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Protein

Cardiac phospholamban

Gene

PLN

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL
  • ATPase inhibitor activity Source: BHF-UCL
  • calcium channel regulator activity Source: InterPro
  • enzyme inhibitor activity Source: BHF-UCL

GO - Biological processi

  • calcium ion transport Source: InterPro
  • negative regulation of calcium ion binding Source: BHF-UCL
  • negative regulation of calcium ion import Source: BHF-UCL
  • negative regulation of calcium ion transmembrane transporter activity Source: BHF-UCL
  • negative regulation of catalytic activity Source: BHF-UCL
  • regulation of calcium ion transport Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiac phospholamban
Short name:
PLB
Gene namesi
Name:PLN
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: BHF-UCL
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial membrane Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: BHF-UCL
  • sarcoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Cardiac phospholambanPRO_0000191247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei16 – 161Phosphoserine; by PKA and DMPKBy similarity
Modified residuei17 – 171Phosphothreonine; by CaMK2By similarity

Post-translational modificationi

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

PTM databases

iPTMnetiP61015.

Expressioni

Tissue specificityi

Heart.1 Publication

Interactioni

Subunit structurei

Homopentamer (By similarity). Interacts with HAX1 and ATP2A2 (By similarity).By similarity

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-31022N.
IntActiP61015. 1 interaction.
STRINGi9986.ENSOCUP00000011917.

Structurei

Secondary structure

1
52
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1615Combined sources
Helixi22 – 5029Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N7LNMR-A1-52[»]
1XNUmodel-A/B/C/D/E1-52[»]
2KB7Other-P1-52[»]
2KYVOther-A/B/C/D/E1-52[»]
2LPFNMR-A1-52[»]
2M3BOther-A/B/C/D/E1-52[»]
ProteinModelPortaliP61015.
SMRiP61015. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61015.

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholamban family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
HOVERGENiHBG049447.
InParanoidiP61015.
KOiK05852.
OMAiMERVQHM.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.

Sequencei

Sequence statusi: Complete.

P61015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM

LL
Length:52
Mass (Da):6,095
Last modified:April 26, 2004 - v1
Checksum:i0763601F76A854D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63600 Genomic DNA. No translation available.
M63601 Genomic DNA. Translation: AAA31445.1.
Y00761 mRNA. Translation: CAA68730.1.
PIRiB40424.
RefSeqiNP_001076090.1. NM_001082621.2.
UniGeneiOcu.3266.

Genome annotation databases

GeneIDi100009299.
KEGGiocu:100009299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63600 Genomic DNA. No translation available.
M63601 Genomic DNA. Translation: AAA31445.1.
Y00761 mRNA. Translation: CAA68730.1.
PIRiB40424.
RefSeqiNP_001076090.1. NM_001082621.2.
UniGeneiOcu.3266.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N7LNMR-A1-52[»]
1XNUmodel-A/B/C/D/E1-52[»]
2KB7Other-P1-52[»]
2KYVOther-A/B/C/D/E1-52[»]
2LPFNMR-A1-52[»]
2M3BOther-A/B/C/D/E1-52[»]
ProteinModelPortaliP61015.
SMRiP61015. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-31022N.
IntActiP61015. 1 interaction.
STRINGi9986.ENSOCUP00000011917.

PTM databases

iPTMnetiP61015.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009299.
KEGGiocu:100009299.

Organism-specific databases

CTDi5350.

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
HOVERGENiHBG049447.
InParanoidiP61015.
KOiK05852.
OMAiMERVQHM.

Miscellaneous databases

EvolutionaryTraceiP61015.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPLA_RABIT
AccessioniPrimary (citable) accession number: P61015
Secondary accession number(s): P20006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.