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P60904 (DNJC5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DnaJ homolog subfamily C member 5
Alternative name(s):
Cysteine string protein
Short name=CSP
Gene names
Name:Dnajc5
Synonyms:Csp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings By similarity.

Subunit structure

Homodimer Probable. Interacts with the chaperone complex consisting of HSC70 and SGTA. Ref.4

Subcellular location

Membrane; Lipid-anchor. Melanosome By similarity. Cell membrane By similarity. Note: Colocalizes with insulin granules, when overexpressed in a islet cell line. Ref.4

Post-translational modification

Fatty acylated. Heavily palmitoylated in the cysteine string motif. Palmitoylation is not required for membrane association. Ref.4

Sequence similarities

Contains 1 J domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198DnaJ homolog subfamily C member 5
PRO_0000071053

Regions

Domain13 – 8270J
Compositional bias118 – 12811Poly-Cys

Amino acid modifications

Modified residue81Phosphoserine By similarity
Modified residue101Phosphoserine Ref.8
Modified residue151Phosphoserine By similarity
Modified residue561N6-acetyllysine By similarity

Experimental info

Mutagenesis1131C → V: No change in subcellular location; when associated with G-118 and F-121. Ref.4
Mutagenesis1181C → G: No change in subcellular location; when associated with V-113 and F-121. Ref.4
Mutagenesis1211C → F: No change in subcellular location; when associated with V-113 and G-118. Ref.4
Mutagenesis1291F → C: No change in subcellular location; when associated with H-135. Ref.4
Mutagenesis1351K → H: No change in subcellular location; when associated with C-129. Ref.4

Secondary structure

............... 198
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60904 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 52F98261FBAD978F

FASTA19822,101
        10         20         30         40         50         60 
MADQRQRSLS TSGESLYHVL GLDKNATSDD IKKSYRKLAL KYHPDKNPDN PEAADKFKEI 

        70         80         90        100        110        120 
NNAHAILTDA TKRNIYDKYG SLGLYVAEQF GEENVNTYFV LSSWWAKALF VVCGLLTCCY 

       130        140        150        160        170        180 
CCCCLCCCFN CCCGKCKPKA PEGEETEFYV SPEDLEAQLQ SDEREATDTP IVIQPASATE 

       190 
TTQLTADSHP SYHTDGFN 

« Hide

References

« Hide 'large scale' references
[1]Qin N., Lin T., Birnbaumer L.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Olfactory bulb and Skin.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 8-24 AND 42-72, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"Cysteine-string protein isoform beta (Cspbeta) is targeted to the trans-Golgi network as a non-palmitoylated CSP in clonal beta-cells."
Boal F., Le Pevelen S., Cziepluch C., Scotti P., Lang J.
Biochim. Biophys. Acta 1773:109-119(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HSC70 AND SGTA, SUBCELLULAR LOCATION, PALMITOYLATION, MUTAGENESIS OF CYS-113; CYS-118; CYS-121; PHE-129 AND LYS-135.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[9]"Solution structure of J-domain from mouse DnaJ subfamily C member 5."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 5-100.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF032115 mRNA. Translation: AAB87080.1.
AK029006 mRNA. Translation: BAC26236.1.
AK032373 mRNA. Translation: BAC27841.1.
CCDSCCDS17215.1.
RefSeqNP_001258513.1. NM_001271584.1.
NP_001258514.1. NM_001271585.1.
NP_058055.1. NM_016775.3.
UniGeneMm.140761.
Mm.391807.
Mm.491282.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CTWNMR-A5-100[»]
ProteinModelPortalP60904.
SMRP60904. Positions 5-100.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198948. 5 interactions.
IntActP60904. 2 interactions.
MINTMINT-4093239.
STRING10090.ENSMUSP00000104426.

PTM databases

PhosphoSiteP60904.

Proteomic databases

MaxQBP60904.
PaxDbP60904.
PRIDEP60904.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072334; ENSMUSP00000072175; ENSMUSG00000000826.
ENSMUST00000108796; ENSMUSP00000104424; ENSMUSG00000000826.
ENSMUST00000108797; ENSMUSP00000104425; ENSMUSG00000000826.
GeneID13002.
KEGGmmu:13002.
UCSCuc008omp.1. mouse.

Organism-specific databases

CTD80331.
MGIMGI:892995. Dnajc5.

Phylogenomic databases

eggNOGCOG0484.
GeneTreeENSGT00730000110742.
HOGENOMHOG000231969.
HOVERGENHBG005414.
KOK09525.
OMACCCGRCK.
OrthoDBEOG7WHHBD.
PhylomeDBP60904.
TreeFamTF105164.

Gene expression databases

ArrayExpressP60904.
BgeeP60904.
CleanExMM_DNAJC5.
GenevestigatorP60904.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
InterProIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP60904.
NextBio282820.
PROP60904.
SOURCESearch...

Entry information

Entry nameDNJC5_MOUSE
AccessionPrimary (citable) accession number: P60904
Secondary accession number(s): P54101
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot