi P61087.
    PRIDEi P61087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000142407 ; ENSMUSP00000122471 ; ENSMUSG00000029203 .
    GeneIDi 53323.
    KEGGi mmu:53323.
    UCSCi uc008xnu.1. mouse.

    Organism-specific databases

    CTDi 3093.
    MGIi MGI:1858216. Ube2k.

    Phylogenomic databases

    eggNOGi COG5078.
    HOVERGENi HBG063308.
    InParanoidi P61087.
    KOi K04649.
    OMAi KHWTNAY.
    OrthoDBi EOG7F513F.
    PhylomeDBi P61087.
    TreeFami TF101127.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi UBE2K. mouse.
    NextBioi 310133.
    PROi P61087.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61087.
    Bgeei P61087.
    Genevestigatori P61087.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00627. UBA. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SMARTi SM00165. UBA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEi PS50030. UBA. 1 hit.
    PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Localization of huntingtin-interacting protein-2 (Hip-2) mRNA in the developing mouse brain."
      Tanno Y., Mori T., Yokoya S., Kanazawa K., Honma Y., Nikaido T., Takeda J., Tojo M., Yamamoto T., Wanaka A.
      J. Chem. Neuroanat. 17:99-107(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone and Mammary tumor.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 85-200.
      Strain: C57BL/6J.
      Tissue: Testis.
    4. Cited for: FUNCTION IN UBIQUITINATION OF CASP12, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiUBE2K_MOUSE
    AccessioniPrimary (citable) accession number: P61087
    Secondary accession number(s): O54806
    , P27924, Q16721, Q9CVV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3