Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P60992

- TX1_HETMC

UniProt

P60992 - TX1_HETMC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Kappa-theraphotoxin-Hm1a

Gene
N/A
Organism
Heteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Inhibitor of voltage-gated potassium channels. It inhibits Kv2.1/KCNB1, Kv2.2/KCNB2, Kv4.1/KCND1, Kv4.2/KCND2 and Kv4.3/KCND3 potassium channels. May act by shifting activation of the channel to more depolarized voltages. The toxin binding sites may be situated on the S3-S4 extracellular linker of the channel. One, two, three or four toxin molecules may bind the Kv2.1/KCNB1 channel. May need to partition into the membrane in order to bind to the channel. Intracerebroventricular injection into mice induces convulsions, spasms, tremors, and death.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei3 – 31May be involved in interaction with voltage sensorBy similarity
Sitei22 – 221May be involved in interaction with voltage sensorBy similarity
Sitei30 – 301Involved in active faceBy similarity

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

TCDBi8.B.5.3.3. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Hm1a
Short name:
Kappa-TRTX-Hm1a
Alternative name(s):
Heteroscodratoxin-1
HmTx1
OrganismiHeteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)
Taxonomic identifieri268413 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHeteroscodra

Organism-specific databases

ArachnoServeriAS000224. kappa-theraphotoxin-Hm1a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3535Kappa-theraphotoxin-Hm1aPRO_0000045017Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 16By similarity
Disulfide bondi9 ↔ 21By similarity
Disulfide bondi15 ↔ 28By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliP60992.
SMRiP60992. Positions 1-35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 63Involved in active faceBy similarity

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Belongs to the huwentoxin-1 family.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR011696. Ion_channel_inhibitory.
[Graphical view]
PfamiPF07740. Toxin_12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60992-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30 
ECRYLFGGCS STSDCCKHLS CRSDWKYCAW DGTFS
Length:35
Mass (Da):4,003
Last modified:April 26, 2004 - v1
Checksum:iB9A84D9FF50AE603
GO

Mass spectrometryi

Molecular mass is 3994.59 Da from positions 1 - 35. Determined by MALDI. 1 Publication

Cross-referencesi

3D structure databases

ProteinModelPortali P60992.
SMRi P60992. Positions 1-35.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 8.B.5.3.3. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ArachnoServeri AS000224. kappa-theraphotoxin-Hm1a.

Family and domain databases

InterProi IPR011696. Ion_channel_inhibitory.
[Graphical view ]
Pfami PF07740. Toxin_12. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel tarantula toxins for subtypes of voltage-dependent potassium channels in the Kv2 and Kv4 subfamilies."
    Escoubas P., Diochot S., Celerier M.-L., Nakajima T., Lazdunski M.
    Mol. Pharmacol. 62:48-57(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, 3D-STRUCTURE MODELING.
    Tissue: Venom.

Entry informationi

Entry nameiTX1_HETMC
AccessioniPrimary (citable) accession number: P60992
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

IC50 is 280 nM on Kv4.1/KCND1, whereas percentage of current inhibition are 23% on Kv2.1/KCNB1 at 100 nM, 19.7% on Kv2.2/KCNB2 at 100 nM, 39% on Kv4.2/KCND2 at 300 nM, and 43% on Kv4.3/KCND3 at 300 nM.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3