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P61082

- UBC12_MOUSE

UniProt

P61082 - UBC12_MOUSE

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Protein
NEDD8-conjugating enzyme Ubc12
Gene
Ube2m, Ubc-rs2, Ubc12
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation By similarity.

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei111 – 1111Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NEDD8 ligase activity Source: UniProtKB
  3. ribosomal S6-glutamic acid ligase activity Source: UniProtKB
  4. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein modification process Source: UniProtKB
  2. positive regulation of neuron apoptotic process Source: Ensembl
  3. protein neddylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203510. TGF-beta receptor signaling activates SMADs.
UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme Ubc12 (EC:6.3.2.-)
Alternative name(s):
NEDD8 carrier protein
NEDD8 protein ligase
Ubiquitin-conjugating enzyme E2 M
Gene namesi
Name:Ube2m
Synonyms:Ubc-rs2, Ubc12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:108278. Ube2m.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183NEDD8-conjugating enzyme Ubc12
PRO_0000082489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei3 – 31N6-acetyllysine By similarity
Modified residuei50 – 501Phosphoserine By similarity

Post-translational modificationi

The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP61082.
PaxDbiP61082.
PRIDEiP61082.

2D gel databases

REPRODUCTION-2DPAGEIPI00169448.
P61082.

PTM databases

PhosphoSiteiP61082.

Expressioni

Gene expression databases

ArrayExpressiP61082.
BgeeiP61082.
GenevestigatoriP61082.

Interactioni

Subunit structurei

Interacts with UBA3, DCN1L and RBX1 By similarity.

Protein-protein interaction databases

BioGridi204404. 4 interactions.
IntActiP61082. 1 interaction.
STRINGi10090.ENSMUSP00000005714.

Structurei

3D structure databases

ProteinModelPortaliP61082.
SMRiP61082. Positions 27-183.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5757Interaction with UBA3 By similarity
Add
BLAST

Domaini

Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8 By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00630000089859.
HOGENOMiHOG000233456.
HOVERGENiHBG098591.
InParanoidiP61082.
KOiK10579.
OMAiMDINERS.
OrthoDBiEOG7SJD64.
PhylomeDBiP61082.
TreeFamiTF101125.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61082-1 [UniParc]FASTAAdd to Basket

« Hide

MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS    50
FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY 100
HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA 150
EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK 183
Length:183
Mass (Da):20,900
Last modified:April 26, 2004 - v1
Checksum:iE3C288CA6A98BC5C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC021792 mRNA. Translation: AAH21792.1.
CCDSiCCDS20825.1.
RefSeqiNP_663553.1. NM_145578.3.
UniGeneiMm.196580.

Genome annotation databases

EnsembliENSMUST00000005714; ENSMUSP00000005714; ENSMUSG00000005575.
GeneIDi22192.
KEGGimmu:22192.
UCSCiuc009ffd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC021792 mRNA. Translation: AAH21792.1 .
CCDSi CCDS20825.1.
RefSeqi NP_663553.1. NM_145578.3.
UniGenei Mm.196580.

3D structure databases

ProteinModelPortali P61082.
SMRi P61082. Positions 27-183.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204404. 4 interactions.
IntActi P61082. 1 interaction.
STRINGi 10090.ENSMUSP00000005714.

PTM databases

PhosphoSitei P61082.

2D gel databases

REPRODUCTION-2DPAGE IPI00169448.
P61082.

Proteomic databases

MaxQBi P61082.
PaxDbi P61082.
PRIDEi P61082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005714 ; ENSMUSP00000005714 ; ENSMUSG00000005575 .
GeneIDi 22192.
KEGGi mmu:22192.
UCSCi uc009ffd.2. mouse.

Organism-specific databases

CTDi 9040.
MGIi MGI:108278. Ube2m.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00630000089859.
HOGENOMi HOG000233456.
HOVERGENi HBG098591.
InParanoidi P61082.
KOi K10579.
OMAi MDINERS.
OrthoDBi EOG7SJD64.
PhylomeDBi P61082.
TreeFami TF101125.

Enzyme and pathway databases

UniPathwayi UPA00885 .
Reactomei REACT_203510. TGF-beta receptor signaling activates SMADs.

Miscellaneous databases

NextBioi 302165.
PROi P61082.
SOURCEi Search...

Gene expression databases

ArrayExpressi P61082.
Bgeei P61082.
Genevestigatori P61082.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiUBC12_MOUSE
AccessioniPrimary (citable) accession number: P61082
Secondary accession number(s): O76069, Q8VC50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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