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Protein

Histidine--tRNA ligase

Gene

hisS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • histidine-tRNA ligase activity Source: EcoCyc

GO - Biological processi

  • histidyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HISS-MONOMER.
ECOL316407:JW2498-MONOMER.
MetaCyc:HISS-MONOMER.
BRENDAi6.1.1.21. 2026.
SABIO-RKP60906.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:hisS
Ordered Locus Names:b2514, JW2498
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10453. hisS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001361612 – 424Histidine--tRNA ligaseAdd BLAST423

Proteomic databases

EPDiP60906.
PaxDbiP60906.
PRIDEiP60906.

2D gel databases

SWISS-2DPAGEP60906.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4260589. 29 interactors.
DIPiDIP-35894N.
IntActiP60906. 2 interactors.
MINTiMINT-1236095.
STRINGi511145.b2514.

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 34Combined sources19
Turni35 – 37Combined sources3
Beta strandi45 – 48Combined sources4
Helixi49 – 56Combined sources8
Beta strandi58 – 60Combined sources3
Helixi61 – 65Combined sources5
Beta strandi68 – 71Combined sources4
Beta strandi77 – 80Combined sources4
Helixi85 – 95Combined sources11
Beta strandi103 – 112Combined sources10
Beta strandi123 – 134Combined sources12
Helixi138 – 154Combined sources17
Helixi158 – 160Combined sources3
Beta strandi162 – 167Combined sources6
Helixi171 – 176Combined sources6
Helixi178 – 182Combined sources5
Helixi225 – 227Combined sources3
Helixi231 – 245Combined sources15
Turni246 – 248Combined sources3
Beta strandi251 – 253Combined sources3
Beta strandi260 – 262Combined sources3
Beta strandi266 – 272Combined sources7
Turni277 – 279Combined sources3
Beta strandi281 – 287Combined sources7
Helixi291 – 294Combined sources4
Beta strandi302 – 308Combined sources7
Helixi309 – 319Combined sources11
Beta strandi320 – 322Combined sources3
Beta strandi330 – 335Combined sources6
Helixi340 – 354Combined sources15
Beta strandi360 – 362Combined sources3
Helixi369 – 379Combined sources11
Beta strandi382 – 387Combined sources6
Helixi389 – 394Combined sources6
Beta strandi396 – 401Combined sources6
Turni402 – 404Combined sources3
Beta strandi407 – 411Combined sources5
Helixi412 – 423Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HTTX-ray2.60A/B/C/D2-424[»]
1KMMX-ray2.60A/B/C/D1-424[»]
1KMNX-ray2.80A/B/C/D1-424[»]
2EL9X-ray2.70A/B/C/D1-424[»]
ProteinModelPortaliP60906.
SMRiP60906.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60906.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CUT. Bacteria.
COG0124. LUCA.
HOGENOMiHOG000018072.
InParanoidiP60906.
KOiK01892.
OMAiCGGGNFK.
PhylomeDBiP60906.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP
60 70 80 90 100
LFKRAIGEVT DVVEKEMYTF EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN
110 120 130 140 150
QEQRLWYIGP MFRHERPQKG RYRQFHQLGC EVFGLQGPDI DAELIMLTAR
160 170 180 190 200
WWRALGISEH VTLELNSIGS LEARANYRDA LVAFLEQHKE KLDEDCKRRM
210 220 230 240 250
YTNPLRVLDS KNPEVQALLN DAPALGDYLD EESREHFAGL CKLLESAGIA
260 270 280 290 300
YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG TVCAGGRYDG LVEQLGGRAT
310 320 330 340 350
PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER
360 370 380 390 400
LRDELPGVKL MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK
410 420
DLRSGEQTAV AQDSVAAHLR TLLG
Length:424
Mass (Da):47,029
Last modified:January 23, 2007 - v2
Checksum:iEBBA69DE488CB00D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11843 Genomic DNA. Translation: AAA03226.1.
U00096 Genomic DNA. Translation: AAC75567.1.
AP009048 Genomic DNA. Translation: BAA16401.1.
PIRiA23890. SYECH.
RefSeqiNP_417009.1. NC_000913.3.
WP_001107167.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75567; AAC75567; b2514.
BAA16401; BAA16401; BAA16401.
GeneIDi946989.
KEGGiecj:JW2498.
eco:b2514.
PATRICi32120421. VBIEscCol129921_2613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11843 Genomic DNA. Translation: AAA03226.1.
U00096 Genomic DNA. Translation: AAC75567.1.
AP009048 Genomic DNA. Translation: BAA16401.1.
PIRiA23890. SYECH.
RefSeqiNP_417009.1. NC_000913.3.
WP_001107167.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HTTX-ray2.60A/B/C/D2-424[»]
1KMMX-ray2.60A/B/C/D1-424[»]
1KMNX-ray2.80A/B/C/D1-424[»]
2EL9X-ray2.70A/B/C/D1-424[»]
ProteinModelPortaliP60906.
SMRiP60906.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260589. 29 interactors.
DIPiDIP-35894N.
IntActiP60906. 2 interactors.
MINTiMINT-1236095.
STRINGi511145.b2514.

2D gel databases

SWISS-2DPAGEP60906.

Proteomic databases

EPDiP60906.
PaxDbiP60906.
PRIDEiP60906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75567; AAC75567; b2514.
BAA16401; BAA16401; BAA16401.
GeneIDi946989.
KEGGiecj:JW2498.
eco:b2514.
PATRICi32120421. VBIEscCol129921_2613.

Organism-specific databases

EchoBASEiEB0448.
EcoGeneiEG10453. hisS.

Phylogenomic databases

eggNOGiENOG4105CUT. Bacteria.
COG0124. LUCA.
HOGENOMiHOG000018072.
InParanoidiP60906.
KOiK01892.
OMAiCGGGNFK.
PhylomeDBiP60906.

Enzyme and pathway databases

BioCyciEcoCyc:HISS-MONOMER.
ECOL316407:JW2498-MONOMER.
MetaCyc:HISS-MONOMER.
BRENDAi6.1.1.21. 2026.
SABIO-RKP60906.

Miscellaneous databases

EvolutionaryTraceiP60906.
PROiP60906.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00127. His_tRNA_synth. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYH_ECOLI
AccessioniPrimary (citable) accession number: P60906
Secondary accession number(s): P04804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.