Reviewed, UniProtKB/Swiss-Prot P61078 (UB2D3_RAT)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 D3
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase D3
    Ubiquitin carrier protein D3
    Ubiquitin-conjugating enzyme E2-17 kDa 3
    E2(17)KB 3
    Phosphoarginine phosphatase
      Short name=PAPase

Gene names

Name:

Ube2d3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Gene Ontology (GO)
Biological process	post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Ubiquitin-conjugating enzyme E2 D3		PRO_0000082468
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61078-1 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: ADD74A8A708EFEE3	FASTA	147	16,687
	10 20 30 40 50 60 MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY 70 80 90 100 110 120 PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 130 140 PEIARIYKTD RDKYNRISRE WTQKYAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression and characterization of a ubiquitin conjugation enzyme (E2(17)kB) highly expressed in rat testis." Wing S.S., Jain P. Biochem. J. 305:125-132(1995) [PubMed: 7826319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Nomega-phosphoarginine phosphatase activity of ubiquitin-conjugating enzymes type UBC4A/10A and UBC2E." Yokoi F., Hamato N., Kamei K., Hara S., Miyagi M., Tsunasawa S., Hiraishi H., Kumon A. Res. Commun. Biochem. Cell Mol. Biol. 2:331-346(1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13177 mRNA. Translation: AAA85102.1. U13175 mRNA. Translation: AAA85100.1. AB006852 mRNA. Translation: BAA87330.1. BC072696 mRNA. Translation: AAH72696.1.
PIR	S53358.
RefSeq	NP_112516.1.
UniGene	Rn.2778
3D structure databases
HSSP	HSSP built from PDB template 1QCQ based on UniProtKB P15731.
SMR	P61078. Positions 1-147.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000013741. Rattus norvegicus. [Contig view]
GeneID	81920.
KEGG	rno:81920.
Organism-specific databases
RGD	619912. Ube2d3.
Phylogenomic databases
HOVERGEN	P61078.
Gene expression databases
ArrayExpress	P61078.
GermOnline	ENSRNOG00000013741. Rattus norvegicus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615809.

Entry information

Entry name

UB2D3_RAT

Accession

Primary (citable) accession number: P61078
Secondary accession number(s): P47986

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)