P60952 (CDC42_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division control protein 42 homolog

Alternative name(s):
G25K GTP-binding protein

Gene names

Name:

CDC42

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration By similarity.
Enzyme regulation	Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase By similarity.
Subunit structure	Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1 By similarity. Interacts with USP6 By similarity. Interacts with NEK6 By similarity. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Midbody By similarity. Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome By similarity.
Post-translational modification	Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell cycle cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB regulation of attachment of spindle microtubules to kinetochore Inferred from sequence or structural similarity. Source: UniProtKB regulation of filopodium assembly Inferred from sequence or structural similarity. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from sequence or structural similarity. Source: UniProtKB mitotic spindle Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell spindle midzone Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P60952-2)

Also known as: Placental;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P60952-1)

Also known as: Brain;

The sequence of this isoform differs from the canonical sequence as follows:
     163-163: K → R
     182-191: PKKSRRCVLL → TQPKRKCCIF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Cell division control protein 42 homolog		PRO_0000030423
	Propeptide	189 – 191	3	Removed in mature form		PRO_0000030424
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	115 – 118	4	GTP By similarity
	Motif	32 – 40	9	Effector region Potential
Amino acid modifications
	Modified residue	64	1	Phosphotyrosine; by SRC By similarity
	Modified residue	188	1	Cysteine methyl ester By similarity
	Lipidation	188	1	S-geranylgeranyl cysteine By similarity
Natural variations
	Alternative sequence	163	1	K → R in isoform 2.		VSP_010078
	Alternative sequence	182 – 191	10	PKKSRRCVLL → TQPKRKCCIF in isoform 2.		VSP_010079

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Placental) [UniParc]. Last modified February 8, 2011. Version 2. Checksum: 51A437E22A4D8FFF	FASTA	191	21,259
	10 20 30 40 50 60 MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 70 80 90 100 110 120 QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR 130 140 150 160 170 180 DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP 190 EPKKSRRCVL L
	Isoform 2 (Brain) [UniParc] [UniParc]. Checksum: 34B44F9225EC106B	FASTA	191	21,311
	10 20 30 40 50 60 MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 70 80 90 100 110 120 QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR 130 140 150 160 170 180 DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQRGLKNVFD EAILAALEPP 190 ETQPKRKCCI F

References

[1]

"Cytokinesis arrest and redistribution of actin-cytoskeleton regulatory components induced by the Rho GTPase CDC42Hs." Dutartre H., Davoust J., Gorvel J., Chavrier P. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: Cocker spaniel. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49944 mRNA. Translation: CAA90215.1.
PIR	S57563.
RefSeq	NP_001003254.1. NM_001003254.1.
UniGene	Cfa.39956.
3D structure databases
ProteinModelPortal	P60952.
SMR	P60952. Positions 1-191.
ModBase	Search...
Proteomic databases
PaxDb	P60952.
PRIDE	P60952.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000023350; ENSCAFP00000021683; ENSCAFG00000014707. ENSCAFT00000023351; ENSCAFP00000021684; ENSCAFG00000014707.
GeneID	403934.
KEGG	cfa:403934.
Organism-specific databases
CTD	998.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00680000099829.
HOGENOM	HOG000233974.
HOVERGEN	HBG009351.
InParanoid	P60952.
KO	K04393.
OMA	SEYVPTX.
OrthoDB	EOG4R23VV.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P60952.
NextBio	20817424.

Entry information

Entry name

CDC42_CANFA

Accession

Primary (citable) accession number: P60952
Secondary accession number(s): P21181, P25763

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	February 8, 2011
Last modified:	April 3, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program