P60852 (ZP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zona pellucida sperm-binding protein 1

Alternative name(s):
Zona pellucida glycoprotein 1
Short name=Zp-1

Cleaved into the following chain:

1. Processed zona pellucida sperm-binding protein 1

Gene names

Name:

ZP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
Subunit structure	Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.
Subcellular location	Processed zona pellucida sperm-binding protein 1: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Oocytes.
Domain	The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. O-glycosylated By similarity.
Sequence similarities	Belongs to the ZP domain family. ZPB subfamily. Contains 1 P-type (trefoil) domain. Contains 1 ZP domain.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	single fertilization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell secretory granule Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 553	528	Zona pellucida sperm-binding protein 1		PRO_0000041677
	Chain	26 – ?		Processed zona pellucida sperm-binding protein 1		PRO_0000304553
	Propeptide	554 – 638	85	Removed in mature form By similarity		PRO_0000041678
Regions
	Topological domain	26 – 601	576	Extracellular Potential
	Transmembrane	602 – 622	21	Helical; Potential
	Topological domain	623 – 638	16	Cytoplasmic Potential
	Domain	234 – 274	41	P-type
	Domain	279 – 553	275	ZP
Amino acid modifications
	Glycosylation	76	1	N-linked (GlcNAc...) By similarity
	Glycosylation	379	1	N-linked (GlcNAc...) By similarity
	Glycosylation	561	1	N-linked (GlcNAc...) Potential
	Glycosylation	596	1	N-linked (GlcNAc...) Potential
	Disulfide bond	457 ↔ 478		By similarity
Natural variations
	Natural variant	158	1	T → I. Corresponds to variant rs489172 [ dbSNP | Ensembl ].		VAR_052996

Sequences

	Sequence		Length	Mass (Da)
	P60852 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 052107CCFFEDFF21	FASTA	638	70,049
	10 20 30 40 50 60 MAGGSATTWG YPVALLLLVA TLGLGRWLQP DPGLPGLRHS YDCGIKGMQL LVFPRPGQTL 70 80 90 100 110 120 RFKVVDEFGN RFDVNNCSIC YHWVTSRPQE PAVFSADYRG CHVLEKDGRF HLRVFMEAVL 130 140 150 160 170 180 PNGRVDVAQD ATLICPKPDP SRTLDSQLAP PAMFSVSTPQ TLSFLPTSGH TSQGSGHAFP 190 200 210 220 230 240 SPLDPGHSSV HPTPALPSPG PGPTLATLAQ PHWGTLEHWD VNKRDYIGTH LSQEQCQVAS 250 260 270 280 290 300 GHLPCIVRRT SKEACQQAGC CYDNTREVPC YYGNTATVQC FRDGYFVLVV SQEMALTHRI 310 320 330 340 350 360 TLANIHLAYA PTSCSPTQHT EAFVVFYFPL THCGTTMQVA GDQLIYENWL VSGIHIQKGP 370 380 390 400 410 420 QGSITRDSTF QLHVRCVFNA SDFLPIQASI FPPPSPAPMT QPGPLRLELR IAKDETFSSY 430 440 450 460 470 480 YGEDDYPIVR LLREPVHVEV RLLQRTDPNL VLLLHQCWGA PSANPFQQPQ WPILSDGCPF 490 500 510 520 530 540 KGDSYRTQMV ALDGATPFQS HYQRFTVATF ALLDSGSQRA LRGLVYLFCS TSACHTSGLE 550 560 570 580 590 600 TCSTACSTGT TRQRRSSGHR NDTARPQDIV SSPGPVGFED SYGQEPTLGP TDSNGNSSLR 610 620 630 PLLWAVLLLP AVALVLGFGV FVGLSQTWAQ KLWESNRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the true human orthologue of the mouse Zp1 gene: evidence for greater complexity in the mammalian zona pellucida?" Hughes D.C., Barratt C.L. Biochim. Biophys. Acta 1447:303-306(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004126 Genomic DNA. No translation available.
IPI	IPI00410244.
RefSeq	NP_997224.2. NM_207341.2.
UniGene	Hs.172130.
3D structure databases
ProteinModelPortal	P60852.
SMR	P60852. Positions 234-286.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000278853.
PTM databases
PhosphoSite	P60852.
Polymorphism databases
DMDM	46397079.
Proteomic databases
PaxDb	P60852.
PRIDE	P60852.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000278853; ENSP00000278853; ENSG00000149506.
GeneID	22917.
KEGG	hsa:22917.
UCSC	uc001nqd.3. human.
Organism-specific databases
CTD	22917.
GeneCards	GC11P060635.
H-InvDB	HIX0035993.
HGNC	HGNC:13187. ZP1.
HPA	HPA044523.
MIM	195000. gene.
neXtProt	NX_P60852.
PharmGKB	PA37755.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39535.
HOGENOM	HOG000118071.
HOVERGEN	HBG036289.
InParanoid	P60852.
OMA	GCHVLEK.
OrthoDB	EOG4FBHSG.
PhylomeDB	P60852.
Gene expression databases
Bgee	P60852.
CleanEx	HS_ZP1.
Genevestigator	P60852.
GermOnline	ENSG00000149506. Homo sapiens.
Family and domain databases
Gene3D	4.10.110.10. 1 hit.
InterPro	IPR000519. P_trefoil. IPR017957. P_trefoil_CS. IPR001507. ZP_dom. IPR017977. ZP_dom_CS. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00241. ZP. 1 hit. [Graphical view]
SUPFAM	SSF57492. P_trefoil. 1 hit.
PROSITE	PS00025. P_TREFOIL_1. 1 hit. PS51448. P_TREFOIL_2. 1 hit. PS00682. ZP_1. 1 hit. PS51034. ZP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	22917.
NextBio	43609.
SOURCE	Search...

Entry information

Entry name

ZP1_HUMAN

Accession

Primary (citable) accession number: P60852

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.