Reviewed, UniProtKB/Swiss-Prot P61073 (CXCR4_HUMAN)

Names and origin

Protein names

Recommended name:
    C-X-C chemokine receptor type 4
      Short name=CXC-R4
      Short name=CXCR-4
Alternative name(s):
    Stromal cell-derived factor 1 receptor
      Short name=SDF-1 receptor
    Fusin
    Leukocyte-derived seven transmembrane domain receptor
      Short name=LESTR
    LCR1
    FB22
    NPYRL
    HM89
    CD_antigen=CD184

Gene names

Name:

CXCR4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Ref.1 Ref.2 Ref.6 Ref.16 Ref.17
Subunit structure	Monomer. Can form dimers. Interacts with CD164. Interacts with HIV-1 surface protein gp120 and Tat. Interacts with ARRB2. Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.28 Ref.31
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
Domain	The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity. Ref.22
Post-translational modification	Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. Ref.24 Ref.26
Involvement in disease	Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis. Ref.27
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally (Ref.1 and Ref.2) thought to be a receptor for neuropeptide Y type 3 (NPY3R) (NPY3-R).

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein Proteoglycan Sulfation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.23 Inferred from direct assay. Source: UniProtKB activation of MAPK activity Traceable author statement. Source: ProtInc apoptosis Traceable author statement. Source: ProtInc chemotaxis Traceable author statement. Source: ProtInc elevation of cytosolic calcium ion concentration Traceable author statement. Source: ProtInc immune response Traceable author statement. Source: ProtInc inflammatory response Ref.4 Traceable author statement. Source: ProtInc initiation of viral infection Inferred from Experiment. Source: Reactome interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW response to hypoxia Inferred from expression pattern. Source: UniProtKB response to virus Traceable author statement. Source: ProtInc
Cellular component	cell leading edge Inferred from direct assay. Source: UniProtKB cytoplasm Traceable author statement. Source: ProtInc integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: ProtInc
Molecular function	C-C chemokine receptor activity Inferred from electronic annotation. Source: InterPro C-X-C chemokine receptor activity Ref.7 Non-traceable author statement. Source: UniProtKB actin binding Inferred from direct assay. Source: UniProtKB coreceptor activity Traceable author statement. Source: ProtInc myosin light chain binding Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYH9	P35579	4	EBI-489411,EBI-350338

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P61073-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P61073-2)

Also known as: CXCR4-LO;

