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Protein

NEDD8-conjugating enzyme Ubc12

Gene

Ube2m

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (By similarity).By similarity

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei111Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2173789 TGF-beta receptor signaling activates SMADs
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00885

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme Ubc12 (EC:2.3.2.-)
Alternative name(s):
NEDD8 carrier protein
Ubiquitin-conjugating enzyme E2 M
Gene namesi
Name:Ube2m
Synonyms:Ubc-rs2, Ubc12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:108278 Ube2m

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824891 – 183NEDD8-conjugating enzyme Ubc12Add BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei50PhosphoserineBy similarity1
Modified residuei169Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei169Omega-N-methylarginine; alternateCombined sources1

Post-translational modificationi

The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP61082
MaxQBiP61082
PaxDbiP61082
PeptideAtlasiP61082
PRIDEiP61082

2D gel databases

REPRODUCTION-2DPAGEiIPI00169448
P61082

PTM databases

iPTMnetiP61082
PhosphoSitePlusiP61082

Expressioni

Gene expression databases

BgeeiENSMUSG00000005575
ExpressionAtlasiP61082 baseline and differential
GenevisibleiP61082 MM

Interactioni

Subunit structurei

Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain).By similarity

Protein-protein interaction databases

BioGridi204404, 5 interactors
IntActiP61082, 1 interactor
MINTiP61082
STRINGi10090.ENSMUSP00000005714

Structurei

3D structure databases

ProteinModelPortaliP61082
SMRiP61082
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 57Interaction with UBA3By similarityAdd BLAST57

Domaini

Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.By similarity

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0420 Eukaryota
ENOG410XS81 LUCA
GeneTreeiENSGT00630000089859
HOGENOMiHOG000233456
HOVERGENiHBG098591
InParanoidiP61082
KOiK10579
OrthoDBiEOG091G0N8A
PhylomeDBiP61082
TreeFamiTF101125

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P61082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS
60 70 80 90 100
FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY
110 120 130 140 150
HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA
160 170 180
EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK
Length:183
Mass (Da):20,900
Last modified:April 26, 2004 - v1
Checksum:iE3C288CA6A98BC5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021792 mRNA Translation: AAH21792.1
CCDSiCCDS20825.1
RefSeqiNP_663553.1, NM_145578.3
UniGeneiMm.196580

Genome annotation databases

EnsembliENSMUST00000005714; ENSMUSP00000005714; ENSMUSG00000005575
GeneIDi22192
KEGGimmu:22192
UCSCiuc009ffd.2 mouse

Entry informationi

Entry nameiUBC12_MOUSE
AccessioniPrimary (citable) accession number: P61082
Secondary accession number(s): O76069, Q8VC50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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