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Protein

Alpha-endosulfine

Gene

Ensa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-2465910. MASTL Facilitates Mitotic Progression.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-endosulfine
Alternative name(s):
ARPP-19e
Gene namesi
Name:Ensa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi62007. Ensa.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001467612 – 121Alpha-endosulfineAdd BLAST120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Modified residuei21PhosphothreonineBy similarity1
Modified residuei43PhosphoserineBy similarity1
Modified residuei67Phosphoserine; by GWLBy similarity1
Modified residuei109Phosphoserine; by PKA1 Publication1

Post-translational modificationi

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A. Phosphorylated by PKA (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP60841.
PRIDEiP60841.

PTM databases

iPTMnetiP60841.
PhosphoSitePlusiP60841.

Expressioni

Tissue specificityi

Present in brain (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000048617.
GenevisibleiP60841. RN.

Interactioni

Subunit structurei

Interacts (when phosphorylated at Ser-67) with PPP2R2D. Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063946.

Structurei

3D structure databases

ProteinModelPortaliP60841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the endosulfine family.Curated

Phylogenomic databases

eggNOGiKOG4076. Eukaryota.
ENOG4112586. LUCA.
GeneTreeiENSGT00390000010139.
HOVERGENiHBG000297.
InParanoidiP60841.
OMAiEQKYFDS.
OrthoDBiEOG091G14MO.
PhylomeDBiP60841.

Family and domain databases

InterProiIPR006760. Endosulphine.
[Graphical view]
PfamiPF04667. Endosulfine. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60841-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQKQEEENP AEETGEEKQD TQEKEGILPE KAEEAKLKAK YPSLGQKPGG
60 70 80 90 100
SDFLMKRLQK GQKYFDSGDY NMAKAKMKNK QLPSAGADKN LVTGDHIPTP
110 120
QDLPQRKSSL VTSKLAGGQV E
Length:121
Mass (Da):13,335
Last modified:April 13, 2004 - v1
Checksum:iA84632C9085FFC5A
GO
Isoform 2 (identifier: P60841-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-121: Missing.

Show »
Length:117
Mass (Da):12,922
Checksum:iB85FFC5A907676C6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037070118 – 121Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005984 mRNA. Translation: CAA06798.1.
BC059135 mRNA. Translation: AAH59135.1.
RefSeqiNP_001029146.1. NM_001033974.2. [P60841-2]
NP_068614.1. NM_021842.3. [P60841-1]
UniGeneiRn.231694.
Rn.27154.

Genome annotation databases

EnsembliENSRNOT00000075511; ENSRNOP00000063946; ENSRNOG00000048617. [P60841-1]
ENSRNOT00000079196; ENSRNOP00000070245; ENSRNOG00000048617. [P60841-2]
GeneIDi60334.
KEGGirno:60334.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005984 mRNA. Translation: CAA06798.1.
BC059135 mRNA. Translation: AAH59135.1.
RefSeqiNP_001029146.1. NM_001033974.2. [P60841-2]
NP_068614.1. NM_021842.3. [P60841-1]
UniGeneiRn.231694.
Rn.27154.

3D structure databases

ProteinModelPortaliP60841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063946.

PTM databases

iPTMnetiP60841.
PhosphoSitePlusiP60841.

Proteomic databases

PaxDbiP60841.
PRIDEiP60841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000075511; ENSRNOP00000063946; ENSRNOG00000048617. [P60841-1]
ENSRNOT00000079196; ENSRNOP00000070245; ENSRNOG00000048617. [P60841-2]
GeneIDi60334.
KEGGirno:60334.

Organism-specific databases

CTDi2029.
RGDi62007. Ensa.

Phylogenomic databases

eggNOGiKOG4076. Eukaryota.
ENOG4112586. LUCA.
GeneTreeiENSGT00390000010139.
HOVERGENiHBG000297.
InParanoidiP60841.
OMAiEQKYFDS.
OrthoDBiEOG091G14MO.
PhylomeDBiP60841.

Enzyme and pathway databases

ReactomeiR-RNO-2465910. MASTL Facilitates Mitotic Progression.

Miscellaneous databases

PROiP60841.

Gene expression databases

BgeeiENSRNOG00000048617.
GenevisibleiP60841. RN.

Family and domain databases

InterProiIPR006760. Endosulphine.
[Graphical view]
PfamiPF04667. Endosulfine. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENSA_RAT
AccessioniPrimary (citable) accession number: P60841
Secondary accession number(s): Q6PCU7, Q9CQZ9, Q9Z0G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.