Undecaprenyl-diphosphatase - Escherichia coli (strain K12)

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P60932 (UPPP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Undecaprenyl-diphosphatase
EC=3.6.1.27

Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase

Gene names

Name:	uppP
Synonyms:	bacA, upk
Ordered Locus Names:	b3057, JW3029

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin. Ref.7
Catalytic activity	Undecaprenyl diphosphate + H₂O = undecaprenyl phosphate + phosphate. Ref.6
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.6 Ref.8.
Miscellaneous	Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available. HAMAP MF_01006
Sequence similarities	Belongs to the UppP family.
Caution	Was originally (Ref.3) thought to be an undecaprenol kinase.
Sequence caution	The sequence L12966 differs from that shown. Reason: Frameshift at position 145. The sequence M12788 differs from that shown. Reason: Frameshift at positions 177 and 210.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW dephosphorylation Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	undecaprenyl-diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 273

273

Undecaprenyl-diphosphatase HAMAP MF_01006

PRO_0000151146

Regions

Transmembrane

6 – 26

Helical; Potential

Transmembrane

45 – 65

Helical; Potential

Transmembrane

90 – 110

Helical; Potential

Transmembrane

116 – 136

Helical; Potential

Transmembrane

190 – 210

Helical; Potential

Transmembrane

222 – 242

Helical; Potential

Transmembrane

252 – 272

Helical; Potential

Experimental info

Sequence conflict

158

F → I Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P60932 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 6BFAC099D5163352
Show »

FASTA

273

29,759

        10         20         30         40         50         60 
MSDMHSLLIA AILGVVEGLT EFLPVSSTGH MIIVGHLLGF EGDTAKTFEV VIQLGSILAV 

        70         80         90        100        110        120 
VVMFWRRLFG LIGIHFGRPL QHEGESKGRL TLIHILLGMI PAVVLGLLFH DTIKSLFNPI 

       130        140        150        160        170        180 
NVMYALVVGG LLLIAAECLK PKEPRAPGLD DMTYRQAFMI GCFQCLALWP GFSRSGATIS 

       190        200        210        220        230        240 
GGMLMGVSRY AASEFSFLLA VPMMMGATAL DLYKSWGFLT SGDIPMFAVG FITAFVVALI 

       250        260        270 
AIKTFLQLIK RISFIPFAIY RFIVAAAVYV VFF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Amplification of the bacA gene confers bacitracin resistance to Escherichia coli." Cain B.D., Norton P.J., Eubanks W., Nick H.S., Allen C.M. J. Bacteriol. 175:3784-3789(1993) [PubMed: 8389741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157, RESISTANCE TO BACITRACIN. Strain: K12 / ATCC 35607 / JM83.
[4]	"Cloning, sequencing, and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidyltransferase." Cudny H., Lupski J.R., Godson G.N., Deutscher M.P. J. Biol. Chem. 261:6444-6449(1986) [PubMed: 3009457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-273.
[5]	Rudd K.E. Unpublished observations (NOV-1994) Cited for: PRELIMINARY CONCEPTUAL TRANSLATION.
[6]	"The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity." El Ghachi M., Bouhss A., Blanot D., Mengin-Lecreulx D. J. Biol. Chem. 279:30106-30113(2004) [PubMed: 15138271] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[7]	"Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli." El Ghachi M., Derbise A., Bouhss A., Mengin-Lecreulx D. J. Biol. Chem. 280:18689-18695(2005) [PubMed: 15778224] [Abstract] Cited for: FUNCTION.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28379 Genomic DNA. Translation: AAA89137.1. U00096 Genomic DNA. Translation: AAC76093.1. AP009048 Genomic DNA. Translation: BAE77108.1. L12966 Genomic DNA. No translation available. M12788 Genomic DNA. No translation available.
PIR	G65093.
RefSeq	NP_417529.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P60932.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48044N.
IntAct	P60932. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001504; EBESCP00000001504; EBESCG00000001250. EBESCT00000015069; EBESCP00000014360; EBESCG00000014129.
GeneID	947551.
GenomeReviews	Gene locus JW3029 in contig AP009048_GR. Gene locus b3057 in contig U00096_GR.
KEGG	ecj:JW3029. eco:b3057.
PATRIC	32121526. VBIEscCol129921_3150.
Organism-specific databases
EchoBASE	EB1616.
EcoGene	EG11665. uppP.
Phylogenomic databases
eggNOG	COG1968.
GeneTree	EBGT00050000011219.
HOGENOM	HBG391969.
OMA	IAFMPAV.
PhylomeDB	P60932.
ProtClustDB	PRK00281.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11665-MONOMER. MetaCyc:EG11665-MONOMER.
Gene expression databases
Genevestigator	P60932.
Family and domain databases
HAMAP	MF_01006. Undec_diphosphatase. [Tree]
InterPro	IPR003824. Bacitracin-R_BacA. [Graphical view]
KO	K06153.
Pfam	PF02673. BacA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00753. Undec_PP_bacA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPPP_ECOLI

Accession

Primary (citable) accession number: P60932
Secondary accession number(s): P31054, P39203, Q2M9E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents