P60893 (GPR85_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable G-protein coupled receptor 85

Alternative name(s):
Super conserved receptor expressed in brain 2

Gene names

Name:	GPR85
Synonyms:	SREB2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Orphan receptor.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Highly expressed in brain and testis. Lower levels in small intestine, placenta and spleen. In brain regions, detected in all regions tested, but somewhat lower levels in the corpus callosum, medulla and spinal cord. Ref.1 Ref.2
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: LIFEdb
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Probable G-protein coupled receptor 85		PRO_0000069591
Regions
	Topological domain	1 – 25	25	Extracellular Potential
	Transmembrane	26 – 46	21	Helical; Name=1; Potential
	Topological domain	47 – 57	11	Cytoplasmic Potential
	Transmembrane	58 – 78	21	Helical; Name=2; Potential
	Topological domain	79 – 96	18	Extracellular Potential
	Transmembrane	97 – 117	21	Helical; Name=3; Potential
	Topological domain	118 – 137	20	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Name=4; Potential
	Topological domain	159 – 188	30	Extracellular Potential
	Transmembrane	189 – 209	21	Helical; Name=5; Potential
	Topological domain	210 – 286	77	Cytoplasmic Potential
	Transmembrane	287 – 307	21	Helical; Name=6; Potential
	Topological domain	308 – 313	6	Extracellular Potential
	Transmembrane	314 – 334	21	Helical; Name=7; Potential
	Topological domain	335 – 370	36	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 172		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60893 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 7B67A39F6166AAEB	FASTA	370	41,995
	10 20 30 40 50 60 MANYSHAADN ILQNLSPLTA FLKLTSLGFI IGVSVVGNLL ISILLVKDKT LHRAPYYFLL 70 80 90 100 110 120 DLCCSDILRS AICFPFVFNS VKNGSTWTYG TLTCKVIAFL GVLSCFHTAF MLFCISVTRY 130 140 150 160 170 180 LAIAHHRFYT KRLTFWTCLA VICMVWTLSV AMAFPPVLDV GTYSFIREED QCTFQHRSFR 190 200 210 220 230 240 ANDSLGFMLL LALILLATQL VYLKLIFFVH DRRKMKPVQF VAAVSQNWTF HGPGASGQAA 250 260 270 280 290 300 ANWLAGFGRG PTPPTLLGIR QNANTTGRRR LLVLDEFKME KRISRMFYIM TFLFLTLWGP 310 320 330 340 350 360 YLVACYWRVF ARGPVVPGGF LTAAVWMSFA QAGINPFVCI FSNRELRRCF STTLLYCRKS 370 RLPREPYCVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The brain-specific G-protein coupled receptor GPR85 with identical protein sequence in man and mouse maps to human chromosome 7q31." Hellebrand S., Schaller H.C., Wittenberger T. Biochim. Biophys. Acta 1493:269-272(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[2]	"An evolutionarily conserved G-protein coupled receptor family, SREB, expressed in the central nervous system." Matsumoto M., Saito T., Takasaki J., Kamohara M., Sugimoto T., Kobayashi M., Tadokoro M., Matsumoto S., Ohishi T., Furuichi K. Biochem. Biophys. Res. Commun. 272:576-582(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[3]	"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[5]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF250237 mRNA. Translation: AAF79956.1. AB040800 mRNA. Translation: BAA96646.1. AB065688 Genomic DNA. Translation: BAC05911.1. AL161959 mRNA. Translation: CAB82307.1. AC073346 Genomic DNA. Translation: AAQ93365.1.
IPI	IPI00014802.
PIR	T47131.
RefSeq	NP_001139737.1. NM_001146265.1. NP_001139738.1. NM_001146266.1. NP_001139739.1. NM_001146267.1. NP_061843.3. NM_018970.6.
UniGene	Hs.152009.
3D structure databases
ProteinModelPortal	P60893.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4724443.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P60893.
Polymorphism databases
DMDM	46397442.
Proteomic databases
PaxDb	P60893.
PRIDE	P60893.
Protocols and materials databases
DNASU	54329.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000297146; ENSP00000297146; ENSG00000164604. ENST00000424100; ENSP00000396763; ENSG00000164604. ENST00000449591; ENSP00000401178; ENSG00000164604. ENST00000501255; ENSP00000445808; ENSG00000164604.
GeneID	54329.
KEGG	hsa:54329.
UCSC	uc003vgp.1. human.
Organism-specific databases
CTD	54329.
GeneCards	GC07M112720.
HGNC	HGNC:4536. GPR85.
MIM	605188. gene.
neXtProt	NX_P60893.
PharmGKB	PA28929.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG237432.
HOGENOM	HOG000231471.
HOVERGEN	HBG051805.
InParanoid	P60893.
KO	K04302.
OMA	SMANYSH.
OrthoDB	EOG4HQDJK.
PhylomeDB	P60893.
Gene expression databases
ArrayExpress	P60893.
Bgee	P60893.
CleanEx	HS_GPR85.
Genevestigator	P60893.
GermOnline	ENSG00000164604. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	54329.
NextBio	56567.
SOURCE	Search...

Entry information

Entry name

GPR85_HUMAN

Accession

Primary (citable) accession number: P60893
Secondary accession number(s): Q9JHI6, Q9NPD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.