P61015 (PPLA_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cardiac phospholamban
Short name=PLB

Gene names

Name:

PLN

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	52 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
Subunit structure	Homopentamer. Interacts with HAX1 By similarity.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein. Sarcoplasmic reticulum By similarity.
Tissue specificity	Heart.
Post-translational modification	Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes By similarity.
Sequence similarities	Belongs to the phospholamban family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Sarcoplasmic reticulum
Domain	Transmembrane Transmembrane helix
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adrenergic receptor signaling pathway involved in heart process Inferred from electronic annotation. Source: Compara calcium ion transport Inferred from electronic annotation. Source: InterPro cardiac muscle tissue development Inferred from electronic annotation. Source: Compara cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara negative regulation of calcium ion binding Inferred from direct assay PubMed 12032137PubMed 16432188. Source: BHF-UCL negative regulation of calcium ion import Inferred from direct assay PubMed 12032137. Source: BHF-UCL negative regulation of calcium ion transmembrane transporter activity Inferred from direct assay PubMed 12032137. Source: BHF-UCL negative regulation of heart contraction Inferred from electronic annotation. Source: Compara regulation of cardiac muscle cell contraction Inferred from electronic annotation. Source: Compara regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Inferred from electronic annotation. Source: Compara regulation of relaxation of muscle Inferred from electronic annotation. Source: Compara regulation of ryanodine-sensitive calcium-release channel activity Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction by cardiac conduction Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 16432188. Source: BHF-UCL integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from direct assay PubMed 16432188. Source: BHF-UCL sarcoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATPase binding Inferred from direct assay PubMed 12032137. Source: BHF-UCL ATPase inhibitor activity Inferred by curator PubMed 16432188. Source: BHF-UCL calcium channel regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 52

52

Cardiac phospholamban

PRO_0000191247

Regions

Topological domain

1 – 31

31

Cytoplasmic Potential

Transmembrane

32 – 52

21

Helical; Potential

Amino acid modifications

Modified residue

1

1

N-acetylmethionine By similarity

Modified residue

16

1

Phosphoserine; by PKA and DMPK By similarity

Modified residue

17

1

Phosphothreonine; by CaMK2 By similarity

Secondary structure

1

.

.

.

.

.

52

Helix Strand Turn

Details...

Helix

2 – 16

15

Helix

22 – 50

29

Sequences

	Sequence		Length	Mass (Da)
	P61015 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: 0763601F76A854D3	FASTA	52	6,095
	10 20 30 40 50 MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM LL

References

[1]	"Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6." Fujii J., Zarain-Herzberg A., Willard H.F., Tada M., Maclennan D.H. J. Biol. Chem. 266:11669-11675(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Rabbit cardiac and slow-twitch muscle express the same phospholamban gene." Fujii J., Lytton J., Tada M., Maclennan D.H. FEBS Lett. 227:51-55(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"NMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micelles." Zamoon J., Mascioni A., Thomas D.D., Veglia G. Biophys. J. 85:2589-2598(2003) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[4]	"Phospholamban pentamer quaternary conformation determined by in-gel fluorescence anisotropy." Robia S.L., Flohr N.C., Thomas D.D. Biochemistry 44:4302-4311(2005) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M63600 Genomic DNA. No translation available.
M63601 Genomic DNA. Translation: AAA31445.1.
Y00761 mRNA. Translation: CAA68730.1.

PIR

B40424.

RefSeq

NP_001076090.1. NM_001082621.1.

UniGene

Ocu.3266.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N7L	NMR	-	A	1-52	[»]
1XNU	model	-	A/B/C/D/E	1-52	[»]
2LPF	NMR	-	A	1-52	[»]

ProteinModelPortal

P61015.

SMR

P61015. Positions 1-52.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31022N.

IntAct

P61015. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

100009299.

Organism-specific databases

CTD

5350.

Phylogenomic databases

eggNOG

NOG44917.

HOVERGEN

HBG049447.

OMA

QHMTRSA.

Family and domain databases

InterPro

IPR005984. P_lamban.
[Graphical view]

PANTHER

PTHR21194. PTHR21194. 1 hit.

Pfam

PF04272. Phospholamban. 1 hit.
[Graphical view]

PIRSF

PIRSF001665. PLB. 1 hit.

ProDom

PD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01294. P_lamban. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P61015.

NextBio

295180.

Entry information

Entry name

PPLA_RABIT

Accession

Primary (citable) accession number: P61015
Secondary accession number(s): P20006

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program