Reviewed, UniProtKB/Swiss-Prot P60881 (SNP25_RAT)

Names and origin

Protein names

Recommended name:
    Synaptosomal-associated protein 25
      Short name=SNAP-25
Alternative name(s):
    Synaptosomal-associated 25 kDa protein
    Super protein
      Short name=SUP

Gene names

Name:	Snap25
Synonyms:	Snap

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Subunit structure	Interacts with CENP and OTOF. Found in a complex containing SYT1, SV2B and syntaxin-1 By similarity. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1, SNAP25BP, and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8.
Subcellular location	Cytoplasm › perinuclear region By similarity. Cell membrane; Lipid-anchor. Cell junction › synapse › synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region By similarity.
Post-translational modification	Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association By similarity.
Sequence similarities	Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Synapse Synaptosome
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	axonogenesis Inferred from expression pattern. Source: RGD calcium ion-dependent exocytosis of neurotransmitter Inferred from direct assay. Source: RGD endosome transport Inferred from direct assay. Source: RGD exocytosis Traceable author statement. Source: RGD growth hormone secretion Inferred from expression pattern. Source: RGD long-term memory Inferred from mutant phenotype. Source: RGD positive regulation of insulin secretion Inferred from expression pattern. Source: RGD regulation of synaptogenesis Inferred from expression pattern. Source: RGD sleep Inferred from direct assay. Source: RGD
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from direct assay. Source: RGD growth cone Inferred from direct assay. Source: HGNC membrane raft Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptobrevin 2-SNAP-25-syntaxin-1a complex Inferred from direct assay. Source: MGI synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex Inferred from direct assay. Source: MGI synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell voltage-gated potassium channel complex Inferred from physical interaction. Source: RGD
Molecular function	SNAP receptor activity Traceable author statement. Source: RGD myosin binding Inferred from physical interaction. Source: RGD protein N-terminus binding Inferred from physical interaction. Source: RGD syntaxin-1 binding Inferred from direct assay. Source: MGI voltage-gated potassium channel activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
P140	Q9QXY2	1	EBI-1027214,EBI-1394088

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoforms differ by the usage of two alternative homologous exons (5a and 5b) which encode for positions 56 to 94 and differ only in 9 positions out of 39.

Isoform SNAP-25b (identifier: P60881-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform SNAP-25a (identifier: P60881-2)

The sequence of this isoform differs from the canonical sequence as follows:
     58-89: ERIEEGMDQINKDMKEAEKNLTDLGKFCGLCV → DRVEEGMNHINQDMKEAEKNLKDLGKCCGLFI

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 206

206

Synaptosomal-associated protein 25

PRO_0000213592

Regions

Domain

19 – 81

63

t-SNARE coiled-coil homology 1

Domain

140 – 202

63

t-SNARE coiled-coil homology 2

Region

1 – 75

75

Interaction with CENPF By similarity

Compositional bias

85 – 92

8

Cys-rich

Sites

Site

180 – 181

2

Cleavage; by BONT/E By similarity

Amino acid modifications

Modified residue

138

1

Phosphothreonine; by PKC and PKA Ref.7

Modified residue

187

1

Phosphoserine; by PKC Ref.7

Lipidation

85

1

S-palmitoyl cysteine By similarity

Lipidation

88

1

S-palmitoyl cysteine By similarity

Lipidation

90

1

S-palmitoyl cysteine By similarity

Lipidation

92

1

S-palmitoyl cysteine By similarity

Natural variations

Alternative sequence

58 – 89

32

ERIEE…CGLCV → DRVEEGMNHINQDMKEAEKN LKDLGKCCGLFI in isoform SNAP-25a.

VSP_010020

Secondary structure

1

.

.

.

.

.

206

Helix Strand Turn

Details...

