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Protein

Signal recognition particle 54 kDa protein

Gene

SRP54

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115GTPBy similarity8
Nucleotide bindingi190 – 194GTPBy similarity5
Nucleotide bindingi248 – 251GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • protein targeting to ER Source: UniProtKB
  • response to drug Source: UniProtKB
  • SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  • SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Source: UniProtKB
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-CFA-1799339. SRP-dependent cotranslational protein targeting to membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein
Short name:
SRP54
Gene namesi
Name:SRP54
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011911 – 504Signal recognition particle 54 kDa proteinAdd BLAST504

Proteomic databases

PaxDbiP61010.
PRIDEiP61010.

Interactioni

Subunit structurei

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000019649.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GO5electron microscopy7.40W326-434[»]
2J37electron microscopy8.00W1-504[»]
4UE5electron microscopy9.00D1-433[»]
ProteinModelPortaliP61010.
SMRiP61010.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61010.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 295G-domainAdd BLAST295
Regioni296 – 504M-domainAdd BLAST209

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0780. Eukaryota.
COG0541. LUCA.
GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
HOVERGENiHBG061072.
InParanoidiP61010.
KOiK03106.
OMAiKMGPMKQ.
OrthoDBiEOG091G05OW.
TreeFamiTF106249.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK
60 70 80 90 100
QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK
110 120 130 140 150
QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ
160 170 180 190 200
NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS
210 220 230 240 250
LFEEMLQVAN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL
260 270 280 290 300
DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI
310 320 330 340 350
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG
360 370 380 390 400
MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP
410 420 430 440 450
GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV
460 470 480 490 500
SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG

FNNM
Length:504
Mass (Da):55,705
Last modified:April 26, 2004 - v1
Checksum:i7D7630A43BC5EC2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16318 mRNA. Translation: CAA34385.1.
PIRiS05197.
RefSeqiNP_001003272.1. NM_001003272.1.
XP_005623237.1. XM_005623180.2.
XP_005623238.1. XM_005623181.2.
UniGeneiCfa.3810.

Genome annotation databases

EnsembliENSCAFT00000021158; ENSCAFP00000019649; ENSCAFG00000013331.
GeneIDi403953.
KEGGicfa:403953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16318 mRNA. Translation: CAA34385.1.
PIRiS05197.
RefSeqiNP_001003272.1. NM_001003272.1.
XP_005623237.1. XM_005623180.2.
XP_005623238.1. XM_005623181.2.
UniGeneiCfa.3810.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GO5electron microscopy7.40W326-434[»]
2J37electron microscopy8.00W1-504[»]
4UE5electron microscopy9.00D1-433[»]
ProteinModelPortaliP61010.
SMRiP61010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000019649.

Proteomic databases

PaxDbiP61010.
PRIDEiP61010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000021158; ENSCAFP00000019649; ENSCAFG00000013331.
GeneIDi403953.
KEGGicfa:403953.

Organism-specific databases

CTDi6729.

Phylogenomic databases

eggNOGiKOG0780. Eukaryota.
COG0541. LUCA.
GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
HOVERGENiHBG061072.
InParanoidiP61010.
KOiK03106.
OMAiKMGPMKQ.
OrthoDBiEOG091G05OW.
TreeFamiTF106249.

Enzyme and pathway databases

ReactomeiR-CFA-1799339. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

EvolutionaryTraceiP61010.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRP54_CANLF
AccessioniPrimary (citable) accession number: P61010
Secondary accession number(s): P13624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.