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Protein

Cardiac phospholamban

Gene

Pln

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity

GO - Molecular functioni

  • ATPase inhibitor activity Source: RGD
  • calcium channel regulator activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiac phospholamban
Short name:
PLB
Gene namesi
Name:Pln
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi619894. Pln.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • calcium ion-transporting ATPase complex Source: Ensembl
  • mitochondrial membrane Source: UniProtKB-SubCell
  • protein complex Source: RGD
  • sarcoplasmic reticulum Source: RGD
  • sarcoplasmic reticulum membrane Source: UniProtKB
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Cardiac phospholambanPRO_0000191248Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei16 – 161PhosphoserineCombined sources
Modified residuei17 – 171PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP61016.
PRIDEiP61016.

PTM databases

iPTMnetiP61016.
PhosphoSiteiP61016.

Expressioni

Tissue specificityi

Heart.1 Publication

Gene expression databases

GenevisibleiP61016. RN.

Interactioni

Subunit structurei

Homopentamer. Interacts with HAX1 and ATP2A2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap7Q6JP776EBI-7620725,EBI-6096191

Protein-protein interaction databases

IntActiP61016. 1 interaction.
MINTiMINT-5227067.
STRINGi10116.ENSRNOP00000000469.

Structurei

3D structure databases

ProteinModelPortaliP61016.
SMRiP61016. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholamban family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
GeneTreeiENSGT00390000002403.
HOGENOMiHOG000115660.
HOVERGENiHBG049447.
InParanoidiP61016.
KOiK05852.
OMAiMERVQHM.
TreeFamiTF330750.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.

Sequencei

Sequence statusi: Complete.

P61016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM

LL
Length:52
Mass (Da):6,095
Last modified:April 26, 2004 - v1
Checksum:i0763601F76A854D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03382 Genomic DNA. Translation: AAA41849.1.
X71068 mRNA. Translation: CAA50394.1.
S95849 mRNA. Translation: AAN86727.1.
S95853 mRNA. Translation: AAB21903.1.
PIRiS37638.
RefSeqiNP_073198.1. NM_022707.2.
UniGeneiRn.9740.

Genome annotation databases

EnsembliENSRNOT00000000469; ENSRNOP00000000469; ENSRNOG00000000413.
GeneIDi64672.
KEGGirno:64672.
UCSCiRGD:619894. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03382 Genomic DNA. Translation: AAA41849.1.
X71068 mRNA. Translation: CAA50394.1.
S95849 mRNA. Translation: AAN86727.1.
S95853 mRNA. Translation: AAB21903.1.
PIRiS37638.
RefSeqiNP_073198.1. NM_022707.2.
UniGeneiRn.9740.

3D structure databases

ProteinModelPortaliP61016.
SMRiP61016. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP61016. 1 interaction.
MINTiMINT-5227067.
STRINGi10116.ENSRNOP00000000469.

PTM databases

iPTMnetiP61016.
PhosphoSiteiP61016.

Proteomic databases

PaxDbiP61016.
PRIDEiP61016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000469; ENSRNOP00000000469; ENSRNOG00000000413.
GeneIDi64672.
KEGGirno:64672.
UCSCiRGD:619894. rat.

Organism-specific databases

CTDi5350.
RGDi619894. Pln.

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
GeneTreeiENSGT00390000002403.
HOGENOMiHOG000115660.
HOVERGENiHBG049447.
InParanoidiP61016.
KOiK05852.
OMAiMERVQHM.
TreeFamiTF330750.

Enzyme and pathway databases

ReactomeiR-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP61016.

Gene expression databases

GenevisibleiP61016. RN.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a highly conserved region at the 5' flank of the phospholamban gene."
    Johns D.C., Feldman A.M.
    Biochem. Biophys. Res. Commun. 188:927-933(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation of gene markers of differentiated and proliferating vascular smooth muscle cells."
    Shanahan C.M., Weissberg P.L., Metcalfe J.C.
    Circ. Res. 73:193-204(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning phospholamban cDNA from rat aortic smooth muscle."
    Hwang K.S., Nadal-Ginard B.
    Adv. Exp. Med. Biol. 304:387-395(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Aortic smooth muscle.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPLA_RAT
AccessioniPrimary (citable) accession number: P61016
Secondary accession number(s): P20006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.