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Protein

Ras-related protein Rab-10

Gene

RAB10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.By similarity3 Publications

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP) (By similarity). That Rab is activated by the DENND4C guanine exchange factor (GEF).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 23GTPBy similarity8
Nucleotide bindingi64 – 68GTPBy similarity5
Nucleotide bindingi122 – 125GTPBy similarity4

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • GDP binding Source: UniProtKB
  • GTPase activity Source: Reactome
  • GTP binding Source: UniProtKB
  • myosin V binding Source: UniProtKB

GO - Biological processi

  • antigen processing and presentation Source: UniProtKB
  • axonogenesis Source: UniProtKB
  • basolateral protein localization Source: UniProtKB
  • cellular response to insulin stimulus Source: UniProtKB
  • endoplasmic reticulum tubular network organization Source: UniProtKB
  • endosomal transport Source: UniProtKB
  • establishment of neuroblast polarity Source: UniProtKB
  • establishment of protein localization to endoplasmic reticulum membrane Source: UniProtKB
  • establishment of protein localization to membrane Source: UniProtKB
  • Golgi to plasma membrane protein transport Source: UniProtKB
  • Golgi to plasma membrane transport Source: UniProtKB
  • membrane organization Source: Reactome
  • polarized epithelial cell differentiation Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000084733-MONOMER.
ReactomeiR-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-10
Gene namesi
Name:RAB10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9759. RAB10.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cell-cell junction Source: BHF-UCL
  • cilium Source: UniProtKB
  • cytoplasmic vesicle membrane Source: Reactome
  • endoplasmic reticulum membrane Source: UniProtKB
  • endoplasmic reticulum tubular network Source: UniProtKB
  • endosome Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • insulin-responsive compartment Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • recycling endosome Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23T → N: Probable dominant negative mutant locked in the inactive GDP-bound form; alters the basolateral recycling pathway in epithelial cells and endoplasmic reticulum membrane morphology. 2 Publications1
Mutagenesisi68Q → L: Probable constitutively active mutant unable to hydrolyze GTP; accumulates at the base of the primary cilium and alters the basolateral recycling pathway in epithelial cells. 1 Publication1

Organism-specific databases

DisGeNETi10890.
OpenTargetsiENSG00000084733.
PharmGKBiPA34100.

Polymorphism and mutation databases

BioMutaiRAB10.
DMDMi46577638.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211461 – 200Ras-related protein Rab-10Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102N6-acetyllysineCombined sources1
Lipidationi199S-geranylgeranyl cysteineBy similarity1
Lipidationi200S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

EPDiP61026.
MaxQBiP61026.
PaxDbiP61026.
PeptideAtlasiP61026.
PRIDEiP61026.
TopDownProteomicsiP61026.

PTM databases

iPTMnetiP61026.
PhosphoSitePlusiP61026.
SwissPalmiP61026.

Expressioni

Gene expression databases

BgeeiENSG00000084733.
CleanExiHS_RAB10.
GenevisibleiP61026. HS.

Organism-specific databases

HPAiHPA045611.

Interactioni

Subunit structurei

Interacts with MYO5A; mediates the transport to the plasma membrane of SLC2A4/GLUT4 storage vesicles. Interacts with GDI1 and maybe with GDI2; negatively regulates RAB10 association with membranes and activation. Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1 (By similarity). Interacts with EXOC4; probably associates with the exocyst (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0075EBI-726075,EBI-5323863
OPTNQ96CV93EBI-726075,EBI-748974

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • myosin V binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116096. 50 interactors.
IntActiP61026. 28 interactors.
MINTiMINT-1404404.
STRINGi9606.ENSP00000264710.

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi22 – 31Combined sources10
Beta strandi43 – 53Combined sources11
Beta strandi56 – 65Combined sources10
Helixi69 – 71Combined sources3
Helixi72 – 75Combined sources4
Helixi76 – 78Combined sources3
Turni79 – 81Combined sources3
Beta strandi83 – 90Combined sources8
Helixi94 – 98Combined sources5
Helixi100 – 110Combined sources11
Beta strandi116 – 122Combined sources7
Helixi127 – 129Combined sources3
Helixi134 – 143Combined sources10
Beta strandi147 – 150Combined sources4
Turni153 – 156Combined sources4
Beta strandi157 – 159Combined sources3
Helixi160 – 172Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LPNX-ray2.80A/C1-175[»]
5SZJX-ray2.66A1-200[»]
ProteinModelPortaliP61026.
SMRiP61026.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 46Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00860000133675.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP61026.
KOiK07903.
OMAiREHSIRF.
OrthoDBiEOG091G0LA6.
PhylomeDBiP61026.
TreeFamiTF314097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT
60 70 80 90 100
VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI
110 120 130 140 150
SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE
160 170 180 190 200
TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
Length:200
Mass (Da):22,541
Last modified:April 26, 2004 - v1
Checksum:i7F02B8E8E46EE1E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138E → G in CAB66585 (PubMed:11230166).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086917 mRNA. Translation: AAP97147.1.
AF297660 mRNA. Translation: AAG13413.1.
AF106681 mRNA. Translation: AAD43034.1.
AL136650 mRNA. Translation: CAB66585.1.
AK023223 mRNA. Translation: BAB14474.1.
AF498945 mRNA. Translation: AAM21093.1.
CR457303 mRNA. Translation: CAG33584.1.
CH471053 Genomic DNA. Translation: EAX00710.1.
CH471053 Genomic DNA. Translation: EAX00711.1.
BC000896 mRNA. Translation: AAH00896.1.
CCDSiCCDS1720.1.
RefSeqiNP_057215.3. NM_016131.4.
UniGeneiHs.467960.

Genome annotation databases

EnsembliENST00000264710; ENSP00000264710; ENSG00000084733.
GeneIDi10890.
KEGGihsa:10890.
UCSCiuc002rgv.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086917 mRNA. Translation: AAP97147.1.
AF297660 mRNA. Translation: AAG13413.1.
AF106681 mRNA. Translation: AAD43034.1.
AL136650 mRNA. Translation: CAB66585.1.
AK023223 mRNA. Translation: BAB14474.1.
AF498945 mRNA. Translation: AAM21093.1.
CR457303 mRNA. Translation: CAG33584.1.
CH471053 Genomic DNA. Translation: EAX00710.1.
CH471053 Genomic DNA. Translation: EAX00711.1.
BC000896 mRNA. Translation: AAH00896.1.
CCDSiCCDS1720.1.
RefSeqiNP_057215.3. NM_016131.4.
UniGeneiHs.467960.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LPNX-ray2.80A/C1-175[»]
5SZJX-ray2.66A1-200[»]
ProteinModelPortaliP61026.
SMRiP61026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116096. 50 interactors.
IntActiP61026. 28 interactors.
MINTiMINT-1404404.
STRINGi9606.ENSP00000264710.

PTM databases

iPTMnetiP61026.
PhosphoSitePlusiP61026.
SwissPalmiP61026.

Polymorphism and mutation databases

BioMutaiRAB10.
DMDMi46577638.

Proteomic databases

EPDiP61026.
MaxQBiP61026.
PaxDbiP61026.
PeptideAtlasiP61026.
PRIDEiP61026.
TopDownProteomicsiP61026.

Protocols and materials databases

DNASUi10890.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264710; ENSP00000264710; ENSG00000084733.
GeneIDi10890.
KEGGihsa:10890.
UCSCiuc002rgv.4. human.

Organism-specific databases

CTDi10890.
DisGeNETi10890.
GeneCardsiRAB10.
HGNCiHGNC:9759. RAB10.
HPAiHPA045611.
MIMi612672. gene.
neXtProtiNX_P61026.
OpenTargetsiENSG00000084733.
PharmGKBiPA34100.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00860000133675.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP61026.
KOiK07903.
OMAiREHSIRF.
OrthoDBiEOG091G0LA6.
PhylomeDBiP61026.
TreeFamiTF314097.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000084733-MONOMER.
ReactomeiR-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiRAB10. human.
GeneWikiiRAB10.
GenomeRNAii10890.
PROiP61026.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000084733.
CleanExiHS_RAB10.
GenevisibleiP61026. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB10_HUMAN
AccessioniPrimary (citable) accession number: P61026
Secondary accession number(s): D6W538
, O88386, Q6IA52, Q9D7X6, Q9H0T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.