P60843 (IF4A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic initiation factor 4A-I
Short name=eIF-4A-I
Short name=eIF4A-I
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase eIF4A-1

Gene names

Name:	Eif4a1
Synonyms:	Ddx2a, Eif4a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4 By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. eIF4A subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence CAA26842.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA26843.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA26845.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA26846.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase Initiation factor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 406	405	Eukaryotic initiation factor 4A-I		PRO_0000054935
Regions
	Domain	63 – 234	172	Helicase ATP-binding
	Domain	245 – 406	162	Helicase C-terminal
	Nucleotide binding	76 – 83	8	ATP By similarity
	Motif	32 – 60	29	Q motif
	Motif	182 – 185	4	DEAD box
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	4	1	Phosphoserine By similarity
	Modified residue	118	1	N6-acetyllysine By similarity
	Modified residue	174	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	300	1	S → C in AAA50407. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P60843 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 6EF89939F3045420	FASTA	406	46,154
	10 20 30 40 50 60 MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ 70 80 90 100 110 120 RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA TQALVLAPTR ELAQQIQKVV 130 140 150 160 170 180 MALGDYMGAS CHACIGGTNV RAEVQKLQME APHIIVGTPG RVFDMLNRRY LSPKYIKMFV 190 200 210 220 230 240 LDEADEMLSR GFKDQIYDIF QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE 250 260 270 280 290 300 LTLEGIRQFY INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS 310 320 330 340 350 360 AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP TNRENYIHRI 370 380 390 400 GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL NVADLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A." Nielsen P.J., McMaster G.K., Trachsel H. Nucleic Acids Res. 13:6867-6880(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and mapping of a gene for protein synthesis initiation factor 4A and its expression during differentiation of murine erythroleukemia cells." Reddy N.S., Roth W.W., Bragg P.W., Wahba A.J. Gene 70:231-243(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Miyashita A., Shimizu N., Nakajima T., Odani S., Kuwano R. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[6]	"Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A." Pause A., Somenberg N. EMBO J. 11:2643-2654(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03039 mRNA. Translation: CAA26842.1. Different initiation. X03039 mRNA. Translation: CAA26843.1. Different initiation. X03040 mRNA. Translation: CAA26845.1. Different initiation. X03040 mRNA. Translation: CAA26846.1. Different initiation. L36611 , M22873, L36608, L36609, L36610 Genomic DNA. Translation: AAA50407.1. AB011595 Genomic DNA. Translation: BAA25075.1. AK077429 mRNA. Translation: BAC36796.1. BC049915 mRNA. Translation: AAH49915.1.
IPI	IPI00118676.
PIR	FIMS4A. JS0039.
RefSeq	NP_659207.1. NM_144958.4.
UniGene	Mm.279821. Mm.371557. Mm.473799.
3D structure databases
ProteinModelPortal	P60843.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-202155.
STRING	10090.ENSMUSP00000099649.
PTM databases
PhosphoSite	P60843.
2D gel databases
REPRODUCTION-2DPAGE	P60843.
Proteomic databases
PaxDb	P60843.
PRIDE	P60843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000163666; ENSMUSP00000127034; ENSMUSG00000059796.
GeneID	13681.
KEGG	mmu:13681.
Organism-specific databases
CTD	1973.
MGI	MGI:95303. Eif4a1.
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000268797.
HOVERGEN	HBG107989.
InParanoid	P60843.
KO	K03257.
OMA	IKMFILD.
OrthoDB	EOG4640C1.
Gene expression databases
ArrayExpress	P60843.
Bgee	P60843.
CleanEx	MM_EIF4A1.
Genevestigator	P60843.
GermOnline	ENSMUSG00000064241. Mus musculus.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	284438.
SOURCE	Search...

Entry information

Entry name

IF4A1_MOUSE

Accession

Primary (citable) accession number: P60843
Secondary accession number(s): P04765, Q61516

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program