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P61011 (SRP54_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal recognition particle 54 kDa protein

Short name=SRP54
Gene names
Name:SRP54
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). HAMAP-Rule MF_00306

Subunit structure

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1. Ref.6

Subcellular location

Nucleus speckle. Cytoplasm Ref.6.

Domain

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
Signal recognition particle
   Coding sequence diversityAlternative splicing
   LigandGTP-binding
Nucleotide-binding
RNA-binding
   Molecular functionRibonucleoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay PubMed 8247130. Source: GOC

SRP-dependent cotranslational protein targeting to membrane

Traceable author statement. Source: Reactome

SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition

Inferred from sequence or structural similarity. Source: UniProtKB

SRP-dependent cotranslational protein targeting to membrane, translocation

Inferred from sequence or structural similarity. Source: UniProtKB

cellular protein metabolic process

Traceable author statement. Source: Reactome

gene expression

Traceable author statement. Source: Reactome

protein targeting to ER

Inferred from mutant phenotype PubMed 18089836. Source: UniProtKB

response to drug

Inferred from direct assay PubMed 18089836. Source: UniProtKB

translation

Traceable author statement. Source: Reactome

   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

cytosol

Traceable author statement. Source: Reactome

nuclear speck

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleolus

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay PubMed 18089836. Source: UniProtKB

signal recognition particle, endoplasmic reticulum targeting

Inferred from direct assay PubMed 18089836. Source: UniProtKB

   Molecular_function7S RNA binding

Inferred from direct assay PubMed 18089836Ref.2. Source: UniProtKB

GDP binding

Inferred from direct assay PubMed 8622769. Source: UniProtKB

GTP binding

Inferred from direct assay PubMed 8622769. Source: UniProtKB

GTPase activity

Inferred from electronic annotation. Source: InterPro

drug binding

Inferred from direct assay PubMed 18089836. Source: UniProtKB

endoplasmic reticulum signal peptide binding

Inferred from direct assay Ref.2. Source: UniProtKB

poly(A) RNA binding

Inferred from direct assay PubMed 22658674. Source: UniProtKB

ribonucleoprotein complex binding

Inferred from direct assay Ref.2. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P61011-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P61011-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVK → MPPLSMKR
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Signal recognition particle 54 kDa protein HAMAP-Rule MF_00306
PRO_0000101192

Regions

Nucleotide binding108 – 1158GTP By similarity
Nucleotide binding190 – 1945GTP By similarity
Nucleotide binding248 – 2514GTP By similarity
Region1 – 295295G-domain HAMAP-Rule MF_00306
Region296 – 504209M-domain HAMAP-Rule MF_00306

Natural variations

Alternative sequence1 – 5757MVLAD…RENVK → MPPLSMKR in isoform 2.
VSP_043696

Secondary structure

.................. 504
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 26, 2004. Version 1.
Checksum: 7D7630A43BC5EC2C

FASTA50455,705
        10         20         30         40         50         60 
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI 

        70         80         90        100        110        120 
DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK QNVIMFVGLQ GSGKTTTCSK 

       130        140        150        160        170        180 
LAYYYQRKGW KTCLICADTF RAGAFDQLKQ NATKARIPFY GSYTEMDPVI IASEGVEKFK 

       190        200        210        220        230        240 
NENFEIIIVD TSGRHKQEDS LFEEMLQVAN AIQPDNIVYV MDASIGQACE AQAKAFKDKV 

       250        260        270        280        290        300 
DVASVIVTKL DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI 

       310        320        330        340        350        360 
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG MIPGFGTDFM 

       370        380        390        400        410        420 
SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP GRIQRVARGS GVSTRDVQEL 

       430        440        450        460        470        480 
LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL 

       490        500 
QSMMRQFQQG AAGNMKGMMG FNNM 

« Hide

Isoform 2 [UniParc].

