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Protein

Eukaryotic initiation factor 4A-I

Gene

EIF4A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Enzyme regulationi

Helicase activity and function in translation are inhibited by interaction with PDCD4.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 83ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • double-stranded RNA binding Source: MGI
  • helicase activity Source: UniProtKB
  • mRNA binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • RNA cap binding Source: UniProtKB
  • translation factor activity, RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Host-virus interaction, Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000161960-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-429947. Deadenylation of mRNA.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-I (EC:3.6.4.13)
Short name:
eIF-4A-I
Short name:
eIF4A-I
Alternative name(s):
ATP-dependent RNA helicase eIF4A-1
Gene namesi
Name:EIF4A1
Synonyms:DDX2A, EIF4A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:3282. EIF4A1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • eukaryotic translation initiation factor 4F complex Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular matrix Source: BHF-UCL
  • membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1973.
OpenTargetsiENSG00000161960.
PharmGKBiPA27710.

Chemistry databases

ChEMBLiCHEMBL2052028.

Polymorphism and mutation databases

BioMutaiEIF4A1.
DMDMi46397463.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000549332 – 406Eukaryotic initiation factor 4A-IAdd BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei4PhosphoserineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei158PhosphothreonineCombined sources1
Modified residuei174N6-acetyllysineCombined sources1
Modified residuei193N6-acetyllysineBy similarity1
Modified residuei238N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP60842.
MaxQBiP60842.
PaxDbiP60842.
PeptideAtlasiP60842.
PRIDEiP60842.
TopDownProteomicsiP60842-1. [P60842-1]

PTM databases

iPTMnetiP60842.
PhosphoSitePlusiP60842.
SwissPalmiP60842.

Expressioni

Gene expression databases

BgeeiENSG00000161960.
CleanExiHS_EIF4A1.
ExpressionAtlasiP60842. baseline and differential.
GenevisibleiP60842. HS.

Organism-specific databases

HPAiCAB011689.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with human cytomegalovirus/HHV-5 protein UL69.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF4G1Q046377EBI-73449,EBI-73711
EIF4G2P783443EBI-73449,EBI-296519
EIF4HQ150562EBI-73449,EBI-748492
PDCD4Q53EL62EBI-73449,EBI-935824

Protein-protein interaction databases

BioGridi108289. 100 interactors.
DIPiDIP-29755N.
IntActiP60842. 50 interactors.
MINTiMINT-5001111.
STRINGi9606.ENSP00000293831.

Chemistry databases

BindingDBiP60842.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 25Combined sources3
Helixi34 – 36Combined sources3
Helixi41 – 50Combined sources10
Helixi57 – 68Combined sources12
Beta strandi72 – 75Combined sources4
Helixi82 – 93Combined sources12
Beta strandi103 – 106Combined sources4
Helixi110 – 124Combined sources15
Turni125 – 128Combined sources4
Beta strandi131 – 134Combined sources4
Turni143 – 145Combined sources3
Beta strandi146 – 149Combined sources4
Beta strandi153 – 157Combined sources5
Helixi159 – 167Combined sources9
Beta strandi178 – 183Combined sources6
Helixi184 – 189Combined sources6
Turni190 – 192Combined sources3
Helixi193 – 202Combined sources10
Beta strandi208 – 214Combined sources7
Helixi218 – 227Combined sources10
Beta strandi232 – 235Combined sources4
Beta strandi241 – 243Combined sources3
Beta strandi246 – 251Combined sources6
Helixi256 – 269Combined sources14
Beta strandi273 – 278Combined sources6
Helixi282 – 290Combined sources9
Turni291 – 296Combined sources6
Beta strandi300 – 302Combined sources3
Beta strandi304 – 306Combined sources3
Helixi312 – 316Combined sources5
Helixi318 – 320Combined sources3
Beta strandi321 – 323Combined sources3
Beta strandi325 – 328Combined sources4
Helixi330 – 333Combined sources4
Helixi338 – 340Combined sources3
Beta strandi342 – 348Combined sources7
Helixi355 – 360Combined sources6
Turni364 – 366Combined sources3
Beta strandi370 – 375Combined sources6
Helixi378 – 391Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G9NX-ray2.25A/B20-238[»]
2ZU6X-ray2.80A/C/D/F20-406[»]
3EIQX-ray3.50A/D1-406[»]
ProteinModelPortaliP60842.
SMRiP60842.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60842.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 234Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini245 – 406Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 60Q motifAdd BLAST29
Motifi182 – 185DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiP60842.
KOiK03257.
OMAiHAQCHAC.
OrthoDBiEOG091G07OI.
PhylomeDBiP60842.
TreeFamiTF101524.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR031258. EIF4A1/2.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF226. PTHR24031:SF226. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60842-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY
60 70 80 90 100
GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA
110 120 130 140 150
TQALVLAPTR ELAQQIQKVV MALGDYMGAS CHACIGGTNV RAEVQKLQME
160 170 180 190 200
APHIIVGTPG RVFDMLNRRY LSPKYIKMFV LDEADEMLSR GFKDQIYDIF
210 220 230 240 250
QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE LTLEGIRQFY
260 270 280 290 300
INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS
310 320 330 340 350
AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP
360 370 380 390 400
TNRENYIHRI GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL

NVADLI
Length:406
Mass (Da):46,154
Last modified:April 13, 2004 - v1
Checksum:i6EF89939F3045420
GO
Isoform 2 (identifier: P60842-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     333-406: ARGIDVQQVS...EMPLNVADLI → GKLYPQNRSRWTVWP

Note: No experimental confirmation available.
Show »
Length:347
Mass (Da):39,548
Checksum:i4ACB22D62DCA8BD0
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046032333 – 406ARGID…VADLI → GKLYPQNRSRWTVWP in isoform 2. 1 PublicationAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13748 mRNA. Translation: BAA02897.1.
BT019880 mRNA. Translation: AAV38683.1.
BT019881 mRNA. Translation: AAV38684.1.
AK312630 mRNA. Translation: BAG35515.1.
AC016876 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90167.1.
CH471108 Genomic DNA. Translation: EAW90168.1.
CH471108 Genomic DNA. Translation: EAW90169.1.
BC006210 mRNA. No translation available.
BC009585 mRNA. Translation: AAH09585.1.
BC073752 mRNA. Translation: AAH73752.1.
CCDSiCCDS11113.1. [P60842-1]
CCDS58511.1. [P60842-2]
PIRiS33681.
RefSeqiNP_001191439.1. NM_001204510.1. [P60842-2]
NP_001407.1. NM_001416.3. [P60842-1]
UniGeneiHs.129673.

Genome annotation databases

EnsembliENST00000293831; ENSP00000293831; ENSG00000161960. [P60842-1]
ENST00000577269; ENSP00000463486; ENSG00000161960. [P60842-2]
GeneIDi1973.
KEGGihsa:1973.
UCSCiuc002ghr.2. human. [P60842-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13748 mRNA. Translation: BAA02897.1.
BT019880 mRNA. Translation: AAV38683.1.
BT019881 mRNA. Translation: AAV38684.1.
AK312630 mRNA. Translation: BAG35515.1.
AC016876 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90167.1.
CH471108 Genomic DNA. Translation: EAW90168.1.
CH471108 Genomic DNA. Translation: EAW90169.1.
BC006210 mRNA. No translation available.
BC009585 mRNA. Translation: AAH09585.1.
BC073752 mRNA. Translation: AAH73752.1.
CCDSiCCDS11113.1. [P60842-1]
CCDS58511.1. [P60842-2]
PIRiS33681.
RefSeqiNP_001191439.1. NM_001204510.1. [P60842-2]
NP_001407.1. NM_001416.3. [P60842-1]
UniGeneiHs.129673.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G9NX-ray2.25A/B20-238[»]
2ZU6X-ray2.80A/C/D/F20-406[»]
3EIQX-ray3.50A/D1-406[»]
ProteinModelPortaliP60842.
SMRiP60842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108289. 100 interactors.
DIPiDIP-29755N.
IntActiP60842. 50 interactors.
MINTiMINT-5001111.
STRINGi9606.ENSP00000293831.

Chemistry databases

BindingDBiP60842.
ChEMBLiCHEMBL2052028.

PTM databases

iPTMnetiP60842.
PhosphoSitePlusiP60842.
SwissPalmiP60842.

Polymorphism and mutation databases

BioMutaiEIF4A1.
DMDMi46397463.

Proteomic databases

EPDiP60842.
MaxQBiP60842.
PaxDbiP60842.
PeptideAtlasiP60842.
PRIDEiP60842.
TopDownProteomicsiP60842-1. [P60842-1]

Protocols and materials databases

DNASUi1973.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293831; ENSP00000293831; ENSG00000161960. [P60842-1]
ENST00000577269; ENSP00000463486; ENSG00000161960. [P60842-2]
GeneIDi1973.
KEGGihsa:1973.
UCSCiuc002ghr.2. human. [P60842-1]

Organism-specific databases

CTDi1973.
DisGeNETi1973.
GeneCardsiEIF4A1.
HGNCiHGNC:3282. EIF4A1.
HPAiCAB011689.
MIMi602641. gene.
neXtProtiNX_P60842.
OpenTargetsiENSG00000161960.
PharmGKBiPA27710.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiP60842.
KOiK03257.
OMAiHAQCHAC.
OrthoDBiEOG091G07OI.
PhylomeDBiP60842.
TreeFamiTF101524.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000161960-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-429947. Deadenylation of mRNA.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

ChiTaRSiEIF4A1. human.
EvolutionaryTraceiP60842.
GeneWikiiEIF4A1.
GenomeRNAii1973.
PROiP60842.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161960.
CleanExiHS_EIF4A1.
ExpressionAtlasiP60842. baseline and differential.
GenevisibleiP60842. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR031258. EIF4A1/2.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PANTHERiPTHR24031:SF226. PTHR24031:SF226. 1 hit.
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4A1_HUMAN
AccessioniPrimary (citable) accession number: P60842
Secondary accession number(s): B2R6L8
, D3DTP9, J3QLC4, P04765, Q5U018, Q61516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: April 13, 2004
Last modified: November 2, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.