Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P61014

- PPLA_MOUSE

UniProt

P61014 - PPLA_MOUSE

Protein

Cardiac phospholamban

Gene

Pln

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.1 Publication

    GO - Molecular functioni

    1. ATPase inhibitor activity Source: Ensembl
    2. calcium channel regulator activity Source: InterPro
    3. protein binding Source: MGI

    GO - Biological processi

    1. adrenergic receptor signaling pathway involved in heart process Source: MGI
    2. calcium ion transport Source: Ensembl
    3. cardiac muscle tissue development Source: MGI
    4. cellular calcium ion homeostasis Source: MGI
    5. negative regulation of calcium ion transport Source: Ensembl
    6. negative regulation of calcium-transporting ATPase activity Source: Ensembl
    7. negative regulation of heart contraction Source: MGI
    8. negative regulation of heart rate Source: Ensembl
    9. protein homooligomerization Source: Ensembl
    10. regulation of calcium ion import Source: MGI
    11. regulation of calcium ion transport Source: UniProtKB
    12. regulation of cardiac muscle cell contraction Source: MGI
    13. regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: MGI
    14. regulation of heart contraction Source: MGI
    15. regulation of relaxation of muscle Source: MGI
    16. regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: MGI
    17. regulation of ryanodine-sensitive calcium-release channel activity Source: MGI
    18. regulation of the force of heart contraction Source: MGI
    19. regulation of the force of heart contraction by cardiac conduction Source: MGI
    20. response to testosterone Source: Ensembl
    21. response to zinc ion Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cardiac phospholamban
    Short name:
    PLB
    Gene namesi
    Name:Pln
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:97622. Pln.

    Subcellular locationi

    Sarcoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Mitochondrion membrane By similarity; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. calcium ion-transporting ATPase complex Source: Ensembl
    2. mitochondrial membrane Source: UniProtKB-SubCell
    3. sarcoplasmic reticulum Source: MGI
    4. sarcoplasmic reticulum membrane Source: UniProtKB-SubCell
    5. vesicle Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi20 – 223MPQ → GGG: No effect on inhibition of ATP2A1-mediated calcium uptake.
    Mutagenesisi20 – 212MP → GG: Nearly abolishes inhibition of ATP2A1-mediated calcium uptake.
    Mutagenesisi21 – 211P → G: Reduces inhibition of ATP2A1-mediated calcium uptake. 1 Publication
    Mutagenesisi22 – 221Q → G: Strongly reduces inhibition of ATP2A1-mediated calcium uptake. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5252Cardiac phospholambanPRO_0000191245Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei16 – 161Phosphoserine; by DMPK; alternateBy similarity
    Modified residuei16 – 161Phosphoserine; by PKA; alternate2 Publications
    Modified residuei17 – 171Phosphothreonine; by CaMK2By similarity

    Post-translational modificationi

    Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes By similarity. Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake.By similarity2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP61014.
    PRIDEiP61014.

    PTM databases

    PhosphoSiteiP61014.

    Expressioni

    Gene expression databases

    BgeeiP61014.
    CleanExiMM_PLN.
    GenevestigatoriP61014.

    Interactioni

    Subunit structurei

    Homopentamer. Interacts with HAX1 and ATP2A2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202252. 1 interaction.
    STRINGi10090.ENSMUSP00000045709.

    Structurei

    Secondary structure

    1
    52
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni9 – 113

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3O7LX-ray2.80I4-18[»]
    ProteinModelPortaliP61014.
    SMRiP61014. Positions 1-52.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phospholamban family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG44917.
    GeneTreeiENSGT00390000002403.
    HOGENOMiHOG000115660.
    HOVERGENiHBG049447.
    InParanoidiP61014.
    KOiK05852.
    OMAiQHARQNL.
    TreeFamiTF330750.

