P61011 (SRP54_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle 54 kDa protein
Short name=SRP54

Gene names

Name:

SRP54

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
Subunit structure	Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1. Ref.6
Subcellular location	Nucleus speckle. Cytoplasm Ref.6.
Domain	Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.
Sequence similarities	Belongs to the GTP-binding SRP family. SRP54 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Signal recognition particle
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from direct assay PubMed 8247130. Source: GOC SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Inferred from sequence or structural similarity. Source: UniProtKB SRP-dependent cotranslational protein targeting to membrane, translocation Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from direct assay PubMed 18089836. Source: UniProtKB translation Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay. Source: HPA signal recognition particle, endoplasmic reticulum targeting Inferred from direct assay PubMed 18089836. Source: UniProtKB
Molecular_function	7S RNA binding Inferred from direct assay PubMed 18089836Ref.2. Source: UniProtKB GDP binding Inferred from direct assay PubMed 8622769. Source: UniProtKB GTP binding Inferred from direct assay PubMed 8622769. Source: UniProtKB GTPase activity Inferred from electronic annotation. Source: InterPro drug binding Inferred from direct assay PubMed 18089836. Source: UniProtKB endoplasmic reticulum signal peptide binding Inferred from direct assay Ref.2. Source: UniProtKB ribonucleoprotein complex binding Inferred from direct assay Ref.2. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P61011-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P61011-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVK → MPPLSMKR

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

Signal recognition particle 54 kDa protein

PRO_0000101192

Regions

Nucleotide binding

108 – 115

8

GTP By similarity

Nucleotide binding

190 – 194

5

GTP By similarity

Nucleotide binding

248 – 251

4

GTP By similarity

Region

1 – 295

295

G-domain

Region

296 – 504

209

M-domain

Natural variations

Alternative sequence

1 – 57

57

MVLAD…RENVK → MPPLSMKR in isoform 2.

VSP_043696

Secondary structure

1

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504

Helix Strand Turn

Details...

Helix

329 – 341

13

Helix

343 – 351

9

Turn

357 – 360

4

Helix

365 – 379

15

Helix

384 – 388

5

Turn

390 – 392

3

Helix

393 – 398

6

Helix

401 – 409

9

Helix

414 – 430

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: 7D7630A43BC5EC2C	FASTA	504	55,705
	10 20 30 40 50 60 MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI 70 80 90 100 110 120 DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK QNVIMFVGLQ GSGKTTTCSK 130 140 150 160 170 180 LAYYYQRKGW KTCLICADTF RAGAFDQLKQ NATKARIPFY GSYTEMDPVI IASEGVEKFK 190 200 210 220 230 240 NENFEIIIVD TSGRHKQEDS LFEEMLQVAN AIQPDNIVYV MDASIGQACE AQAKAFKDKV 250 260 270 280 290 300 DVASVIVTKL DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI 310 320 330 340 350 360 EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG MIPGFGTDFM 370 380 390 400 410 420 SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP GRIQRVARGS GVSTRDVQEL 430 440 450 460 470 480 LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL 490 500 QSMMRQFQQG AAGNMKGMMG FNNM
	Isoform 2 [UniParc]. Checksum: 828728336FA34882	FASTA	455	50,369
	10 20 30 40 50 60 MPPLSMKRSA IDLEEMASGL NKRKMIQHAV FKELVKLVDP GVKAWTPTKG KQNVIMFVGL 70 80 90 100 110 120 QGSGKTTTCS KLAYYYQRKG WKTCLICADT FRAGAFDQLK QNATKARIPF YGSYTEMDPV 130 140 150 160 170 180 IIASEGVEKF KNENFEIIIV DTSGRHKQED SLFEEMLQVA NAIQPDNIVY VMDASIGQAC 190 200 210 220 230 240 EAQAKAFKDK VDVASVIVTK LDGHAKGGGA LSAVAATKSP IIFIGTGEHI DDFEPFKTQP 250 260 270 280 290 300 FISKLLGMGD IEGLIDKVNE LKLDDNEALI EKLKHGQFTL RDMYEQFQNI MKMGPFSQIL 310 320 330 340 350 360 GMIPGFGTDF MSKGNEQESM ARLKKLMTIM DSMNDQELDS TDGAKVFSKQ PGRIQRVARG 370 380 390 400 410 420 SGVSTRDVQE LLTQYTKFAQ MVKKMGGIKG LFKGGDMSKN VSQSQMAKLN QQMAKMMDPR 430 440 450 VLHHMGGMAG LQSMMRQFQQ GAAGNMKGMM GFNNM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the highly conserved gene encoding the human 54kDa subunit of signal recognition particle." Patel S., Austen B. DNA Seq. 6:167-170(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[2]	"Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites." Gowda K., Black S.D., Moeller I., Sakakibara Y., Liu M.C., Zwieb C. Gene 207:197-207(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Trachea.
[4]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye and Placenta.
[6]	"Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo." Sakashita E., Tatsumi S., Werner D., Endo H., Mayeda A. Mol. Cell. Biol. 24:1174-1187(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RNPS1, SUBCELLULAR LOCATION.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Crystal structure of the conserved subdomain of human protein SRP54M at 2.1 A resolution: evidence for the mechanism of signal peptide binding." Clemons W.M. Jr., Gowda K., Black S.D., Zwieb C., Ramakrishnan V. J. Mol. Biol. 292:697-705(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 326-432.
[9]	"Induced structural changes of 7SL RNA during the assembly of human signal recognition particle." Kuglstatter A., Oubridge C., Nagai K. Nat. Struct. Biol. 9:740-744(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 323-441 IN COMPLEX WITH SRP19 AND 7SL RNA.
+	Additional computationally mapped references.

