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P60892

- PRPS1_RAT

UniProt

P60892 - PRPS1_RAT

Protein

Ribose-phosphate pyrophosphokinase 1

Gene

Prps1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

    Catalytic activityi

    ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activated by magnesium and inorganic phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi128 – 1281MagnesiumSequence Analysis
    Metal bindingi130 – 1301MagnesiumSequence Analysis
    Binding sitei130 – 1301ATPBy similarity
    Metal bindingi139 – 1391MagnesiumSequence Analysis
    Metal bindingi143 – 1431MagnesiumSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi96 – 1016ATPBy similarity

    GO - Molecular functioni

    1. ADP binding Source: RGD
    2. AMP binding Source: RGD
    3. ATP binding Source: UniProtKB
    4. carbohydrate binding Source: RGD
    5. GDP binding Source: RGD
    6. kinase activity Source: UniProtKB-KW
    7. magnesium ion binding Source: RGD
    8. protein binding Source: RGD
    9. protein homodimerization activity Source: UniProtKB
    10. ribose phosphate diphosphokinase activity Source: UniProtKB

    GO - Biological processi

    1. 5-phosphoribose 1-diphosphate biosynthetic process Source: RGD
    2. AMP biosynthetic process Source: RGD
    3. hypoxanthine biosynthetic process Source: Ensembl
    4. nervous system development Source: Ensembl
    5. nucleotide biosynthetic process Source: RGD
    6. organ regeneration Source: RGD
    7. ribose phosphate metabolic process Source: RGD
    8. urate biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_209102. 5-Phosphoribose 1-diphosphate biosynthesis.
    SABIO-RKP60892.
    UniPathwayiUPA00087; UER00172.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribose-phosphate pyrophosphokinase 1 (EC:2.7.6.1)
    Alternative name(s):
    Phosphoribosyl pyrophosphate synthase I
    Short name:
    PRS-I
    Gene namesi
    Name:Prps1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi61955. Prps1.

    Subcellular locationi

    GO - Cellular componenti

    1. protein complex Source: RGD
    2. ribose phosphate diphosphokinase complex Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 318317Ribose-phosphate pyrophosphokinase 1PRO_0000141073Add
    BLAST

    Proteomic databases

    PaxDbiP60892.
    PRIDEiP60892.

    2D gel databases

    World-2DPAGE0004:P60892.

    PTM databases

    PhosphoSiteiP60892.

    Expressioni

    Gene expression databases

    GenevestigatoriP60892.

    Interactioni

    Subunit structurei

    Homodimer. The active form is probably a hexamer composed of 3 homodimers By similarity.By similarity

    Protein-protein interaction databases

    BioGridi248196. 2 interactions.
    IntActiP60892. 1 interaction.
    STRINGi10116.ENSRNOP00000029405.

    Structurei

    3D structure databases

    ProteinModelPortaliP60892.
    SMRiP60892. Positions 3-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 22716Binding of phosphoribosylpyrophosphateSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0462.
    GeneTreeiENSGT00550000074583.
    HOGENOMiHOG000210451.
    HOVERGENiHBG001520.
    InParanoidiP60892.
    KOiK00948.
    OMAiHIPENIE.
    OrthoDBiEOG7G4QG5.
    PhylomeDBiP60892.
    TreeFamiTF106366.

    Family and domain databases

    Gene3Di3.40.50.2020. 2 hits.
    HAMAPiMF_00583_B. RibP_PPkinase_B.
    InterProiIPR000842. PRib_PP_synth_CS.
    IPR029099. Pribosyltran_N.
    IPR029057. PRTase-like.
    IPR005946. Rib-P_diPkinase.
    [Graphical view]
    PfamiPF14572. Pribosyl_synth. 1 hit.
    PF13793. Pribosyltran_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
    PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P60892-1 [UniParc]FASTAAdd to Basket

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    MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG    50
    EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK 100
    DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA 150
    VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN 200
    EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH 250
    GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI 300
    RRTHNGESVS YLFSHVPL 318
    Length:318
    Mass (Da):34,834
    Last modified:January 23, 2007 - v2
    Checksum:i46D017E969908BA0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti163 – 1631R → K in AAH88149. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17258 mRNA. Translation: AAA41963.1.
    M29392 mRNA. Translation: AAA41960.1.
    X16554 mRNA. Translation: CAA34555.1.
    M31084
    , M31078, M31079, M31080, M31082, M31083 Genomic DNA. Translation: AAA41959.1.
    BC078853 mRNA. Translation: AAH78853.1.
    BC088149 mRNA. Translation: AAH88149.1.
    PIRiA35465. KIRTR1.
    RefSeqiNP_001009694.1. NM_001009694.1.
    NP_058939.1. NM_017243.2.
    UniGeneiRn.91470.
    Rn.9761.

    Genome annotation databases

    EnsembliENSRNOT00000029571; ENSRNOP00000029405; ENSRNOG00000025166.
    ENSRNOT00000040360; ENSRNOP00000043734; ENSRNOG00000031938.
    GeneIDi29562.
    314140.
    KEGGirno:29562.
    rno:314140.
    UCSCiRGD:61955. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17258 mRNA. Translation: AAA41963.1 .
    M29392 mRNA. Translation: AAA41960.1 .
    X16554 mRNA. Translation: CAA34555.1 .
    M31084
    , M31078 , M31079 , M31080 , M31082 , M31083 Genomic DNA. Translation: AAA41959.1 .
    BC078853 mRNA. Translation: AAH78853.1 .
    BC088149 mRNA. Translation: AAH88149.1 .
    PIRi A35465. KIRTR1.
    RefSeqi NP_001009694.1. NM_001009694.1.
    NP_058939.1. NM_017243.2.
    UniGenei Rn.91470.
    Rn.9761.

    3D structure databases

    ProteinModelPortali P60892.
    SMRi P60892. Positions 3-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248196. 2 interactions.
    IntActi P60892. 1 interaction.
    STRINGi 10116.ENSRNOP00000029405.

    Chemistry

    BindingDBi P60892.

    PTM databases

    PhosphoSitei P60892.

    2D gel databases

    World-2DPAGE 0004:P60892.

    Proteomic databases

    PaxDbi P60892.
    PRIDEi P60892.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000029571 ; ENSRNOP00000029405 ; ENSRNOG00000025166 .
    ENSRNOT00000040360 ; ENSRNOP00000043734 ; ENSRNOG00000031938 .
    GeneIDi 29562.
    314140.
    KEGGi rno:29562.
    rno:314140.
    UCSCi RGD:61955. rat.

    Organism-specific databases

    CTDi 5631.
    RGDi 61955. Prps1.

    Phylogenomic databases

    eggNOGi COG0462.
    GeneTreei ENSGT00550000074583.
    HOGENOMi HOG000210451.
    HOVERGENi HBG001520.
    InParanoidi P60892.
    KOi K00948.
    OMAi HIPENIE.
    OrthoDBi EOG7G4QG5.
    PhylomeDBi P60892.
    TreeFami TF106366.

    Enzyme and pathway databases

    UniPathwayi UPA00087 ; UER00172 .
    Reactomei REACT_209102. 5-Phosphoribose 1-diphosphate biosynthesis.
    SABIO-RK P60892.

    Miscellaneous databases

    NextBioi 21886.
    PROi P60892.

    Gene expression databases

    Genevestigatori P60892.

    Family and domain databases

    Gene3Di 3.40.50.2020. 2 hits.
    HAMAPi MF_00583_B. RibP_PPkinase_B.
    InterProi IPR000842. PRib_PP_synth_CS.
    IPR029099. Pribosyltran_N.
    IPR029057. PRTase-like.
    IPR005946. Rib-P_diPkinase.
    [Graphical view ]
    Pfami PF14572. Pribosyl_synth. 1 hit.
    PF13793. Pribosyltran_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01251. ribP_PPkin. 1 hit.
    PROSITEi PS00114. PRPP_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide and deduced amino acid sequences of two distinct cDNAs for rat phosphoribosylpyrophosphate synthetase."
      Taira M., Ishijima S., Kita K., Yamada K., Iizasa T., Tatibana M.
      J. Biol. Chem. 262:14867-14870(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Complete cDNA sequence of rat phosphoribosylpyrophosphate synthetase subunit I (PRS I)."
      Ishijima S., Taira M., Tatibana M.
      Nucleic Acids Res. 17:8860-8860(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Structure of the rat PRPS1 gene encoding phosphoribosylpyrophosphate synthetase subunit I."
      Shimada H., Taira M., Yamada K., Iizasa T., Tatibana M.
      J. Biol. Chem. 265:3956-3960(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart and Thymus.
    5. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 85-96, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiPRPS1_RAT
    AccessioniPrimary (citable) accession number: P60892
    Secondary accession number(s): P09329, Q5M8A4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3