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Protein

Synaptosomal-associated protein 25

Gene

SNAP25

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptosomal-associated protein 25
Short name:
SNAP-25
Alternative name(s):
Synaptosomal-associated 25 kDa protein
Gene namesi
Name:SNAP25
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Chromosome 10

Subcellular locationi

  • Cytoplasmperinuclear region By similarity
  • Cell membrane By similarity; Lipid-anchor By similarity
  • Cell junctionsynapsesynaptosome By similarity

  • Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Synaptosomal-associated protein 25PRO_0000213588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi85 – 851S-palmitoyl cysteineBy similarity
Lipidationi88 – 881S-palmitoyl cysteineBy similarity
Lipidationi90 – 901S-palmitoyl cysteineBy similarity
Lipidationi92 – 921S-palmitoyl cysteineBy similarity
Modified residuei138 – 1381PhosphothreonineBy similarity
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei187 – 1871PhosphoserineBy similarity

Post-translational modificationi

Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei180 – 1812Cleavage; by BONT/EBy similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP60877.

Expressioni

Gene expression databases

ExpressionAtlasiP60877. baseline.

Interactioni

Subunit structurei

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex binds CPLX1 (By similarity). Found in a complex containing SYT1, SV2B and syntaxin-1 (By similarity). Found in a ternary complex with STX1A and VAMP8 (By similarity). Isoform 1 and isoform 2 interact with BLOC1S6 (By similarity). Interacts with CENPF (By similarity). Interacts with EQTN (By similarity). Interacts with HGS (By similarity). Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells (By similarity). Interacts with OTOF (By similarity). Interacts with RIMS1 (By similarity). Interacts with SNAPIN (By similarity). Interacts with STXBP6 (By similarity). Interacts with TRIM9 (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats) (By similarity). Associates with the BLOC-1 complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000014487.

Structurei

3D structure databases

ProteinModelPortaliP60877.
SMRiP60877. Positions 10-78, 141-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 8163t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd
BLAST
Domaini140 – 20263t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7575Interaction with CENPFBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi85 – 928Cys-rich

Sequence similaritiesi

Belongs to the SNAP-25 family.Curated
Contains 2 t-SNARE coiled-coil homology domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
HOVERGENiHBG056971.
InParanoidiP60877.
KOiK18211.
OMAiEGEMDEN.
OrthoDBiEOG75F4F5.
TreeFamiTF315125.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML
60 70 80 90 100
DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA
110 120 130 140 150
YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL
160 170 180 190 200
EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT

KMLGSG
Length:206
Mass (Da):23,315
Last modified:April 13, 2004 - v1
Checksum:iFBED2B082A4CB6A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240770 mRNA. Translation: AAF64477.1.
RefSeqiNP_001028036.1. NM_001032864.1.
XP_015004823.1. XM_015149337.1.
UniGeneiMmu.442.

Genome annotation databases

EnsembliENSMMUT00000015467; ENSMMUP00000014487; ENSMMUG00000011064.
GeneIDi574204.
KEGGimcc:574204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240770 mRNA. Translation: AAF64477.1.
RefSeqiNP_001028036.1. NM_001032864.1.
XP_015004823.1. XM_015149337.1.
UniGeneiMmu.442.

3D structure databases

ProteinModelPortaliP60877.
SMRiP60877. Positions 10-78, 141-204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000014487.

Proteomic databases

PRIDEiP60877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMMUT00000015467; ENSMMUP00000014487; ENSMMUG00000011064.
GeneIDi574204.
KEGGimcc:574204.

Organism-specific databases

CTDi6616.

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
HOVERGENiHBG056971.
InParanoidiP60877.
KOiK18211.
OMAiEGEMDEN.
OrthoDBiEOG75F4F5.
TreeFamiTF315125.

Gene expression databases

ExpressionAtlasiP60877. baseline.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Jensen M.J., Smith L.A.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hippocampus.

Entry informationi

Entry nameiSNP25_MACMU
AccessioniPrimary (citable) accession number: P60877
Secondary accession number(s): P13795
, P36974, P70557, P70558, Q8IXK3, Q96FM2, Q9BR45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.