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Protein

Signal recognition particle 54 kDa protein

Gene

SRP54

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115GTPBy similarity8
Nucleotide bindingi190 – 194GTPBy similarity5
Nucleotide bindingi248 – 251GTPBy similarity4

GO - Molecular functioni

  • 7S RNA binding Source: UniProtKB
  • drug binding Source: UniProtKB
  • endoplasmic reticulum signal peptide binding Source: UniProtKB
  • GDP binding Source: UniProtKB
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB
  • ribonucleoprotein complex binding Source: MGI
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane

Protein family/group databases

TCDBi3.A.5.9.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein
Short name:
SRP54
Gene namesi
Name:SRP54
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100883.11
HGNCiHGNC:11301 SRP54
MIMi604857 gene
neXtProtiNX_P61011

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signal recognition particle

Pathology & Biotechi

Organism-specific databases

DisGeNETi6729
MalaCardsiSRP54
OpenTargetsiENSG00000100883
PharmGKBiPA36125

Polymorphism and mutation databases

BioMutaiSRP54
DMDMi46577650

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011921 – 504Signal recognition particle 54 kDa proteinAdd BLAST504

Proteomic databases

EPDiP61011
MaxQBiP61011
PaxDbiP61011
PeptideAtlasiP61011
PRIDEiP61011
ProteomicsDBi57247
57248 [P61011-2]

PTM databases

CarbonylDBiP61011
iPTMnetiP61011
PhosphoSitePlusiP61011
SwissPalmiP61011

Expressioni

Gene expression databases

BgeeiENSG00000100883
CleanExiHS_SRP54
ExpressionAtlasiP61011 baseline and differential
GenevisibleiP61011 HS

Organism-specific databases

HPAiHPA048977
HPA062044

Interactioni

Subunit structurei

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1.2 Publications

Protein-protein interaction databases

BioGridi112607, 37 interactors
IntActiP61011, 10 interactors
MINTiP61011
STRINGi9606.ENSP00000216774

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 41Combined sources17
Helixi46 – 58Combined sources13
Helixi71 – 74Combined sources4
Helixi76 – 87Combined sources12
Beta strandi102 – 107Combined sources6
Helixi114 – 127Combined sources14
Beta strandi132 – 137Combined sources6
Helixi144 – 154Combined sources11
Helixi168 – 180Combined sources13
Turni181 – 183Combined sources3
Beta strandi185 – 191Combined sources7
Helixi199 – 212Combined sources14
Beta strandi215 – 222Combined sources8
Helixi229 – 239Combined sources11
Beta strandi242 – 248Combined sources7
Helixi258 – 266Combined sources9
Beta strandi270 – 274Combined sources5
Beta strandi276 – 278Combined sources3
Beta strandi282 – 284Combined sources3
Helixi287 – 295Combined sources9
Helixi329 – 341Combined sources13
Helixi343 – 351Combined sources9
Turni357 – 360Combined sources4
Helixi365 – 379Combined sources15
Helixi384 – 388Combined sources5
Turni390 – 392Combined sources3
Helixi393 – 398Combined sources6
Helixi401 – 409Combined sources9
Helixi414 – 430Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MFQX-ray3.10C323-441[»]
1QB2X-ray2.10A/B326-434[»]
5L3QX-ray3.20A/C1-436[»]
ProteinModelPortaliP61011
SMRiP61011
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61011

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 295G-domainAdd BLAST295
Regioni296 – 504M-domainAdd BLAST209

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0780 Eukaryota
COG0541 LUCA
GeneTreeiENSGT00550000074824
HOGENOMiHOG000036165
HOVERGENiHBG061072
InParanoidiP61011
KOiK03106
OMAiDTAGRHK
OrthoDBiEOG091G05OW
PhylomeDBiP61011
TreeFamiTF106249

Family and domain databases

Gene3Di1.10.260.30, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR036225 SRP/SRP_N
IPR022941 SRP54
IPR006325 SRP54_euk
IPR000897 SRP54_GTPase_dom
PANTHERiPTHR11564 PTHR11564, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47364 SSF47364, 1 hit
SSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01425 SRP54_euk, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61011-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK
60 70 80 90 100
QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK
110 120 130 140 150
QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ
160 170 180 190 200
NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS
210 220 230 240 250
LFEEMLQVAN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL
260 270 280 290 300
DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI
310 320 330 340 350
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG
360 370 380 390 400
MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP
410 420 430 440 450
GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV
460 470 480 490 500
SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG

FNNM
Length:504
Mass (Da):55,705
Last modified:April 26, 2004 - v1
Checksum:i7D7630A43BC5EC2C
GO
Isoform 2 (identifier: P61011-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVK → MPPLSMKR

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):50,369
Checksum:i828728336FA34882
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0436961 – 57MVLAD…RENVK → MPPLSMKR in isoform 2. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86373 Genomic DNA Translation: CAA60132.1
U51920 mRNA Translation: AAC50994.1
AK300824 mRNA Translation: BAG62478.1
AK312853 mRNA Translation: BAG35706.1
AL049776 Genomic DNA No translation available.
BC000652 mRNA Translation: AAH00652.1
BC003389 mRNA Translation: AAH03389.1
CCDSiCCDS53893.1 [P61011-2]
CCDS9652.1 [P61011-1]
PIRiS54143
RefSeqiNP_001139754.1, NM_001146282.1 [P61011-2]
NP_003127.1, NM_003136.3 [P61011-1]
XP_011535408.1, XM_011537106.1 [P61011-1]
UniGeneiHs.167535

Genome annotation databases

EnsembliENST00000216774; ENSP00000216774; ENSG00000100883 [P61011-1]
ENST00000546080; ENSP00000440629; ENSG00000100883 [P61011-2]
ENST00000556994; ENSP00000451818; ENSG00000100883 [P61011-1]
GeneIDi6729
KEGGihsa:6729
UCSCiuc001wso.4 human [P61011-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSRP54_HUMAN
AccessioniPrimary (citable) accession number: P61011
Secondary accession number(s): B2R759, B4DUW6, P13624
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: June 20, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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