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MEGIS → MSIPLPLLQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	C-X-C chemokine receptor type 4		PRO_0000069352
Regions
	Topological domain	1 – 39	39	Extracellular Potential
	Transmembrane	40 – 63	24	1 Potential
	Topological domain	64 – 79	16	Cytoplasmic Potential
	Transmembrane	80 – 99	20	2 Potential
	Topological domain	100 – 110	11	Extracellular Potential
	Transmembrane	111 – 132	22	3 Potential
	Topological domain	133 – 154	22	Cytoplasmic Potential
	Transmembrane	155 – 175	21	4 Potential
	Topological domain	176 – 200	25	Extracellular Potential
	Transmembrane	201 – 220	20	5 Potential
	Topological domain	221 – 240	20	Cytoplasmic Potential
	Transmembrane	241 – 261	21	6 Potential
	Topological domain	262 – 285	24	Extracellular Potential
	Transmembrane	286 – 305	20	7 Potential
	Topological domain	306 – 352	47	Cytoplasmic Potential
	Motif	133 – 135	3	Important for signaling
Amino acid modifications
	Modified residue	21	1	Sulfotyrosine Ref.26 Ref.31
	Modified residue	319	1	Phosphoserine Ref.29 Ref.30
	Modified residue	348	1	Phosphoserine Ref.30
	Modified residue	351	1	Phosphoserine Ref.30
	Glycosylation	11	1	N-linked (GlcNAc...) Ref.24
	Glycosylation	18	1	O-linked (Xyl...) (chondroitin sulfate) Ref.26
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 186		By similarity
Natural variations
	Alternative sequence	1 – 5	5	MEGIS → MSIPLPLLQ in isoform 2.		VSP_001890
Experimental info
	Mutagenesis	7	1	Y → F: Sulfate incorporation greatly reduced; when associated with F-12 and F-21. Moderate reduction in sulfate incorporation; when associated with F-12 and A-18. No sulfate incorporation and binding PDF1alpha greatly reduced; when associated with F-12; A-18 and F-21. Ref.26
	Mutagenesis	8	1	T → A: No effect on sulfate incorporation; when associated with A-9 and A-13. Ref.26
	Mutagenesis	9	1	S → A: No effect on sulfate incorporation; when associated with A-8 and A-13. Ref.26
	Mutagenesis	11	1	N → A: Reduced molecular weight. Enhanced coreceptor activity on R5 HIV-1 isolate Envs. Slight further enhancement of coreceptor activity; when associated with A-13. Ref.24
	Mutagenesis	12	1	Y → F: Sulfate incorporation greatly reduced; when associated with F-7 and F-21. Moderate reduction in sulfate incorporation; when associated with F-7 and A-18. No sulfate incorporation and binding PDF1alpha greatly reduced; when associated with F-7; A-18 and F-21. Ref.26
	Mutagenesis	13	1	T → A: Enhanced coreceptor activity on R5 HIV-1 isolate Envs. No effect on sulfate incorporation; when associated with A-8 and A-9. Ref.24
	Mutagenesis	18	1	S → A: Sulfate incorporation greatly reduced; when associated with F-21. Moderate reduction in sulfate incorporation; when associated with F-7 and F-12. No sulfate incorporation and binding PDF1alpha greatly reduced; when associated with F-7; F-12; and F-21. Ref.26
	Mutagenesis	21	1	Y → F: Sulfate incorporation greatly reduced; when associated with F-7 and F-12. Sulfate incorporation greatly reduced; when associated with A-18. No sulfate incorporation and binding PDF1alpha greatly reduced; when associated with F-7; F-12 and A-18. Ref.26
	Mutagenesis	176	1	N → A: Enhanced coreceptor activity on R5 HIV-1 isolate Envs; when associated with A-11. Ref.24

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: 8C8476A186786B83	FASTA	352	39,746
	10 20 30 40 50 60 MEGISIYTSD NYTEEMGSGD YDSMKEPCFR EENANFNKIF LPTIYSIIFL TGIVGNGLVI 70 80 90 100 110 120 LVMGYQKKLR SMTDKYRLHL SVADLLFVIT LPFWAVDAVA NWYFGNFLCK AVHVIYTVNL 130 140 150 160 170 180 YSSVLILAFI SLDRYLAIVH ATNSQRPRKL LAEKVVYVGV WIPALLLTIP DFIFANVSEA 190 200 210 220 230 240 DDRYICDRFY PNDLWVVVFQ FQHIMVGLIL PGIVILSCYC IIISKLSHSK GHQKRKALKT 250 260 270 280 290 300 TVILILAFFA CWLPYYIGIS IDSFILLEII KQGCEFENTV HKWISITEAL AFFHCCLNPI 310 320 330 340 350 LYAFLGAKFK TSAQHALTSV SRGSSLKILS KGKRGGHSSV STESESSSFH SS
	Isoform 2 (CXCR4-LO) [UniParc] [UniParc]. Checksum: 83F9E099F41FAB9B	FASTA	356	40,221
	10 20 30 40 50 60 MSIPLPLLQI YTSDNYTEEM GSGDYDSMKE PCFREENANF NKIFLPTIYS IIFLTGIVGN 70 80 90 100 110 120 GLVILVMGYQ KKLRSMTDKY RLHLSVADLL FVITLPFWAV DAVANWYFGN FLCKAVHVIY 130 140 150 160 170 180 TVNLYSSVLI LAFISLDRYL AIVHATNSQR PRKLLAEKVV YVGVWIPALL LTIPDFIFAN 190 200 210 220 230 240 VSEADDRYIC DRFYPNDLWV VVFQFQHIMV GLILPGIVIL SCYCIIISKL SHSKGHQKRK 250 260 270 280 290 300 ALKTTVILIL AFFACWLPYY IGISIDSFIL LEIIKQGCEF ENTVHKWISI TEALAFFHCC 310 320 330 340 350 LNPILYAFLG AKFKTSAQHA LTSVSRGSSL KILSKGKRGG HSSVSTESES SSFHSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, characterization, and localization of the human homolog to the reported bovine NPY Y3 receptor: lack of NPY binding and activation." Herzog H., Hort Y.J., Shine J., Selbie L.A. DNA Cell Biol. 12:465-471(1993) [PubMed: 8329116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PRELIMINARY FUNCTION. Tissue: Lung.
[2]	"A proposed bovine neuropeptide Y (NPY) receptor cDNA clone, or its human homologue, confers neither NPY binding sites nor NPY responsiveness on transfected cells." Jazin E.E., Yoo H., Blomqvist A.G., Yee F., Weng G., Walker M.W., Salon J., Larhammar D., Wahlestedt C.R. Regul. Pept. 47:247-258(1993) [PubMed: 8234909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PRELIMINARY FUNCTION. Tissue: Fetal brain.
[3]	"Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated from human spleen." Federsppiel B., Melhado I.G., Duncan A.M.V., Delaney A.D., Schappert K., Clark-Lewis I., Jirik F.R. Genomics 16:707-712(1993) [PubMed: 8325644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal spleen.
[4]	"Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes." Loetscher M., Geiser T., O'Reilly T., Zwahlen R., Baggiolini M., Moser B. J. Biol. Chem. 269:232-237(1994) [PubMed: 8276799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Monocyte.
[5]	"Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors." Nomura H., Nielsen B.W., Matsushima K. Int. Immunol. 5:1239-1249(1993) [PubMed: 7505609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[6]	"HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." Feng Y., Broder C.C., Kennedy P.E., Berger E.A. Science 272:872-877(1996) [PubMed: 8629022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION OF ITS HIV-1 CORECEPTOR FUNCTION.
[7]	"Genomic organization and functional characterization of the chemokine receptor CXCR4, a major entry co-receptor for human immunodeficiency virus type 1." Wegner S.A., Ehrenberg P.K., Chang G., Dayhoff D.E., Sleeker A.L., Michael N.L. J. Biol. Chem. 273:4754-4760(1998) [PubMed: 9468539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Peripheral blood leukocyte.
[8]	"Genomic organization and promoter characterization of human CXCR4 gene." Caruz A., Samsom M., Alonso J.M., Alcami J., Baleux F., Virelizier J.-L., Parmentier M., Arenzana-Seisdedos F. FEBS Lett. 426:271-278(1998) [PubMed: 9599023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[9]	"Partial resistance to infection by R5X4 primary HIV type 1 isolates in an exposed-uninfected individual homozygous for CCR5 32-base pair deletion." Xiao L., Weiss S.H., Qari S.H., Rudolph D., Zhao C., Denny T.N., Hodge T., Lal R.B. AIDS Res. Hum. Retroviruses 15:1201-1208(1999) [PubMed: 10480633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[10]	"Genomic organization and expression of the CXCR4 gene in mouse and man: absence of a splice variant corresponding to mouse CXCR4-B in human tissues." Frodl R., Gierschik P., Moepps B. J. Recept. Signal Transduct. 18:321-344(1998) [PubMed: 9879064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Peripheral blood lymphocyte.
[11]	"CXCR4-Lo: molecular cloning and functional expression of a novel human CXCR4 splice variant." Gupta S.K., Pillarisetti K. J. Immunol. 163:2368-2372(1999) [PubMed: 10452968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Neutrophil.
[12]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Warren C.N., Aronstam R.S., Sharma S.V. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[13]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[14]	SeattleSNPs variation discovery resource Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[15]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon.
[16]	"The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry." Bleul C.C., Farzan M., Choe H., Parolin C., Clark-Lewis I., Sodroski J., Springer T.A. Nature 382:829-833(1996) [PubMed: 8752280] [Abstract] Cited for: FUNCTION.
[17]	"The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1." Oberlin E., Amara A., Bachelerie F., Bessia C., Virelizier J.-L., Arenzana-Seisdedos F., Schwartz O., Heard J.-M., Clark-Lewis I., Legler D.F., Loetscher M., Baggiolini M., Moser B. Nature 382:833-835(1996) [PubMed: 8752281] [Abstract] Cited for: FUNCTION.
[18]	Erratum Oberlin E., Amara A., Bachelerie F., Bessia C., Virelizier J.-L., Arenzana-Seisdedos F., Schwartz O., Heard J.-M., Clark-Lewis I., Legler D.F., Loetscher M., Baggiolini M., Moser B. Nature 384:288-288(1996)
[19]	"Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines." Lapham C.K., Ouyang J., Chandrasekhar B., Nguyen N.Y., Dimitrov D.S., Golding H. Science 274:602-605(1996) [PubMed: 8849450] [Abstract] Cited for: CHARACTERIZATION AS HIV-1 CORECEPTOR.
[20]	"CD4-independent infection by HIV-2 is mediated by fusin/CXCR4." Endres M.J., Clapham P.R., Marsh M., Ahuja M., Turner J.D., McKnight A., Thomas J.F., Stoebenau-Haggarty B., Choe S., Vance P.J., Wells T.N.C., Power C.A., Sutterwala S.S., Doms R.W., Landau N.R., Hoxie J.A. Cell 87:745-756(1996) [PubMed: 8929542] [Abstract] Cited for: CHARACTERIZATION AS HIV-2 PRIMARY RECEPTOR IN SOME ISOLATES.
[21]	"Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry." Farzan M., Mirzabekov T., Kolchinsky P., Wyatt R., Cayabyab M., Gerard N.P., Gerard C., Sodroski J., Choe H. Cell 96:667-676(1999) [PubMed: 10089882] [Abstract] Cited for: SULFATION.
[22]	"Identification of CXCR4 domains that support coreceptor and chemokine receptor functions." Doranz B.J., Orsini M.J., Turner J.D., Hoffman T.L., Berson J.F., Hoxie J.A., Peiper S.C., Brass L.F., Doms R.W. J. Virol. 73:2752-2761(1999) [PubMed: 10074122] [Abstract] Cited for: DOMAINS, INTERACTION WITH HIV-1 SURFACE PROTEIN GP120.
[23]	"beta-arrestin differentially regulates the chemokine receptor CXCR4-mediated signaling and receptor internalization, and this implicates multiple interaction sites between beta-arrestin and CXCR4." Cheng Z.J., Zhao J., Sun Y., Hu W., Wu Y.L., Cen B., Wu G.-X., Pei G. J. Biol. Chem. 275:2479-2485(2000) [PubMed: 10644702] [Abstract] Cited for: INTERACTION WITH ARRB2.
[24]	"N-linked glycosylation of CXCR4 masks coreceptor function for CCR5-dependent human immunodeficiency virus type 1 isolates." Chabot D.J., Chen H., Dimitrov D.S., Broder C.C. J. Virol. 74:4404-4413(2000) [PubMed: 10756055] [Abstract] Cited for: GLYCOSYLATION AT ASN-11, INTERACTION WITH HIV-1 ENV, SUBUNIT, MUTAGENESIS OF ASN-11; THR-13 AND ASN-176.
[25]	"Selective CXCR4 antagonism by Tat: implications for in vivo expansion of coreceptor use by HIV-1." Xiao H., Neuveut C., Tiffany H.L., Benkirane M., Rich E.A., Murphy P.M., Jeang K.-T. Proc. Natl. Acad. Sci. U.S.A. 97:11466-11471(2000) [PubMed: 11027346] [Abstract] Cited for: INTERACTION WITH HIV-1 TAT.
[26]	"The role of post-translational modifications of the CXCR4 amino terminus in stromal-derived factor 1 alpha association and HIV-1 entry." Farzan M., Babcock G.J., Vasilieva N., Wright P.L., Kiprilov E., Mirzabekov T., Choe H. J. Biol. Chem. 277:29484-29489(2002) [PubMed: 12034737] [Abstract] Cited for: GLYCOSYLATION AT SER-18, IDENTIFICATION OF PROTEOGLYCAN, INTERACTION WITH CXCL12, SULFATION AT TYR-21, MUTAGENESIS OF TYR-7; THR-8; SER-9; TYR-12; SER-18 AND TYR-21.
[27]	"Mutations in the chemokine receptor gene CXCR4 are associated with WHIM syndrome, a combined immunodeficiency disease." Hernandez P.A., Gorlin R.J., Lukens J.N., Taniuchi S., Bohinjec J., Francois F., Klotman M.E., Diaz G.A. Nat. Genet. 34:70-74(2003) [PubMed: 12692554] [Abstract] Cited for: DISEASE.
[28]	"Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133+ cells." Forde S., Tye B.J., Newey S.E., Roubelakis M., Smythe J., McGuckin C.P., Pettengell R., Watt S.M. Blood 109:1825-1833(2007) [PubMed: 17077324] [Abstract] Cited for: INTERACTION WITH CD164.
[29]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, MASS SPECTROMETRY.
[30]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319; SER-348 AND SER-351, MASS SPECTROMETRY.
[31]	"Recognition of a CXCR4 sulfotyrosine by the chemokine stromal cell-derived factor-1alpha (SDF-1alpha/CXCL12)." Veldkamp C.T., Seibert C., Peterson F.C., Sakmar T.P., Volkman B.F. J. Mol. Biol. 359:1400-1409(2006) [PubMed: 16725153] [Abstract] Cited for: STRUCTURE BY NMR OF 1-38, SULFATION AT TYR-21, MASS SPECTROMETRY, INTERACTION WITH CXCL12.
+	Additional computationally mapped references.

Web resources

CXCR4base

CXCR4 mutation db

Wikipedia

CXC chemokine receptors entry

Wikipedia

CXCR4 entry

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L01639 mRNA. Translation: AAA16594.1.
M99293 mRNA. Translation: AAA16617.1.
X71635 mRNA. Translation: CAA50641.1.
L06797 mRNA. Translation: AAA03209.1.
D10924 mRNA. Translation: BAA01722.1.
AF005058 Genomic DNA. Translation: AAB93982.1.
AF052572 Genomic DNA. Translation: AAC34581.1.
AF025375 mRNA. Translation: AAB81970.1.
Y14739 Genomic DNA. Translation: CAA75034.1.
AJ224869 Genomic DNA. Translation: CAA12166.1. Sequence problems.
AF147204 mRNA. Translation: AAF00130.1.
AY242129 mRNA. Translation: AAO92296.1.
BT006660 mRNA. Translation: AAP35306.1.
AY728138 Genomic DNA. Translation: AAU05775.1.
BC020968 mRNA. Translation: AAH20968.1.

IPI

IPI00028159.
IPI00216445.

PIR

A45747.

RefSeq

NP_001008540.1.
NP_003458.1.

UniGene

Hs.593413

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K03	NMR	-	B/D	1-38	[»]
2K04	NMR	-	B/D	1-38	[»]
2K05	NMR	-	B/D	1-38	[»]

SMR

P61073. Positions 37-318.

ModBase

Search...

Protein-protein interaction databases

IntAct

P61073. 2 interactions.

STRING

P61073.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P61073.

Proteomic databases

PRIDE

P61073.

Genome annotation databases

Ensembl

ENST00000241393; ENSP00000241393; ENSG00000121966; Homo sapiens. [Genome view]

GeneID

7852.

KEGG

hsa:7852.

UCSC

uc002tuy.1. human.
uc002tuz.1. human.

Organism-specific databases

CTD

7852.

GeneCards

GC02M136588.

H-InvDB

HIX0002482.

HGNC

HGNC:2561. CXCR4.

HPA

CAB011447.

MIM

162643. gene.
193670. phenotype.

Orphanet

51636. WHIM syndrome.

PharmGKB

PA27058.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18826.

HOVERGEN

P61073.

OMA

FNRIFLP.

Enzyme and pathway databases

Pathway_Interaction_DB

ephrinbrevpathway. Ephrin B reverse signaling.
hif1_tfpathway. HIF-1-alpha transcription factor network.
s1p_s1p3_pathway. S1P3 pathway.
syndecan_4_pathway. Syndecan-4-mediated signaling events.

Reactome

REACT_14797. Signaling by GPCR.
REACT_6185. HIV Infection.

Gene expression databases

ArrayExpress

P61073.

Bgee

P61073.

CleanEx

HS_CXCR4.

Genevestigator

P61073.

GermOnline

ENSG00000121966. Homo sapiens.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR000355. Chmkine_rcpt.
IPR001277. CXC_4_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00657. CCCHEMOKINER.
PR00645. CXCCHMKINER4.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00452. Framycetin.

NextBio

30286.

PMAP-CutDB

P61073.

SOURCE

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Entry information

Entry name

CXCR4_HUMAN

Accession

Primary (citable) accession number: P61073
Secondary accession number(s): O60835 , P30991, P56438, Q9UKN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.