P60994 (ERVB_TABDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ervatamin-B Short name=ERV-B EC=3.4.22.-
Organism	Tabernaemontana divaricata (Crepe jasmine) (Ervatamia coronaria)
Taxonomic identifier	52861 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Tabermontantaneae › Tabernaemontana

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cysteine protease.
Subcellular location	Secreted.
Tissue specificity	Laticifer.
Post-translational modification	Not glycosylated.
Miscellaneous	Stable in 8 M urea and 2.5 M GuHCl at neutral pH, in 40% acetonitrile, 70% ethanol and 50% methanol. Unstable in SDS.
Sequence similarities	Belongs to the peptidase C1 family.
Biophysicochemical properties	pH dependence: Active from pH 3.0 to 10.5. Temperature dependence: Active up to 62 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 215

215

Ervatamin-B

PRO_0000050558

Sites

Active site

25

1

Active site

158

1

Active site

178

1

By similarity

Amino acid modifications

Disulfide bond

22 ↔ 63

Disulfide bond

56 ↔ 96

Disulfide bond

152 ↔ 203

Secondary structure

1

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215

Helix Strand Turn

Details...

Helix

7 – 10

4

Beta strand

21 – 23

3

Helix

25 – 42

18

Helix

50 – 56

7

Helix

68 – 78

11

Turn

84 – 86

3

Beta strand

108 – 111

4

Helix

117 – 126

10

Beta strand

129 – 133

5

Helix

138 – 141

4

Beta strand

145 – 148

4

Beta strand

158 – 168

11

Beta strand

171 – 177

7

Beta strand

189 – 193

5

Helix

202 – 204

3

Beta strand

210 – 213

4

Sequences

	Sequence		Length	Mass (Da)
	P60994 [UniParc]. Last modified April 26, 2004. Version 1. Checksum: 4DE62E43BA4F4F83	FASTA	215	23,183
	10 20 30 40 50 60 LPSFVDWRSK GAVNSIKNQK QCGSCWAFSA VAAVESINKI RTGQLISLSE QELVDCDTAS 70 80 90 100 110 120 HGCNGGWMNN AFQYIITNGG IDTQQNYPYS AVQGSCKPYR LRVVSINGFQ RVTRNNESAL 130 140 150 160 170 180 QSAVASQPVS VTVEAAGAPF QHYSSGIFTG PCGTAQNHGV VIVGYGTQSG KNYWIVRNSW 190 200 210 GQNWGNQGYI WMERNVASSA GLCGIAQLPS YPTKA

References

[1]	"Proposed amino acid sequence and the 1.63 A X-ray crystal structure of a plant cysteine protease, ervatamin B: some insights into the structural basis of its stability and substrate specificity." Biswas S., Chakrabarti C., Kundu S., Jagannadham M.V., Dattagupta J.K. Proteins 51:489-497(2003) [PubMed: 12784208] [Abstract] Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[2]	"Purification and characterization of a stable cysteine protease ervatamin B, with two disulfide bridges, from the latex of Ervatamia coronaria." Kundu S., Sundd M., Jagannadham M.V. J. Agric. Food Chem. 48:171-179(2000) [PubMed: 10691612] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION. Tissue: Latex.

Cross-references

Sequence databases

PIR

A59428.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IWD	X-ray	1.63	A	1-215	[»]

ProteinModelPortal

P60994.

SMR

P60994. Positions 1-215.

ModBase

Search...

Protein family/group databases

MEROPS

C01.099.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000169. Pept_cys_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
[Graphical view]

PANTHER

PTHR12411. Peptidase_C1A. 1 hit.

Pfam

PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

SMART

SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ERVB_TABDI

Accession

Primary (citable) accession number: P60994

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program