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Protein

Prolipoprotein diacylglyceryl transferase

Gene

lgt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins.1 Publication

Pathwayi: lipoprotein biosynthesis (diacylglyceryl transfer)

This protein is involved in the pathway lipoprotein biosynthesis (diacylglyceryl transfer), which is part of Protein modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (diacylglyceryl transfer) and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • lipoprotein biosynthetic process Source: EcoCyc
  • protein lipoylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG12128-MONOMER.
ECOL316407:JW2796-MONOMER.
MetaCyc:EG12128-MONOMER.
UniPathwayiUPA00664.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolipoprotein diacylglyceryl transferase (EC:2.4.99.-)
Gene namesi
Name:lgt
Synonyms:umpA
Ordered Locus Names:b2828Imported, JW2796Imported
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12128. lgt.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20Periplasmic1 PublicationAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 59Cytoplasmic1 PublicationAdd BLAST18
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 95Periplasmic1 PublicationAdd BLAST15
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 129Cytoplasmic1 PublicationAdd BLAST13
Transmembranei130 – 150HelicalSequence analysisAdd BLAST21
Topological domaini151 – 197Periplasmic1 PublicationAdd BLAST47
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 224Cytoplasmic1 Publication6
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Topological domaini246 – 259Periplasmic1 PublicationAdd BLAST14
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Topological domaini281 – 291Cytoplasmic2 PublicationsAdd BLAST11

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants are defective in diacylglyceryl modification of prolipoprotein.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7H → Q: No effect. 1 Publication1
Mutagenesisi24H → Q: No effect. 1 Publication1
Mutagenesisi26Y → A: Loss of activity. 1 Publication1
Mutagenesisi26Y → F: No effect. 1 Publication1
Mutagenesisi62Y → F: No effect. 1 Publication1
Mutagenesisi76Y → F: No effect. 1 Publication1
Mutagenesisi98G → A: No effect. 1 Publication1
Mutagenesisi103H → N: Loss of activity. 1 Publication1
Mutagenesisi103H → Q: Loss of activity (PubMed:9139912). No effect (PubMed:22287519). 2 Publications1
Mutagenesisi104G → A: No effect. 1 Publication1
Mutagenesisi129D → A: No effect. 1 Publication1
Mutagenesisi143R → A: Decrease in activity. 1 Publication1
Mutagenesisi146N → A: Loss of activity. 1 Publication1
Mutagenesisi151E → A: Decrease in activity. 1 Publication1
Mutagenesisi154G → A: Loss of activity. 1 Publication1
Mutagenesisi190Y → F: No effect. 1 Publication1
Mutagenesisi196H → N or L: No effect. 1 Publication1
Mutagenesisi196H → Q or R: 50% decrease in activity. 1 Publication1
Mutagenesisi201Y → F: No effect. 1 Publication1
Mutagenesisi235Y → F or T: Loss of activity. 1 Publication1
Mutagenesisi235Y → S: 37% decrease in activity. 1 Publication1
Mutagenesisi239R → A: Decrease in activity. 1 Publication1
Mutagenesisi243E → A: Decrease in activity. 1 Publication1
Mutagenesisi282Y → F: No effect. 1 Publication1
Mutagenesisi289H → Q: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001725961 – 291Prolipoprotein diacylglyceryl transferaseAdd BLAST291

Proteomic databases

PaxDbiP60955.
PRIDEiP60955.

Interactioni

Protein-protein interaction databases

BioGridi4263276. 97 interactors.
STRINGi511145.b2828.

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Beta strandi13 – 18Combined sources6
Beta strandi21 – 24Combined sources4
Helixi25 – 45Combined sources21
Helixi54 – 80Combined sources27
Helixi82 – 87Combined sources6
Helixi89 – 93Combined sources5
Helixi95 – 97Combined sources3
Helixi102 – 119Combined sources18
Helixi124 – 131Combined sources8
Helixi132 – 134Combined sources3
Helixi135 – 148Combined sources14
Helixi167 – 169Combined sources3
Helixi170 – 177Combined sources8
Helixi181 – 183Combined sources3
Helixi184 – 190Combined sources7
Helixi198 – 206Combined sources9
Helixi208 – 217Combined sources10
Helixi226 – 243Combined sources18
Helixi255 – 284Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AZBX-ray1.60A2-291[»]
5AZCX-ray1.90A2-291[»]
ProteinModelPortaliP60955.
SMRiP60955.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Lgt family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3B. Bacteria.
COG0682. LUCA.
HOGENOMiHOG000098666.
InParanoidiP60955.
KOiK13292.
OMAiAHRTKRH.
PhylomeDBiP60955.

Family and domain databases

HAMAPiMF_01147. Lgt. 1 hit.
InterProiIPR001640. Prolipoprot_diAcglycer_Trfase.
[Graphical view]
PfamiPF01790. LGT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00544. lgt. 1 hit.
PROSITEiPS01311. LGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSYLHFPE FDPVIFSIGP VALHWYGLMY LVGFIFAMWL ATRRANRPGS
60 70 80 90 100
GWTKNEVENL LYAGFLGVFL GGRIGYVLFY NFPQFMADPL YLFRVWDGGM
110 120 130 140 150
SFHGGLIGVI VVMIIFARRT KRSFFQVSDF IAPLIPFGLG AGRLGNFING
160 170 180 190 200
ELWGRVDPNF PFAMLFPGSR TEDILLLQTN PQWQSIFDTY GVLPRHPSQL
210 220 230 240 250
YELLLEGVVL FIILNLYIRK PRPMGAVSGL FLIGYGAFRI IVEFFRQPDA
260 270 280 290
QFTGAWVQYI SMGQILSIPM IVAGVIMMVW AYRRSPQQHV S
Length:291
Mass (Da):33,108
Last modified:April 26, 2004 - v1
Checksum:i5F946E4E60D0112A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti25W → R in SK636, temperature-sensitive. 1
Natural varianti104G → S in SK634, temperature-sensitive. 1
Natural varianti139L → F in SK635, temperature-sensitive. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12289 Genomic DNA. Translation: AAA69024.1.
U29581 Genomic DNA. Translation: AAB40475.1.
U00096 Genomic DNA. Translation: AAC75867.1.
AP009048 Genomic DNA. Translation: BAE76897.1.
J01710 Genomic DNA. No translation available.
PIRiA56149.
RefSeqiNP_417305.1. NC_000913.3.
WP_000204658.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75867; AAC75867; b2828.
BAE76897; BAE76897; BAE76897.
GeneIDi947303.
KEGGiecj:JW2796.
eco:b2828.
PATRICi32121074. VBIEscCol129921_2926.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12289 Genomic DNA. Translation: AAA69024.1.
U29581 Genomic DNA. Translation: AAB40475.1.
U00096 Genomic DNA. Translation: AAC75867.1.
AP009048 Genomic DNA. Translation: BAE76897.1.
J01710 Genomic DNA. No translation available.
PIRiA56149.
RefSeqiNP_417305.1. NC_000913.3.
WP_000204658.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AZBX-ray1.60A2-291[»]
5AZCX-ray1.90A2-291[»]
ProteinModelPortaliP60955.
SMRiP60955.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263276. 97 interactors.
STRINGi511145.b2828.

Proteomic databases

PaxDbiP60955.
PRIDEiP60955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75867; AAC75867; b2828.
BAE76897; BAE76897; BAE76897.
GeneIDi947303.
KEGGiecj:JW2796.
eco:b2828.
PATRICi32121074. VBIEscCol129921_2926.

Organism-specific databases

EchoBASEiEB2049.
EcoGeneiEG12128. lgt.

Phylogenomic databases

eggNOGiENOG4105C3B. Bacteria.
COG0682. LUCA.
HOGENOMiHOG000098666.
InParanoidiP60955.
KOiK13292.
OMAiAHRTKRH.
PhylomeDBiP60955.

Enzyme and pathway databases

UniPathwayiUPA00664.
BioCyciEcoCyc:EG12128-MONOMER.
ECOL316407:JW2796-MONOMER.
MetaCyc:EG12128-MONOMER.

Miscellaneous databases

PROiP60955.

Family and domain databases

HAMAPiMF_01147. Lgt. 1 hit.
InterProiIPR001640. Prolipoprot_diAcglycer_Trfase.
[Graphical view]
PfamiPF01790. LGT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00544. lgt. 1 hit.
PROSITEiPS01311. LGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLGT_ECOLI
AccessioniPrimary (citable) accession number: P60955
Secondary accession number(s): P37149, Q2MA09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.