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Reviewed, UniProtKB/Swiss-Prot P61023 (CHP1_RAT)

Last modified January 19, 2010. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Calcium-binding protein p22
Alternative name(s):
    Calcium-binding protein CHP
    Calcineurin homologous protein
Gene names
Name: Chp
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Required for constitutive membrane traffic. Inhibits GTPase-stimulated Na+/H+ exchange. Also inhibits calcineurin phosphatase activity. Required for activity of SLC9A1/NHE1.

Subunit structure

Specifically binds to SLC9A1/NHE1 at a domain that is critical for growth factor stimulation of exchange activity By similarity. Monomer. Ref.4

Subcellular location

Cytoplasm.

Tissue specificity

Ubiquitously expressed.

Post-translational modification

Both N-myristoylation and calcium-mediated conformational changes are essential for its function.

Sequence similarities

Contains 4 EF-hand domains.

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Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRepeat
   LigandCalcium
Metal-binding
   PTMLipoprotein
Myristate
   Technical term3D-structure
Gene Ontology (GO)
   Biological processcalcium ion-dependent exocytosis Ref.1

Inferred from direct assay. Source: RGD

calcium-mediated signaling Ref.1

Traceable author statement. Source: RGD

transcytosis Ref.1

Inferred from genetic interaction. Source: RGD

   Cellular componentcytosol Ref.1

Inferred from direct assay. Source: RGD

   Molecular functioncalcium ion binding Ref.1

Inferred from direct assay. Source: RGD

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Probable
Chain2 – 195194Calcium-binding protein p22
PRO_0000073846

Regions

Domain26 – 6136EF-hand 1
Domain71 – 10636EF-hand 2
Domain110 – 14536EF-hand 3
Domain151 – 18636EF-hand 4
Calcium binding123 – 134121
Calcium binding164 – 175122

Amino acid modifications

Lipidation21N-myristoyl glycine Probable

Experimental info

Mutagenesis1341E → A: Loss of targeting/fusion function.

Secondary structure

1 ................................ 195
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P61023-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 7B803EF0ABED829E

FASTA19522,432
        10         20         30         40         50         60 
MGSRASTLLR DEELEEIKKE TGFSHSQITR LYSRFTSLDK GENGTLSRED FQRIPELAIN 

        70         80         90        100        110        120 
PLGDRIINAF FSEGEDQVNF RGFMRTLAHF RPIEDNEKSK DVNGPEPLNS RSNKLHFAFR 

       130        140        150        160        170        180 
LYDLDKDDKI SRDELLQVLR MMVGVNISDE QLGSIADRTI QEADQDGDSA ISFTEFVKVL 

       190 
EKVDVEQKMS IRFLH

« Hide

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References

« Hide 'large scale' references
[1]"A novel Ca2+-binding protein, p22, is required for constitutive membrane traffic."
Barroso M.R., Bernd K.K., Dewitt N.D., Chang A., Mills K., Sztul E.S.
J. Biol. Chem. 271:10183-10187(1996) [PubMed: 8626580] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"A serine/threonine kinase which causes apoptosis-like cell death interacts with a calcineurin B-like protein capable of binding Na+/H+ exchanger."
Matsumoto M., Miyake Y., Nagita M., Inoue H., Shitakubo D., Takemoto K., Ohtsuka C., Murakami H., Nakamura N., Kanazawa H.
J. Biochem. 130:217-225(2001) [PubMed: 11481038] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[4]"Structural characterization of calcineurin B homologous protein 1."
Naoe Y., Arita K., Hashimoto H., Kanazawa H., Sato M., Shimizu T.
J. Biol. Chem. 280:32372-32378(2005) [PubMed: 15987692] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH CALCIUM IONS, SUBUNIT.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U39875 mRNA. Translation: AAB04146.1.
AB070350 mRNA. Translation: BAB63369.1.
BC062029 mRNA. Translation: AAH62029.1.
IPIIPI00394419.
RefSeqNP_077053.1.
UniGeneRn.11041

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CT9X-ray2.20A/B1-195[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP61023.

PTM databases

PhosphoSiteP61023.

Proteomic databases

PRIDEP61023.

Genome annotation databases

EnsemblENSRNOT00000056405; ENSRNOP00000053243; ENSRNOG00000004742; Rattus norvegicus. [Genome view]
GeneID64152.
KEGGrno:64152.
UCSCNM_024139. rat.

Organism-specific databases

CTD64152.
RGD620447. Chp.

Phylogenomic databases

eggNOGroNOG10716.
HOVERGENP61023.
OMAREDFLRI.
PhylomeDBP61023.

Gene expression databases

GenevestigatorP61023.
GermOnlineENSRNOG00000004742. Rattus norvegicus.
ENSRNOG00000008946. Rattus norvegicus.

Family and domain databases

InterProIPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio612777.

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Entry information

Entry nameCHP1_RAT
AccessionPrimary (citable) accession number: P61023
Secondary accession number(s): Q62877
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: January 19, 2010
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents