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P60932 (UPPP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Undecaprenyl-diphosphatase

EC=3.6.1.27
Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase
Gene names
Name:uppP
Synonyms:bacA, upk
Ordered Locus Names:b3057, JW3029
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin. Ref.7

Catalytic activity

Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. Ref.6

Subcellular location

Cell inner membrane; Multi-pass membrane protein Ref.6 Ref.8.

Miscellaneous

Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.

Sequence similarities

Belongs to the UppP family.

Caution

Was originally (Ref.3) thought to be an undecaprenol kinase.

Sequence caution

The sequence L12966 differs from that shown. Reason: Frameshift at position 145.

The sequence M12788 differs from that shown. Reason: Frameshift at positions 177 and 210.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Undecaprenyl-diphosphatase HAMAP-Rule MF_01006
PRO_0000151146

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane45 – 6521Helical; Potential
Transmembrane90 – 11021Helical; Potential
Transmembrane116 – 13621Helical; Potential
Transmembrane190 – 21021Helical; Potential
Transmembrane222 – 24221Helical; Potential
Transmembrane252 – 27221Helical; Potential

Experimental info

Sequence conflict1581F → I Ref.4

Sequences

Sequence LengthMass (Da)Tools
P60932 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 6BFAC099D5163352

FASTA27329,759
        10         20         30         40         50         60 
MSDMHSLLIA AILGVVEGLT EFLPVSSTGH MIIVGHLLGF EGDTAKTFEV VIQLGSILAV 

        70         80         90        100        110        120 
VVMFWRRLFG LIGIHFGRPL QHEGESKGRL TLIHILLGMI PAVVLGLLFH DTIKSLFNPI 

       130        140        150        160        170        180 
NVMYALVVGG LLLIAAECLK PKEPRAPGLD DMTYRQAFMI GCFQCLALWP GFSRSGATIS 

       190        200        210        220        230        240 
GGMLMGVSRY AASEFSFLLA VPMMMGATAL DLYKSWGFLT SGDIPMFAVG FITAFVVALI 

       250        260        270 
AIKTFLQLIK RISFIPFAIY RFIVAAAVYV VFF 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Amplification of the bacA gene confers bacitracin resistance to Escherichia coli."
Cain B.D., Norton P.J., Eubanks W., Nick H.S., Allen C.M.
J. Bacteriol. 175:3784-3789(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157, RESISTANCE TO BACITRACIN.
Strain: K12 / ATCC 35607 / JM83.
[4]"Cloning, sequencing, and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidyltransferase."
Cudny H., Lupski J.R., Godson G.N., Deutscher M.P.
J. Biol. Chem. 261:6444-6449(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-273.
[5]Rudd K.E.
Unpublished observations (NOV-1994)
Cited for: PRELIMINARY CONCEPTUAL TRANSLATION.
[6]"The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity."
El Ghachi M., Bouhss A., Blanot D., Mengin-Lecreulx D.
J. Biol. Chem. 279:30106-30113(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[7]"Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli."
El Ghachi M., Derbise A., Bouhss A., Mengin-Lecreulx D.
J. Biol. Chem. 280:18689-18695(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28379 Genomic DNA. Translation: AAA89137.1.
U00096 Genomic DNA. Translation: AAC76093.1.
AP009048 Genomic DNA. Translation: BAE77108.1.
L12966 Genomic DNA. No translation available.
M12788 Genomic DNA. No translation available.
PIRG65093.
RefSeqNP_417529.1. NC_000913.3.
YP_491249.1. NC_007779.1.

3D structure databases

ProteinModelPortalP60932.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48044N.
IntActP60932. 1 interaction.
STRING511145.b3057.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76093; AAC76093; b3057.
BAE77108; BAE77108; BAE77108.
GeneID12932196.
947551.
KEGGecj:Y75_p2983.
eco:b3057.
PATRIC32121526. VBIEscCol129921_3150.

Organism-specific databases

EchoBASEEB1616.
EcoGeneEG11665. uppP.

Phylogenomic databases

eggNOGCOG1968.
HOGENOMHOG000218356.
KOK06153.
OMAAQCFALI.
OrthoDBEOG6QP13M.
PhylomeDBP60932.
ProtClustDBPRK00281.

Enzyme and pathway databases

BioCycEcoCyc:EG11665-MONOMER.
ECOL316407:JW3029-MONOMER.
MetaCyc:EG11665-MONOMER.

Gene expression databases

GenevestigatorP60932.

Family and domain databases

HAMAPMF_01006. Undec_diphosphatase.
InterProIPR003824. UppP.
[Graphical view]
PfamPF02673. BacA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00753. undec_PP_bacA. 1 hit.
ProtoNetSearch...

Other

PROP60932.

Entry information

Entry nameUPPP_ECOLI
AccessionPrimary (citable) accession number: P60932
Secondary accession number(s): P31054, P39203, Q2M9E8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene