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P61082 (UBC12_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NEDD8-conjugating enzyme Ubc12

EC=6.3.2.-
Alternative name(s):
NEDD8 carrier protein
NEDD8 protein ligase
Ubiquitin-conjugating enzyme E2 M
Gene names
Name:Ube2m
Synonyms:Ubc-rs2, Ubc12
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation By similarity.

Catalytic activity

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathway

Protein modification; protein neddylation.

Subunit structure

Interacts with UBA3, DCN1L and RBX1 By similarity.

Domain

Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8 By similarity.

Post-translational modification

The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183NEDD8-conjugating enzyme Ubc12
PRO_0000082489

Regions

Region1 – 2626Interaction with UBA3 By similarity
Region27 – 5731Interaction with UBA3 By similarity

Sites

Active site1111Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue31N6-acetyllysine By similarity
Modified residue501Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P61082 [UniParc].

Last modified April 26, 2004. Version 1.
Checksum: E3C288CA6A98BC5C

FASTA18320,900
        10         20         30         40         50         60 
MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF 

        70         80         90        100        110        120 
KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK 

       130        140        150        160        170        180 
PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER 


CLK 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC021792 mRNA. Translation: AAH21792.1.
RefSeqNP_663553.1. NM_145578.3.
UniGeneMm.196580.

3D structure databases

ProteinModelPortalP61082.
SMRP61082. Positions 27-183.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204404. 3 interactions.
IntActP61082. 1 interaction.
STRING10090.ENSMUSP00000005714.

PTM databases

PhosphoSiteP61082.

2D gel databases

REPRODUCTION-2DPAGEIPI00169448.
P61082.

Proteomic databases

PaxDbP61082.
PRIDEP61082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005714; ENSMUSP00000005714; ENSMUSG00000005575.
GeneID22192.
KEGGmmu:22192.
UCSCuc009ffd.2. mouse.

Organism-specific databases

CTD9040.
MGIMGI:108278. Ube2m.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00630000089859.
HOGENOMHOG000233456.
HOVERGENHBG098591.
InParanoidP61082.
KOK10579.
OrthoDBEOG7SJD64.
PhylomeDBP61082.
TreeFamTF101125.

Enzyme and pathway databases

UniPathwayUPA00885.

Gene expression databases

ArrayExpressP61082.
BgeeP61082.
GenevestigatorP61082.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302165.
PROP61082.
SOURCESearch...

Entry information

Entry nameUBC12_MOUSE
AccessionPrimary (citable) accession number: P61082
Secondary accession number(s): O76069, Q8VC50
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot