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Protein

Cardiac phospholamban

Gene

PLN

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-SSC-5578775. Ion homeostasis.
R-SSC-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiac phospholamban
Short name:
PLB
Gene namesi
Name:PLN
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Cardiac phospholambanPRO_0000191246Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei16 – 161Phosphoserine; by PKA and DMPKBy similarity
Modified residuei17 – 171Phosphothreonine; by CaMK2By similarity

Post-translational modificationi

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP61013.

PTM databases

iPTMnetiP61013.

Expressioni

Gene expression databases

GenevisibleiP61013. SS.

Interactioni

Subunit structurei

Homopentamer (By similarity). Interacts with HAX1 and ATP2A2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004587.

Structurei

Secondary structure

1
52
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1613Combined sources
Beta strandi17 – 204Combined sources
Helixi21 – 4929Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FJKNMR-A1-52[»]
1FJPNMR-A1-52[»]
ProteinModelPortaliP61013.
SMRiP61013. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61013.

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholamban family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
GeneTreeiENSGT00390000002403.
HOGENOMiHOG000115660.
HOVERGENiHBG008240.
InParanoidiP61013.
KOiK05852.
OMAiMERVQHM.
TreeFamiTF330750.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.

Sequencei

Sequence statusi: Complete.

P61013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM

LL
Length:52
Mass (Da):6,080
Last modified:April 1, 1988 - v1
Checksum:i076361D9ADC424D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15075 mRNA. Translation: CAA33171.1.
PIRiS05540.
RefSeqiNP_999378.1. NM_214213.1.
UniGeneiSsc.5227.

Genome annotation databases

EnsembliENSSSCT00000004695; ENSSSCP00000004587; ENSSSCG00000004248.
GeneIDi397421.
KEGGissc:397421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15075 mRNA. Translation: CAA33171.1.
PIRiS05540.
RefSeqiNP_999378.1. NM_214213.1.
UniGeneiSsc.5227.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FJKNMR-A1-52[»]
1FJPNMR-A1-52[»]
ProteinModelPortaliP61013.
SMRiP61013. Positions 1-52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004587.

PTM databases

iPTMnetiP61013.

Proteomic databases

PaxDbiP61013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000004695; ENSSSCP00000004587; ENSSSCG00000004248.
GeneIDi397421.
KEGGissc:397421.

Organism-specific databases

CTDi5350.

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
GeneTreeiENSGT00390000002403.
HOGENOMiHOG000115660.
HOVERGENiHBG008240.
InParanoidiP61013.
KOiK05852.
OMAiMERVQHM.
TreeFamiTF330750.

Enzyme and pathway databases

ReactomeiR-SSC-5578775. Ion homeostasis.
R-SSC-936837. Ion transport by P-type ATPases.

Miscellaneous databases

EvolutionaryTraceiP61013.

Gene expression databases

GenevisibleiP61013. SS.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle."
    Verboomen H., Wuytack F., Eggermont J.A., de Jaegere S., Missiaen L., Raeymaekers L., Casteels R.
    Biochem. J. 262:353-356(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Smooth muscle.
  2. "NMR solution structure of phospholamban membrane protein."
    Lamberth S., Griesinger C., Schmid H., Carafoli E., Muenchbach M., Vorherr T., Krebs J.
    Submitted (AUG-2000) to the PDB data bank
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiPPLA_PIG
AccessioniPrimary (citable) accession number: P61013
Secondary accession number(s): P07473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.