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P60901

- PSA6_RAT

UniProt

P60901 - PSA6_RAT

Protein

Proteasome subunit alpha type-6

Gene

Psma6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. RNA binding Source: BHF-UCL
    2. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
    2. proteolysis involved in cellular protein catabolic process Source: BHF-UCL
    3. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_198391. Asymmetric localization of PCP proteins.
    REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199197. ER-Phagosome pathway.
    REACT_199247. Activation of NF-kappaB in B cells.
    REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206488. degradation of DVL.
    REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
    REACT_211117. Orc1 removal from chromatin.
    REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_220232. Regulation of ornithine decarboxylase (ODC).
    REACT_227706. degradation of AXIN.

    Protein family/group databases

    MEROPSiT01.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha type-6 (EC:3.4.25.1)
    Alternative name(s):
    Macropain iota chain
    Multicatalytic endopeptidase complex iota chain
    Proteasome iota chain
    Gene namesi
    Name:Psma6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi61849. Psma6.

    Subcellular locationi

    Cytoplasm. Nucleus. CytoplasmP-body By similarity
    Note: Colocalizes with TRIM5 in the cytoplasmic bodies.By similarity

    GO - Cellular componenti

    1. cytoplasmic mRNA processing body Source: UniProtKB
    2. mitochondrion Source: Ensembl
    3. myofibril Source: BHF-UCL
    4. nuclear matrix Source: BHF-UCL
    5. nucleus Source: BHF-UCL
    6. polysome Source: BHF-UCL
    7. proteasome core complex Source: UniProtKB
    8. proteasome core complex, alpha-subunit complex Source: BHF-UCL
    9. sarcomere Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246Proteasome subunit alpha type-6PRO_0000124132Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi5 – 51O-linked (GlcNAc)By similarity
    Cross-linki59 – 59Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei102 – 1021N6-acetyllysineBy similarity
    Modified residuei104 – 1041N6-acetyllysineBy similarity
    Modified residuei159 – 1591PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP60901.
    PRIDEiP60901.

    2D gel databases

    World-2DPAGE0004:P60901.

    PTM databases

    PhosphoSiteiP60901.

    Expressioni

    Gene expression databases

    GenevestigatoriP60901.

    Interactioni

    Subunit structurei

    Interacts with ALKBH4 By similarity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.By similarity

    Protein-protein interaction databases

    BioGridi248294. 2 interactions.
    IntActiP60901. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP60901.
    SMRiP60901. Positions 2-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00550000074807.
    HOGENOMiHOG000091084.
    HOVERGENiHBG107363.
    InParanoidiP60901.
    KOiK02730.
    OMAiMSRTSYD.
    OrthoDBiEOG70PBZH.
    PhylomeDBiP60901.
    TreeFamiTF106210.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60901-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRGSSAGFD RHITIFSPEG RLYQVEYAFK AINQGGLTSV AVRGKDCAVI    50
    VTQKKVPDKL LDSSTVTHLF KITENIGCVM TGMTADSRSQ VQRARYEAAN 100
    WKYKYGYEIP VDMLCKRIAD ISQVYTQNAE MRPLGCCMIL IGIDEEQGPQ 150
    VYKCDPAGYY CGFKATAAGV KQTESTSFLE KKVKKKFDWT FEQTVETAIT 200
    CLSTVLSIDF KPSEIEVGVV TVENPKFRIL TEAEIDAHLV ALAERD 246
    Length:246
    Mass (Da):27,399
    Last modified:April 13, 2004 - v1
    Checksum:i94D1FD3C0A7CC72A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10755 mRNA. Translation: BAA01587.1.
    BC062232 mRNA. Translation: AAH62232.1.
    PIRiJX0230.
    RefSeqiNP_058979.1. NM_017283.3.
    UniGeneiRn.107278.

    Genome annotation databases

    EnsembliENSRNOT00000009666; ENSRNOP00000009666; ENSRNOG00000007114.
    GeneIDi29673.
    KEGGirno:29673.
    UCSCiRGD:61849. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10755 mRNA. Translation: BAA01587.1 .
    BC062232 mRNA. Translation: AAH62232.1 .
    PIRi JX0230.
    RefSeqi NP_058979.1. NM_017283.3.
    UniGenei Rn.107278.

    3D structure databases

    ProteinModelPortali P60901.
    SMRi P60901. Positions 2-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248294. 2 interactions.
    IntActi P60901. 1 interaction.

    Protein family/group databases

    MEROPSi T01.971.

    PTM databases

    PhosphoSitei P60901.

    2D gel databases

    World-2DPAGE 0004:P60901.

    Proteomic databases

    PaxDbi P60901.
    PRIDEi P60901.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000009666 ; ENSRNOP00000009666 ; ENSRNOG00000007114 .
    GeneIDi 29673.
    KEGGi rno:29673.
    UCSCi RGD:61849. rat.

    Organism-specific databases

    CTDi 5687.
    RGDi 61849. Psma6.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00550000074807.
    HOGENOMi HOG000091084.
    HOVERGENi HBG107363.
    InParanoidi P60901.
    KOi K02730.
    OMAi MSRTSYD.
    OrthoDBi EOG70PBZH.
    PhylomeDBi P60901.
    TreeFami TF106210.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_198391. Asymmetric localization of PCP proteins.
    REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199197. ER-Phagosome pathway.
    REACT_199247. Activation of NF-kappaB in B cells.
    REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206488. degradation of DVL.
    REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
    REACT_211117. Orc1 removal from chromatin.
    REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_220232. Regulation of ornithine decarboxylase (ODC).
    REACT_227706. degradation of AXIN.

    Miscellaneous databases

    NextBioi 22090.
    PROi P60901.

    Gene expression databases

    Genevestigatori P60901.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNAs for rat proteasomes: deduced primary structures of four other subunits."
      Tamura T., Shimbara N., Aki M., Ishida N., Bey F., Scherrer K., Tanaka K., Ichihara A.
      J. Biochem. 112:530-534(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    3. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 12-21; 60-71; 154-164 AND 229-246, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiPSA6_RAT
    AccessioniPrimary (citable) accession number: P60901
    Secondary accession number(s): P34062
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3