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Reviewed, UniProtKB/Swiss-Prot P60906 (SYH_ECOLI)

Last modified June 16, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Histidyl-tRNA synthetase
    EC=6.1.1.21
Alternative name(s):
    Histidine--tRNA ligase
      Short name=HisRS
Gene names
Name: hisS
Ordered Locus Names: b2514, JW2498
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127

Subunit structure

Homodimer. HAMAP MF_00127

Subcellular location

Cytoplasm. HAMAP MF_00127

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processhistidyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

histidine-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed HAMAP MF_00127
Chain2 – 424423Histidyl-tRNA synthetase HAMAP MF_00127
PRO_0000136161

Secondary structure

.............................................................. 424
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60906-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: EBBA69DE488CB00D

FASTA42447,029
        10         20         30         40         50         60 
MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP LFKRAIGEVT 

        70         80         90        100        110        120 
DVVEKEMYTF EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN QEQRLWYIGP MFRHERPQKG 

       130        140        150        160        170        180 
RYRQFHQLGC EVFGLQGPDI DAELIMLTAR WWRALGISEH VTLELNSIGS LEARANYRDA 

       190        200        210        220        230        240 
LVAFLEQHKE KLDEDCKRRM YTNPLRVLDS KNPEVQALLN DAPALGDYLD EESREHFAGL 

       250        260        270        280        290        300 
CKLLESAGIA YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG TVCAGGRYDG LVEQLGGRAT 

       310        320        330        340        350        360 
PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER LRDELPGVKL 

       370        380        390        400        410        420 
MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK DLRSGEQTAV AQDSVAAHLR 


TLLG 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of histidine-tRNA synthetase and characterization of hisS transcripts."
Freedman R., Gibson B., Donovan D., Biemann K., Eisenbeis S.J., Parker J., Schimmel P.
J. Biol. Chem. 260:10063-10068(1985) [PubMed: 2991272] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The nucleotide sequence of the promoter region of hisS, the structural gene for histidyl-tRNA synthetase."
Eisenbeis S.J., Parker J.
Gene 18:107-114(1982) [PubMed: 6290315] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
[6]"Crystal structure of histidyl-tRNA synthetase from Escherichia coli complexed with histidyl-adenylate."
Arnez J.G., Harris D.C., Mitschler A., Rees B., Francklyn C.S., Moras D.
EMBO J. 14:4143-4155(1995) [PubMed: 7556055] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
[7]"The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase."
Arnez J.G., Augustine J.G., Moras D., Francklyn C.S.
Proc. Natl. Acad. Sci. U.S.A. 94:7144-7149(1997) [PubMed: 9207058] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

Cross-references

Sequence databases

M11843 Genomic DNA. Translation: AAA03226.1.
U00096 Genomic DNA. Translation: AAC75567.1.
AP009048 Genomic DNA. Translation: BAA16401.1.
PIRSYECH. A23890.
RefSeqAP_003100.1.
NP_417009.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1HTTX-ray2.60A/B/C/D1-424[»]
1KMMX-ray2.60A/B/C/D1-424[»]
1KMNX-ray2.80A/B/C/D1-424[»]
2EL9X-ray2.70A/B/C/D1-424[»]
ModBaseSearch...

2-D gel databases

SWISS-2DPAGEP60906.
ECO2DBASEF048.1. 6TH EDITION.

Genome annotation databases

GeneID946989.
GenomeReviewsGene locus JW2498 in contig AP009048_GR.
Gene locus b2514 in contig U00096_GR.
KEGGecj:JW2498.
eco:b2514.

Organism-specific databases

EchoBASEEB0448.
EcoGeneEG10453. hisS.
CMRSearch...

Phylogenomic databases

HOGENOMP60906.
OMAP60906. VFEWVTT.

Enzyme and pathway databases

BioCycEcoCyc:HISS-MON.
MetaCyc:HISS-MON.

Family and domain databases

HAMAPMF_00127.
[Tree]
InterProIPR002314. aa-tRNA-synt_IIb_cons-reg.
IPR006195. aa-tRNA-synth_II_cons-reg.
IPR004154. Anticodon_bd.
IPR015807. His-tRNA-synth_IIa_sub.
IPR004516. His-tRNA_synth_IIA.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHERPTHR11476. His-tRNA_synth. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFPIRSF001549. His-tRNA_synth. 1 hit.
TIGRFAMsTIGR00442. hisS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00131. Adenosine monophosphate.

Entry information

Entry nameSYH_ECOLI
AccessionPrimary (citable) accession number: P60906
Secondary accession number(s): P04804
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents