P60904 (DNJC5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily C member 5

Alternative name(s):
Cysteine string protein
Short name=CSP

Gene names

Name:	Dnajc5
Synonyms:	Csp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings By similarity.
Subunit structure	Homodimer Probable. Interacts with the chaperone complex consisting of HSC70 and SGTA. Ref.5
Subcellular location	Membrane; Lipid-anchor. Melanosome By similarity. Cell membrane By similarity. Note: Colocalizes with insulin granules, when overexpressed in a islet cell line. Ref.5
Post-translational modification	Fatty acylated. Heavily palmitoylated in the cysteine string motif. Palmitoylation is not required for membrane association. Ref.5
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Molecular function	Chaperone
PTM	Acetylation Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of neuron apoptotic process Inferred from genetic interaction PubMed 16269331. Source: MGI
Cellular_component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB synaptic vesicle Inferred from direct assay PubMed 16407767. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

DnaJ homolog subfamily C member 5

PRO_0000071053

Regions

Domain

13 – 82

70

J

Compositional bias

118 – 128

11

Poly-Cys

Amino acid modifications

Modified residue

8

1

Phosphoserine Ref.10

Modified residue

10

1

Phosphoserine Ref.4 Ref.7 Ref.8 Ref.9 Ref.11

Modified residue

11

1

Phosphothreonine Ref.6 Ref.7

Modified residue

12

1

Phosphoserine Ref.6 Ref.9

Modified residue

15

1

Phosphoserine Ref.9

Modified residue

17

1

Phosphotyrosine Ref.6

Modified residue

56

1

N6-acetyllysine By similarity

Experimental info

Mutagenesis

113

1

C → V: No change in subcellular location; when associated with G-118 and F-121. Ref.5

Mutagenesis

118

1

C → G: No change in subcellular location; when associated with V-113 and F-121. Ref.5

Mutagenesis

121

1

C → F: No change in subcellular location; when associated with V-113 and G-118. Ref.5

Mutagenesis

129

1

F → C: No change in subcellular location; when associated with H-135. Ref.5

Mutagenesis

135

1

K → H: No change in subcellular location; when associated with C-129. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

198

Helix Strand Turn

Details...

Helix

16 – 20

5

Helix

28 – 41

14

Turn

44 – 46

3

Helix

51 – 67

17

Helix

70 – 78

9

Helix

81 – 89

9

Helix

94 – 100

7

Sequences

	Sequence		Length	Mass (Da)
	P60904 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 52F98261FBAD978F	FASTA	198	22,101
	10 20 30 40 50 60 MADQRQRSLS TSGESLYHVL GLDKNATSDD IKKSYRKLAL KYHPDKNPDN PEAADKFKEI 70 80 90 100 110 120 NNAHAILTDA TKRNIYDKYG SLGLYVAEQF GEENVNTYFV LSSWWAKALF VVCGLLTCCY 130 140 150 160 170 180 CCCCLCCCFN CCCGKCKPKA PEGEETEFYV SPEDLEAQLQ SDEREATDTP IVIQPASATE 190 TTQLTADSHP SYHTDGFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Qin N., Lin T., Birnbaumer L. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Olfactory bulb and Skin.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 8-24 AND 42-72, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY. Tissue: Forebrain.
[5]	"Cysteine-string protein isoform beta (Cspbeta) is targeted to the trans-Golgi network as a non-palmitoylated CSP in clonal beta-cells." Boal F., Le Pevelen S., Cziepluch C., Scotti P., Lang J. Biochim. Biophys. Acta 1773:109-119(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HSC70 AND SGTA, SUBCELLULAR LOCATION, PALMITOYLATION, MUTAGENESIS OF CYS-113; CYS-118; CYS-121; PHE-129 AND LYS-135.
[6]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11; SER-12 AND TYR-17, MASS SPECTROMETRY. Tissue: Teratocarcinoma.
[7]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10 AND THR-11, MASS SPECTROMETRY. Tissue: Brain cortex.
[8]	"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y. Mol. Cell. Proteomics 6:669-676(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-15, MASS SPECTROMETRY. Tissue: Liver.
[10]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, MASS SPECTROMETRY. Tissue: Liver.
[11]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[12]	"Solution structure of J-domain from mouse DnaJ subfamily C member 5." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 5-100.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF032115 mRNA. Translation: AAB87080.1.
AK029006 mRNA. Translation: BAC26236.1.
AK032373 mRNA. Translation: BAC27841.1.

IPI

IPI00132206.

RefSeq

NP_001258513.1. NM_001271584.1.
NP_001258514.1. NM_001271585.1.
NP_058055.1. NM_016775.3.

UniGene

Mm.140761.
Mm.391807.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CTW	NMR	-	A	5-100	[»]

ProteinModelPortal

P60904.

SMR

P60904. Positions 5-100.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000104426.

PTM databases

PhosphoSite

P60904.

Proteomic databases

PaxDb

P60904.

PRIDE

P60904.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000072334; ENSMUSP00000072175; ENSMUSG00000000826.
ENSMUST00000108796; ENSMUSP00000104424; ENSMUSG00000000826.
ENSMUST00000108797; ENSMUSP00000104425; ENSMUSG00000000826.

GeneID

13002.

KEGG

mmu:13002.

Organism-specific databases

CTD

80331.

MGI

MGI:892995. Dnajc5.

Phylogenomic databases

eggNOG

COG0484.

GeneTree

ENSGT00690000102068.

HOGENOM

HOG000231969.

HOVERGEN

HBG005414.

KO

K09525.

OMA

CCCGRCK.

OrthoDB

EOG4NZTVC.

Gene expression databases

ArrayExpress

P60904.

Bgee

P60904.

CleanEx

MM_DNAJC5.

Genevestigator

P60904.

GermOnline

ENSMUSG00000000826. Mus musculus.

Family and domain databases

Gene3D

1.10.287.110. 1 hit.

InterPro

IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]

Pfam

PF00226. DnaJ. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
[Graphical view]

SUPFAM

SSF46565. DnaJ_N. 1 hit.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P60904.

NextBio

282820.

SOURCE

Search...

Entry information

Entry name

DNJC5_MOUSE

Accession

Primary (citable) accession number: P60904
Secondary accession number(s): P54101

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program