Helix

7 – 82

76

Helix

142 – 201

60

Sequences

	Sequence		Length	Mass (Da)
	Isoform SNAP-25b [UniParc]. Last modified April 13, 2004. Version 1. Checksum: FBED2B082A4CB6A6	FASTA	206	23,315
	10 20 30 40 50 60 MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI 70 80 90 100 110 120 EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV 130 140 150 160 170 180 VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR 190 200 IMEKADSNKT RIDEANQRAT KMLGSG
	Isoform SNAP-25a [UniParc] [UniParc]. Checksum: E272652C701EA984	FASTA	206	23,336
	10 20 30 40 50 60 MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLDRV 70 80 90 100 110 120 EEGMNHINQD MKEAEKNLKD LGKCCGLFIC PCNKLKSSDA YKKAWGNNQD GVVASQPARV 130 140 150 160 170 180 VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR 190 200 IMEKADSNKT RIDEANQRAT KMLGSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kataoka M. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SNAP-25A AND SNAP-25B).
[2]	"Cloning of the SNAP-25 gene from a rat brain cDNA library." Cho A.R., You K.H. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SNAP-25A). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SNAP-25B). Tissue: Brain.
[4]	"SNARE complex proteins, including the cognate pair VAMP-2 and syntaxin-4, are expressed in cultured oligodendrocytes." Madison D.L., Krueger W.H., Cheng D., Trapp B.D., Pfeiffer S.E. J. Neurochem. 72:988-998(1999) [PubMed: 10037470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-190 (ISOFORM SNAP-25B). Tissue: Brain.
[5]	Lubec G., Afjehi-Sadat L., Diao W., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 46-69; 84-94; 104-119; 125-136 AND 143-161, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[6]	"The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS." Hess D.T., Slater T.M., Wilson M.C., Skene J.H.P. J. Neurosci. 12:4634-4641(1992) [PubMed: 1281490] [Abstract] Cited for: PALMITOYLATION, SUBCELLULAR LOCATION.
[7]	"Differential phosphorylation of SNAP-25 in vivo by protein kinase C and protein kinase A." Hepp R., Cabaniols J.-P., Roche P.A. FEBS Lett. 532:52-56(2002) [PubMed: 12459461] [Abstract] Cited for: PHOSPHORYLATION AT THR-138 AND SER-187.
[8]	"Differential subcellular localization of SNAP-25a and SNAP-25b RNA transcripts in spinal motoneurons and plasticity in expression after nerve injury." Jacobsson G., Piehl F., Bark I.C., Zhang X., Meister B. Brain Res. Mol. Brain Res. 37:49-62(1996) [PubMed: 8738135] [Abstract] Cited for: SUBCELLULAR LOCATION OF RNA TRANSCRIPTS.
[9]	"Hrs-2 is an ATPase implicated in calcium-regulated secretion." Bean A.J., Seifert R., Chen Y.A., Sacks R., Scheller R.H. Nature 385:826-829(1997) [PubMed: 9039916] [Abstract] Cited for: INTERACTION WITH HGS.
[10]	"Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties." Fasshauer D., Antonin W., Margittai M., Pabst S., Jahn R. J. Biol. Chem. 274:15440-15446(1999) [PubMed: 10336434] [Abstract] Cited for: IDENTIFICATION IN A TERNARY COMPLEX WITH STX1A AND VAMP8.
[11]	"Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin." Coppola T., Magnin-Luethi S., Perret-Menoud V., Gattesco S., Schiavo G., Regazzi R. J. Biol. Chem. 276:32756-32762(2001) [PubMed: 11438518] [Abstract] Cited for: INTERACTION WITH RIMS1.
[12]	"Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly." Scales S.J., Hesser B.A., Masuda E.S., Scheller R.H. J. Biol. Chem. 277:28271-28279(2002) [PubMed: 12145319] [Abstract] Cited for: INTERACTION WITH STXBP6.
[13]	"Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." Sutton R.B., Fasshauer D., Jahn R., Brunger A.T. Nature 395:347-353(1998) [PubMed: 9759724] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-83 AND 120-206 IN COMPLEX WITH STX1A AND VAMP2.
[14]	"Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and syntaxin 1a." Misura K.M.S., Gonzalez L.C. Jr., May A.P., Scheller R.H., Weis W.I. J. Biol. Chem. 276:41301-41309(2001) [PubMed: 11533035] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-83 IN COMPLEX WITH STX1A.
[15]	"High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex." Ernst J.A., Brunger A.T. J. Biol. Chem. 278:8630-8636(2003) [PubMed: 12496247] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 7-83 AND 141-204 IN COMPLEX WITH STX1A AND VAMP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB003991 mRNA. Translation: BAA20151.1.
AB003992 mRNA. Translation: BAA20152.1.
AF245227 mRNA. Translation: AAF81202.1.
U56262 mRNA. Translation: AAA99826.1.
BC087699 mRNA. Translation: AAH87699.1.
U56261 mRNA. Translation: AAA99825.1.

IPI

IPI00204644.
IPI00231420.

RefSeq

NP_112253.1.

UniGene

Rn.107689

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JTH	X-ray	2.00	A/C	1-82	[»]
1N7S	X-ray	1.45	C	7-83	[»]
			D	141-204	[»]
1SFC	X-ray	2.40	C/G/K	1-83	[»]
			D/H/L	120-206	[»]
1URQ	X-ray	2.00	C	7-83	[»]
			D	142-204	[»]
3HD7	X-ray	3.40	C/G	7-83	[»]
			D/H	141-204	[»]
3IPD	X-ray	4.80	C/G	7-83	[»]
			D/H	141-204	[»]

DisProt

DP00068.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:29205N.

IntAct

P60881. 5 interactions.

STRING

P60881.

PTM databases

PhosphoSite

P60881.

Proteomic databases

PRIDE

P60881.

Genome annotation databases

Ensembl

ENSRNOT00000008734; ENSRNOP00000008734; ENSRNOG00000006037; Rattus norvegicus. [Genome view]

GeneID

25012.

KEGG

rno:25012.

Organism-specific databases

CTD

25012.

RGD

3728. Snap25.

Phylogenomic databases

HOVERGEN

P60881.

Gene expression databases

ArrayExpress

P60881.

Genevestigator

P60881.

GermOnline

ENSRNOG00000006037. Rattus norvegicus.

Family and domain databases

InterPro

IPR000928. SNAP-25.
IPR000727. T_SNARE.
[Graphical view]

Pfam

PF00835. SNAP-25. 1 hit.
PF05739. SNARE. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 2 hits.
[Graphical view]

PROSITE

PS50192. T_SNARE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

605093.

PMAP-CutDB

P60881.

Entry information

Entry name

SNP25_RAT

Accession

Primary (citable) accession number: P60881
Secondary accession number(s): P13795 , P36974, P70557, P70558, Q8IXK3, Q96FM2, Q9BR45

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)