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Protein

Cardiac phospholamban

Gene

PLN

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity

GO - Molecular functioni

  • ATPase inhibitor activity Source: BHF-UCL
  • calcium channel regulator activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-CFA-5578775. Ion homeostasis.
R-CFA-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Cardiac phospholamban
Short name:
PLB
Gene namesi
Name:PLN
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001912431 – 52Cardiac phospholambanAdd BLAST52

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei16Phosphoserine; by PKA and DMPK2 Publications1
Modified residuei17Phosphothreonine; by CaMK22 Publications1

Post-translational modificationi

Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity). Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation.By similarity2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP61012.

PTM databases

iPTMnetiP61012.

Expressioni

Tissue specificityi

Heart.1 Publication

Interactioni

Subunit structurei

Homopentamer (PubMed:3753485). Interacts with HAX1 and ATP2A2 (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000036921.

Structurei

Secondary structure

152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 47Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KYTX-ray2.83B/C1-52[»]
4Y3UX-ray3.51B1-50[»]
ProteinModelPortaliP61012.
SMRiP61012.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholamban family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
GeneTreeiENSGT00390000002403.
HOGENOMiHOG000115660.
HOVERGENiHBG008240.
InParanoidiP61012.
KOiK05852.
OMAiMERVQHM.
TreeFamiTF330750.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.

Sequencei

Sequence statusi: Complete.

P61012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM

LL
Length:52
Mass (Da):6,080
Last modified:April 1, 1988 - v1
Checksum:i076361D9ADC424D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16012 mRNA. Translation: AAA30884.1.
Y00399 mRNA. Translation: CAA68461.1.
M35393 mRNA. Translation: AAC41618.1.
AY514751 Genomic DNA. Translation: AAT44582.1.
AY576872 Genomic DNA. Translation: AAT48602.1.
PIRiA29002.
RefSeqiNP_001003332.1. NM_001003332.1.
XP_013972393.1. XM_014116918.1.
UniGeneiCfa.3816.

Genome annotation databases

EnsembliENSCAFT00000044257; ENSCAFP00000036921; ENSCAFG00000029347.
GeneIDi414755.
KEGGicfa:414755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16012 mRNA. Translation: AAA30884.1.
Y00399 mRNA. Translation: CAA68461.1.
M35393 mRNA. Translation: AAC41618.1.
AY514751 Genomic DNA. Translation: AAT44582.1.
AY576872 Genomic DNA. Translation: AAT48602.1.
PIRiA29002.
RefSeqiNP_001003332.1. NM_001003332.1.
XP_013972393.1. XM_014116918.1.
UniGeneiCfa.3816.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KYTX-ray2.83B/C1-52[»]
4Y3UX-ray3.51B1-50[»]
ProteinModelPortaliP61012.
SMRiP61012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000036921.

PTM databases

iPTMnetiP61012.

Proteomic databases

PaxDbiP61012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000044257; ENSCAFP00000036921; ENSCAFG00000029347.
GeneIDi414755.
KEGGicfa:414755.

Organism-specific databases

CTDi5350.

Phylogenomic databases

eggNOGiENOG410J7H0. Eukaryota.
ENOG4111BFT. LUCA.
GeneTreeiENSGT00390000002403.
HOGENOMiHOG000115660.
HOVERGENiHBG008240.
InParanoidiP61012.
KOiK05852.
OMAiMERVQHM.
TreeFamiTF330750.

Enzyme and pathway databases

ReactomeiR-CFA-5578775. Ion homeostasis.
R-CFA-936837. Ion transport by P-type ATPases.

Family and domain databases

InterProiIPR005984. P_lamban.
[Graphical view]
PANTHERiPTHR21194. PTHR21194. 1 hit.
PfamiPF04272. Phospholamban. 1 hit.
[Graphical view]
PIRSFiPIRSF001665. PLB. 1 hit.
ProDomiPD014689. P_lamban. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01294. P_lamban. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPLA_CANLF
AccessioniPrimary (citable) accession number: P61012
Secondary accession number(s): P07473, Q56UH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.