Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P61082

- UBC12_MOUSE

UniProt

P61082 - UBC12_MOUSE

Protein

NEDD8-conjugating enzyme Ubc12

Gene

Ube2m

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (26 Apr 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation By similarity.By similarity

    Catalytic activityi

    ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei111 – 1111Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. NEDD8 ligase activity Source: UniProtKB
    3. ribosomal S6-glutamic acid ligase activity Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular protein modification process Source: UniProtKB
    2. positive regulation of neuron apoptotic process Source: Ensembl
    3. protein neddylation Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_203510. TGF-beta receptor signaling activates SMADs.
    UniPathwayiUPA00885.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NEDD8-conjugating enzyme Ubc12 (EC:6.3.2.-)
    Alternative name(s):
    NEDD8 carrier protein
    NEDD8 protein ligase
    Ubiquitin-conjugating enzyme E2 M
    Gene namesi
    Name:Ube2m
    Synonyms:Ubc-rs2, Ubc12
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:108278. Ube2m.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183NEDD8-conjugating enzyme Ubc12PRO_0000082489Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei3 – 31N6-acetyllysineBy similarity
    Modified residuei50 – 501PhosphoserineBy similarity

    Post-translational modificationi

    The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP61082.
    PaxDbiP61082.
    PRIDEiP61082.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00169448.
    P61082.

    PTM databases

    PhosphoSiteiP61082.

    Expressioni

    Gene expression databases

    ArrayExpressiP61082.
    BgeeiP61082.
    GenevestigatoriP61082.

    Interactioni

    Subunit structurei

    Interacts with UBA3, DCN1L and RBX1.By similarity

    Protein-protein interaction databases

    BioGridi204404. 4 interactions.
    IntActiP61082. 1 interaction.
    STRINGi10090.ENSMUSP00000005714.

    Structurei

    3D structure databases

    ProteinModelPortaliP61082.
    SMRiP61082. Positions 27-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 5757Interaction with UBA3By similarityAdd
    BLAST

    Domaini

    Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.By similarity

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00630000089859.
    HOGENOMiHOG000233456.
    HOVERGENiHBG098591.
    InParanoidiP61082.
    KOiK10579.
    OMAiMDINERS.
    OrthoDBiEOG7SJD64.
    PhylomeDBiP61082.
    TreeFamiTF101125.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P61082-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS    50
    FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY 100
    HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA 150
    EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK 183
    Length:183
    Mass (Da):20,900
    Last modified:April 26, 2004 - v1
    Checksum:iE3C288CA6A98BC5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC021792 mRNA. Translation: AAH21792.1.
    CCDSiCCDS20825.1.
    RefSeqiNP_663553.1. NM_145578.3.
    UniGeneiMm.196580.

    Genome annotation databases

    EnsembliENSMUST00000005714; ENSMUSP00000005714; ENSMUSG00000005575.
    GeneIDi22192.
    KEGGimmu:22192.
    UCSCiuc009ffd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC021792 mRNA. Translation: AAH21792.1 .
    CCDSi CCDS20825.1.
    RefSeqi NP_663553.1. NM_145578.3.
    UniGenei Mm.196580.

    3D structure databases

    ProteinModelPortali P61082.
    SMRi P61082. Positions 27-183.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204404. 4 interactions.
    IntActi P61082. 1 interaction.
    STRINGi 10090.ENSMUSP00000005714.

    PTM databases

    PhosphoSitei P61082.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00169448.
    P61082.

    Proteomic databases

    MaxQBi P61082.
    PaxDbi P61082.
    PRIDEi P61082.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005714 ; ENSMUSP00000005714 ; ENSMUSG00000005575 .
    GeneIDi 22192.
    KEGGi mmu:22192.
    UCSCi uc009ffd.2. mouse.

    Organism-specific databases

    CTDi 9040.
    MGIi MGI:108278. Ube2m.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00630000089859.
    HOGENOMi HOG000233456.
    HOVERGENi HBG098591.
    InParanoidi P61082.
    KOi K10579.
    OMAi MDINERS.
    OrthoDBi EOG7SJD64.
    PhylomeDBi P61082.
    TreeFami TF101125.

    Enzyme and pathway databases

    UniPathwayi UPA00885 .
    Reactomei REACT_203510. TGF-beta receptor signaling activates SMADs.

    Miscellaneous databases

    NextBioi 302165.
    PROi P61082.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61082.
    Bgeei P61082.
    Genevestigatori P61082.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiUBC12_MOUSE
    AccessioniPrimary (citable) accession number: P61082
    Secondary accession number(s): O76069, Q8VC50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3