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P60932

- UPPP_ECOLI

UniProt

P60932 - UPPP_ECOLI

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Protein

Undecaprenyl-diphosphatase

Gene

uppP

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.1 Publication

Catalytic activityi

Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate.1 Publication

GO - Molecular functioni

  1. undecaprenyl-diphosphatase activity Source: EcoliWiki

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. dephosphorylation Source: InterPro
  3. peptidoglycan biosynthetic process Source: UniProtKB-KW
  4. regulation of cell shape Source: UniProtKB-KW
  5. response to antibiotic Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11665-MONOMER.
ECOL316407:JW3029-MONOMER.
MetaCyc:EG11665-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl-diphosphatase (EC:3.6.1.27)
Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase
Gene namesi
Name:uppP
Synonyms:bacA, upk
Ordered Locus Names:b3057, JW3029
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11665. uppP.

Subcellular locationi

Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Undecaprenyl-diphosphatasePRO_0000151146Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP60932.

Interactioni

Protein-protein interaction databases

DIPiDIP-48044N.
IntActiP60932. 1 interaction.
STRINGi511145.b3057.

Structurei

3D structure databases

ProteinModelPortaliP60932.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence AnalysisAdd
BLAST
Transmembranei45 – 6521HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence AnalysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence AnalysisAdd
BLAST
Transmembranei190 – 21021HelicalSequence AnalysisAdd
BLAST
Transmembranei222 – 24221HelicalSequence AnalysisAdd
BLAST
Transmembranei252 – 27221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the UppP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1968.
HOGENOMiHOG000218356.
InParanoidiP60932.
KOiK06153.
OMAiFNDAHAK.
OrthoDBiEOG6QP13M.
PhylomeDBiP60932.

Family and domain databases

HAMAPiMF_01006. Undec_diphosphatase.
InterProiIPR003824. UppP.
[Graphical view]
PfamiPF02673. BacA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00753. undec_PP_bacA. 1 hit.

Sequencei

Sequence statusi: Complete.

P60932-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDMHSLLIA AILGVVEGLT EFLPVSSTGH MIIVGHLLGF EGDTAKTFEV
60 70 80 90 100
VIQLGSILAV VVMFWRRLFG LIGIHFGRPL QHEGESKGRL TLIHILLGMI
110 120 130 140 150
PAVVLGLLFH DTIKSLFNPI NVMYALVVGG LLLIAAECLK PKEPRAPGLD
160 170 180 190 200
DMTYRQAFMI GCFQCLALWP GFSRSGATIS GGMLMGVSRY AASEFSFLLA
210 220 230 240 250
VPMMMGATAL DLYKSWGFLT SGDIPMFAVG FITAFVVALI AIKTFLQLIK
260 270
RISFIPFAIY RFIVAAAVYV VFF
Length:273
Mass (Da):29,759
Last modified:April 13, 2004 - v1
Checksum:i6BFAC099D5163352
GO

Sequence cautioni

The sequence L12966 differs from that shown. Reason: Frameshift at position 145.
The sequence M12788 differs from that shown. Reason: Frameshift at positions 177 and 210.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581F → I(PubMed:3009457)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28379 Genomic DNA. Translation: AAA89137.1.
U00096 Genomic DNA. Translation: AAC76093.1.
AP009048 Genomic DNA. Translation: BAE77108.1.
L12966 Genomic DNA. No translation available.
M12788 Genomic DNA. No translation available.
PIRiG65093.
RefSeqiNP_417529.1. NC_000913.3.
YP_491249.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76093; AAC76093; b3057.
BAE77108; BAE77108; BAE77108.
GeneIDi12932196.
947551.
KEGGiecj:Y75_p2983.
eco:b3057.
PATRICi32121526. VBIEscCol129921_3150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28379 Genomic DNA. Translation: AAA89137.1 .
U00096 Genomic DNA. Translation: AAC76093.1 .
AP009048 Genomic DNA. Translation: BAE77108.1 .
L12966 Genomic DNA. No translation available.
M12788 Genomic DNA. No translation available.
PIRi G65093.
RefSeqi NP_417529.1. NC_000913.3.
YP_491249.1. NC_007779.1.

3D structure databases

ProteinModelPortali P60932.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48044N.
IntActi P60932. 1 interaction.
STRINGi 511145.b3057.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76093 ; AAC76093 ; b3057 .
BAE77108 ; BAE77108 ; BAE77108 .
GeneIDi 12932196.
947551.
KEGGi ecj:Y75_p2983.
eco:b3057.
PATRICi 32121526. VBIEscCol129921_3150.

Organism-specific databases

EchoBASEi EB1616.
EcoGenei EG11665. uppP.

Phylogenomic databases

eggNOGi COG1968.
HOGENOMi HOG000218356.
InParanoidi P60932.
KOi K06153.
OMAi FNDAHAK.
OrthoDBi EOG6QP13M.
PhylomeDBi P60932.

Enzyme and pathway databases

BioCyci EcoCyc:EG11665-MONOMER.
ECOL316407:JW3029-MONOMER.
MetaCyc:EG11665-MONOMER.

Miscellaneous databases

PROi P60932.

Gene expression databases

Genevestigatori P60932.

Family and domain databases

HAMAPi MF_01006. Undec_diphosphatase.
InterProi IPR003824. UppP.
[Graphical view ]
Pfami PF02673. BacA. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00753. undec_PP_bacA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Amplification of the bacA gene confers bacitracin resistance to Escherichia coli."
    Cain B.D., Norton P.J., Eubanks W., Nick H.S., Allen C.M.
    J. Bacteriol. 175:3784-3789(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157, RESISTANCE TO BACITRACIN.
    Strain: K12 / ATCC 35607 / JM83.
  4. "Cloning, sequencing, and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidyltransferase."
    Cudny H., Lupski J.R., Godson G.N., Deutscher M.P.
    J. Biol. Chem. 261:6444-6449(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-273.
  5. Rudd K.E.
    Unpublished observations (NOV-1994)
    Cited for: PRELIMINARY CONCEPTUAL TRANSLATION.
  6. "The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity."
    El Ghachi M., Bouhss A., Blanot D., Mengin-Lecreulx D.
    J. Biol. Chem. 279:30106-30113(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  7. "Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli."
    El Ghachi M., Derbise A., Bouhss A., Mengin-Lecreulx D.
    J. Biol. Chem. 280:18689-18695(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiUPPP_ECOLI
AccessioniPrimary (citable) accession number: P60932
Secondary accession number(s): P31054, P39203, Q2M9E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.

Caution

Was originally thought to be an undecaprenol kinase.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3