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P61011

- SRP54_HUMAN

UniProt

P61011 - SRP54_HUMAN

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Protein

Signal recognition particle 54 kDa protein

Gene

SRP54

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158GTPBy similarity
Nucleotide bindingi190 – 1945GTPBy similarity
Nucleotide bindingi248 – 2514GTPBy similarity

GO - Molecular functioni

  1. 7S RNA binding Source: UniProtKB
  2. drug binding Source: UniProtKB
  3. endoplasmic reticulum signal peptide binding Source: UniProtKB
  4. GDP binding Source: UniProtKB
  5. GTPase activity Source: InterPro
  6. GTP binding Source: UniProtKB
  7. poly(A) RNA binding Source: UniProtKB
  8. ribonucleoprotein complex binding Source: MGI

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. gene expression Source: Reactome
  3. GTP catabolic process Source: GOC
  4. protein targeting to ER Source: UniProtKB
  5. response to drug Source: UniProtKB
  6. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  7. SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Source: UniProtKB
  8. SRP-dependent cotranslational protein targeting to membrane, translocation Source: UniProtKB
  9. translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.

Protein family/group databases

TCDBi3.A.5.9.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein
Short name:
SRP54
Gene namesi
Name:SRP54
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:11301. SRP54.

Subcellular locationi

Nucleus speckle 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. nucleolus Source: HPA
  4. nucleus Source: UniProtKB
  5. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signal recognition particle

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36125.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Signal recognition particle 54 kDa proteinPRO_0000101192Add
BLAST

Proteomic databases

MaxQBiP61011.
PaxDbiP61011.
PRIDEiP61011.

PTM databases

PhosphoSiteiP61011.

Expressioni

Gene expression databases

BgeeiP61011.
CleanExiHS_SRP54.
ExpressionAtlasiP61011. baseline and differential.
GenevestigatoriP61011.

Organism-specific databases

HPAiHPA048977.

Interactioni

Subunit structurei

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1.2 Publications

Protein-protein interaction databases

BioGridi112607. 23 interactions.
IntActiP61011. 3 interactions.
MINTiMINT-3022178.
STRINGi9606.ENSP00000216774.

Structurei

Secondary structure

1
504
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi329 – 34113Combined sources
Helixi343 – 3519Combined sources
Turni357 – 3604Combined sources
Helixi365 – 37915Combined sources
Helixi384 – 3885Combined sources
Turni390 – 3923Combined sources
Helixi393 – 3986Combined sources
Helixi401 – 4099Combined sources
Helixi414 – 43017Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MFQX-ray3.10C323-441[»]
1QB2X-ray2.10A/B326-434[»]
ProteinModelPortaliP61011.
SMRiP61011. Positions 8-488.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61011.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 295295G-domainAdd
BLAST
Regioni296 – 504209M-domainAdd
BLAST

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0541.
GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
HOVERGENiHBG061072.
InParanoidiP61011.
KOiK03106.
OMAiEEMRMIS.
OrthoDBiEOG747PHN.
PhylomeDBiP61011.
TreeFamiTF106249.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P61011) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK
60 70 80 90 100
QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK
110 120 130 140 150
QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ
160 170 180 190 200
NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS
210 220 230 240 250
LFEEMLQVAN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL
260 270 280 290 300
DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI
310 320 330 340 350
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG
360 370 380 390 400
MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP
410 420 430 440 450
GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV
460 470 480 490 500
SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG

FNNM
Length:504
Mass (Da):55,705
Last modified:April 26, 2004 - v1
Checksum:i7D7630A43BC5EC2C
GO
Isoform 2 (identifier: P61011-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVK → MPPLSMKR

Note: No experimental confirmation available.

Show »
Length:455
Mass (Da):50,369
Checksum:i828728336FA34882
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5757MVLAD…RENVK → MPPLSMKR in isoform 2. 1 PublicationVSP_043696Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86373 Genomic DNA. Translation: CAA60132.1.
U51920 mRNA. Translation: AAC50994.1.
AK300824 mRNA. Translation: BAG62478.1.
AK312853 mRNA. Translation: BAG35706.1.
AL049776 Genomic DNA. No translation available.
BC000652 mRNA. Translation: AAH00652.1.
BC003389 mRNA. Translation: AAH03389.1.
CCDSiCCDS53893.1. [P61011-2]
CCDS9652.1. [P61011-1]
PIRiS54143.
RefSeqiNP_001139754.1. NM_001146282.1. [P61011-2]
NP_003127.1. NM_003136.3. [P61011-1]
UniGeneiHs.167535.

