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P61015

- PPLA_RABIT

UniProt

P61015 - PPLA_RABIT

Protein

Cardiac phospholamban

Gene

PLN

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation By similarity.By similarity

    GO - Molecular functioni

    1. ATPase binding Source: BHF-UCL
    2. ATPase inhibitor activity Source: BHF-UCL
    3. calcium channel regulator activity Source: InterPro
    4. enzyme inhibitor activity Source: BHF-UCL
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. adrenergic receptor signaling pathway involved in heart process Source: Ensembl
    2. calcium ion transport Source: InterPro
    3. cardiac muscle tissue development Source: Ensembl
    4. cellular calcium ion homeostasis Source: Ensembl
    5. negative regulation of calcium ion binding Source: BHF-UCL
    6. negative regulation of calcium ion import Source: BHF-UCL
    7. negative regulation of calcium ion transmembrane transporter activity Source: BHF-UCL
    8. negative regulation of calcium-transporting ATPase activity Source: Ensembl
    9. negative regulation of catalytic activity Source: BHF-UCL
    10. negative regulation of heart rate Source: Ensembl
    11. regulation of calcium ion transport Source: UniProtKB
    12. regulation of cardiac muscle cell contraction Source: Ensembl
    13. regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: Ensembl
    14. regulation of relaxation of muscle Source: Ensembl
    15. regulation of ryanodine-sensitive calcium-release channel activity Source: Ensembl
    16. regulation of the force of heart contraction by cardiac conduction Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cardiac phospholamban
    Short name:
    PLB
    Gene namesi
    Name:PLN
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. calcium ion-transporting ATPase complex Source: Ensembl
    2. endoplasmic reticulum Source: BHF-UCL
    3. mitochondrial membrane Source: UniProtKB-SubCell
    4. perinuclear region of cytoplasm Source: BHF-UCL
    5. sarcoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5252Cardiac phospholambanPRO_0000191247Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei16 – 161Phosphoserine; by PKA and DMPKBy similarity
    Modified residuei17 – 171Phosphothreonine; by CaMK2By similarity

    Post-translational modificationi

    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Expressioni

    Tissue specificityi

    Heart.

    Interactioni

    Subunit structurei

    Homopentamer. Interacts with HAX1 and ATP2A2 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-31022N.
    IntActiP61015. 1 interaction.

    Structurei

    Secondary structure

    1
    52
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 1615
    Helixi22 – 5029

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N7LNMR-A1-52[»]
    1XNUmodel-A/B/C/D/E1-52[»]
    2KB7Other-P1-52[»]
    2KYVOther-A/B/C/D/E1-52[»]
    2LPFNMR-A1-52[»]
    2M3BOther-A/B/C/D/E1-52[»]
    ProteinModelPortaliP61015.
    SMRiP61015. Positions 1-52.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP61015.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phospholamban family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG44917.
    HOVERGENiHBG049447.
    OMAiESINNEY.

    Family and domain databases

    InterProiIPR005984. P_lamban.
    [Graphical view]
    PANTHERiPTHR21194. PTHR21194. 1 hit.
    PfamiPF04272. Phospholamban. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001665. PLB. 1 hit.
    ProDomiPD014689. P_lamban. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    TIGRFAMsiTIGR01294. P_lamban. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P61015-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM   50
    LL 52
    Length:52
    Mass (Da):6,095
    Last modified:April 26, 2004 - v1
    Checksum:i0763601F76A854D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63600 Genomic DNA. No translation available.
    M63601 Genomic DNA. Translation: AAA31445.1.
    Y00761 mRNA. Translation: CAA68730.1.
    PIRiB40424.
    RefSeqiNP_001076090.1. NM_001082621.1.
    UniGeneiOcu.3266.

    Genome annotation databases

    GeneIDi100009299.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63600 Genomic DNA. No translation available.
    M63601 Genomic DNA. Translation: AAA31445.1 .
    Y00761 mRNA. Translation: CAA68730.1 .
    PIRi B40424.
    RefSeqi NP_001076090.1. NM_001082621.1.
    UniGenei Ocu.3266.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N7L NMR - A 1-52 [» ]
    1XNU model - A/B/C/D/E 1-52 [» ]
    2KB7 Other - P 1-52 [» ]
    2KYV Other - A/B/C/D/E 1-52 [» ]
    2LPF NMR - A 1-52 [» ]
    2M3B Other - A/B/C/D/E 1-52 [» ]
    ProteinModelPortali P61015.
    SMRi P61015. Positions 1-52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-31022N.
    IntActi P61015. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009299.

    Organism-specific databases

    CTDi 5350.

    Phylogenomic databases

    eggNOGi NOG44917.
    HOVERGENi HBG049447.
    OMAi ESINNEY.

    Miscellaneous databases

    EvolutionaryTracei P61015.
    NextBioi 295180.

    Family and domain databases

    InterProi IPR005984. P_lamban.
    [Graphical view ]
    PANTHERi PTHR21194. PTHR21194. 1 hit.
    Pfami PF04272. Phospholamban. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001665. PLB. 1 hit.
    ProDomi PD014689. P_lamban. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    TIGRFAMsi TIGR01294. P_lamban. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6."
      Fujii J., Zarain-Herzberg A., Willard H.F., Tada M., Maclennan D.H.
      J. Biol. Chem. 266:11669-11675(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Rabbit cardiac and slow-twitch muscle express the same phospholamban gene."
      Fujii J., Lytton J., Tada M., Maclennan D.H.
      FEBS Lett. 227:51-55(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "NMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micelles."
      Zamoon J., Mascioni A., Thomas D.D., Veglia G.
      Biophys. J. 85:2589-2598(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    4. "Phospholamban pentamer quaternary conformation determined by in-gel fluorescence anisotropy."
      Robia S.L., Flohr N.C., Thomas D.D.
      Biochemistry 44:4302-4311(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiPPLA_RABIT
    AccessioniPrimary (citable) accession number: P61015
    Secondary accession number(s): P20006
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3