P61014 (PPLA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cardiac phospholamban
Short name=PLB

Gene names

Name:

Pln

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	52 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
Subunit structure	Homopentamer. Interacts with HAX1 By similarity.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein. Sarcoplasmic reticulum By similarity.
Post-translational modification	Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes By similarity.
Sequence similarities	Belongs to the phospholamban family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Sarcoplasmic reticulum
Domain	Transmembrane Transmembrane helix
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adrenergic receptor signaling pathway involved in heart process Inferred from mutant phenotype PubMed 10471356. Source: MGI calcium ion transport Inferred from electronic annotation. Source: Compara cardiac muscle tissue development Inferred from genetic interaction PubMed 10555147. Source: MGI cellular calcium ion homeostasis Inferred from genetic interaction PubMed 10555147. Source: MGI negative regulation of heart contraction Inferred from mutant phenotype PubMed 8062415. Source: MGI regulation of calcium ion import Inferred from mutant phenotype PubMed 8620604. Source: MGI regulation of cardiac muscle cell contraction Inferred from mutant phenotype PubMed 8620604. Source: MGI regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Inferred from mutant phenotype PubMed 8831698. Source: MGI regulation of relaxation of muscle Inferred from mutant phenotype PubMed 10024311. Source: MGI regulation of ryanodine-sensitive calcium-release channel activity Inferred from mutant phenotype PubMed 10603936. Source: MGI regulation of the force of heart contraction by cardiac conduction Inferred from mutant phenotype PubMed 10644605. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from electronic annotation. Source: Compara sarcoplasmic reticulum Inferred from direct assay PubMed 9038922. Source: MGI vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	ATPase inhibitor activity Inferred from electronic annotation. Source: Compara calcium channel regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 52

52

Cardiac phospholamban

PRO_0000191245

Regions

Topological domain

1 – 31

31

Cytoplasmic Potential

Transmembrane

32 – 52

21

Helical; Potential

Amino acid modifications

Modified residue

1

1

N-acetylmethionine By similarity

Modified residue

16

1

Phosphoserine; by PKA and DMPK By similarity

Modified residue

17

1

Phosphothreonine; by CaMK2 By similarity

Secondary structure

1

.

.

.

52

Helix Strand Turn

Details...

Turn

9 – 11

3

Sequences

	Sequence		Length	Mass (Da)
	P61014 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: 0763601F76A854D3	FASTA	52	6,095
	10 20 30 40 50 MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse phospholamban gene expression during development in vivo and in vitro." Ganim J.R., Luo W., Ponniah S., Grupp I., Kim H.W., Ferguson D.G., Kadambi V., Neumann J.C., Doetschman T., Kranias E.G. Circ. Res. 71:1021-1030(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Aorta, Heart and Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S46792 mRNA. Translation: AAB23706.1.
AK002622 mRNA. Translation: BAB22237.1.
AK040718 mRNA. Translation: BAC30680.1.
AK052199 mRNA. Translation: BAC34880.1.
AK142708 mRNA. Translation: BAE25168.1.
BC061097 mRNA. Translation: AAH61097.1.

IPI

IPI00115432.

PIR

A49057.

RefSeq

NP_001135399.1. NM_001141927.1.
NP_075618.1. NM_023129.5.

UniGene

Mm.34145.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3O7L	X-ray	2.80	I	4-18	[»]

ProteinModelPortal

P61014.

SMR

P61014. Positions 1-52.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000045709.

PTM databases

PhosphoSite

P61014.

Proteomic databases

PaxDb

P61014.

PRIDE

P61014.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000046221; ENSMUSP00000045709; ENSMUSG00000038583.
ENSMUST00000163319; ENSMUSP00000132743; ENSMUSG00000038583.

GeneID

18821.

KEGG

mmu:18821.

UCSC

uc007fbo.2. mouse.

Organism-specific databases

CTD

5350.

MGI

MGI:97622. Pln.

Phylogenomic databases

eggNOG

NOG44917.

GeneTree

ENSGT00390000002403.

HOGENOM

HOG000115660.

HOVERGEN

HBG049447.

InParanoid

P61014.

KO

K05852.

OMA

QHMTRSA.

Gene expression databases

Bgee

P61014.

CleanEx

MM_PLN.

Genevestigator

P61014.

GermOnline

ENSMUSG00000038583. Mus musculus.

Family and domain databases

InterPro

IPR005984. P_lamban.
[Graphical view]

PANTHER

PTHR21194. PTHR21194. 1 hit.

Pfam

PF04272. Phospholamban. 1 hit.
[Graphical view]

PIRSF

PIRSF001665. PLB. 1 hit.

ProDom

PD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01294. P_lamban. 1 hit.

ProtoNet

Search...

Other

NextBio

295180.

SOURCE

Search...

Entry information

Entry name

PPLA_MOUSE

Accession

Primary (citable) accession number: P61014
Secondary accession number(s): P20006, Q3UQ75

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program