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P60892

- PRPS1_RAT

UniProt

P60892 - PRPS1_RAT

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Protein
Ribose-phosphate pyrophosphokinase 1
Gene
Prps1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.UniRule annotation

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Magnesium.

Enzyme regulationi

Activated by magnesium and inorganic phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi128 – 1281Magnesium Reviewed prediction
Metal bindingi130 – 1301Magnesium Reviewed prediction
Binding sitei130 – 1301ATP By similarity
Metal bindingi139 – 1391Magnesium Reviewed prediction
Metal bindingi143 – 1431Magnesium Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi96 – 1016ATP By similarity

GO - Molecular functioni

  1. ADP binding Source: RGD
  2. AMP binding Source: RGD
  3. ATP binding Source: UniProtKB
  4. GDP binding Source: RGD
  5. carbohydrate binding Source: RGD
  6. kinase activity Source: UniProtKB-KW
  7. magnesium ion binding Source: RGD
  8. protein binding Source: RGD
  9. protein homodimerization activity Source: UniProtKB
  10. ribose phosphate diphosphokinase activity Source: UniProtKB

GO - Biological processi

  1. 5-phosphoribose 1-diphosphate biosynthetic process Source: RGD
  2. AMP biosynthetic process Source: RGD
  3. hypoxanthine biosynthetic process Source: Ensembl
  4. nervous system development Source: Ensembl
  5. nucleotide biosynthetic process Source: RGD
  6. organ regeneration Source: RGD
  7. ribose phosphate metabolic process Source: RGD
  8. urate biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_209102. 5-Phosphoribose 1-diphosphate biosynthesis.
SABIO-RKP60892.
UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 1 (EC:2.7.6.1)
Alternative name(s):
Phosphoribosyl pyrophosphate synthase I
Short name:
PRS-I
Gene namesi
Name:Prps1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi61955. Prps1.

Subcellular locationi

GO - Cellular componenti

  1. protein complex Source: RGD
  2. ribose phosphate diphosphokinase complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 318317Ribose-phosphate pyrophosphokinase 1UniRule annotation
PRO_0000141073Add
BLAST

Proteomic databases

PaxDbiP60892.
PRIDEiP60892.

2D gel databases

World-2DPAGE0004:P60892.

PTM databases

PhosphoSiteiP60892.

Expressioni

Gene expression databases

GenevestigatoriP60892.

Interactioni

Subunit structurei

Homodimer. The active form is probably a hexamer composed of 3 homodimers By similarity.

Protein-protein interaction databases

BioGridi248196. 2 interactions.
IntActiP60892. 1 interaction.
STRINGi10116.ENSRNOP00000029405.

Structurei

3D structure databases

ProteinModelPortaliP60892.
SMRiP60892. Positions 3-317.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 22716Binding of phosphoribosylpyrophosphate Reviewed prediction
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0462.
GeneTreeiENSGT00550000074583.
HOGENOMiHOG000210451.
HOVERGENiHBG001520.
InParanoidiP60892.
KOiK00948.
OMAiHIPENIE.
OrthoDBiEOG7G4QG5.
PhylomeDBiP60892.
TreeFamiTF106366.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_B. RibP_PPkinase_B.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60892-1 [UniParc]FASTAAdd to Basket

« Hide

MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG    50
EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK 100
DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA 150
VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN 200
EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH 250
GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI 300
RRTHNGESVS YLFSHVPL 318
Length:318
Mass (Da):34,834
Last modified:January 23, 2007 - v2
Checksum:i46D017E969908BA0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti163 – 1631R → K in AAH88149. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17258 mRNA. Translation: AAA41963.1.
M29392 mRNA. Translation: AAA41960.1.
X16554 mRNA. Translation: CAA34555.1.
M31084
, M31078, M31079, M31080, M31082, M31083 Genomic DNA. Translation: AAA41959.1.
BC078853 mRNA. Translation: AAH78853.1.
BC088149 mRNA. Translation: AAH88149.1.
PIRiA35465. KIRTR1.
RefSeqiNP_001009694.1. NM_001009694.1.
NP_058939.1. NM_017243.2.
UniGeneiRn.91470.
Rn.9761.