Genome annotation databases

EnsembliENST00000216774; ENSP00000216774; ENSG00000100883. [P61011-1]
ENST00000546080; ENSP00000440629; ENSG00000100883. [P61011-2]
ENST00000556994; ENSP00000451818; ENSG00000100883. [P61011-1]
GeneIDi6729.
KEGGihsa:6729.
UCSCiuc001wso.3. human. [P61011-1]
uc010tpp.2. human. [P61011-2]

Polymorphism databases

DMDMi46577650.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Wikipedia

Signal recognition particle entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X86373 Genomic DNA. Translation: CAA60132.1 .
U51920 mRNA. Translation: AAC50994.1 .
AK300824 mRNA. Translation: BAG62478.1 .
AK312853 mRNA. Translation: BAG35706.1 .
AL049776 Genomic DNA. No translation available.
BC000652 mRNA. Translation: AAH00652.1 .
BC003389 mRNA. Translation: AAH03389.1 .
CCDSi CCDS53893.1. [P61011-2 ]
CCDS9652.1. [P61011-1 ]
PIRi S54143.
RefSeqi NP_001139754.1. NM_001146282.1. [P61011-2 ]
NP_003127.1. NM_003136.3. [P61011-1 ]
UniGenei Hs.167535.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MFQ X-ray 3.10 C 323-441 [» ]
1QB2 X-ray 2.10 A/B 326-434 [» ]
ProteinModelPortali P61011.
SMRi P61011. Positions 8-488.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112607. 23 interactions.
IntActi P61011. 3 interactions.
MINTi MINT-3022178.
STRINGi 9606.ENSP00000216774.

Protein family/group databases

TCDBi 3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

PhosphoSitei P61011.

Polymorphism databases

DMDMi 46577650.

Proteomic databases

MaxQBi P61011.
PaxDbi P61011.
PRIDEi P61011.

Protocols and materials databases

DNASUi 6729.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000216774 ; ENSP00000216774 ; ENSG00000100883 . [P61011-1 ]
ENST00000546080 ; ENSP00000440629 ; ENSG00000100883 . [P61011-2 ]
ENST00000556994 ; ENSP00000451818 ; ENSG00000100883 . [P61011-1 ]
GeneIDi 6729.
KEGGi hsa:6729.
UCSCi uc001wso.3. human. [P61011-1 ]
uc010tpp.2. human. [P61011-2 ]

Organism-specific databases

CTDi 6729.
GeneCardsi GC14P035452.
HGNCi HGNC:11301. SRP54.
HPAi HPA048977.
MIMi 604857. gene.
neXtProti NX_P61011.
PharmGKBi PA36125.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0541.
GeneTreei ENSGT00550000074824.
HOGENOMi HOG000036165.
HOVERGENi HBG061072.
InParanoidi P61011.
KOi K03106.
OMAi EEMRMIS.
OrthoDBi EOG747PHN.
PhylomeDBi P61011.
TreeFami TF106249.

Enzyme and pathway databases

Reactomei REACT_115902. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

ChiTaRSi SRP54. human.
EvolutionaryTracei P61011.
GenomeRNAii 6729.
NextBioi 26248.
PROi P61011.
SOURCEi Search...

Gene expression databases

Bgeei P61011.
CleanExi HS_SRP54.
ExpressionAtlasi P61011. baseline and differential.
Genevestigatori P61011.

Family and domain databases

Gene3Di 1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00306. SRP54.
InterProi IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view ]
Pfami PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01425. SRP54_euk. 1 hit.
PROSITEi PS00300. SRP54. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the highly conserved gene encoding the human 54kDa subunit of signal recognition particle."
    Patel S., Austen B.
    DNA Seq. 6:167-170(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  2. "Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites."
    Gowda K., Black S.D., Moeller I., Sakakibara Y., Liu M.C., Zwieb C.
    Gene 207:197-207(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Trachea.
  4. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Placenta.
  6. "Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo."
    Sakashita E., Tatsumi S., Werner D., Endo H., Mayeda A.
    Mol. Cell. Biol. 24:1174-1187(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNPS1, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of the conserved subdomain of human protein SRP54M at 2.1 A resolution: evidence for the mechanism of signal peptide binding."
    Clemons W.M. Jr., Gowda K., Black S.D., Zwieb C., Ramakrishnan V.
    J. Mol. Biol. 292:697-705(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 326-432.
  10. "Induced structural changes of 7SL RNA during the assembly of human signal recognition particle."
    Kuglstatter A., Oubridge C., Nagai K.
    Nat. Struct. Biol. 9:740-744(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 323-441 IN COMPLEX WITH SRP19 AND 7SL RNA.

Entry informationi

Entry nameiSRP54_HUMAN
AccessioniPrimary (citable) accession number: P61011
Secondary accession number(s): B2R759, B4DUW6, P13624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3