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Protein

DnaJ homolog subfamily C member 5

Gene

Dnajc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone for the SNARE protein SNAP-25 (PubMed:22187053). Involved in the calcium-mediated control of a late stage of exocytosis (PubMed:20847230). Acts as a general chaperone in regulated exocytosis (By similarity). May have an important role in presynaptic function (By similarity). May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).By similarity2 Publications

GO - Biological processi

  • negative regulation of neuron apoptotic process Source: MGI

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 5Curated
Alternative name(s):
Cysteine string proteinBy similarity
Short name:
CSPBy similarity
Gene namesi
Name:Dnajc5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:892995 Dnajc5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Defective SNAP-25 function, which causes neurodegeneration by impairing SNARE-complex assembly (PubMed:22187053).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10S → D: Reduced interaction with SYT9, but no effect on the interaction with HSC70. 1 Publication1
Mutagenesisi93E → V: Reduced interaction with SYT9. 1 Publication1
Mutagenesisi113C → V: No change in subcellular location; when associated with G-118 and F-121. 1 Publication1
Mutagenesisi118C → G: No change in subcellular location; when associated with V-113 and F-121. 1 Publication1
Mutagenesisi121C → F: No change in subcellular location; when associated with V-113 and G-118. 1 Publication1
Mutagenesisi129F → C: No change in subcellular location; when associated with H-135. 1 Publication1
Mutagenesisi135K → H: No change in subcellular location; when associated with C-129. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710531 – 198DnaJ homolog subfamily C member 5Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei10PhosphoserineCombined sources1
Modified residuei12PhosphoserineBy similarity1
Modified residuei15PhosphoserineCombined sources1
Modified residuei17PhosphotyrosineBy similarity1
Modified residuei56N6-acetyllysineBy similarity1
Modified residuei151PhosphoserineCombined sources1

Post-translational modificationi

Fatty acylated (PubMed:17034881). Heavily palmitoylated in the cysteine string motif (PubMed:17034881). Palmitoylation is not required for membrane association (PubMed:17034881).1 Publication
Formation of the chaperone complex DNAJC5/HSC70 is not regulated by phosphorylation (PubMed:20847230). Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58 (By similarity). This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions (By similarity).By similarity1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiP60904
MaxQBiP60904
PaxDbiP60904
PeptideAtlasiP60904
PRIDEiP60904

PTM databases

iPTMnetiP60904
PhosphoSitePlusiP60904
SwissPalmiP60904

Expressioni

Gene expression databases

BgeeiENSMUSG00000000826
CleanExiMM_DNAJC5
ExpressionAtlasiP60904 baseline and differential
GenevisibleiP60904 MM

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with the chaperone complex consisting of HSC70 and SGTA (PubMed:17034881, PubMed:20847230). Interacts with ZDHHC13 (via ANK repeats) (PubMed:25253725, PubMed:26198635). Interacts with ZDHHC17 (via ANK repeats) (PubMed:25253725, PubMed:26198635). Interacts with SYT1, SYT5 and SYT7, and with SYT9, forming a complex with SNAP25 (PubMed:20847230). The interaction with SYT9 is stimulated tenfold in presence of calcium (PubMed:20847230).Curated4 Publications

Protein-protein interaction databases

BioGridi198948, 5 interactors
CORUMiP60904
IntActiP60904, 2 interactors
MINTiP60904
STRINGi10090.ENSMUSP00000072175

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 20Combined sources5
Helixi28 – 41Combined sources14
Turni44 – 46Combined sources3
Helixi51 – 67Combined sources17
Helixi70 – 78Combined sources9
Helixi81 – 89Combined sources9
Helixi94 – 100Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CTWNMR-A5-100[»]
ProteinModelPortaliP60904
SMRiP60904
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60904

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 82JPROSITE-ProRule annotationAdd BLAST70

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi118 – 128Poly-CysAdd BLAST11

Phylogenomic databases

eggNOGiKOG0716 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00910000144120
HOGENOMiHOG000231969
HOVERGENiHBG005414
InParanoidiP60904
KOiK09525
OMAiGHYSYHT
OrthoDBiEOG091G0QJP
PhylomeDBiP60904
TreeFamiTF105164

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P60904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQRQRSLS TSGESLYHVL GLDKNATSDD IKKSYRKLAL KYHPDKNPDN
60 70 80 90 100
PEAADKFKEI NNAHAILTDA TKRNIYDKYG SLGLYVAEQF GEENVNTYFV
110 120 130 140 150
LSSWWAKALF VVCGLLTCCY CCCCLCCCFN CCCGKCKPKA PEGEETEFYV
160 170 180 190
SPEDLEAQLQ SDEREATDTP IVIQPASATE TTQLTADSHP SYHTDGFN
Length:198
Mass (Da):22,101
Last modified:April 13, 2004 - v1
Checksum:i52F98261FBAD978F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF032115 mRNA Translation: AAB87080.1
AK029006 mRNA Translation: BAC26236.1
AK032373 mRNA Translation: BAC27841.1
CCDSiCCDS17215.1
RefSeqiNP_001258513.1, NM_001271584.1
NP_001258514.1, NM_001271585.1
NP_058055.1, NM_016775.3
XP_011238225.1, XM_011239923.2
UniGeneiMm.140761
Mm.391807
Mm.491282

Genome annotation databases

EnsembliENSMUST00000072334; ENSMUSP00000072175; ENSMUSG00000000826
ENSMUST00000108796; ENSMUSP00000104424; ENSMUSG00000000826
ENSMUST00000108797; ENSMUSP00000104425; ENSMUSG00000000826
GeneIDi13002
KEGGimmu:13002
UCSCiuc008omp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDNJC5_MOUSE
AccessioniPrimary (citable) accession number: P60904
Secondary accession number(s): P54101
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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