Web resources

Wikipedia

Signal recognition particle entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X86373 Genomic DNA. Translation: CAA60132.1.
U51920 mRNA. Translation: AAC50994.1.
AK300824 mRNA. Translation: BAG62478.1.
AK312853 mRNA. Translation: BAG35706.1.
AL049776 Genomic DNA. No translation available.
BC000652 mRNA. Translation: AAH00652.1.
BC003389 mRNA. Translation: AAH03389.1.

IPI

IPI00009822.
IPI00929464.

PIR

S54143.

RefSeq

NP_001139754.1. NM_001146282.1.
NP_003127.1. NM_003136.3.

UniGene

Hs.167535.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MFQ	X-ray	3.10	C	323-441	[»]
1QB2	X-ray	2.10	A/B	326-434	[»]

ProteinModelPortal

P61011.

ModBase

Search...

Protein-protein interaction databases

IntAct

P61011. 1 interaction.

STRING

9606.ENSP00000216774.

Protein family/group databases

TCDB

3.A.5.9.1. general secretory pathway (Sec) family.

PTM databases

PhosphoSite

P61011.

Polymorphism databases

DMDM

46577650.

Proteomic databases

PaxDb

P61011.

PRIDE

P61011.

Protocols and materials databases

DNASU

6729.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000216774; ENSP00000216774; ENSG00000100883.
ENST00000546080; ENSP00000440629; ENSG00000100883.
ENST00000556994; ENSP00000451818; ENSG00000100883.

GeneID

6729.

KEGG

hsa:6729.

UCSC

uc001wso.3. human.

Organism-specific databases

CTD

6729.

GeneCards

GC14P035452.

HGNC

HGNC:11301. SRP54.

HPA

HPA048977.

MIM

604857. gene.

neXtProt

NX_P61011.

PharmGKB

PA36125.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0541.

HOGENOM

HOG000036165.

HOVERGEN

HBG061072.

InParanoid

P61011.

KO

K03106.

OMA

GQQVVKI.

OrthoDB

EOG45MN54.

PhylomeDB

P61011.

Enzyme and pathway databases

Reactome

REACT_17015. Metabolism of proteins.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P61011.

Bgee

P61011.

CleanEx

HS_SRP54.

Genevestigator

P61011.

GermOnline

ENSG00000100883. Homo sapiens.

Family and domain databases

Gene3D

1.10.260.30. 1 hit.

InterPro

IPR003593. AAA+_ATPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]

Pfam

PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]

SUPFAM

SSF47446. Signal_recog_particle_SRP54_M. 1 hit.
SSF47364. SRP54. 1 hit.

TIGRFAMs

TIGR01425. SRP54_euk. 1 hit.

PROSITE

PS00300. SRP54. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SRP54. human.

EvolutionaryTrace

P61011.

GenomeRNAi

6729.

NextBio

26248.

SOURCE

Search...

Entry information

Entry name

SRP54_HUMAN

Accession

Primary (citable) accession number: P61011
Secondary accession number(s): B2R759, B4DUW6, P13624

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.