P60932 (UPPP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Undecaprenyl-diphosphatase
EC=3.6.1.27

Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase

Gene names

Name:	uppP
Synonyms:	bacA, upk
Ordered Locus Names:	b3057, JW3029

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin. Ref.7
Catalytic activity	Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. Ref.6
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.6 Ref.8.
Miscellaneous	Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available. HAMAP-Rule MF_01006
Sequence similarities	Belongs to the UppP family.
Caution	Was originally (Ref.3) thought to be an undecaprenol kinase.
Sequence caution	The sequence L12966 differs from that shown. Reason: Frameshift at position 145. The sequence M12788 differs from that shown. Reason: Frameshift at positions 177 and 210.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	undecaprenyl-diphosphatase activity Inferred from direct assay Ref.6. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Undecaprenyl-diphosphatase HAMAP-Rule MF_01006		PRO_0000151146
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	190 – 210	21	Helical; Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Transmembrane	252 – 272	21	Helical; Potential
Experimental info
	Sequence conflict	158	1	F → I Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P60932 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 6BFAC099D5163352	FASTA	273	29,759
	10 20 30 40 50 60 MSDMHSLLIA AILGVVEGLT EFLPVSSTGH MIIVGHLLGF EGDTAKTFEV VIQLGSILAV 70 80 90 100 110 120 VVMFWRRLFG LIGIHFGRPL QHEGESKGRL TLIHILLGMI PAVVLGLLFH DTIKSLFNPI 130 140 150 160 170 180 NVMYALVVGG LLLIAAECLK PKEPRAPGLD DMTYRQAFMI GCFQCLALWP GFSRSGATIS 190 200 210 220 230 240 GGMLMGVSRY AASEFSFLLA VPMMMGATAL DLYKSWGFLT SGDIPMFAVG FITAFVVALI 250 260 270 AIKTFLQLIK RISFIPFAIY RFIVAAAVYV VFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Amplification of the bacA gene confers bacitracin resistance to Escherichia coli." Cain B.D., Norton P.J., Eubanks W., Nick H.S., Allen C.M. J. Bacteriol. 175:3784-3789(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157, RESISTANCE TO BACITRACIN. Strain: K12 / ATCC 35607 / JM83.
[4]	"Cloning, sequencing, and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidyltransferase." Cudny H., Lupski J.R., Godson G.N., Deutscher M.P. J. Biol. Chem. 261:6444-6449(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-273.
[5]	Rudd K.E. Unpublished observations (NOV-1994) Cited for: PRELIMINARY CONCEPTUAL TRANSLATION.
[6]	"The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity." El Ghachi M., Bouhss A., Blanot D., Mengin-Lecreulx D. J. Biol. Chem. 279:30106-30113(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[7]	"Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli." El Ghachi M., Derbise A., Bouhss A., Mengin-Lecreulx D. J. Biol. Chem. 280:18689-18695(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28379 Genomic DNA. Translation: AAA89137.1. U00096 Genomic DNA. Translation: AAC76093.1. AP009048 Genomic DNA. Translation: BAE77108.1. L12966 Genomic DNA. No translation available. M12788 Genomic DNA. No translation available.
PIR	G65093.
RefSeq	NP_417529.1. NC_000913.2. YP_491249.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P60932.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48044N.
IntAct	P60932. 1 interaction.
STRING	511145.b3057.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76093; AAC76093; b3057. BAE77108; BAE77108; BAE77108.
GeneID	12932196. 947551.
KEGG	ecj:Y75_p2983. eco:b3057.
PATRIC	32121526. VBIEscCol129921_3150.
Organism-specific databases
EchoBASE	EB1616.
EcoGene	EG11665. uppP.
Phylogenomic databases
eggNOG	COG1968.
HOGENOM	HOG000218356.
KO	K06153.
OMA	LQIGLFQ.
ProtClustDB	PRK00281.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11665-MONOMER. ECOL316407:JW3029-MONOMER. MetaCyc:EG11665-MONOMER.
Gene expression databases
Genevestigator	P60932.
Family and domain databases
HAMAP	MF_01006. Undec_diphosphatase.
InterPro	IPR003824. Bacitracin-R_BacA. [Graphical view]
Pfam	PF02673. BacA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00753. undec_PP_bacA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPPP_ECOLI

Accession

Primary (citable) accession number: P60932
Secondary accession number(s): P31054, P39203, Q2M9E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program