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P61015

- PPLA_RABIT

UniProt

P61015 - PPLA_RABIT

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Protein

Cardiac phospholamban

Gene

PLN

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity

GO - Molecular functioni

  1. ATPase binding Source: BHF-UCL
  2. ATPase inhibitor activity Source: BHF-UCL
  3. calcium channel regulator activity Source: InterPro
  4. enzyme inhibitor activity Source: BHF-UCL

GO - Biological processi

  1. adrenergic receptor signaling pathway involved in heart process Source: Ensembl
  2. calcium ion transport Source: InterPro
  3. cardiac muscle tissue development Source: Ensembl
  4. cellular calcium ion homeostasis Source: Ensembl
  5. negative regulation of calcium ion binding Source: BHF-UCL
  6. negative regulation of calcium ion import Source: BHF-UCL
  7. negative regulation of calcium ion transmembrane transporter activity Source: BHF-UCL
  8. negative regulation of calcium-transporting ATPase activity Source: Ensembl
  9. negative regulation of catalytic activity Source: BHF-UCL
  10. negative regulation of heart rate Source: Ensembl
  11. regulation of calcium ion transport Source: UniProtKB
  12. regulation of cardiac muscle cell contraction Source: Ensembl
  13. regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: Ensembl
  14. regulation of relaxation of muscle Source: Ensembl
  15. regulation of ryanodine-sensitive calcium-release channel activity Source: Ensembl
  16. regulation of the force of heart contraction by cardiac conduction Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiac phospholamban
Short name:
PLB
Gene namesi
Name:PLN
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei32 – 5221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. calcium ion-transporting ATPase complex Source: Ensembl
  2. endoplasmic reticulum Source: BHF-UCL
  3. mitochondrion Source: UniProtKB-KW
  4. perinuclear region of cytoplasm Source: BHF-UCL
  5. sarcoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Cardiac phospholambanPRO_0000191247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei16 – 161Phosphoserine; by PKA and DMPKBy similarity
Modified residuei17 – 171Phosphothreonine; by CaMK2By similarity

Post-translational modificationi

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Expressioni

Tissue specificityi

Heart.

Interactioni

Subunit structurei

Homopentamer. Interacts with HAX1 and ATP2A2 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-31022N.
IntActiP61015. 1 interaction.

Structurei

Secondary structure

1
52
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1615Combined sources
Helixi22 – 5029Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N7LNMR-A1-52[»]
1XNUmodel-A/B/C/D/E1-52[»]
2KB7Other-P1-52[»]
2KYVOther-A/B/C/D/E1-52[»]
2LPFNMR-A1-52[»]
2M3BOther-A/B/C/D/E1-52[»]
ProteinModelPortaliP61015.
SMRiP61015. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61015.

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholamban family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG44917.
HOVERGENiHBG049447.
InParanoidiP61015.
OMAiESINNEY.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.

Sequencei

Sequence statusi: Complete.

P61015-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM

LL
Length:52
Mass (Da):6,095
Last modified:April 26, 2004 - v1
Checksum:i0763601F76A854D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63600 Genomic DNA. No translation available.
M63601 Genomic DNA. Translation: AAA31445.1.
Y00761 mRNA. Translation: CAA68730.1.
PIRiB40424.
RefSeqiNP_001076090.1. NM_001082621.1.
UniGeneiOcu.3266.

Genome annotation databases

GeneIDi100009299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63600 Genomic DNA. No translation available.
M63601 Genomic DNA. Translation: AAA31445.1 .
Y00761 mRNA. Translation: CAA68730.1 .
PIRi B40424.
RefSeqi NP_001076090.1. NM_001082621.1.
UniGenei Ocu.3266.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1N7L NMR - A 1-52 [» ]
1XNU model - A/B/C/D/E 1-52 [» ]
2KB7 Other - P 1-52 [» ]
2KYV Other - A/B/C/D/E 1-52 [» ]
2LPF NMR - A 1-52 [» ]
2M3B Other - A/B/C/D/E 1-52 [» ]
ProteinModelPortali P61015.
SMRi P61015. Positions 1-52.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-31022N.
IntActi P61015. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009299.

Organism-specific databases

CTDi 5350.

Phylogenomic databases

eggNOGi NOG44917.
HOVERGENi HBG049447.
InParanoidi P61015.
OMAi ESINNEY.

Miscellaneous databases

EvolutionaryTracei P61015.
NextBioi 295180.

Family and domain databases

InterProi IPR005984. P_lamban.
[Graphical view ]
PANTHERi PTHR21194. PTHR21194. 1 hit.
Pfami PF04272. Phospholamban. 1 hit.
[Graphical view ]
PIRSFi PIRSF001665. PLB. 1 hit.
ProDomi PD014689. P_lamban. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
TIGRFAMsi TIGR01294. P_lamban. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6."
    Fujii J., Zarain-Herzberg A., Willard H.F., Tada M., Maclennan D.H.
    J. Biol. Chem. 266:11669-11675(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Rabbit cardiac and slow-twitch muscle express the same phospholamban gene."
    Fujii J., Lytton J., Tada M., Maclennan D.H.
    FEBS Lett. 227:51-55(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "NMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micelles."
    Zamoon J., Mascioni A., Thomas D.D., Veglia G.
    Biophys. J. 85:2589-2598(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  4. "Phospholamban pentamer quaternary conformation determined by in-gel fluorescence anisotropy."
    Robia S.L., Flohr N.C., Thomas D.D.
    Biochemistry 44:4302-4311(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiPPLA_RABIT
AccessioniPrimary (citable) accession number: P61015
Secondary accession number(s): P20006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3