##gff-version 3
P60879	UniProtKB	Chain	1	206	.	.	.	ID=PRO_0000213589;Note=Synaptosomal-associated protein 25	
P60879	UniProtKB	Domain	19	81	.	.	.	Note=T-SNARE coiled-coil homology 1;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00202	
P60879	UniProtKB	Domain	140	202	.	.	.	Note=T-SNARE coiled-coil homology 2;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00202	
P60879	UniProtKB	Region	1	75	.	.	.	Note=Interaction with CENPF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672379;Dbxref=PMID:16672379	
P60879	UniProtKB	Region	1	23	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P60879	UniProtKB	Region	111	120	.	.	.	Note=Interaction with ZDHHC17;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60880	
P60879	UniProtKB	Site	180	181	.	.	.	Note=(Microbial infection) Cleavage%3B by C.botulinum neurotoxin type E (BoNT/E);Ontology_term=ECO:0000269,ECO:0000305;evidence=ECO:0000269|PubMed:8243676,ECO:0000305|PubMed:8103915;Dbxref=PMID:8103915,PMID:8243676	
P60879	UniProtKB	Site	197	198	.	.	.	Note=(Microbial infection) Cleavage%3B by C.botulinum neurotoxin type A (BoNT/A%2C botA);Ontology_term=ECO:0000269,ECO:0000305;evidence=ECO:0000269|PubMed:8243676,ECO:0000305|PubMed:8103915;Dbxref=PMID:8103915,PMID:8243676	
P60879	UniProtKB	Modified residue	138	138	.	.	.	Note=Phosphothreonine%3B by PKC and PKA;Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:12459461,ECO:0007744|PubMed:21183079;Dbxref=PMID:12459461,PMID:21183079	
P60879	UniProtKB	Modified residue	154	154	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079	
P60879	UniProtKB	Modified residue	187	187	.	.	.	Note=Phosphoserine%3B by PKC;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12459461;Dbxref=PMID:12459461	
P60879	UniProtKB	Lipidation	85	85	.	.	.	Note=S-palmitoyl cysteine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Lipidation	88	88	.	.	.	Note=S-palmitoyl cysteine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Lipidation	90	90	.	.	.	Note=S-palmitoyl cysteine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Lipidation	92	92	.	.	.	Note=S-palmitoyl cysteine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Alternative sequence	58	89	.	.	.	ID=VSP_010019;Note=In isoform 2. ERIEEGMDQINKDMKEAEKNLTDLGKFCGLCV->DRVEEGMNHINQDMKEAEKNLKDLGKCCGLFI;Ontology_term=ECO:0000303,ECO:0000303,ECO:0000303;evidence=ECO:0000303|PubMed:11471062,ECO:0000303|PubMed:15489334,ECO:0000303|PubMed:2592413;Dbxref=PMID:11471062,PMID:15489334,PMID:2592413	
P60879	UniProtKB	Mutagenesis	85	85	.	.	.	Note=91%25 reduction in palmitoylation level. 14%25 membrane association. No palmitoylation and less than 8%25 membrane association%3B when associated with S-88 or A-88. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Mutagenesis	88	88	.	.	.	Note=79%25 reduction in palmitoylation level. 18%25 membrane association. No palmitoylation and less than 8%25 membrane association%3B when associated with S-85 or A-85. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Mutagenesis	90	90	.	.	.	Note=58%25 reduction in palmitoylation level. 28%25 membrane association. Very little palmitoylation and less than 8%25 membrane association%3B when associated with S-92 or A-92. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529	
P60879	UniProtKB	Mutagenesis	92	92	.	.	.	Note=65%25 reduction in palmitoylation level. 29%25 membrane association. No palmitoylation and less than 8%25 membrane association%3B when associated with S-90 or A-90. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9349529;Dbxref=PMID:9349529