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Protein

DnaJ homolog subfamily C member 5

Gene

Dnajc5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings.

GO - Molecular functioni

  • ATP-dependent protein binding Source: RGD
  • glycoprotein binding Source: RGD

GO - Biological processi

  • negative regulation of neuron apoptotic process Source: Ensembl
  • synaptic transmission Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

ReactomeiR-RNO-888590. GABA synthesis, release, reuptake and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 5Curated
Alternative name(s):
Cysteine string protein1 Publication
Short name:
CSP1 Publication
Gene namesi
Name:Dnajc5Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi620516. Dnajc5.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity
  • Melanosome By similarity
  • Cell membrane By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198DnaJ homolog subfamily C member 5PRO_0000071054Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81PhosphoserineCombined sources
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei12 – 121PhosphoserineCombined sources
Modified residuei15 – 151PhosphoserineCombined sources
Modified residuei56 – 561N6-acetyllysineBy similarity
Modified residuei151 – 1511PhosphoserineCombined sources

Post-translational modificationi

Fatty acylated. Heavily palmitoylated in the cysteine string motif.

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP60905.
PRIDEiP60905.

PTM databases

iPTMnetiP60905.
PhosphoSiteiP60905.
SwissPalmiP60905.

Expressioni

Tissue specificityi

Brain. Predominantly associated with nerve endings and synaptic vesicles.

Gene expression databases

ExpressionAtlasiP60905. baseline and differential.
GenevisibleiP60905. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with the chaperone complex consisting of HSC70 and SGTA. Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats) (PubMed:25253725).By similarity1 Publication

GO - Molecular functioni

  • ATP-dependent protein binding Source: RGD
  • glycoprotein binding Source: RGD

Protein-protein interaction databases

BioGridi249414. 1 interaction.
STRINGi10116.ENSRNOP00000020581.

Structurei

3D structure databases

ProteinModelPortaliP60905.
SMRiP60905. Positions 5-100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 8270JPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi118 – 12811Poly-CysAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0716. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000231969.
HOVERGENiHBG005414.
InParanoidiP60905.
KOiK09525.
OMAiFNCCCGR.
OrthoDBiEOG7WHHBD.
PhylomeDBiP60905.
TreeFamiTF105164.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQRQRSLS TSGESLYHVL GLDKNATSDD IKKSYRKLAL KYHPDKNPDN
60 70 80 90 100
PEAADKFKEI NNAHAILTDA TKRNIYDKYG SLGLYVAEQF GEENVNTYFV
110 120 130 140 150
LSSWWAKALF VVCGLLTCCY CCCCLCCCFN CCCGKCKPKA PEGEETEFYV
160 170 180 190
SPEDLEAQLQ SDEREATDTP IVIQPASATE TTQLTADSHP SYHTDGFN
Length:198
Mass (Da):22,101
Last modified:April 13, 2004 - v1
Checksum:i52F98261FBAD978F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39320 mRNA. Translation: AAA81372.1.
S81917 mRNA. Translation: AAB36303.1.
AF323955 mRNA. Translation: AAL04453.1.
PIRiI52655.
RefSeqiNP_077075.1. NM_024161.2.
XP_008760766.1. XM_008762544.1.
XP_008760767.1. XM_008762545.1.
UniGeneiRn.100120.

Genome annotation databases

EnsembliENSRNOT00000020581; ENSRNOP00000020581; ENSRNOG00000015202.
GeneIDi79130.
KEGGirno:79130.
UCSCiRGD:620516. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39320 mRNA. Translation: AAA81372.1.
S81917 mRNA. Translation: AAB36303.1.
AF323955 mRNA. Translation: AAL04453.1.
PIRiI52655.
RefSeqiNP_077075.1. NM_024161.2.
XP_008760766.1. XM_008762544.1.
XP_008760767.1. XM_008762545.1.
UniGeneiRn.100120.

3D structure databases

ProteinModelPortaliP60905.
SMRiP60905. Positions 5-100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249414. 1 interaction.
STRINGi10116.ENSRNOP00000020581.

PTM databases

iPTMnetiP60905.
PhosphoSiteiP60905.
SwissPalmiP60905.

Proteomic databases

PaxDbiP60905.
PRIDEiP60905.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020581; ENSRNOP00000020581; ENSRNOG00000015202.
GeneIDi79130.
KEGGirno:79130.
UCSCiRGD:620516. rat.

Organism-specific databases

CTDi80331.
RGDi620516. Dnajc5.

Phylogenomic databases

eggNOGiKOG0716. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000231969.
HOVERGENiHBG005414.
InParanoidiP60905.
KOiK09525.
OMAiFNCCCGR.
OrthoDBiEOG7WHHBD.
PhylomeDBiP60905.
TreeFamiTF105164.

Enzyme and pathway databases

ReactomeiR-RNO-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

NextBioi614578.
PROiP60905.

Gene expression databases

ExpressionAtlasiP60905. baseline and differential.
GenevisibleiP60905. RN.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide and deduced amino acid sequence of a rat cysteine string protein."
    Mastrogiacomo A., Gundersen C.B.
    Brain Res. Mol. Brain Res. 28:12-18(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Cysteine string protein, a DnaJ family member, is present on diverse secretory vesicles."
    Braun J.E., Scheller R.H.
    Neuropharmacology 34:1361-1369(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Induction of cysteine string protein after chronic antidepressant treatment in rat frontal cortex."
    Yamada M., Yamada M., Yamazaki S., Takahashi K., Nara K., Ozawa H., Yamada S., Kiuchi Y., Oguchi K., Kamijima K., Higuchi T., Momose K.
    Neurosci. Lett. 301:183-186(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Frontal cortex.
  4. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 8-24 AND 42-73, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-10; SER-12; SER-15 AND SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity."
    Lemonidis K., Gorleku O.A., Sanchez-Perez M.C., Grefen C., Chamberlain L.H.
    Mol. Biol. Cell 25:3870-3883(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZDHHC17.

Entry informationi

Entry nameiDNJC5_RAT
AccessioniPrimary (citable) accession number: P60905
Secondary accession number(s): P54101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.