P60952 (CDC42_CANFA) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 93. History...
Names and origin
|Protein names||Recommended name:|
Cell division control protein 42 homolog
G25K GTP-binding protein
|Organism||Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]|
|Taxonomic identifier||9615 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis ›|
|Sequence length||191 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration By similarity.
Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase By similarity.
Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1 By similarity. Interacts with USP6 By similarity. Interacts with NEK6 By similarity. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas By similarity.
Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Midbody By similarity. Note: Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome By similarity.
Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI By similarity.
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform 1 (identifier: P60952-2) |
Also known as: Placental;
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: P60952-1) |
Also known as: Brain;
The sequence of this isoform differs from the canonical sequence as follows:
163-163: K → R
182-191: PKKSRRCVLL → TQPKRKCCIF
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 188||188||Cell division control protein 42 homolog||PRO_0000030423|
|Propeptide||189 – 191||3||Removed in mature form||PRO_0000030424|
|Nucleotide binding||10 – 17||8||GTP By similarity|
|Nucleotide binding||57 – 61||5||GTP By similarity|
|Nucleotide binding||115 – 118||4||GTP By similarity|
|Motif||32 – 40||9||Effector region Potential|
Amino acid modifications
|Modified residue||64||1||Phosphotyrosine; by SRC By similarity|
|Modified residue||188||1||Cysteine methyl ester By similarity|
|Lipidation||188||1||S-geranylgeranyl cysteine By similarity|
|Alternative sequence||163||1||K → R in isoform 2.||VSP_010078|
|Alternative sequence||182 – 191||10||PKKSRRCVLL → TQPKRKCCIF in isoform 2.||VSP_010079|
|||"Cytokinesis arrest and redistribution of actin-cytoskeleton regulatory components induced by the Rho GTPase CDC42Hs."|
Dutartre H., Davoust J., Gorvel J., Chavrier P.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: Cocker spaniel.
|Z49944 mRNA. Translation: CAA90215.1.|
|RefSeq||NP_001003254.1. NM_001003254.1. |
3D structure databases
|SMR||P60952. Positions 1-191. |
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSCAFT00000023350; ENSCAFP00000021683; ENSCAFG00000014707. |
ENSCAFT00000023351; ENSCAFP00000021684; ENSCAFG00000014707.
Family and domain databases
|InterPro||IPR005225. Small_GTP-bd_dom. |
|Pfam||PF00071. Ras. 1 hit. |
|PRINTS||PR00449. RASTRNSFRMNG. |
|SMART||SM00174. RHO. 1 hit. |
|TIGRFAMs||TIGR00231. small_GTP. 1 hit. |
|PROSITE||PS51420. RHO. 1 hit. |
|Accession||Primary (citable) accession number: P60952|
Secondary accession number(s): P21181, P25763
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families