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Protein

Histidine--tRNA ligase

Gene

hisS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine-tRNA ligase activity Source: EcoCyc

GO - Biological processi

  • histidyl-tRNA aminoacylation Source: EcoCyc

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HISS-MONOMER
MetaCyc:HISS-MONOMER
BRENDAi6.1.1.21 2026
SABIO-RKP60906

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:hisS
Ordered Locus Names:b2514, JW2498
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10453 hisS

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB03811 Histidinol
DB04201 Histidyl-Adenosine Monophosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001361612 – 424Histidine--tRNA ligaseAdd BLAST423

Proteomic databases

EPDiP60906
PaxDbiP60906
PRIDEiP60906

2D gel databases

SWISS-2DPAGEP60906

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4260589, 66 interactors
DIPiDIP-35894N
IntActiP60906, 2 interactors
STRINGi316385.ECDH10B_2680

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 34Combined sources19
Turni35 – 37Combined sources3
Beta strandi45 – 48Combined sources4
Helixi49 – 56Combined sources8
Beta strandi58 – 60Combined sources3
Helixi61 – 65Combined sources5
Beta strandi68 – 71Combined sources4
Beta strandi77 – 80Combined sources4
Helixi85 – 95Combined sources11
Beta strandi103 – 112Combined sources10
Beta strandi123 – 134Combined sources12
Helixi138 – 154Combined sources17
Helixi158 – 160Combined sources3
Beta strandi162 – 167Combined sources6
Helixi171 – 176Combined sources6
Helixi178 – 182Combined sources5
Helixi225 – 227Combined sources3
Helixi231 – 245Combined sources15
Turni246 – 248Combined sources3
Beta strandi251 – 253Combined sources3
Beta strandi260 – 262Combined sources3
Beta strandi266 – 272Combined sources7
Turni277 – 279Combined sources3
Beta strandi281 – 287Combined sources7
Helixi291 – 294Combined sources4
Beta strandi302 – 308Combined sources7
Helixi309 – 319Combined sources11
Beta strandi320 – 322Combined sources3
Beta strandi330 – 335Combined sources6
Helixi340 – 354Combined sources15
Beta strandi360 – 362Combined sources3
Helixi369 – 379Combined sources11
Beta strandi382 – 387Combined sources6
Helixi389 – 394Combined sources6
Beta strandi396 – 401Combined sources6
Turni402 – 404Combined sources3
Beta strandi407 – 411Combined sources5
Helixi412 – 423Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HTTX-ray2.60A/B/C/D2-424[»]
1KMMX-ray2.60A/B/C/D1-424[»]
1KMNX-ray2.80A/B/C/D1-424[»]
2EL9X-ray2.70A/B/C/D1-424[»]
ProteinModelPortaliP60906
SMRiP60906
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60906

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CUT Bacteria
COG0124 LUCA
HOGENOMiHOG000018072
InParanoidiP60906
KOiK01892
OMAiCDFDFIG
PhylomeDBiP60906

Family and domain databases

CDDicd00859 HisRS_anticodon, 1 hit
Gene3Di3.40.50.800, 1 hit
HAMAPiMF_00127 His_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR015807 His-tRNA-ligase
IPR004516 HisRS/HisZ
IPR033656 HisRS_anticodon
PANTHERiPTHR43707 PTHR43707, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PIRSFiPIRSF001549 His-tRNA_synth, 1 hit
TIGRFAMsiTIGR00442 hisS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP
60 70 80 90 100
LFKRAIGEVT DVVEKEMYTF EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN
110 120 130 140 150
QEQRLWYIGP MFRHERPQKG RYRQFHQLGC EVFGLQGPDI DAELIMLTAR
160 170 180 190 200
WWRALGISEH VTLELNSIGS LEARANYRDA LVAFLEQHKE KLDEDCKRRM
210 220 230 240 250
YTNPLRVLDS KNPEVQALLN DAPALGDYLD EESREHFAGL CKLLESAGIA
260 270 280 290 300
YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG TVCAGGRYDG LVEQLGGRAT
310 320 330 340 350
PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER
360 370 380 390 400
LRDELPGVKL MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK
410 420
DLRSGEQTAV AQDSVAAHLR TLLG
Length:424
Mass (Da):47,029
Last modified:January 23, 2007 - v2
Checksum:iEBBA69DE488CB00D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11843 Genomic DNA Translation: AAA03226.1
U00096 Genomic DNA Translation: AAC75567.1
AP009048 Genomic DNA Translation: BAA16401.1
PIRiA23890 SYECH
RefSeqiNP_417009.1, NC_000913.3
WP_001107167.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75567; AAC75567; b2514
BAA16401; BAA16401; BAA16401
GeneIDi946989
KEGGiecj:JW2498
eco:b2514
PATRICifig|1411691.4.peg.4222

Similar proteinsi

Entry informationi

Entry nameiSYH_ECOLI
AccessioniPrimary (citable) accession number: P60906
Secondary accession number(s): P04804
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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