P61018 (RAB4B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-4B

Gene names

Name:	RAB4B
ORF Names:	PP1596

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein transport. Probably involved in vesicular traffic By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Non-traceable author statement. Source: UniProtKB
Cellular_component	intracellular Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P61018-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P61018-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MAETY → MSVSLPLTVMVRERDWIGIHLFSLYLSLPVGIPDFGSIWS

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 213

213

Ras-related protein Rab-4B

PRO_0000121099

Regions

Nucleotide binding

15 – 23

9

GTP

Nucleotide binding

63 – 67

5

GTP By similarity

Nucleotide binding

121 – 124

4

GTP

Nucleotide binding

151 – 153

3

GTP

Motif

37 – 45

9

Effector region By similarity

Amino acid modifications

Modified residue

213

1

Cysteine methyl ester By similarity

Lipidation

211

1

S-geranylgeranyl cysteine By similarity

Lipidation

213

1

S-geranylgeranyl cysteine By similarity

Natural variations

Alternative sequence

1 – 5

5

MAETY → MSVSLPLTVMVRERDWIGIH LFSLYLSLPVGIPDFGSIWS in isoform 2.

VSP_013567

Experimental info

Sequence conflict

4 – 6

3

TYD → DRH in AAP97171. Ref.1

Secondary structure

1

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213

Helix Strand Turn

Details...

Beta strand

7 – 16

10

Helix

21 – 29

9

Beta strand

45 – 52

8

Beta strand

55 – 62

8

Helix

67 – 70

4

Helix

75 – 78

4

Beta strand

82 – 89

8

Helix

93 – 97

5

Helix

99 – 109

11

Beta strand

115 – 121

7

Helix

123 – 128

6

Helix

133 – 142

10

Beta strand

146 – 150

5

Turn

152 – 154

3

Helix

158 – 174

17

Helix

180 – 182

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: 0C3D76DC3285DB98	FASTA	213	23,587
	10 20 30 40 50 60 MAETYDFLFK FLVIGSAGTG KSCLLHQFIE NKFKQDSNHT IGVEFGSRVV NVGGKTVKLQ 70 80 90 100 110 120 IWDTAGQERF RSVTRSYYRG AAGALLVYDI TSRETYNSLA AWLTDARTLA SPNIVVILCG 130 140 150 160 170 180 NKKDLDPERE VTFLEASRFA QENELMFLET SALTGENVEE AFLKCARTIL NKIDSGELDP 190 200 210 ERMGSGIQYG DASLRQLRQP RSAQAVAPQP CGC
	Isoform 2 [UniParc]. Checksum: 69EBDC77A684EAC6	FASTA	248	27,506
	10 20 30 40 50 60 MSVSLPLTVM VRERDWIGIH LFSLYLSLPV GIPDFGSIWS DFLFKFLVIG SAGTGKSCLL 70 80 90 100 110 120 HQFIENKFKQ DSNHTIGVEF GSRVVNVGGK TVKLQIWDTA GQERFRSVTR SYYRGAAGAL 130 140 150 160 170 180 LVYDITSRET YNSLAAWLTD ARTLASPNIV VILCGNKKDL DPEREVTFLE ASRFAQENEL 190 200 210 220 230 240 MFLETSALTG ENVEEAFLKC ARTILNKIDS GELDPERMGS GIQYGDASLR QLRQPRSAQA VAPQPCGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel human cDNA homologous to R.norvegicus rab4b mRNA." Chu J.H., Yu L., Cui W.C., Bi A.D., Huang J., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Huang C., Wu T., Xu S., Gu W., Wang Y., Han Z., Chen Z. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hematopoietic stem cell.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary gland.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Crystal structure of human RAB4B in complex with GDP." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 6-183 IN COMPLEX WITH GDP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF087861 mRNA. Translation: AAP97171.1.
AF165522 mRNA. Translation: AAD45923.1.
AF217985 mRNA. Translation: AAG17228.1.
AF498935 mRNA. Translation: AAM21083.1.
BC046927 mRNA. Translation: AAH46927.1.

IPI

IPI00187143.
IPI00477489.

RefSeq

NP_057238.3. NM_016154.4.

UniGene

Hs.631539.
Hs.730737.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O52	X-ray	2.20	A/B	6-183	[»]

ProteinModelPortal

P61018.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000349560.

PTM databases

PhosphoSite

P61018.

Polymorphism databases

DMDM

46577635.

Proteomic databases

PaxDb

P61018.

PRIDE

P61018.

Protocols and materials databases

DNASU

53916.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000357052; ENSP00000349560; ENSG00000167578.
ENST00000594800; ENSP00000470246; ENSG00000167578.

GeneID

53916.

KEGG

hsa:53916.

UCSC

uc002opd.2. human.
uc002opf.2. human.

Organism-specific databases

CTD

53916.

GeneCards

GC19P041284.

HGNC

HGNC:9782. RAB4B.

MIM

612945. gene.

neXtProt

NX_P61018.

PharmGKB

PA34142.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

InParanoid

P61018.

KO

K07880.

OMA

SPNIVIL.

OrthoDB

EOG4WM4VM.

Gene expression databases

ArrayExpress

P61018.

Bgee

P61018.

CleanEx

HS_RAB4B.

Genevestigator

P61018.

GermOnline

ENSG00000167578. Homo sapiens.

Family and domain databases

InterPro

IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

SUPFAM

SSF52540. SSF52540. 1 hit.

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P61018.

GenomeRNAi

53916.

NextBio

56222.

SOURCE

Search...

Entry information

Entry name

RAB4B_HUMAN

Accession

Primary (citable) accession number: P61018
Secondary accession number(s): P22750, Q7Z514, Q9HBR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.