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Reviewed, UniProtKB/Swiss-Prot P60842 (IF4A1_HUMAN)

Last modified June 16, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Eukaryotic initiation factor 4A-I
      Short name=eIF-4A-I
      Short name=eIF4A-I
    EC=3.6.1.-
Alternative name(s):
    ATP-dependent RNA helicase eIF4A-1
Gene names
Name: EIF4A1
Synonyms: DDX2A, EIF4A
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Subunit structure

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and RENT2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Ref.4 Ref.5 Ref.6 Ref.8

Sequence similarities

Belongs to the DEAD box helicase family. eIF4A subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Eukaryotic initiation factor 4A-I
PRO_0000054933

Regions

Domain63 – 234172Helicase ATP-binding
Domain245 – 406162Helicase C-terminal
Nucleotide binding76 – 838ATP By similarity
Motif32 – 6029Q motif
Motif182 – 1854DEAD box

Amino acid modifications

Modified residue1581Phosphothreonine Ref.9

Secondary structure

.................................... 406
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60842-1 [UniParc].

Last modified April 13, 2004. Version 1.
Checksum: 6EF89939F3045420

FASTA40646,154
        10         20         30         40         50         60 
MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ 

        70         80         90        100        110        120 
RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA TQALVLAPTR ELAQQIQKVV 

       130        140        150        160        170        180 
MALGDYMGAS CHACIGGTNV RAEVQKLQME APHIIVGTPG RVFDMLNRRY LSPKYIKMFV 

       190        200        210        220        230        240 
LDEADEMLSR GFKDQIYDIF QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE 

       250        260        270        280        290        300 
LTLEGIRQFY INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS 

       310        320        330        340        350        360 
AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP TNRENYIHRI 

       370        380        390        400 
GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL NVADLI 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of human cDNA encoding eukaryotic initiation factor 4AI."
Kim N.-S., Kato T., Abe N., Kato S.
Nucleic Acids Res. 21:2012-2012(1993) [PubMed: 8493113] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow and Lung.
[4]"Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation."
Craig A.W.B., Haghighat A., Yu A.T.K., Sonenberg N.
Nature 392:520-523(1998) [PubMed: 9548260] [Abstract]
Cited for: INTERACTION WITH PAIP1.
[5]"Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes."
Mendell J.T., Medghalchi S.M., Lake R.G., Noensie E.N., Dietz H.C.
Mol. Cell. Biol. 20:8944-8957(2000) [PubMed: 11073994] [Abstract]
Cited for: INTERACTION WITH RENT2.
[6]"Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo."
Li W., Belsham G.J., Proud C.G.
J. Biol. Chem. 276:29111-29115(2001) [PubMed: 11408474] [Abstract]
Cited for: INTERACTION WITH EIF4E.
[7]"Exportin 7 defines a novel general nuclear export pathway."
Mingot J.-M., Bohnsack M.T., Jaekle U., Goerlich D.
EMBO J. 23:3227-3236(2004) [PubMed: 15282546] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH XPO7; ARHGAP1; VPS26A; VPS29; VPS35 AND SFN.
[8]"Identification of NOM1, a nucleolar, eIF4A binding protein encoded within the chromosome 7q36 breakpoint region targeted in cases of pediatric acute myeloid leukemia."
Simmons H.M., Ruis B.L., Kapoor M., Hudacek A.W., Conklin K.F.
Gene 347:137-145(2005) [PubMed: 15715967] [Abstract]
Cited for: POSSIBLE INTERACTION WITH NOM1.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-158, MASS SPECTROMETRY.
[10]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

D13748 mRNA. Translation: BAA02897.1.
BT019880 mRNA. Translation: AAV38683.1.
BT019881 mRNA. Translation: AAV38684.1.
BC009585 mRNA. Translation: AAH09585.1.
BC073752 mRNA. Translation: AAH73752.1.
IPIIPI00025491.
PIRS33681.
RefSeqNP_001407.1.
UniGeneHs.129673

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2G9NX-ray2.25A/B20-236[»]
2ZU6X-ray2.80A/C/D/F20-406[»]
3EIQX-ray3.50A/D1-406[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP60842. 16 interactions.

PTM databases

PhosphoSiteP60842.

Proteomic databases

PRIDEP60842.

Genome annotation databases

EnsemblENSG00000161960. Homo sapiens. [Contig view]
GeneID1973.
KEGGhsa:1973.

Organism-specific databases

GeneCardsGC17P007404.
H-InvDBHIX0013502.
HIX0079958.
HGNCHGNC:3282. EIF4A1.
HPACAB011689.
MIM602641. gene.
PharmGKBPA27710.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP60842.
HOVERGENP60842.

Enzyme and pathway databases

Pathway_Interaction_DBmtor_4pathway. mTOR signaling pathway.
ReactomeREACT_1762. 3' -UTR-mediated translational regulation.
REACT_71. Gene Expression.

Gene expression databases

BgeeP60842.
CleanExHS_EIF4A1.
GermOnlineENSG00000161960. Homo sapiens.

Family and domain databases

InterProIPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio7987.
SOURCESearch...

Entry information

Entry nameIF4A1_HUMAN
AccessionPrimary (citable) accession number: P60842
Secondary accession number(s): P04765, Q5U018, Q61516
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: April 13, 2004
Last modified: June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

Translation initiation factors

List of translation initiation factor entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents