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Protein

Kappa-theraphotoxin-Gr3a

Gene
N/A
Organism
Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits sodium channels Nav1.7/SCN9A and potassium channels Kv11.1/KCNH2. Also binds the voltage-sensor domain of the potassium channel KvAP (from the archaeon Aeropyrum pernix) with very slow apparent binding kinetics and affects channel gating. Reaches its target by dynamically partitioning into anionic or zwitterionic headgroup lipid membranes. May bind to the open state of KvAP.4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBi8.B.5.3.8. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-theraphotoxin-Gr3a
Short name:
Kappa-TRTX-Gr3a
Alternative name(s):
Voltage sensor toxin 1
Short name:
VsTx1
OrganismiGrammostola rosea (Chilean rose tarantula) (Grammostola spatulata)
Taxonomic identifieri432528 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeGrammostola

Organism-specific databases

ArachnoServeriAS000423. kappa-theraphotoxin-Gr3a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000043482122 – 29Curated8
PeptideiPRO_000004500830 – 63Kappa-theraphotoxin-Gr3aAdd BLAST34

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 451 Publication
Disulfide bondi38 ↔ 501 Publication
Disulfide bondi44 ↔ 571 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

163
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 41Combined sources3
Beta strandi48 – 51Combined sources4
Turni52 – 55Combined sources4
Beta strandi56 – 59Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S6XNMR-A30-63[»]
2N1NNMR-A30-63[»]
ProteinModelPortaliP60980.
SMRiP60980.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60980.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. VsTx1 subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
[Graphical view]
PfamiPF07740. Toxin_12. 1 hit.
[Graphical view]
PROSITEiPS60021. HWTX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTSVFVLVL GLVLLFAVSF ATEMEESARE CGKFMWKCKN SNDCCKDLVC
60
SSRWKWCVLA SPF
Length:63
Mass (Da):7,175
Last modified:December 9, 2015 - v2
Checksum:i931A386CF4122268
GO

Mass spectrometryi

Molecular mass is 3997.0 Da from positions 30 - 63. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB200994 mRNA. Translation: BAN13490.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB200994 mRNA. Translation: BAN13490.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S6XNMR-A30-63[»]
2N1NNMR-A30-63[»]
ProteinModelPortaliP60980.
SMRiP60980.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.5.3.8. the na(+)/k(+)/ca(2+) channel targeting tarantula huwentoxin (tht) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000423. kappa-theraphotoxin-Gr3a.

Miscellaneous databases

EvolutionaryTraceiP60980.

Family and domain databases

InterProiIPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
[Graphical view]
PfamiPF07740. Toxin_12. 1 hit.
[Graphical view]
PROSITEiPS60021. HWTX_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVSTX1_GRARO
AccessioniPrimary (citable) accession number: P60980
Secondary accession number(s): M5AY63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: December 9, 2015
Last modified: November 2, 2016
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not inhibit potassium channels Kv1.1/KCNA1 (IC50>200 µM), Kv1.4/KCNA4 (IC50>200 µM), Kv11.2/KCNH6 (IC50=45 µM) and Kv11.3/KCNH7 (IC50=55 µM) and sodium channels Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A, Nav1.6/SCN8A.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.