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Protein

Synaptosomal-associated protein 25

Gene

SNAP25

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptosomal-associated protein 25
Short name:
SNAP-25
Alternative name(s):
Synaptosomal-associated 25 kDa protein
Gene namesi
Name:SNAP25
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Chromosome 10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135881 – 206Synaptosomal-associated protein 25Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi85S-palmitoyl cysteineBy similarity1
Lipidationi88S-palmitoyl cysteineBy similarity1
Lipidationi90S-palmitoyl cysteineBy similarity1
Lipidationi92S-palmitoyl cysteineBy similarity1
Modified residuei138PhosphothreonineBy similarity1
Modified residuei154PhosphoserineBy similarity1
Modified residuei187PhosphoserineBy similarity1

Post-translational modificationi

Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei180 – 181Cleavage; by BONT/EBy similarity2

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Expressioni

Gene expression databases

BgeeiENSMMUG00000011064
ExpressionAtlasiP60877 baseline

Interactioni

Subunit structurei

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex binds CPLX1. Found in a complex containing SYT1, SV2B and syntaxin-1. Found in a ternary complex with STX1A and VAMP8. Isoform 1 and isoform 2 interact with BLOC1S6. Interacts with CENPF. Interacts with EQTN. Interacts with HGS. Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. Interacts with OTOF. Interacts with RIMS1. Interacts with SNAPIN. Interacts with STXBP6. Interacts with TRIM9. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Associates with the BLOC-1 complex. Interacts with PLCL1 (via C2 domain). Interacts with PRRT2; this interaction may impair the formation of the SNARE complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000014487

Structurei

3D structure databases

ProteinModelPortaliP60877
SMRiP60877
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 81t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd BLAST63
Domaini140 – 202t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 75Interaction with CENPFBy similarityAdd BLAST75
Regioni111 – 120Interaction with ZDHHC17By similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi85 – 92Cys-rich8

Sequence similaritiesi

Belongs to the SNAP-25 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3065 Eukaryota
ENOG410Y3Y0 LUCA
GeneTreeiENSGT00390000012186
HOVERGENiHBG056971
InParanoidiP60877
KOiK18211
OMAiGEMDENL
TreeFamiTF315125

Family and domain databases

InterProiView protein in InterPro
IPR000928 SNAP-25
IPR000727 T_SNARE_dom
PfamiView protein in Pfam
PF00835 SNAP-25, 1 hit
SMARTiView protein in SMART
SM00397 t_SNARE, 2 hits
PROSITEiView protein in PROSITE
PS50192 T_SNARE, 2 hits

Sequencei

Sequence statusi: Complete.

P60877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML
60 70 80 90 100
DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA
110 120 130 140 150
YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL
160 170 180 190 200
EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT

KMLGSG
Length:206
Mass (Da):23,315
Last modified:April 13, 2004 - v1
Checksum:iFBED2B082A4CB6A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240770 mRNA Translation: AAF64477.1
RefSeqiNP_001028036.1, NM_001032864.1
XP_015004823.1, XM_015149337.1
UniGeneiMmu.442

Genome annotation databases

EnsembliENSMMUT00000015467; ENSMMUP00000014487; ENSMMUG00000011064
GeneIDi574204
KEGGimcc:574204

Similar proteinsi

Entry informationi

Entry nameiSNP25_MACMU
AccessioniPrimary (citable) accession number: P60877
Secondary accession number(s): P13795
, P36974, P70557, P70558, Q8IXK3, Q96FM2, Q9BR45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: April 25, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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