Checksum: 828728336FA34882
Show »

FASTA45550,369

References

« Hide 'large scale' references
[1]"Sequence of the highly conserved gene encoding the human 54kDa subunit of signal recognition particle."
Patel S., Austen B.
DNA Seq. 6:167-170(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[2]"Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites."
Gowda K., Black S.D., Moeller I., Sakakibara Y., Liu M.C., Zwieb C.
Gene 207:197-207(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Trachea.
[4]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye and Placenta.
[6]"Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo."
Sakashita E., Tatsumi S., Werner D., Endo H., Mayeda A.
Mol. Cell. Biol. 24:1174-1187(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNPS1, SUBCELLULAR LOCATION.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Crystal structure of the conserved subdomain of human protein SRP54M at 2.1 A resolution: evidence for the mechanism of signal peptide binding."
Clemons W.M. Jr., Gowda K., Black S.D., Zwieb C., Ramakrishnan V.
J. Mol. Biol. 292:697-705(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 326-432.
[10]"Induced structural changes of 7SL RNA during the assembly of human signal recognition particle."
Kuglstatter A., Oubridge C., Nagai K.
Nat. Struct. Biol. 9:740-744(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 323-441 IN COMPLEX WITH SRP19 AND 7SL RNA.
+Additional computationally mapped references.

Web resources

Wikipedia

Signal recognition particle entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X86373 Genomic DNA. Translation: CAA60132.1.
U51920 mRNA. Translation: AAC50994.1.
AK300824 mRNA. Translation: BAG62478.1.
AK312853 mRNA. Translation: BAG35706.1.
AL049776 Genomic DNA. No translation available.
BC000652 mRNA. Translation: AAH00652.1.
BC003389 mRNA. Translation: AAH03389.1.
CCDSCCDS53893.1. [P61011-2]
CCDS9652.1. [P61011-1]
PIRS54143.
RefSeqNP_001139754.1. NM_001146282.1. [P61011-2]
NP_003127.1. NM_003136.3. [P61011-1]
UniGeneHs.167535.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MFQX-ray3.10C323-441[»]
1QB2X-ray2.10A/B326-434[»]
ProteinModelPortalP61011.
SMRP61011. Positions 8-488.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112607. 22 interactions.
IntActP61011. 3 interactions.
MINTMINT-3022178.
STRING9606.ENSP00000216774.

Protein family/group databases

TCDB3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

PhosphoSiteP61011.

Polymorphism databases

DMDM46577650.

Proteomic databases

MaxQBP61011.
PaxDbP61011.
PRIDEP61011.

Protocols and materials databases

DNASU6729.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000216774; ENSP00000216774; ENSG00000100883. [P61011-1]
ENST00000546080; ENSP00000440629; ENSG00000100883. [P61011-2]
ENST00000556994; ENSP00000451818; ENSG00000100883. [P61011-1]
GeneID6729.
KEGGhsa:6729.
UCSCuc001wso.3. human. [P61011-1]
uc010tpp.2. human. [P61011-2]

Organism-specific databases

CTD6729.
GeneCardsGC14P035452.
HGNCHGNC:11301. SRP54.
HPAHPA048977.
MIM604857. gene.
neXtProtNX_P61011.
PharmGKBPA36125.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0541.
HOGENOMHOG000036165.
HOVERGENHBG061072.
InParanoidP61011.
KOK03106.
OMAEEMRMIS.
OrthoDBEOG747PHN.
PhylomeDBP61011.
TreeFamTF106249.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressP61011.
BgeeP61011.
CleanExHS_SRP54.
GenevestigatorP61011.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01425. SRP54_euk. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSRP54. human.
EvolutionaryTraceP61011.
GenomeRNAi6729.
NextBio26248.
PROP61011.
SOURCESearch...

Entry information

Entry nameSRP54_HUMAN
AccessionPrimary (citable) accession number: P61011
Secondary accession number(s): B2R759, B4DUW6, P13624
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM