P61079 (UB2D3_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified May 29, 2013. Version 93. History...
Names and origin
|Protein names||Recommended name:|
Ubiquitin-conjugating enzyme E2 D3
Ubiquitin carrier protein D3
Ubiquitin-conjugating enzyme E2(17)KB 3
Ubiquitin-conjugating enzyme E2-17 kDa 3
Ubiquitin-protein ligase D3
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||147 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction By similarity.
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex; when Cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened By similarity. Interacts with DAPK3. Interacts with BRCA1; the DNA damage checkpoint promotes the association with BRCA1 after ionizing radiation By similarity. Interacts non-covalently with ubiquitin By similarity. Ref.3
Belongs to the ubiquitin-conjugating enzyme family.
Sequence annotation (Features)
|AK004001 mRNA. Translation: BAB23116.1.|
AK032885 mRNA. Translation: BAC28070.1.
AK049906 mRNA. Translation: BAC33981.1.
AK077666 mRNA. Translation: BAC36940.1.
AK088448 mRNA. Translation: BAC40357.1.
BC057941 mRNA. Translation: AAH57941.1.
|RefSeq||NP_079632.1. NM_025356.4. |
3D structure databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSMUST00000106291; ENSMUSP00000101898; ENSMUSG00000078578. |
ENSMUST00000166033; ENSMUSP00000130096; ENSMUSG00000078578.
|UCSC||uc008rln.1. mouse. |
|MGI||MGI:1913355. Ube2d3. |
Enzyme and pathway databases
Gene expression databases
|GermOnline||ENSMUSG00000037096. Mus musculus. |
Family and domain databases
|Gene3D||220.127.116.11. 1 hit. |
|InterPro||IPR000608. UBQ-conjugat_E2. |
|Pfam||PF00179. UQ_con. 1 hit. |
|SUPFAM||SSF54495. UBQ-conjugat/RWD-like. 1 hit. |
|PROSITE||PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. |
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
|ChiTaRS||UBE2D3. mouse. |
|Accession||Primary (citable) accession number: P61079|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|