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Protein

Signal recognition particle 54 kDa protein

Gene

SRP54

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115GTPBy similarity8
Nucleotide bindingi190 – 194GTPBy similarity5
Nucleotide bindingi248 – 251GTPBy similarity4

GO - Molecular functioni

  • 7S RNA binding Source: UniProtKB
  • drug binding Source: UniProtKB
  • endoplasmic reticulum signal peptide binding Source: UniProtKB
  • GDP binding Source: UniProtKB
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • ribonucleoprotein complex binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100883-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.

Protein family/group databases

TCDBi3.A.5.9.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein
Short name:
SRP54
Gene namesi
Name:SRP54
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:11301. SRP54.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • nuclear speck Source: UniProtKB-SubCell
  • nucleolus Source: HPA
  • nucleus Source: UniProtKB
  • signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signal recognition particle

Pathology & Biotechi

Organism-specific databases

DisGeNETi6729.
OpenTargetsiENSG00000100883.
PharmGKBiPA36125.

Polymorphism and mutation databases

BioMutaiSRP54.
DMDMi46577650.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011921 – 504Signal recognition particle 54 kDa proteinAdd BLAST504

Proteomic databases

EPDiP61011.
MaxQBiP61011.
PaxDbiP61011.
PeptideAtlasiP61011.
PRIDEiP61011.

PTM databases

iPTMnetiP61011.
PhosphoSitePlusiP61011.
SwissPalmiP61011.

Expressioni

Gene expression databases

BgeeiENSG00000100883.
CleanExiHS_SRP54.
ExpressionAtlasiP61011. baseline and differential.
GenevisibleiP61011. HS.

Organism-specific databases

HPAiHPA048977.
HPA062044.

Interactioni

Subunit structurei

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1.2 Publications

Protein-protein interaction databases

BioGridi112607. 34 interactors.
IntActiP61011. 9 interactors.
MINTiMINT-3022178.
STRINGi9606.ENSP00000216774.

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 41Combined sources17
Helixi46 – 58Combined sources13
Helixi71 – 74Combined sources4
Helixi76 – 87Combined sources12
Beta strandi102 – 107Combined sources6
Helixi114 – 127Combined sources14
Beta strandi132 – 137Combined sources6
Helixi144 – 154Combined sources11
Helixi168 – 180Combined sources13
Turni181 – 183Combined sources3
Beta strandi185 – 191Combined sources7
Helixi199 – 212Combined sources14
Beta strandi215 – 222Combined sources8
Helixi229 – 239Combined sources11
Beta strandi242 – 248Combined sources7
Helixi258 – 266Combined sources9
Beta strandi270 – 274Combined sources5
Beta strandi276 – 278Combined sources3
Beta strandi282 – 284Combined sources3
Helixi287 – 295Combined sources9
Helixi329 – 341Combined sources13
Helixi343 – 351Combined sources9
Turni357 – 360Combined sources4
Helixi365 – 379Combined sources15
Helixi384 – 388Combined sources5
Turni390 – 392Combined sources3
Helixi393 – 398Combined sources6
Helixi401 – 409Combined sources9
Helixi414 – 430Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MFQX-ray3.10C323-441[»]
1QB2X-ray2.10A/B326-434[»]
5L3QX-ray3.20A/C1-436[»]
ProteinModelPortaliP61011.
SMRiP61011.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61011.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 295G-domainAdd BLAST295
Regioni296 – 504M-domainAdd BLAST209

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0780. Eukaryota.
COG0541. LUCA.
GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
HOVERGENiHBG061072.
InParanoidiP61011.
KOiK03106.
OMAiKMGPMKQ.
OrthoDBiEOG091G05OW.
PhylomeDBiP61011.
TreeFamiTF106249.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61011-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK
60 70 80 90 100
QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK
110 120 130 140 150
QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ
160 170 180 190 200
NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS
210 220 230 240 250
LFEEMLQVAN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL
260 270 280 290 300
DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI
310 320 330 340 350
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG
360 370 380 390 400
MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP
410 420 430 440 450
GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV
460 470 480 490 500
SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG

FNNM
Length:504
Mass (Da):55,705
Last modified:April 26, 2004 - v1
Checksum:i7D7630A43BC5EC2C
GO
Isoform 2 (identifier: P61011-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVK → MPPLSMKR

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):50,369
Checksum:i828728336FA34882
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0436961 – 57MVLAD…RENVK → MPPLSMKR in isoform 2. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86373 Genomic DNA. Translation: CAA60132.1.
U51920 mRNA. Translation: AAC50994.1.
AK300824 mRNA. Translation: BAG62478.1.
AK312853 mRNA. Translation: BAG35706.1.
AL049776 Genomic DNA. No translation available.
BC000652 mRNA. Translation: AAH00652.1.
BC003389 mRNA. Translation: AAH03389.1.
CCDSiCCDS53893.1. [P61011-2]
CCDS9652.1. [P61011-1]
PIRiS54143.
RefSeqiNP_001139754.1. NM_001146282.1. [P61011-2]
NP_003127.1. NM_003136.3. [P61011-1]
XP_011535408.1. XM_011537106.1. [P61011-1]
UniGeneiHs.167535.

Genome annotation databases

EnsembliENST00000216774; ENSP00000216774; ENSG00000100883. [P61011-1]
ENST00000546080; ENSP00000440629; ENSG00000100883. [P61011-2]
ENST00000556994; ENSP00000451818; ENSG00000100883. [P61011-1]
GeneIDi6729.
KEGGihsa:6729.
UCSCiuc001wso.4. human. [P61011-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Wikipedia

Signal recognition particle entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86373 Genomic DNA. Translation: CAA60132.1.
U51920 mRNA. Translation: AAC50994.1.
AK300824 mRNA. Translation: BAG62478.1.
AK312853 mRNA. Translation: BAG35706.1.
AL049776 Genomic DNA. No translation available.
BC000652 mRNA. Translation: AAH00652.1.
BC003389 mRNA. Translation: AAH03389.1.
CCDSiCCDS53893.1. [P61011-2]
CCDS9652.1. [P61011-1]
PIRiS54143.
RefSeqiNP_001139754.1. NM_001146282.1. [P61011-2]
NP_003127.1. NM_003136.3. [P61011-1]
XP_011535408.1. XM_011537106.1. [P61011-1]
UniGeneiHs.167535.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MFQX-ray3.10C323-441[»]
1QB2X-ray2.10A/B326-434[»]
5L3QX-ray3.20A/C1-436[»]
ProteinModelPortaliP61011.
SMRiP61011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112607. 34 interactors.
IntActiP61011. 9 interactors.
MINTiMINT-3022178.
STRINGi9606.ENSP00000216774.

Protein family/group databases

TCDBi3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

iPTMnetiP61011.
PhosphoSitePlusiP61011.
SwissPalmiP61011.

Polymorphism and mutation databases

BioMutaiSRP54.
DMDMi46577650.

Proteomic databases

EPDiP61011.
MaxQBiP61011.
PaxDbiP61011.
PeptideAtlasiP61011.
PRIDEiP61011.

Protocols and materials databases

DNASUi6729.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216774; ENSP00000216774; ENSG00000100883. [P61011-1]
ENST00000546080; ENSP00000440629; ENSG00000100883. [P61011-2]
ENST00000556994; ENSP00000451818; ENSG00000100883. [P61011-1]
GeneIDi6729.
KEGGihsa:6729.
UCSCiuc001wso.4. human. [P61011-1]

Organism-specific databases

CTDi6729.
DisGeNETi6729.
GeneCardsiSRP54.
HGNCiHGNC:11301. SRP54.
HPAiHPA048977.
HPA062044.
MIMi604857. gene.
neXtProtiNX_P61011.
OpenTargetsiENSG00000100883.
PharmGKBiPA36125.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0780. Eukaryota.
COG0541. LUCA.
GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
HOVERGENiHBG061072.
InParanoidiP61011.
KOiK03106.
OMAiKMGPMKQ.
OrthoDBiEOG091G05OW.
PhylomeDBiP61011.
TreeFamiTF106249.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100883-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

ChiTaRSiSRP54. human.
EvolutionaryTraceiP61011.
GenomeRNAii6729.
PROiP61011.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100883.
CleanExiHS_SRP54.
ExpressionAtlasiP61011. baseline and differential.
GenevisibleiP61011. HS.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRP54_HUMAN
AccessioniPrimary (citable) accession number: P61011
Secondary accession number(s): B2R759, B4DUW6, P13624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.