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Protein

Eukaryotic initiation factor 4A-I

Gene

Eif4a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 83ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1169408 ISG15 antiviral mechanism
R-MMU-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-MMU-72649 Translation initiation complex formation
R-MMU-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-I (EC:3.6.4.13)
Short name:
eIF-4A-I
Short name:
eIF4A-I
Alternative name(s):
ATP-dependent RNA helicase eIF4A-1
Gene namesi
Name:Eif4a1
Synonyms:Ddx2a, Eif4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95303 Eif4a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3309046

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000549352 – 406Eukaryotic initiation factor 4A-IAdd BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Cross-linki146Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei158PhosphothreonineBy similarity1
Modified residuei174N6-acetyllysineBy similarity1
Modified residuei193N6-acetyllysineCombined sources1
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei238N6-acetyllysine; alternateCombined sources1
Cross-linki238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki309Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki369Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki381Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP60843
PaxDbiP60843
PeptideAtlasiP60843
PRIDEiP60843

2D gel databases

REPRODUCTION-2DPAGEP60843

PTM databases

iPTMnetiP60843
PhosphoSitePlusiP60843
SwissPalmiP60843

Expressioni

Gene expression databases

BgeeiENSMUSG00000059796
CleanExiMM_EIF4A1
ExpressionAtlasiP60843 baseline and differential
GenevisibleiP60843 MM

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4 (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi199418, 7 interactors
IntActiP60843, 9 interactors
MINTiP60843
STRINGi10090.ENSMUSP00000127034

Structurei

3D structure databases

ProteinModelPortaliP60843
SMRiP60843
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 234Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini245 – 406Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 60Q motifAdd BLAST29
Motifi182 – 185DEAD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0327 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00530000062880
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiP60843
KOiK03257
OMAiFTHRNGR
OrthoDBiEOG091G07OI
PhylomeDBiP60843
TreeFamiTF101524

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR037583 EIF4A
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PANTHERiPTHR24031:SF226 PTHR24031:SF226, 1 hit
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY
60 70 80 90 100
GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA
110 120 130 140 150
TQALVLAPTR ELAQQIQKVV MALGDYMGAS CHACIGGTNV RAEVQKLQME
160 170 180 190 200
APHIIVGTPG RVFDMLNRRY LSPKYIKMFV LDEADEMLSR GFKDQIYDIF
210 220 230 240 250
QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE LTLEGIRQFY
260 270 280 290 300
INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS
310 320 330 340 350
AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP
360 370 380 390 400
TNRENYIHRI GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL

NVADLI
Length:406
Mass (Da):46,154
Last modified:April 13, 2004 - v1
Checksum:i6EF89939F3045420
GO

Sequence cautioni

The sequence CAA26842 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA26843 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA26845 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA26846 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300S → C in AAA50407 (PubMed:3215517).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03039 mRNA Translation: CAA26842.1 Different initiation.
X03039 mRNA Translation: CAA26843.1 Different initiation.
X03040 mRNA Translation: CAA26845.1 Different initiation.
X03040 mRNA Translation: CAA26846.1 Different initiation.
L36611
, M22873, L36608, L36609, L36610 Genomic DNA Translation: AAA50407.1
AB011595 Genomic DNA Translation: BAA25075.1
AK077429 mRNA Translation: BAC36796.1
BC049915 mRNA Translation: AAH49915.1
CCDSiCCDS24904.1
PIRiJS0039 FIMS4A
RefSeqiNP_659207.1, NM_144958.4
UniGeneiMm.26680
Mm.279821
Mm.371557
Mm.473799

Genome annotation databases

EnsembliENSMUST00000163666; ENSMUSP00000127034; ENSMUSG00000059796
GeneIDi13681
KEGGimmu:13681
UCSCiuc007jra.2 mouse

Similar proteinsi

Entry informationi

Entry nameiIF4A1_MOUSE
AccessioniPrimary (citable) accession number: P60843
Secondary accession number(s): P04765, Q61516
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: April 13, 2004
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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