    Family and domain databases

    InterProiIPR005984. P_lamban.
    [Graphical view]
    PANTHERiPTHR21194. PTHR21194. 1 hit.
    PfamiPF04272. Phospholamban. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001665. PLB. 1 hit.
    ProDomiPD014689. P_lamban. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    TIGRFAMsiTIGR01294. P_lamban. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P61014-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM   50
    LL 52
    Length:52
    Mass (Da):6,095
    Last modified:April 26, 2004 - v1
    Checksum:i0763601F76A854D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S46792 mRNA. Translation: AAB23706.1.
    AK002622 mRNA. Translation: BAB22237.1.
    AK040718 mRNA. Translation: BAC30680.1.
    AK052199 mRNA. Translation: BAC34880.1.
    AK142708 mRNA. Translation: BAE25168.1.
    BC061097 mRNA. Translation: AAH61097.1.
    CCDSiCCDS35899.1.
    PIRiA49057.
    RefSeqiNP_001135399.1. NM_001141927.1.
    NP_075618.1. NM_023129.5.
    UniGeneiMm.34145.

    Genome annotation databases

    EnsembliENSMUST00000046221; ENSMUSP00000045709; ENSMUSG00000038583.
    ENSMUST00000163319; ENSMUSP00000132743; ENSMUSG00000038583.
    GeneIDi18821.
    KEGGimmu:18821.
    UCSCiuc007fbo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S46792 mRNA. Translation: AAB23706.1 .
    AK002622 mRNA. Translation: BAB22237.1 .
    AK040718 mRNA. Translation: BAC30680.1 .
    AK052199 mRNA. Translation: BAC34880.1 .
    AK142708 mRNA. Translation: BAE25168.1 .
    BC061097 mRNA. Translation: AAH61097.1 .
    CCDSi CCDS35899.1.
    PIRi A49057.
    RefSeqi NP_001135399.1. NM_001141927.1.
    NP_075618.1. NM_023129.5.
    UniGenei Mm.34145.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3O7L X-ray 2.80 I 4-18 [» ]
    ProteinModelPortali P61014.
    SMRi P61014. Positions 1-52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202252. 1 interaction.
    STRINGi 10090.ENSMUSP00000045709.

    PTM databases

    PhosphoSitei P61014.

    Proteomic databases

    PaxDbi P61014.
    PRIDEi P61014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046221 ; ENSMUSP00000045709 ; ENSMUSG00000038583 .
    ENSMUST00000163319 ; ENSMUSP00000132743 ; ENSMUSG00000038583 .
    GeneIDi 18821.
    KEGGi mmu:18821.
    UCSCi uc007fbo.2. mouse.

    Organism-specific databases

    CTDi 5350.
    MGIi MGI:97622. Pln.

    Phylogenomic databases

    eggNOGi NOG44917.
    GeneTreei ENSGT00390000002403.
    HOGENOMi HOG000115660.
    HOVERGENi HBG049447.
    InParanoidi P61014.
    KOi K05852.
    OMAi QHARQNL.
    TreeFami TF330750.

    Miscellaneous databases

    NextBioi 295180.
    PROi P61014.
    SOURCEi Search...

    Gene expression databases

    Bgeei P61014.
    CleanExi MM_PLN.
    Genevestigatori P61014.

    Family and domain databases

    InterProi IPR005984. P_lamban.
    [Graphical view ]
    PANTHERi PTHR21194. PTHR21194. 1 hit.
    Pfami PF04272. Phospholamban. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001665. PLB. 1 hit.
    ProDomi PD014689. P_lamban. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    TIGRFAMsi TIGR01294. P_lamban. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse phospholamban gene expression during development in vivo and in vitro."
      Ganim J.R., Luo W., Ponniah S., Grupp I., Kim H.W., Ferguson D.G., Kadambi V., Neumann J.C., Doetschman T., Kranias E.G.
      Circ. Res. 71:1021-1030(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Aorta, Heart and Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. "Tuning the structural coupling between the transmembrane and cytoplasmic domains of phospholamban to control sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) function."
      Ha K.N., Gustavsson M., Veglia G.
      J. Muscle Res. Cell Motil. 33:485-492(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF 20-MET--GLN-22; PRO-21 AND GLN-22.
    5. "Dynamics connect substrate recognition to catalysis in protein kinase A."
      Masterson L.R., Cheng C., Yu T., Tonelli M., Kornev A., Taylor S.S., Veglia G.
      Nat. Chem. Biol. 6:821-828(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 4-18 IN COMPLEX WITH PRKACA, PHOSPHORYLATION AT SER-16, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPLA_MOUSE
    AccessioniPrimary (citable) accession number: P61014
    Secondary accession number(s): P20006, Q3UQ75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3