Genome annotation databases

EnsembliENSRNOT00000029571; ENSRNOP00000029405; ENSRNOG00000025166.
ENSRNOT00000040360; ENSRNOP00000043734; ENSRNOG00000031938.
GeneIDi29562.
314140.
KEGGirno:29562.
rno:314140.
UCSCiRGD:61955. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17258 mRNA. Translation: AAA41963.1 .
M29392 mRNA. Translation: AAA41960.1 .
X16554 mRNA. Translation: CAA34555.1 .
M31084
, M31078 , M31079 , M31080 , M31082 , M31083 Genomic DNA. Translation: AAA41959.1 .
BC078853 mRNA. Translation: AAH78853.1 .
BC088149 mRNA. Translation: AAH88149.1 .
PIRi A35465. KIRTR1.
RefSeqi NP_001009694.1. NM_001009694.1.
NP_058939.1. NM_017243.2.
UniGenei Rn.91470.
Rn.9761.

3D structure databases

ProteinModelPortali P60892.
SMRi P60892. Positions 3-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248196. 2 interactions.
IntActi P60892. 1 interaction.
STRINGi 10116.ENSRNOP00000029405.

Chemistry

BindingDBi P60892.

PTM databases

PhosphoSitei P60892.

2D gel databases

World-2DPAGE 0004:P60892.

Proteomic databases

PaxDbi P60892.
PRIDEi P60892.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000029571 ; ENSRNOP00000029405 ; ENSRNOG00000025166 .
ENSRNOT00000040360 ; ENSRNOP00000043734 ; ENSRNOG00000031938 .
GeneIDi 29562.
314140.
KEGGi rno:29562.
rno:314140.
UCSCi RGD:61955. rat.

Organism-specific databases

CTDi 5631.
RGDi 61955. Prps1.

Phylogenomic databases

eggNOGi COG0462.
GeneTreei ENSGT00550000074583.
HOGENOMi HOG000210451.
HOVERGENi HBG001520.
InParanoidi P60892.
KOi K00948.
OMAi HIPENIE.
OrthoDBi EOG7G4QG5.
PhylomeDBi P60892.
TreeFami TF106366.

Enzyme and pathway databases

UniPathwayi UPA00087 ; UER00172 .
Reactomei REACT_209102. 5-Phosphoribose 1-diphosphate biosynthesis.
SABIO-RK P60892.

Miscellaneous databases

NextBioi 21886.
PROi P60892.

Gene expression databases

Genevestigatori P60892.

Family and domain databases

Gene3Di 3.40.50.2020. 2 hits.
HAMAPi MF_00583_B. RibP_PPkinase_B.
InterProi IPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view ]
Pfami PF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01251. ribP_PPkin. 1 hit.
PROSITEi PS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide and deduced amino acid sequences of two distinct cDNAs for rat phosphoribosylpyrophosphate synthetase."
    Taira M., Ishijima S., Kita K., Yamada K., Iizasa T., Tatibana M.
    J. Biol. Chem. 262:14867-14870(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Complete cDNA sequence of rat phosphoribosylpyrophosphate synthetase subunit I (PRS I)."
    Ishijima S., Taira M., Tatibana M.
    Nucleic Acids Res. 17:8860-8860(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure of the rat PRPS1 gene encoding phosphoribosylpyrophosphate synthetase subunit I."
    Shimada H., Taira M., Yamada K., Iizasa T., Tatibana M.
    J. Biol. Chem. 265:3956-3960(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart and Thymus.
  5. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 85-96, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.

Entry informationi

Entry nameiPRPS1_RAT
AccessioniPrimary (citable) accession number: P60892
Secondary accession number(s): P09329, Q5M8A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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