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Protein

Cell division control protein 42 homolog

Gene

CDC42

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration (By similarity).By similarity

Enzyme regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi115 – 1184GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-182971. EGFR downregulation.
R-CFA-194840. Rho GTPase cycle.
R-CFA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-CFA-375170. CDO in myogenesis.
R-CFA-389359. CD28 dependent Vav1 pathway.
R-CFA-3928662. EPHB-mediated forward signaling.
R-CFA-418885. DCC mediated attractive signaling.
R-CFA-4420097. VEGFA-VEGFR2 Pathway.
R-CFA-5625970. RHO GTPases activate KTN1.
R-CFA-5626467. RHO GTPases activate IQGAPs.
R-CFA-5627123. RHO GTPases activate PAKs.
R-CFA-5663213. RHO GTPases Activate WASPs and WAVEs.
R-CFA-5663220. RHO GTPases Activate Formins.
R-CFA-5687128. MAPK6/MAPK4 signaling.
R-CFA-68877. Mitotic Prometaphase.
R-CFA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 42 homolog
Alternative name(s):
G25K GTP-binding protein
Gene namesi
Name:CDC42
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 2

Subcellular locationi

  • Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Midbody By similarity

  • Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Cell division control protein 42 homologPRO_0000030423Add
BLAST
Propeptidei189 – 1913Removed in mature formPRO_0000030424

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641Phosphotyrosine; by SRCBy similarity
Modified residuei188 – 1881Cysteine methyl esterBy similarity
Lipidationi188 – 1881S-geranylgeranyl cysteineBy similarity

Post-translational modificationi

Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP60952.
PRIDEiP60952.

Interactioni

Subunit structurei

Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1. Interacts with USP6 (By similarity). Interacts with NEK6 (By similarity). Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with ITGB1BP1 (By similarity). Interacts with ARHGDIA; this interaction inactivates and stabilizes CDC42. Interacts with ARHGDIB; this maintains CDC42 in the inactive, GDP-bound form (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1512235.
STRINGi9615.ENSCAFP00000021683.

Structurei

3D structure databases

ProteinModelPortaliP60952.
SMRiP60952. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionSequence analysis

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP60952.
KOiK04393.
OMAiVITIDQG.
OrthoDBiEOG091G0KCM.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60952-2) [UniParc]FASTAAdd to basket
Also known as: Placental

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP
60 70 80 90 100
YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE
110 120 130 140 150
ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK
160 170 180 190
AVKYVECSAL TQKGLKNVFD EAILAALEPP EPKKSRRCVL L
Length:191
Mass (Da):21,259
Last modified:February 8, 2011 - v2
Checksum:i51A437E22A4D8FFF
GO
Isoform 2 (identifier: P60952-1) [UniParc] [UniParc]FASTAAdd to basket
Also known as: Brain

The sequence of this isoform differs from the canonical sequence as follows:
     163-163: K → R
     182-191: PKKSRRCVLL → TQPKRKCCIF

Show »
Length:191
Mass (Da):21,311
Checksum:i34B44F9225EC106B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei163 – 1631K → R in isoform 2. 1 PublicationVSP_010078
Alternative sequencei182 – 19110PKKSRRCVLL → TQPKRKCCIF in isoform 2. 1 PublicationVSP_010079

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49944 mRNA. Translation: CAA90215.1.
PIRiS57563.
RefSeqiNP_001003254.1. NM_001003254.2. [P60952-2]
UniGeneiCfa.39956.

Genome annotation databases

EnsembliENSCAFT00000023350; ENSCAFP00000021683; ENSCAFG00000014707. [P60952-2]
ENSCAFT00000023351; ENSCAFP00000021684; ENSCAFG00000014707. [P60952-1]
GeneIDi403934.
KEGGicfa:403934.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49944 mRNA. Translation: CAA90215.1.
PIRiS57563.
RefSeqiNP_001003254.1. NM_001003254.2. [P60952-2]
UniGeneiCfa.39956.

3D structure databases

ProteinModelPortaliP60952.
SMRiP60952. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1512235.
STRINGi9615.ENSCAFP00000021683.

Proteomic databases

PaxDbiP60952.
PRIDEiP60952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000023350; ENSCAFP00000021683; ENSCAFG00000014707. [P60952-2]
ENSCAFT00000023351; ENSCAFP00000021684; ENSCAFG00000014707. [P60952-1]
GeneIDi403934.
KEGGicfa:403934.

Organism-specific databases

CTDi998.

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP60952.
KOiK04393.
OMAiVITIDQG.
OrthoDBiEOG091G0KCM.
TreeFamiTF101109.

Enzyme and pathway databases

ReactomeiR-CFA-182971. EGFR downregulation.
R-CFA-194840. Rho GTPase cycle.
R-CFA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-CFA-375170. CDO in myogenesis.
R-CFA-389359. CD28 dependent Vav1 pathway.
R-CFA-3928662. EPHB-mediated forward signaling.
R-CFA-418885. DCC mediated attractive signaling.
R-CFA-4420097. VEGFA-VEGFR2 Pathway.
R-CFA-5625970. RHO GTPases activate KTN1.
R-CFA-5626467. RHO GTPases activate IQGAPs.
R-CFA-5627123. RHO GTPases activate PAKs.
R-CFA-5663213. RHO GTPases Activate WASPs and WAVEs.
R-CFA-5663220. RHO GTPases Activate Formins.
R-CFA-5687128. MAPK6/MAPK4 signaling.
R-CFA-68877. Mitotic Prometaphase.
R-CFA-983231. Factors involved in megakaryocyte development and platelet production.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDC42_CANLF
AccessioniPrimary (citable) accession number: P60952
Secondary accession number(s): P21181, P25763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: February 8, 2011
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.